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The map label for this gene is llaDCHIA [H]

Identifier: 86605658

GI number: 86605658

Start: 969244

End: 970194

Strand: Reverse

Name: llaDCHIA [H]

Synonym: CYA_0956

Alternate gene names: 86605658

Gene position: 970194-969244 (Counterclockwise)

Preceding gene: 86605659

Following gene: 86605656

Centisome position: 33.08

GC content: 47.11

Gene sequence:

>951_bases
ATGAGCGATTTGCTTCTTCCGCGACCTTTTCTAAAATGGGCCGGTGGCAAGACACAACTGACAGACGCCCTATTAGAGCG
GATGCCGGTCTATTTCAGAACTTACCACGAGCCTTTTGTTGGGAGTGGCGCGCTTTTCTTTCGCCTGTATAGAGAATGCA
AAATCAAACAGGCGATCCTTTCCGATATCAACGCTGAGCTGATAGATACTTACCTGGCTATTCGCGATCATGTTTGGGAA
GTTATTCAACTACTCTCCGAATTTCCACACAATGAAAGTTTTTATTACCAGATTCGAGAGAAAGATCCTTGGTCAATGGG
TCTTGTAGGACGAGCAGCGCGCATGATTTACCTGAACAAGACAGGCTATAATGGTTTGTATCGCGTCAATCGGCAAGGAA
AGTTCAATGTTCCTTTTGGGCGATATAAAGCTCCAAAGTATCTAGACAAGGACAATCTTTTAGCTGCATCCCTTGCTCTA
CAAAATGTTGAAATTTTGTGCGTGCCATTTGATACGGTTGTTGAGAGGGCCAAGCCAGGCGACTGGGTGTATTTCGACCC
GCCTTACGTTCCTGTCTCGCAAACATCAAACTTTACCTCCTATCATGCCAGTGGCTTCGGCCTAGAGGATCAAGAGCGAT
TACGAGACATTTGCATTGACTTAAGCAAGAATAAGGTGTACATCATGCTTTCTAATTCTGACACCGCAATCATTCGCTCT
TTGTACGCTATGCCCTTCTTTGCTATTGATGAAGTTCAAGCAAACCGGGCTATCAACTGCAAGGGGACAAAACGAGGCAA
AATCACGGAGCTAGTGATCACAAACTACCCTGTTGAAAGAGCGGTGCAGCTAGGCTTACTCGATCAGGGGTTGCCTTATT
TGAGGGCCACCCAACACTCGCTTGCGCCTGCTGTCACTCCGCTGCGCTTCGCGGGGCAGGTGAGATCCTGA

Upstream 100 bases:

>100_bases
GGGTTTTCAAAGAACATGCAAGGATAACCGATGTCTACTGAGAAAGTGGTTTGGTTCAGCGATTTAGAAGATTGGTTACG
TTTGTATTTTGGATTGTAAC

Downstream 100 bases:

>100_bases
GGCAGTCGACTAGTAGGTGCGCCCGCGCCAAGAGCGGGGGCGGCTGAGGGCTGACAAGATCACCCGCAGGACAGCCAAGG
GATCCGCCAGCGGCGAAGCC

Product: D12 class N6 adenine-specific DNA methyltransferase

Products: NA

Alternate protein names: M.LlaDCHI A; M.LlaDCHIA; Adenine-specific methyltransferase LlaDCHIA; M.LlaII A [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSDLLLPRPFLKWAGGKTQLTDALLERMPVYFRTYHEPFVGSGALFFRLYRECKIKQAILSDINAELIDTYLAIRDHVWE
VIQLLSEFPHNESFYYQIREKDPWSMGLVGRAARMIYLNKTGYNGLYRVNRQGKFNVPFGRYKAPKYLDKDNLLAASLAL
QNVEILCVPFDTVVERAKPGDWVYFDPPYVPVSQTSNFTSYHASGFGLEDQERLRDICIDLSKNKVYIMLSNSDTAIIRS
LYAMPFFAIDEVQANRAINCKGTKRGKITELVITNYPVERAVQLGLLDQGLPYLRATQHSLAPAVTPLRFAGQVRS

Sequences:

>Translated_316_residues
MSDLLLPRPFLKWAGGKTQLTDALLERMPVYFRTYHEPFVGSGALFFRLYRECKIKQAILSDINAELIDTYLAIRDHVWE
VIQLLSEFPHNESFYYQIREKDPWSMGLVGRAARMIYLNKTGYNGLYRVNRQGKFNVPFGRYKAPKYLDKDNLLAASLAL
QNVEILCVPFDTVVERAKPGDWVYFDPPYVPVSQTSNFTSYHASGFGLEDQERLRDICIDLSKNKVYIMLSNSDTAIIRS
LYAMPFFAIDEVQANRAINCKGTKRGKITELVITNYPVERAVQLGLLDQGLPYLRATQHSLAPAVTPLRFAGQVRS
>Mature_315_residues
SDLLLPRPFLKWAGGKTQLTDALLERMPVYFRTYHEPFVGSGALFFRLYRECKIKQAILSDINAELIDTYLAIRDHVWEV
IQLLSEFPHNESFYYQIREKDPWSMGLVGRAARMIYLNKTGYNGLYRVNRQGKFNVPFGRYKAPKYLDKDNLLAASLALQ
NVEILCVPFDTVVERAKPGDWVYFDPPYVPVSQTSNFTSYHASGFGLEDQERLRDICIDLSKNKVYIMLSNSDTAIIRSL
YAMPFFAIDEVQANRAINCKGTKRGKITELVITNYPVERAVQLGLLDQGLPYLRATQHSLAPAVTPLRFAGQVRS

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=269, Percent_Identity=37.9182156133829, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 36153; Mature: 36022

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLLPRPFLKWAGGKTQLTDALLERMPVYFRTYHEPFVGSGALFFRLYRECKIKQAIL
CCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
SDINAELIDTYLAIRDHVWEVIQLLSEFPHNESFYYQIREKDPWSMGLVGRAARMIYLNK
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCEEEEEEEEC
TGYNGLYRVNRQGKFNVPFGRYKAPKYLDKDNLLAASLALQNVEILCVPFDTVVERAKPG
CCCCCEEEECCCCEECCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECHHHHHHHCCCC
DWVYFDPPYVPVSQTSNFTSYHASGFGLEDQERLRDICIDLSKNKVYIMLSNSDTAIIRS
CEEEECCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LYAMPFFAIDEVQANRAINCKGTKRGKITELVITNYPVERAVQLGLLDQGLPYLRATQHS
HHHCCCCEEHHHHCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHH
LAPAVTPLRFAGQVRS
HCCCCCHHHHCCCCCC
>Mature Secondary Structure 
SDLLLPRPFLKWAGGKTQLTDALLERMPVYFRTYHEPFVGSGALFFRLYRECKIKQAIL
CCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
SDINAELIDTYLAIRDHVWEVIQLLSEFPHNESFYYQIREKDPWSMGLVGRAARMIYLNK
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCEEEEEEEEC
TGYNGLYRVNRQGKFNVPFGRYKAPKYLDKDNLLAASLALQNVEILCVPFDTVVERAKPG
CCCCCEEEECCCCEECCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECHHHHHHHCCCC
DWVYFDPPYVPVSQTSNFTSYHASGFGLEDQERLRDICIDLSKNKVYIMLSNSDTAIIRS
CEEEECCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LYAMPFFAIDEVQANRAINCKGTKRGKITELVITNYPVERAVQLGLLDQGLPYLRATQHS
HHHCCCCEEHHHHCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHH
LAPAVTPLRFAGQVRS
HCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939; 11467810 [H]