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The map label for this gene is pgk

Identifier: 86605036

GI number: 86605036

Start: 323857

End: 325095

Strand: Reverse

Name: pgk

Synonym: CYA_0315

Alternate gene names: 86605036

Gene position: 325095-323857 (Counterclockwise)

Preceding gene: 86605040

Following gene: 86605029

Centisome position: 11.08

GC content: 63.6

Gene sequence:

>1239_bases
ATGGCCAAACAAACACTGGGATCCCTGCTGTCATCCGGCTTTGATCTCAAGGGCAAGCGTGTGCTGGTGCGGGCCGACTT
CAACGTCCCTCTGGATCCCCAGGGCAACATCACCGACGACACCCGCATTCGCGCCTCCCTGCCCACCATCCAAGCCTTGG
CCCAGGCGGGCGCCAAGGTCATCCTCACCAGCCACCTGGGCCGCCCCATCCAGAAAGACAAGGCGACCGGCGCCATCCAG
ATCGCCCGCGAGGGCAATAGCCTCGCCCCTGTGGCCGTCCGCCTTGCCCAGCTCCTGGGCCAGCCGGTGGCCTTTGCCCC
CGATTGCATTGGCCCTGAGGCCGAAGCCGTGGTCAGCTCTCTGGAAAACGGCCAGGTGGCCCTGCTGGAAAACGTCCGCT
TCCACCCCGAAGAGGAAGCCAACGATCCAGAATTTGCCCGCAAGCTGGCCTCCCTGGCCGACTTGTTTGTCAACGACGCC
TTTGGCAGCGCCCACCGCGCCCACGCCTCCACTGCCGGCGTCACCGCCTACCTCCAGCCCGCTGTGGCCGGCTACCTGGT
GGAAAAGGAGCTGCAATTTTTATCAGGCGCCATCGAGAACCCCCAGCGACCGCTAGCGGCCATCATCGGCGGCTCCAAAG
TCTCTACTAAGATTGGCGTCATCGAGCGCCTGCTGGAAAAAGTGGACAAGCTTCTGCTGGGCGGCGGCATGATCTTCACC
TTCTACCAAGCCCAAGGGATCCCAACCGGCAAGTCTTTGGTGGAAACCGACAAGCTGGATCTGGCCCGCTCCCTGATGGA
GAAGGCCAAAGCGCGGGGCGTGGAGCTGCTGCTGCCGGTGGACGTGGTGGTCGCCGATAGGTTTGACAAAGACGCCAACG
CCCAGACGGTTTCCATCCACGCCATTCCGGAAGACTGGATGGGCCTGGACATCGGCCCCGAATCGGTCAAAGCCTTCCAG
GCCGCCCTGCAAGGGTGCAAAACCGTAGTCTGGAACGGGCCGATGGGTGTGTTTGAGTTCGACCGCTTTGCCGCTGGCAC
AGAAGCCATTGCCCGCACCCTGGCCGACCTCACCCAGGCCGGTGCCATCACTATTATCGGCGGCGGCGACTCGGTGGCGG
CTGTGGAAAAAGTGGGCCTAGCCGACAAAATGACCCACATTTCCACCGGCGGCGGCGCCAGCCTGGAGCTGCTGGAAGGC
AAAGAGCTGCCCGGCATCGCCGCCCTTAGCGAAGCGTAG

Upstream 100 bases:

>100_bases
GATTTCCAGAGCCCGGGTTTCCCGCCGGGATCCGATCCCTGGTAGGATACGGGAGAAAAACTAGAAAAAAATTCTCTTGG
CTCCAGGGGGAGGGACGACG

Downstream 100 bases:

>100_bases
GGTAGCTCGGCTCATTAACCCAAAAGCAGCAAAAAGCAAAAAAGTAGGGGCTGAGTTCAGCCCCTCTTTTAATCTGCTCG
GCGCAGATGGCCGCAGCAAT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MAKQTLGSLLSSGFDLKGKRVLVRADFNVPLDPQGNITDDTRIRASLPTIQALAQAGAKVILTSHLGRPIQKDKATGAIQ
IAREGNSLAPVAVRLAQLLGQPVAFAPDCIGPEAEAVVSSLENGQVALLENVRFHPEEEANDPEFARKLASLADLFVNDA
FGSAHRAHASTAGVTAYLQPAVAGYLVEKELQFLSGAIENPQRPLAAIIGGSKVSTKIGVIERLLEKVDKLLLGGGMIFT
FYQAQGIPTGKSLVETDKLDLARSLMEKAKARGVELLLPVDVVVADRFDKDANAQTVSIHAIPEDWMGLDIGPESVKAFQ
AALQGCKTVVWNGPMGVFEFDRFAAGTEAIARTLADLTQAGAITIIGGGDSVAAVEKVGLADKMTHISTGGGASLELLEG
KELPGIAALSEA

Sequences:

>Translated_412_residues
MAKQTLGSLLSSGFDLKGKRVLVRADFNVPLDPQGNITDDTRIRASLPTIQALAQAGAKVILTSHLGRPIQKDKATGAIQ
IAREGNSLAPVAVRLAQLLGQPVAFAPDCIGPEAEAVVSSLENGQVALLENVRFHPEEEANDPEFARKLASLADLFVNDA
FGSAHRAHASTAGVTAYLQPAVAGYLVEKELQFLSGAIENPQRPLAAIIGGSKVSTKIGVIERLLEKVDKLLLGGGMIFT
FYQAQGIPTGKSLVETDKLDLARSLMEKAKARGVELLLPVDVVVADRFDKDANAQTVSIHAIPEDWMGLDIGPESVKAFQ
AALQGCKTVVWNGPMGVFEFDRFAAGTEAIARTLADLTQAGAITIIGGGDSVAAVEKVGLADKMTHISTGGGASLELLEG
KELPGIAALSEA
>Mature_411_residues
AKQTLGSLLSSGFDLKGKRVLVRADFNVPLDPQGNITDDTRIRASLPTIQALAQAGAKVILTSHLGRPIQKDKATGAIQI
AREGNSLAPVAVRLAQLLGQPVAFAPDCIGPEAEAVVSSLENGQVALLENVRFHPEEEANDPEFARKLASLADLFVNDAF
GSAHRAHASTAGVTAYLQPAVAGYLVEKELQFLSGAIENPQRPLAAIIGGSKVSTKIGVIERLLEKVDKLLLGGGMIFTF
YQAQGIPTGKSLVETDKLDLARSLMEKAKARGVELLLPVDVVVADRFDKDANAQTVSIHAIPEDWMGLDIGPESVKAFQA
ALQGCKTVVWNGPMGVFEFDRFAAGTEAIARTLADLTQAGAITIIGGGDSVAAVEKVGLADKMTHISTGGGASLELLEGK
ELPGIAALSEA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=419, Percent_Identity=45.8233890214797, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI31543397, Length=419, Percent_Identity=44.6300715990453, Blast_Score=346, Evalue=2e-95,
Organism=Escherichia coli, GI1789294, Length=400, Percent_Identity=44.25, Blast_Score=309, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17508823, Length=428, Percent_Identity=48.8317757009346, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6319857, Length=425, Percent_Identity=49.1764705882353, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17136394, Length=417, Percent_Identity=46.2829736211031, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=43.4052757793765, Blast_Score=323, Evalue=2e-88,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_SYNJA (Q2JXF0)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_473799.1
- ProteinModelPortal:   Q2JXF0
- SMR:   Q2JXF0
- STRING:   Q2JXF0
- GeneID:   3898060
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_0315
- TIGR:   CYA_0315
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   SSP321327:CYA_0315-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43225; Mature: 43094

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 42-42 BINDING 133-133 BINDING 166-166 BINDING 217-217 BINDING 308-308 BINDING 339-339

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQTLGSLLSSGFDLKGKRVLVRADFNVPLDPQGNITDDTRIRASLPTIQALAQAGAKV
CCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCEE
ILTSHLGRPIQKDKATGAIQIAREGNSLAPVAVRLAQLLGQPVAFAPDCIGPEAEAVVSS
EEEHHCCCCCHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LENGQVALLENVRFHPEEEANDPEFARKLASLADLFVNDAFGSAHRAHASTAGVTAYLQP
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHH
AVAGYLVEKELQFLSGAIENPQRPLAAIIGGSKVSTKIGVIERLLEKVDKLLLGGGMIFT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FYQAQGIPTGKSLVETDKLDLARSLMEKAKARGVELLLPVDVVVADRFDKDANAQTVSIH
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEECCCCCCCCCEEEEEE
AIPEDWMGLDIGPESVKAFQAALQGCKTVVWNGPMGVFEFDRFAAGTEAIARTLADLTQA
ECCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHC
GAITIIGGGDSVAAVEKVGLADKMTHISTGGGASLELLEGKELPGIAALSEA
CEEEEEECCCCCHHHHHHCCHHHHHEEECCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
AKQTLGSLLSSGFDLKGKRVLVRADFNVPLDPQGNITDDTRIRASLPTIQALAQAGAKV
CHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCEE
ILTSHLGRPIQKDKATGAIQIAREGNSLAPVAVRLAQLLGQPVAFAPDCIGPEAEAVVSS
EEEHHCCCCCHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
LENGQVALLENVRFHPEEEANDPEFARKLASLADLFVNDAFGSAHRAHASTAGVTAYLQP
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHH
AVAGYLVEKELQFLSGAIENPQRPLAAIIGGSKVSTKIGVIERLLEKVDKLLLGGGMIFT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
FYQAQGIPTGKSLVETDKLDLARSLMEKAKARGVELLLPVDVVVADRFDKDANAQTVSIH
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEECCCCCCCCCEEEEEE
AIPEDWMGLDIGPESVKAFQAALQGCKTVVWNGPMGVFEFDRFAAGTEAIARTLADLTQA
ECCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHC
GAITIIGGGDSVAAVEKVGLADKMTHISTGGGASLELLEGKELPGIAALSEA
CEEEEEECCCCCHHHHHHCCHHHHHEEECCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA