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The map label for this gene is yugF [H]

Identifier: 86605207

GI number: 86605207

Start: 506500

End: 507435

Strand: Reverse

Name: yugF [H]

Synonym: CYA_0489

Alternate gene names: 86605207

Gene position: 507435-506500 (Counterclockwise)

Preceding gene: 86605210

Following gene: 86605203

Centisome position: 17.3

GC content: 62.61

Gene sequence:

>936_bases
ATGGCTTCAACATCGGCATCGGCCACTCCTATCTCGGCACGCTGGATTTGGCGGGGGCAATCCGTCCATTACGTCAAACA
GGGCGAGCAGGGGCAGCCTTTGCTTTTGGTGCACGGCTTTGGGGCTTCCACCGACCACTGGCGTAAAAACATTCCCGAAT
TGGCTCAACACTACCAGGTCTATGCCATCGATTTGCTCGGCTTTGGCCGTTCGGCCAAGCCCAACTGGGACTATCGCGCC
GAGATCTGGCGGGATCAACTGCGGGATTTCTGTCAACAGGTGATCCGGCGGCCTGTGGTGGCCATTGGCAACTCCCTGGG
CGGCTATGTGGTGCTCAGCCTGGCGGCAGAGTGGCCGGAGTGGGTGCGGGGGGTGGTGCTGCTTAACGGGGCAGGCGGCT
TTTCCACGGTTAAGGGATCCCCATCTGGCTGGCGGCAATGGCTGGGTGGCCTGGTAGGCTGGGGCCTGCGCCAGCGCCTG
GTGTCTTACCTATTGTTTCAGTATTTGCGGCAGCCGCGGGTGATTCGCTCCAAGCTGAAGCAGGTCTATTACGACCCGGC
GGCAGTGACGGATCAACTGGTGGAAGACATTCATCGCCCCACGCGGGATCCGGGGGCTGCCGATGTGTTCGTGGCCCTGA
TGCGGGGCGGGCAGAAGGGGCGCTATGTGGACGAGCTGCTGCGCAGCTTGGTGCGCCCCCTGTTGTTGATTTGGGGCGAA
CGGGATCCCTGGATGCGGGTCCGCGAGCGCTCTAAGCTCTACCGGGCTCACTACCCTCAGGCGGTGGAATACTTCCTGGA
GGCCGGCCACTGCCCTCACGATGAGCGCCCCGAAGAGGTCAACGCCCTGATCCATCGCTGGATCGAGCTGGGGATCCCGG
CCACCACCTCTCCTCCCCTTTCCAAAGCAGCGCCGGTCTCAGACCTGCATCCTTAG

Upstream 100 bases:

>100_bases
GTGTTTAGGATACGCTGCTCGGCTACAGCTCCAGAGTCGGCCGAGCAGAATCTAAAAGAATCTAGAATACTTAAATAACA
AAAGCTTACAAGCGACCTCT

Downstream 100 bases:

>100_bases
CCTTGGGAGCTAGGCGGGCTTGACTGCCAGCTCCGGCGGCGGATCCCCTTCCAGGCCGATGGCGTACTTCAGCCATTCCC
GATGGGATCCGCTGCCGACG

Product: alpha/beta fold family hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MASTSASATPISARWIWRGQSVHYVKQGEQGQPLLLVHGFGASTDHWRKNIPELAQHYQVYAIDLLGFGRSAKPNWDYRA
EIWRDQLRDFCQQVIRRPVVAIGNSLGGYVVLSLAAEWPEWVRGVVLLNGAGGFSTVKGSPSGWRQWLGGLVGWGLRQRL
VSYLLFQYLRQPRVIRSKLKQVYYDPAAVTDQLVEDIHRPTRDPGAADVFVALMRGGQKGRYVDELLRSLVRPLLLIWGE
RDPWMRVRERSKLYRAHYPQAVEYFLEAGHCPHDERPEEVNALIHRWIELGIPATTSPPLSKAAPVSDLHP

Sequences:

>Translated_311_residues
MASTSASATPISARWIWRGQSVHYVKQGEQGQPLLLVHGFGASTDHWRKNIPELAQHYQVYAIDLLGFGRSAKPNWDYRA
EIWRDQLRDFCQQVIRRPVVAIGNSLGGYVVLSLAAEWPEWVRGVVLLNGAGGFSTVKGSPSGWRQWLGGLVGWGLRQRL
VSYLLFQYLRQPRVIRSKLKQVYYDPAAVTDQLVEDIHRPTRDPGAADVFVALMRGGQKGRYVDELLRSLVRPLLLIWGE
RDPWMRVRERSKLYRAHYPQAVEYFLEAGHCPHDERPEEVNALIHRWIELGIPATTSPPLSKAAPVSDLHP
>Mature_310_residues
ASTSASATPISARWIWRGQSVHYVKQGEQGQPLLLVHGFGASTDHWRKNIPELAQHYQVYAIDLLGFGRSAKPNWDYRAE
IWRDQLRDFCQQVIRRPVVAIGNSLGGYVVLSLAAEWPEWVRGVVLLNGAGGFSTVKGSPSGWRQWLGGLVGWGLRQRLV
SYLLFQYLRQPRVIRSKLKQVYYDPAAVTDQLVEDIHRPTRDPGAADVFVALMRGGQKGRYVDELLRSLVRPLLLIWGER
DPWMRVRERSKLYRAHYPQAVEYFLEAGHCPHDERPEEVNALIHRWIELGIPATTSPPLSKAAPVSDLHP

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=282, Percent_Identity=25.886524822695, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558492, Length=101, Percent_Identity=34.6534653465347, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI32566936, Length=101, Percent_Identity=34.6534653465347, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25146278, Length=99, Percent_Identity=34.3434343434343, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586385, Length=282, Percent_Identity=24.113475177305, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 35357; Mature: 35226

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTSASATPISARWIWRGQSVHYVKQGEQGQPLLLVHGFGASTDHWRKNIPELAQHYQV
CCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
YAIDLLGFGRSAKPNWDYRAEIWRDQLRDFCQQVIRRPVVAIGNSLGGYVVLSLAAEWPE
HEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHH
WVRGVVLLNGAGGFSTVKGSPSGWRQWLGGLVGWGLRQRLVSYLLFQYLRQPRVIRSKLK
HHCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
QVYYDPAAVTDQLVEDIHRPTRDPGAADVFVALMRGGQKGRYVDELLRSLVRPLLLIWGE
HHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
RDPWMRVRERSKLYRAHYPQAVEYFLEAGHCPHDERPEEVNALIHRWIELGIPATTSPPL
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
SKAAPVSDLHP
CCCCCCCCCCC
>Mature Secondary Structure 
ASTSASATPISARWIWRGQSVHYVKQGEQGQPLLLVHGFGASTDHWRKNIPELAQHYQV
CCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
YAIDLLGFGRSAKPNWDYRAEIWRDQLRDFCQQVIRRPVVAIGNSLGGYVVLSLAAEWPE
HEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHH
WVRGVVLLNGAGGFSTVKGSPSGWRQWLGGLVGWGLRQRLVSYLLFQYLRQPRVIRSKLK
HHCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
QVYYDPAAVTDQLVEDIHRPTRDPGAADVFVALMRGGQKGRYVDELLRSLVRPLLLIWGE
HHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
RDPWMRVRERSKLYRAHYPQAVEYFLEAGHCPHDERPEEVNALIHRWIELGIPATTSPPL
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
SKAAPVSDLHP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]