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The map label for this gene is lysS

Identifier: 86605053

GI number: 86605053

Start: 350698

End: 352224

Strand: Reverse

Name: lysS

Synonym: CYA_0333

Alternate gene names: 86605053

Gene position: 352224-350698 (Counterclockwise)

Preceding gene: 86605054

Following gene: 86605049

Centisome position: 12.01

GC content: 64.96

Gene sequence:

>1527_bases
ATGTCCACTCTCCCCAGCAGCAGCGAAGCTGCCCAACGGGCCGCCCGCCTGGAAAAGGCCCGCCACCTGCAGGAGCAAGG
GATCCTGCCCTACGCCTATTCTTTCCCGGTTACCCACTTGGCTGCCGAGTTGCAGGCGCAATTTGCCGATCTCGAGCCCG
GCGCCGAGCGGGAGGATGTGCAGGTGGCGGTGGCCGGGCGGATCCGGGCGCGGCGGGTCATGGGCAAACTGGCCTTCTTT
ACCCTGCAGGATCGCTCTGGCACCATCCAGCTCTACCTGGAAAAGCAACGGCTGACGCAGCACATGGGGGAGCTGGCCTT
CAAGAACCTGGACAAGCTGCTTGACGTGGGCGATTGGCTGGGGGCCAAGGGCTGTCTCAAGCGCACCGAGAAAGGGGAGC
TGTCGGTGCGGGTGGACGAGTACCAGGTGCTGACCAAGGCCCTGCTGCCCCTGCCCGATAAGTGGCACGGCCTACGGGAT
GTGGAGAAACGCTACCGCCAGCGCTACGTTGACCTCATCGTCAACCCCGAGGTCAAGCAAACCTTGCTCGACCGGGCCAA
GATCGTCCGCTCCCTGCGGCAGACCCTGGAGGAGCGGGGCTTTGTGGAGATCGAGACGCCGGTGCTGCAGGTGATCCCCG
GCGGGGCGGAGGCGCGTCCCTTCATCACCCACCACAACGCCCTGGATCTAGATCTCTACTTGCGCATTGCCACCGAGCTG
CACCTGAAGCGGCTGGTGGTGGGCGGGATCGAGCGGGTGTACGAAATTGGGCGAGTCTTTCGCAACGAGGGCATCTCCAC
CCGCCACAACCCGGAGTTTACCTCGCTGGAGGCCTACCAAGCCTTTGCCGACTACCACACGATGATGGAGCTGACCGAAG
CCCTGGTCTGCAACGCCGCCCTGGCAGTGCGGGGATCCCTGCAAATTACTTACCAAGGGGAGACCATTGACCTCACCCCA
CCCTGGCGGCGGGTAACGATCCAGGAGTTGGTGGAGGAGGCCACCGGCATCTTCATCGACGTGGAAAAGGGATCCACCGC
CGAGCTCTTGCCCGCCCTGGCCCAGCAGGGGATCCCCGATCTCTCCCCCCACGACTCCCCTGGCAAGCTGTTGGTCAAAG
CCTTCGAGCACTGCTGCGAGAGCCGCCTGCGCCAGCCGACCTTTGTTCTCGACTATCCCCTGGAGGTGTGTCCGCTCACC
AAGCCCCACCGCAGCAAGCCGGGGTTGGCGGAGCGCTTCGAGCTGTTTATCGTCGGGCGGGAGACGGCCAACGCCTACTC
GGAGCTGACCGACCCGGTGGATCAGCGGCAGCGGCTGGAGGCCCAGGCGGCCCTGAAGGCAGCCGGAGACGAAGAGGCCC
ACTTTTTGGACGAAGACTTCCTCACTGCCCTGGAGTACGGCCTTCCCCCCACCGGTGGCCTGGGCATCGGCATCGACCGT
CTGGCGATGCTGCTGTGCGACGCTGCCAGCATCCGCGATGTCATCGCCTTCCCCCTGCTGCGACCGGAGACGGGAGAAAG
CCCCTGA

Upstream 100 bases:

>100_bases
GAAAGACTTTTGGAGCTGCTGCGTTCCCACGGGATCGCGCCGCTGGAAGCGTAAAAAAGGATCCCGTCCGTCCCTGAAAT
CCTCTATCCCTTTTACCCCT

Downstream 100 bases:

>100_bases
ATAGGGGCCTAGGCCACAAGGGCATGGTTTAGGGGAGTTACATCATCCCCGCACCGCCGGAGCGGCTCGCTCCTGTTTTC
GTCTTGGGTTCGGGCTTTTC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MSTLPSSSEAAQRAARLEKARHLQEQGILPYAYSFPVTHLAAELQAQFADLEPGAEREDVQVAVAGRIRARRVMGKLAFF
TLQDRSGTIQLYLEKQRLTQHMGELAFKNLDKLLDVGDWLGAKGCLKRTEKGELSVRVDEYQVLTKALLPLPDKWHGLRD
VEKRYRQRYVDLIVNPEVKQTLLDRAKIVRSLRQTLEERGFVEIETPVLQVIPGGAEARPFITHHNALDLDLYLRIATEL
HLKRLVVGGIERVYEIGRVFRNEGISTRHNPEFTSLEAYQAFADYHTMMELTEALVCNAALAVRGSLQITYQGETIDLTP
PWRRVTIQELVEEATGIFIDVEKGSTAELLPALAQQGIPDLSPHDSPGKLLVKAFEHCCESRLRQPTFVLDYPLEVCPLT
KPHRSKPGLAERFELFIVGRETANAYSELTDPVDQRQRLEAQAALKAAGDEEAHFLDEDFLTALEYGLPPTGGLGIGIDR
LAMLLCDAASIRDVIAFPLLRPETGESP

Sequences:

>Translated_508_residues
MSTLPSSSEAAQRAARLEKARHLQEQGILPYAYSFPVTHLAAELQAQFADLEPGAEREDVQVAVAGRIRARRVMGKLAFF
TLQDRSGTIQLYLEKQRLTQHMGELAFKNLDKLLDVGDWLGAKGCLKRTEKGELSVRVDEYQVLTKALLPLPDKWHGLRD
VEKRYRQRYVDLIVNPEVKQTLLDRAKIVRSLRQTLEERGFVEIETPVLQVIPGGAEARPFITHHNALDLDLYLRIATEL
HLKRLVVGGIERVYEIGRVFRNEGISTRHNPEFTSLEAYQAFADYHTMMELTEALVCNAALAVRGSLQITYQGETIDLTP
PWRRVTIQELVEEATGIFIDVEKGSTAELLPALAQQGIPDLSPHDSPGKLLVKAFEHCCESRLRQPTFVLDYPLEVCPLT
KPHRSKPGLAERFELFIVGRETANAYSELTDPVDQRQRLEAQAALKAAGDEEAHFLDEDFLTALEYGLPPTGGLGIGIDR
LAMLLCDAASIRDVIAFPLLRPETGESP
>Mature_507_residues
STLPSSSEAAQRAARLEKARHLQEQGILPYAYSFPVTHLAAELQAQFADLEPGAEREDVQVAVAGRIRARRVMGKLAFFT
LQDRSGTIQLYLEKQRLTQHMGELAFKNLDKLLDVGDWLGAKGCLKRTEKGELSVRVDEYQVLTKALLPLPDKWHGLRDV
EKRYRQRYVDLIVNPEVKQTLLDRAKIVRSLRQTLEERGFVEIETPVLQVIPGGAEARPFITHHNALDLDLYLRIATELH
LKRLVVGGIERVYEIGRVFRNEGISTRHNPEFTSLEAYQAFADYHTMMELTEALVCNAALAVRGSLQITYQGETIDLTPP
WRRVTIQELVEEATGIFIDVEKGSTAELLPALAQQGIPDLSPHDSPGKLLVKAFEHCCESRLRQPTFVLDYPLEVCPLTK
PHRSKPGLAERFELFIVGRETANAYSELTDPVDQRQRLEAQAALKAAGDEEAHFLDEDFLTALEYGLPPTGGLGIGIDRL
AMLLCDAASIRDVIAFPLLRPETGESP

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=500, Percent_Identity=44.8, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI5031815, Length=500, Percent_Identity=44.8, Blast_Score=423, Evalue=1e-118,
Organism=Escherichia coli, GI1790571, Length=490, Percent_Identity=49.1836734693878, Blast_Score=470, Evalue=1e-133,
Organism=Escherichia coli, GI1789256, Length=495, Percent_Identity=49.6969696969697, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI87082379, Length=333, Percent_Identity=31.2312312312312, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1788173, Length=250, Percent_Identity=30, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=492, Percent_Identity=44.1056910569106, Blast_Score=403, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17535927, Length=492, Percent_Identity=44.1056910569106, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71994340, Length=504, Percent_Identity=43.6507936507937, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32566633, Length=269, Percent_Identity=26.0223048327138, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=465, Percent_Identity=22.3655913978495, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=525, Percent_Identity=40.5714285714286, Blast_Score=388, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324256, Length=480, Percent_Identity=33.125, Blast_Score=197, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6323011, Length=492, Percent_Identity=23.9837398373984, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325153, Length=248, Percent_Identity=25.4032258064516, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24640849, Length=490, Percent_Identity=45.1020408163265, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24640851, Length=487, Percent_Identity=44.35318275154, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24584738, Length=315, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56943; Mature: 56812

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLPSSSEAAQRAARLEKARHLQEQGILPYAYSFPVTHLAAELQAQFADLEPGAEREDV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHCCHHHHHHHHHHHHHCCCCCCCCCCE
QVAVAGRIRARRVMGKLAFFTLQDRSGTIQLYLEKQRLTQHMGELAFKNLDKLLDVGDWL
EEEEHHHHHHHHHHHHHHEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
GAKGCLKRTEKGELSVRVDEYQVLTKALLPLPDKWHGLRDVEKRYRQRYVDLIVNPEVKQ
CCHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
TLLDRAKIVRSLRQTLEERGFVEIETPVLQVIPGGAEARPFITHHNALDLDLYLRIATEL
HHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHH
HLKRLVVGGIERVYEIGRVFRNEGISTRHNPEFTSLEAYQAFADYHTMMELTEALVCNAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVRGSLQITYQGETIDLTPPWRRVTIQELVEEATGIFIDVEKGSTAELLPALAQQGIPD
HEEECEEEEEECCCEEECCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCC
LSPHDSPGKLLVKAFEHCCESRLRQPTFVLDYPLEVCPLTKPHRSKPGLAERFELFIVGR
CCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCCCHHHEEEEEEEEC
ETANAYSELTDPVDQRQRLEAQAALKAAGDEEAHFLDEDFLTALEYGLPPTGGLGIGIDR
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LAMLLCDAASIRDVIAFPLLRPETGESP
HHHHHHCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
STLPSSSEAAQRAARLEKARHLQEQGILPYAYSFPVTHLAAELQAQFADLEPGAEREDV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHCCHHHHHHHHHHHHHCCCCCCCCCCE
QVAVAGRIRARRVMGKLAFFTLQDRSGTIQLYLEKQRLTQHMGELAFKNLDKLLDVGDWL
EEEEHHHHHHHHHHHHHHEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
GAKGCLKRTEKGELSVRVDEYQVLTKALLPLPDKWHGLRDVEKRYRQRYVDLIVNPEVKQ
CCHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHH
TLLDRAKIVRSLRQTLEERGFVEIETPVLQVIPGGAEARPFITHHNALDLDLYLRIATEL
HHHHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHH
HLKRLVVGGIERVYEIGRVFRNEGISTRHNPEFTSLEAYQAFADYHTMMELTEALVCNAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVRGSLQITYQGETIDLTPPWRRVTIQELVEEATGIFIDVEKGSTAELLPALAQQGIPD
HEEECEEEEEECCCEEECCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCCCC
LSPHDSPGKLLVKAFEHCCESRLRQPTFVLDYPLEVCPLTKPHRSKPGLAERFELFIVGR
CCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCCCHHHEEEEEEEEC
ETANAYSELTDPVDQRQRLEAQAALKAAGDEEAHFLDEDFLTALEYGLPPTGGLGIGIDR
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LAMLLCDAASIRDVIAFPLLRPETGESP
HHHHHHCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA