Click here to switch to the map view.

The map label for this gene is purD

Identifier: 86605337

GI number: 86605337

Start: 631635

End: 632930

Strand: Reverse

Name: purD

Synonym: CYA_0622

Alternate gene names: 86605337

Gene position: 632930-631635 (Counterclockwise)

Preceding gene: 86605338

Following gene: 86605336

Centisome position: 21.58

GC content: 63.66

Gene sequence:

>1296_bases
ATGCAGGCTTTGGTCATCGGCTCCGGGGGACGGGAACACGCTCTGGCCTGGAAGCTGCTGCAGTCTCCCCAGATTACTCG
GGTCTACTGGGCACCTGGCAACGGCGGCGCCCAGCAGATGCCCCGCTGCCAATCAGTGGCTTTGGCGGCCACCGACATCG
AGGGGCTGGTGCGATTTGCTACCGTGCAGGGGATCCGCCTGACGGTGGTGGGGCCGGAGCTGCCCCTGGTGGAGGGAATT
GTGGACGCCTTTCAGGCAGCGGGGCTGCCCATTTTTGGCCCCAGTCGCGCCGCCGCCCAACTGGAGGGAAGCAAGCTCTG
GGCCAAGCAACTGATGCAGGAAGCAGGGATCCCTACGGCTCCGGCGGTAGGCTTTGCCGACTTGTCGGAGGCGCTGGCCC
ACCTGAATTCTCGCAGCTACCCCGTGGTGATCAAAAAAGATGGGCTGGCTGCCGGCAAGGGGGTAACCGTTGCCCAAAAC
CGGGAAGAAGCCGTGCAAGCCCTGGAAGCAGCCCTGGCCAAGCCGGGCGATCAGGTGCTGATCGAGGAGTTTATCCCCGG
CCAGGAGGTGTCGGTTTTGGCTCTAACCGATGGCCAGATCTGTGTGCCGCTGCCACCTGCCCAGGACTACAAGCGCCTTG
GCGAGGGCGATACTGGCCCCAACACCGGCGGCATGGGAGCTCATGCCCCTGCGGATCACCTGGTCACTCCAGAAGGGTTG
GCCCGCATCCAAACCCAGATCCTGGATCCCACCCTGGAGGCTTTGCAACGGCGGGGCATCCGCTACCAGGGGGTGATCTA
TGCCGGTTTGATGATCACGCCCAGTGGGGATCCCTACGTGTTGGAGTACAACTGCCGCTTTGGGGATCCAGAGGCTCAAG
TCCTCCTGCCCTTGCTGGAAAACCCTTTGGACGAGGTGCTGCTGGCCTGTATAGAAGGACGGCTGGCCCAAATCCAGCTC
AAATGGAAACCCGGCTATGCCGCCTGCGTGGTGATGGCAGCCCCAGGCTATCCCGATGCCTACGAGCGAGGGATCCCCAT
CTCCGGCTTGGATGCAGCTGCCACCACGGGGGCGTTGGTGTTTCATGCGGGTACCCGCCAGGCAGGCAAGCAGCTCCTGA
GCGATGGCGGGCGCGTTCTCTGTCTTACCGGCCAAGGGGCAACCCTATCCGAGGCCCTGCGCCAAGCCTACGCAGCTGTG
GAGCAAGTGCAGTTTCCCAAGGCCTACTACCGTTTTGACATCGGCAGCCACGCCCTGGGGCCTGCCGTCCGCCGAGGAGT
GATCCCTACCCCATAA

Upstream 100 bases:

>100_bases
AACATGGTCTAATGGGGAATGTTCGGGAAGGATCCCAAAATAACCAAGCGGCGTTTACAAGAGCGGCGTTTACAAGGCGT
TTACAAAGTGAGATAGCACA

Downstream 100 bases:

>100_bases
AGCCATCCCTTGCCCTCTCAGCTGCAGCCGGATGGAAGAAGCCCCAGAGGGGTCGCTCCTCCAGGGCCCATAAAGCAGCT
TTGAAAAAAGCTTTGATGCT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQALVIGSGGREHALAWKLLQSPQITRVYWAPGNGGAQQMPRCQSVALAATDIEGLVRFATVQGIRLTVVGPELPLVEGI
VDAFQAAGLPIFGPSRAAAQLEGSKLWAKQLMQEAGIPTAPAVGFADLSEALAHLNSRSYPVVIKKDGLAAGKGVTVAQN
REEAVQALEAALAKPGDQVLIEEFIPGQEVSVLALTDGQICVPLPPAQDYKRLGEGDTGPNTGGMGAHAPADHLVTPEGL
ARIQTQILDPTLEALQRRGIRYQGVIYAGLMITPSGDPYVLEYNCRFGDPEAQVLLPLLENPLDEVLLACIEGRLAQIQL
KWKPGYAACVVMAAPGYPDAYERGIPISGLDAAATTGALVFHAGTRQAGKQLLSDGGRVLCLTGQGATLSEALRQAYAAV
EQVQFPKAYYRFDIGSHALGPAVRRGVIPTP

Sequences:

>Translated_431_residues
MQALVIGSGGREHALAWKLLQSPQITRVYWAPGNGGAQQMPRCQSVALAATDIEGLVRFATVQGIRLTVVGPELPLVEGI
VDAFQAAGLPIFGPSRAAAQLEGSKLWAKQLMQEAGIPTAPAVGFADLSEALAHLNSRSYPVVIKKDGLAAGKGVTVAQN
REEAVQALEAALAKPGDQVLIEEFIPGQEVSVLALTDGQICVPLPPAQDYKRLGEGDTGPNTGGMGAHAPADHLVTPEGL
ARIQTQILDPTLEALQRRGIRYQGVIYAGLMITPSGDPYVLEYNCRFGDPEAQVLLPLLENPLDEVLLACIEGRLAQIQL
KWKPGYAACVVMAAPGYPDAYERGIPISGLDAAATTGALVFHAGTRQAGKQLLSDGGRVLCLTGQGATLSEALRQAYAAV
EQVQFPKAYYRFDIGSHALGPAVRRGVIPTP
>Mature_431_residues
MQALVIGSGGREHALAWKLLQSPQITRVYWAPGNGGAQQMPRCQSVALAATDIEGLVRFATVQGIRLTVVGPELPLVEGI
VDAFQAAGLPIFGPSRAAAQLEGSKLWAKQLMQEAGIPTAPAVGFADLSEALAHLNSRSYPVVIKKDGLAAGKGVTVAQN
REEAVQALEAALAKPGDQVLIEEFIPGQEVSVLALTDGQICVPLPPAQDYKRLGEGDTGPNTGGMGAHAPADHLVTPEGL
ARIQTQILDPTLEALQRRGIRYQGVIYAGLMITPSGDPYVLEYNCRFGDPEAQVLLPLLENPLDEVLLACIEGRLAQIQL
KWKPGYAACVVMAAPGYPDAYERGIPISGLDAAATTGALVFHAGTRQAGKQLLSDGGRVLCLTGQGATLSEALRQAYAAV
EQVQFPKAYYRFDIGSHALGPAVRRGVIPTP

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=44.9411764705882, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=44.9411764705882, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=44.9411764705882, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=44.9411764705882, Blast_Score=353, Evalue=2e-97,
Organism=Escherichia coli, GI1790438, Length=425, Percent_Identity=47.0588235294118, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17567511, Length=429, Percent_Identity=40.3263403263403, Blast_Score=302, Evalue=2e-82,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=40.9090909090909, Blast_Score=310, Evalue=4e-85,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=46.4037122969838, Blast_Score=338, Evalue=6e-93,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=46.4037122969838, Blast_Score=337, Evalue=6e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45454; Mature: 45454

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALVIGSGGREHALAWKLLQSPQITRVYWAPGNGGAQQMPRCQSVALAATDIEGLVRFA
CCEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHCCCHHHHEEEHHHHHHHHHHH
TVQGIRLTVVGPELPLVEGIVDAFQAAGLPIFGPSRAAAQLEGSKLWAKQLMQEAGIPTA
EECCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCC
PAVGFADLSEALAHLNSRSYPVVIKKDGLAAGKGVTVAQNREEAVQALEAALAKPGDQVL
CCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHH
IEEFIPGQEVSVLALTDGQICVPLPPAQDYKRLGEGDTGPNTGGMGAHAPADHLVTPEGL
HHHCCCCCCEEEEEEECCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
ARIQTQILDPTLEALQRRGIRYQGVIYAGLMITPSGDPYVLEYNCRFGDPEAQVLLPLLE
HHHHHHHHCHHHHHHHHCCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCHHHEEHHHHH
NPLDEVLLACIEGRLAQIQLKWKPGYAACVVMAAPGYPDAYERGIPISGLDAAATTGALV
CCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCHHHHCCCCCCCCCHHHHCCEEE
FHAGTRQAGKQLLSDGGRVLCLTGQGATLSEALRQAYAAVEQVQFPKAYYRFDIGSHALG
EECCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCC
PAVRRGVIPTP
HHHHCCCCCCC
>Mature Secondary Structure
MQALVIGSGGREHALAWKLLQSPQITRVYWAPGNGGAQQMPRCQSVALAATDIEGLVRFA
CCEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHCCCHHHHEEEHHHHHHHHHHH
TVQGIRLTVVGPELPLVEGIVDAFQAAGLPIFGPSRAAAQLEGSKLWAKQLMQEAGIPTA
EECCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCC
PAVGFADLSEALAHLNSRSYPVVIKKDGLAAGKGVTVAQNREEAVQALEAALAKPGDQVL
CCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCHHH
IEEFIPGQEVSVLALTDGQICVPLPPAQDYKRLGEGDTGPNTGGMGAHAPADHLVTPEGL
HHHCCCCCCEEEEEEECCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
ARIQTQILDPTLEALQRRGIRYQGVIYAGLMITPSGDPYVLEYNCRFGDPEAQVLLPLLE
HHHHHHHHCHHHHHHHHCCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCHHHEEHHHHH
NPLDEVLLACIEGRLAQIQLKWKPGYAACVVMAAPGYPDAYERGIPISGLDAAATTGALV
CCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCHHHHCCCCCCCCCHHHHCCEEE
FHAGTRQAGKQLLSDGGRVLCLTGQGATLSEALRQAYAAVEQVQFPKAYYRFDIGSHALG
EECCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCC
PAVRRGVIPTP
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]