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The map label for this gene is gatB

Identifier: 86605079

GI number: 86605079

Start: 376702

End: 378174

Strand: Reverse

Name: gatB

Synonym: CYA_0359

Alternate gene names: 86605079

Gene position: 378174-376702 (Counterclockwise)

Preceding gene: 86605080

Following gene: 86605077

Centisome position: 12.89

GC content: 60.96

Gene sequence:

>1473_bases
ATGACGGTTGCTGCCCCTGCCAAAGTGGAATACGAAGCTGTGATTGGGCTGGAGGTGCACTGCCAGCTCAGCACCCGCTC
CAAGATCTTCTCCAGCAGCGCGACGGCCTTTGGCGCTCCCCCCAACACCCAAATTGACCCTGTCTGCATGGGCCTGCCCG
GCACCCTGCCCGTGCTGAACGAGAAGGTGCTGGAATACGCGGTCAAGGCTGGCCTGGCCCTCAATTGCACCATCGCCCCC
TACAGCAAGTTCGACCGCAAGCAGTACTTCTACCCCGACCTGCCCAAAAACTACCAAATCTCCCAGTACGACCTGCCCAT
TGCCACCCACGGCTGGGTCGAGATCCAGCTCAGCGACGGCCGCAGCAAGCGCATCGGCATCACCCGTTTGCACATAGAAG
AAGACGCGGGCAAATTGGTGCATGCCGGCAGCGATCGGCTGTCGGGATCCAGCTATTCCCTGGTGGATTTCAACCGGGCA
GGAGTTCCCCTCATCGAGATCGTCAGCGAGCCGGATATCCGCACTGGGGAAGAAGCAGCCGAGTATGTACAGGAGCTGCG
CCGCATCCTCCGCTACGCCGGGCTGTGCGATGGCAACCTACAGGAGGGATCCCTGCGCTGCGACGTCAACATCTCGGTGC
GCCCGCTGGGCTCCCCGACCTTTGGCACGAAGGTGGAAATCAAGAACATGAACTCCTTCAACGCCATCCAGCGGGCCATC
GAGTACGAGTTCAACCGCCAAGTTAAGGCCGTGGAAGCAGGCGAGCCCATCGTACAGGAGACCCGCCTCTGGGAAGAAAA
CAGCCAGCGCACCATCAGCATGCGCAAGAAAGAGGGATCCAGCGACTACCGCTATTTTCCGGAGCCGGATCTGCCGCCCA
TTCGTGTGACGGAAGCGCAAAAAGCCCGCTGGCAGGCGGAGCTGCCGGAATTGCCCAGTGTGAAGCGGCGGCGCTACCAA
CAGGTGTACGGCCTCTCGGTGTATGACTCCCGCTACCTCAGCGACGAACGCAACACCGCCGAGTACTTTGAGGCCGTGGT
TGCTGCCGGCGCCGACCCGAAGGCAGCCGCCAACTGGATGATGAGCGACATCGCCAGCTACCTCAACACCCACAAGCTGG
ACTACCCGGACATCGCCTTGAAGCCGGAAACCCTGGCTGAGCTAATCGGCCTCATCGAGCAAGGCACTATTTCCAGCAAA
ATTGCCAAGGAGATCCTGCCGGAGCTGCTGGAAAAAGGCGGCTCGGCCCGCGCCCTGGTGGAGGCCAAGGGCCTGACCCA
GATCTCCGATAGCAGCCTCTTGGAGCCAATGATCGCCGAGGTGCTGGCCGAGAACCCCGAGCAACTGCAGCAGTACCGGG
AGGGCAAAACCAAGCTCTTCGGCTACTTTGTCGGGCAACTGATGAAAAAAACCCAAGGACGGGCGGATCCCAAGCTGGCC
AACGAGCTGCTCAAGCGGCATCTAGACGGCTAA

Upstream 100 bases:

>100_bases
GCCTCTCCACCTTCGAGATCCGCCCCACCGTTTGAGGCAGCAGCTCAGCAGCTAGCGATATCCCACTAGAATGGGAAAAC
CTACTCTTTCTCGTTTGGCC

Downstream 100 bases:

>100_bases
TTCCAGGCTTCGGTGAGGGAATAGCCCAATTCCTGGATCATGCGCCGCAGCAACGGCAAACTCAGCCCAATGACGTTGCC
AGGGCACCCTTCAATCCGCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTVAAPAKVEYEAVIGLEVHCQLSTRSKIFSSSATAFGAPPNTQIDPVCMGLPGTLPVLNEKVLEYAVKAGLALNCTIAP
YSKFDRKQYFYPDLPKNYQISQYDLPIATHGWVEIQLSDGRSKRIGITRLHIEEDAGKLVHAGSDRLSGSSYSLVDFNRA
GVPLIEIVSEPDIRTGEEAAEYVQELRRILRYAGLCDGNLQEGSLRCDVNISVRPLGSPTFGTKVEIKNMNSFNAIQRAI
EYEFNRQVKAVEAGEPIVQETRLWEENSQRTISMRKKEGSSDYRYFPEPDLPPIRVTEAQKARWQAELPELPSVKRRRYQ
QVYGLSVYDSRYLSDERNTAEYFEAVVAAGADPKAAANWMMSDIASYLNTHKLDYPDIALKPETLAELIGLIEQGTISSK
IAKEILPELLEKGGSARALVEAKGLTQISDSSLLEPMIAEVLAENPEQLQQYREGKTKLFGYFVGQLMKKTQGRADPKLA
NELLKRHLDG

Sequences:

>Translated_490_residues
MTVAAPAKVEYEAVIGLEVHCQLSTRSKIFSSSATAFGAPPNTQIDPVCMGLPGTLPVLNEKVLEYAVKAGLALNCTIAP
YSKFDRKQYFYPDLPKNYQISQYDLPIATHGWVEIQLSDGRSKRIGITRLHIEEDAGKLVHAGSDRLSGSSYSLVDFNRA
GVPLIEIVSEPDIRTGEEAAEYVQELRRILRYAGLCDGNLQEGSLRCDVNISVRPLGSPTFGTKVEIKNMNSFNAIQRAI
EYEFNRQVKAVEAGEPIVQETRLWEENSQRTISMRKKEGSSDYRYFPEPDLPPIRVTEAQKARWQAELPELPSVKRRRYQ
QVYGLSVYDSRYLSDERNTAEYFEAVVAAGADPKAAANWMMSDIASYLNTHKLDYPDIALKPETLAELIGLIEQGTISSK
IAKEILPELLEKGGSARALVEAKGLTQISDSSLLEPMIAEVLAENPEQLQQYREGKTKLFGYFVGQLMKKTQGRADPKLA
NELLKRHLDG
>Mature_489_residues
TVAAPAKVEYEAVIGLEVHCQLSTRSKIFSSSATAFGAPPNTQIDPVCMGLPGTLPVLNEKVLEYAVKAGLALNCTIAPY
SKFDRKQYFYPDLPKNYQISQYDLPIATHGWVEIQLSDGRSKRIGITRLHIEEDAGKLVHAGSDRLSGSSYSLVDFNRAG
VPLIEIVSEPDIRTGEEAAEYVQELRRILRYAGLCDGNLQEGSLRCDVNISVRPLGSPTFGTKVEIKNMNSFNAIQRAIE
YEFNRQVKAVEAGEPIVQETRLWEENSQRTISMRKKEGSSDYRYFPEPDLPPIRVTEAQKARWQAELPELPSVKRRRYQQ
VYGLSVYDSRYLSDERNTAEYFEAVVAAGADPKAAANWMMSDIASYLNTHKLDYPDIALKPETLAELIGLIEQGTISSKI
AKEILPELLEKGGSARALVEAKGLTQISDSSLLEPMIAEVLAENPEQLQQYREGKTKLFGYFVGQLMKKTQGRADPKLAN
ELLKRHLDG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=39.6, Blast_Score=335, Evalue=8e-92,
Organism=Caenorhabditis elegans, GI115534094, Length=309, Percent_Identity=39.8058252427184, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=529, Percent_Identity=31.1909262759924, Blast_Score=237, Evalue=4e-63,
Organism=Drosophila melanogaster, GI45550808, Length=499, Percent_Identity=36.6733466933868, Blast_Score=314, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_SYNJA (Q2JXB3)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_473842.1
- ProteinModelPortal:   Q2JXB3
- SMR:   Q2JXB3
- STRING:   Q2JXB3
- GeneID:   3897971
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_0359
- TIGR:   CYA_0359
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   SSP321327:CYA_0359-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54668; Mature: 54537

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAAPAKVEYEAVIGLEVHCQLSTRSKIFSSSATAFGAPPNTQIDPVCMGLPGTLPVLN
CCCCCCCCEEEEEEEEEEEEEEECHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHH
EKVLEYAVKAGLALNCTIAPYSKFDRKQYFYPDLPKNYQISQYDLPIATHGWVEIQLSDG
HHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCCCCCCEEECCCEEECCEEEEEECCC
RSKRIGITRLHIEEDAGKLVHAGSDRLSGSSYSLVDFNRAGVPLIEIVSEPDIRTGEEAA
CCCEEEEEEEEEECCCCCEEECCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHH
EYVQELRRILRYAGLCDGNLQEGSLRCDVNISVRPLGSPTFGTKVEIKNMNSFNAIQRAI
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEECCCHHHHHHHHH
EYEFNRQVKAVEAGEPIVQETRLWEENSQRTISMRKKEGSSDYRYFPEPDLPPIRVTEAQ
HHHHCCCEEEECCCCCHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCCCCCCEEECCHH
KARWQAELPELPSVKRRRYQQVYGLSVYDSRYLSDERNTAEYFEAVVAAGADPKAAANWM
HHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH
MSDIASYLNTHKLDYPDIALKPETLAELIGLIEQGTISSKIAKEILPELLEKGGSARALV
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEE
EAKGLTQISDSSLLEPMIAEVLAENPEQLQQYREGKTKLFGYFVGQLMKKTQGRADPKLA
EECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
NELLKRHLDG
HHHHHHHCCC
>Mature Secondary Structure 
TVAAPAKVEYEAVIGLEVHCQLSTRSKIFSSSATAFGAPPNTQIDPVCMGLPGTLPVLN
CCCCCCCEEEEEEEEEEEEEEECHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHH
EKVLEYAVKAGLALNCTIAPYSKFDRKQYFYPDLPKNYQISQYDLPIATHGWVEIQLSDG
HHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCCCCCCEEECCCEEECCEEEEEECCC
RSKRIGITRLHIEEDAGKLVHAGSDRLSGSSYSLVDFNRAGVPLIEIVSEPDIRTGEEAA
CCCEEEEEEEEEECCCCCEEECCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHH
EYVQELRRILRYAGLCDGNLQEGSLRCDVNISVRPLGSPTFGTKVEIKNMNSFNAIQRAI
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEECCCHHHHHHHHH
EYEFNRQVKAVEAGEPIVQETRLWEENSQRTISMRKKEGSSDYRYFPEPDLPPIRVTEAQ
HHHHCCCEEEECCCCCHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCCCCCCEEECCHH
KARWQAELPELPSVKRRRYQQVYGLSVYDSRYLSDERNTAEYFEAVVAAGADPKAAANWM
HHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH
MSDIASYLNTHKLDYPDIALKPETLAELIGLIEQGTISSKIAKEILPELLEKGGSARALV
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEE
EAKGLTQISDSSLLEPMIAEVLAENPEQLQQYREGKTKLFGYFVGQLMKKTQGRADPKLA
EECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
NELLKRHLDG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA