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The map label for this gene is ntrB-2

Identifier: 86605595

GI number: 86605595

Start: 900649

End: 901476

Strand: Reverse

Name: ntrB-2

Synonym: CYA_0893

Alternate gene names: 86605595

Gene position: 901476-900649 (Counterclockwise)

Preceding gene: 86605596

Following gene: 86605594

Centisome position: 30.74

GC content: 52.78

Gene sequence:

>828_bases
ATGACGACGACACTTTCTTCTCCGGCAAAGTCTTCGGCAGCGAAGCTCGATTTGGCGAAGTACCGAGATCAGATTCTCCC
CCCTGTGGTGGGTATTGTTGGATTTCTGCTCATCTGGCAGATCTTGGCCAGTGCCAACCTAATTCGGCTGCCGGGGCCTT
GGGATGTGTGGATGGATGAGCGCAGCCGATATCTGATCCTGCATCCTTTGTATCGCAACAGCGACCTCGACCAAGGGCTG
CTGCGCCAAACCTTGGCCAGCCTACAAAGGGTGGCCATCGGCTATTGCAGTGCCGCTTTGGTGGGTATCACGTTGGGCAT
TTTGGTGGGAAGCAGTGTGTGGATCAACCGAGCTTTGGATCCCATCTTTCAATTCTTGCGCATGGTAGCTCCCTTAGCTT
GGGTGCCCATTTCCTTGGCTGCTTCGCAGCGCAATGAGCCGGCTGCCCTGTTTGTTATTTTTATCACCTCAATTTGGCCC
ATTCTCATCAACACGGCTGTGGGGGTGAGACAGATCCCGGAAGACTACATCAACGTCTCCAAAGTGCTCAAGCTCTCCAA
AGTAGACTTCTTTTTTAAGGTTTTGCTGCCTTCGGTTCTGCCCTACATGTTCACAGGTCTGCGCATTGGTATTGGCCTAT
CTTGGCTGGCGATTATTGCCGCAGAAATTGTCATGTCCGGCGTGCCAGGGATCGGCTTTTTCATCTGGGATGCCTACCAG
CAGAACTACGTGACTGAGATCATCGTTGCTGTCATCTACATTGGGGCGGTGGGCTTGCTTTTGGATCGACTGGTGGCTGC
CCTGCAAAACAAAATAGCCAATCGCTAG

Upstream 100 bases:

>100_bases
TCATGCTCTGTTTCCAACACGTTGTAGAACCCTGCGGCTGGAATGTTCTGAGAGCGCGACAACAGAGAACCTTGTAAAAC
CCTATCCTCCTCTAGGAGCT

Downstream 100 bases:

>100_bases
CCTCGTTTGCAGATCTTTTCACTGCATGTCCATCCAATTGAGTTGGGATCCCTCTTACATTGTCTTTGAGTGTTAGCCCC
AATCAGCTCTTCAAAAAGAG

Product: nitrate ABC transporter, permease protein

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTTTLSSPAKSSAAKLDLAKYRDQILPPVVGIVGFLLIWQILASANLIRLPGPWDVWMDERSRYLILHPLYRNSDLDQGL
LRQTLASLQRVAIGYCSAALVGITLGILVGSSVWINRALDPIFQFLRMVAPLAWVPISLAASQRNEPAALFVIFITSIWP
ILINTAVGVRQIPEDYINVSKVLKLSKVDFFFKVLLPSVLPYMFTGLRIGIGLSWLAIIAAEIVMSGVPGIGFFIWDAYQ
QNYVTEIIVAVIYIGAVGLLLDRLVAALQNKIANR

Sequences:

>Translated_275_residues
MTTTLSSPAKSSAAKLDLAKYRDQILPPVVGIVGFLLIWQILASANLIRLPGPWDVWMDERSRYLILHPLYRNSDLDQGL
LRQTLASLQRVAIGYCSAALVGITLGILVGSSVWINRALDPIFQFLRMVAPLAWVPISLAASQRNEPAALFVIFITSIWP
ILINTAVGVRQIPEDYINVSKVLKLSKVDFFFKVLLPSVLPYMFTGLRIGIGLSWLAIIAAEIVMSGVPGIGFFIWDAYQ
QNYVTEIIVAVIYIGAVGLLLDRLVAALQNKIANR
>Mature_274_residues
TTTLSSPAKSSAAKLDLAKYRDQILPPVVGIVGFLLIWQILASANLIRLPGPWDVWMDERSRYLILHPLYRNSDLDQGLL
RQTLASLQRVAIGYCSAALVGITLGILVGSSVWINRALDPIFQFLRMVAPLAWVPISLAASQRNEPAALFVIFITSIWPI
LINTAVGVRQIPEDYINVSKVLKLSKVDFFFKVLLPSVLPYMFTGLRIGIGLSWLAIIAAEIVMSGVPGIGFFIWDAYQQ
NYVTEIIVAVIYIGAVGLLLDRLVAALQNKIANR

Specific function: Part of the ABC transporter complex CmpABCD involved in bicarbonate transport. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0600

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=197, Percent_Identity=29.4416243654822, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081802, Length=186, Percent_Identity=34.4086021505376, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30362; Mature: 30230

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTTLSSPAKSSAAKLDLAKYRDQILPPVVGIVGFLLIWQILASANLIRLPGPWDVWMDE
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHCC
RSRYLILHPLYRNSDLDQGLLRQTLASLQRVAIGYCSAALVGITLGILVGSSVWINRALD
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
PIFQFLRMVAPLAWVPISLAASQRNEPAALFVIFITSIWPILINTAVGVRQIPEDYINVS
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KVLKLSKVDFFFKVLLPSVLPYMFTGLRIGIGLSWLAIIAAEIVMSGVPGIGFFIWDAYQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QNYVTEIIVAVIYIGAVGLLLDRLVAALQNKIANR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]