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The map label for this gene is nth [H]

Identifier: 86605550

GI number: 86605550

Start: 855551

End: 856219

Strand: Reverse

Name: nth [H]

Synonym: CYA_0846

Alternate gene names: 86605550

Gene position: 856219-855551 (Counterclockwise)

Preceding gene: 86605551

Following gene: 86605545

Centisome position: 29.19

GC content: 63.08

Gene sequence:

>669_bases
GTGCTGGAATCACTGTTGGCGCGCCTGGAACAGTGCTTTGGCATCCCCCAACGTCCTCGCAGCCCTGATGGTCTGCCCCT
CTATCCCGACCTCTTGGACGAATTGGTGGGCACGATCCTCTCCCAGAACACCACCGACCACAATAGCAGCCGCGCCTTTC
GCGCCCTCAAAGCCGCTTTCCCCTCCTGGGAAGCTGTCTTAGCTGCTGATCCAACCAAGCTGGCCCAGGTGATCCGTCCC
GGGGGCCTGGCCCAACTCAAAGCAGCCCGCATTCAGGAGATTCTGGCTGCCATTGTCAAGCGACAGGGATCCCTGAGCCT
GGACTTTTTGCGGGATCTGGACGACGCGGACGCTCTGGCCTATCTCCTTTCCCTGAAGGGGGTAGGACTGAAGACAGCCG
CCTGCGTGCTGTTGTTTGGCCTGGGCCGGGATCTCTGCCCGGTGGATACCCATGTGCATCGGGTGGCCAACCGTCTTGGC
TTGGTGCGGGCCAGACACCCCGACGACACCTTTGCCCAACTGTCGCCGCGGATCCCGGCGGGCAAGGCCTATTCCCTGCA
CGTCAACTTGATCCGGCTGGGCAAACGCATCTGTAAGGCGCGCATGCCCGAATGTGGCCGCTGCCCTCTGCGACACGAAT
GCCCCTCCGCCCTGCTGCGGCAGCGCTGA

Upstream 100 bases:

>100_bases
TCGGGATCCTCTTGCCCGCCGCCTGCCTGAGCCTGCCGCAGCCATGGAGACTTCAGCCTGGCTGGAACCTCGTCCTGGTG
AGGCGAGCCCCAACGCCGTC

Downstream 100 bases:

>100_bases
TCCCTACTCTGCCGGCGCAGGGGAAGAGGGAGTGGGCTGGGCAGGGATCCCGTTTTCGACCCCACCTGGGGCAGGAACTT
CCGGCTCGAGGACTTGCCGC

Product: base excision DNA repair protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MLESLLARLEQCFGIPQRPRSPDGLPLYPDLLDELVGTILSQNTTDHNSSRAFRALKAAFPSWEAVLAADPTKLAQVIRP
GGLAQLKAARIQEILAAIVKRQGSLSLDFLRDLDDADALAYLLSLKGVGLKTAACVLLFGLGRDLCPVDTHVHRVANRLG
LVRARHPDDTFAQLSPRIPAGKAYSLHVNLIRLGKRICKARMPECGRCPLRHECPSALLRQR

Sequences:

>Translated_222_residues
MLESLLARLEQCFGIPQRPRSPDGLPLYPDLLDELVGTILSQNTTDHNSSRAFRALKAAFPSWEAVLAADPTKLAQVIRP
GGLAQLKAARIQEILAAIVKRQGSLSLDFLRDLDDADALAYLLSLKGVGLKTAACVLLFGLGRDLCPVDTHVHRVANRLG
LVRARHPDDTFAQLSPRIPAGKAYSLHVNLIRLGKRICKARMPECGRCPLRHECPSALLRQR
>Mature_222_residues
MLESLLARLEQCFGIPQRPRSPDGLPLYPDLLDELVGTILSQNTTDHNSSRAFRALKAAFPSWEAVLAADPTKLAQVIRP
GGLAQLKAARIQEILAAIVKRQGSLSLDFLRDLDDADALAYLLSLKGVGLKTAACVLLFGLGRDLCPVDTHVHRVANRLG
LVRARHPDDTFAQLSPRIPAGKAYSLHVNLIRLGKRICKARMPECGRCPLRHECPSALLRQR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=186, Percent_Identity=28.494623655914, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787920, Length=180, Percent_Identity=27.7777777777778, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=188, Percent_Identity=31.9148936170213, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45550361, Length=194, Percent_Identity=28.3505154639175, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24364; Mature: 24364

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESLLARLEQCFGIPQRPRSPDGLPLYPDLLDELVGTILSQNTTDHNSSRAFRALKAAF
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
PSWEAVLAADPTKLAQVIRPGGLAQLKAARIQEILAAIVKRQGSLSLDFLRDLDDADALA
CCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHH
YLLSLKGVGLKTAACVLLFGLGRDLCPVDTHVHRVANRLGLVRARHPDDTFAQLSPRIPA
HHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC
GKAYSLHVNLIRLGKRICKARMPECGRCPLRHECPSALLRQR
CCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MLESLLARLEQCFGIPQRPRSPDGLPLYPDLLDELVGTILSQNTTDHNSSRAFRALKAAF
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
PSWEAVLAADPTKLAQVIRPGGLAQLKAARIQEILAAIVKRQGSLSLDFLRDLDDADALA
CCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHH
YLLSLKGVGLKTAACVLLFGLGRDLCPVDTHVHRVANRLGLVRARHPDDTFAQLSPRIPA
HHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC
GKAYSLHVNLIRLGKRICKARMPECGRCPLRHECPSALLRQR
CCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]