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The map label for this gene is phrB-1

Identifier: 86605025

GI number: 86605025

Start: 310546

End: 312012

Strand: Reverse

Name: phrB-1

Synonym: CYA_0303

Alternate gene names: 86605025

Gene position: 312012-310546 (Counterclockwise)

Preceding gene: 86605026

Following gene: 86605018

Centisome position: 10.64

GC content: 65.85

Gene sequence:

>1467_bases
ATGGCTGTTCTGCTTTGGTTTCGCACCGATTTGCGCCTGCTGGATCACCAGCCGCTGACGCGGGCTTGCCAGCAGGGATC
CTTCCTCATTCCCCTCTACTGCCTGGATCCGCGCCACTTTGGCCGGACGGCCTTTGGCTTTCCTAAGACCGGTCCTTTCC
GCGCCCAGTTCCTGCTGGAAAGCCTGGCTGACCTGCGGCAGCAGCTTCGGGCCCGCGGCTCTGATCTGGTCATCCGCCAA
GGCCAGCCGGAGCAGGTGATCCCGGCCCTGGCGCGGGAGTGGGGGGTGAAGGCTGTCTATGCCCACGAAGAGGTGGGCAC
GGAGGAGGAGGCGGTTGCCAGGGCCCTGCAGCAAGCGCTTCAGCCCTTAGGGATCCCGCTGCACCTGGAGTGGGGCCACA
CTCTCTACCATCCTGCTGACCTCCCCTTTGCCCTGGCAGACCTGCCGGAGGTGTTCACCCGCTTCCGCCAGCAGGTGGAA
GCCAAGGCCGCCATTCGCGCTCCCCTGCCCATCCCCTCCCTGCCGCCCCTGCCGCCAGGGCTGGACCCTGGCCCTCTGCC
CACTCTGGCGGAGCTGGGGTTGAGCTTGCCGCCGCCGGATCCCCGCGCCCGCTTTGTCTATGCCGGCGGCAGCACCGCCG
CCCAGGCCCGCCTGCAGACCTATATCTGGGATCTGGACCGGCTGCGCGTCTACAAAGAAACCCGCAACGGCATGCTGGAT
CCCAACGACAGCTCGCGTCTTTCTGCCTGGTTGGCGTTGGGTTGCCTTTCTCCTCGCACGGTGTATGCCGAAGTCAAGCG
CTACGAGGAGGAGCGGGTGCGCAACGAATCCACCTACTGGCTGGTCTTCGAGCTGCTGTGGCGGGATTACTTTCGCTTCA
TCCTGGCCAAGCATGGCGCCAAGCTGTTTTGCCCCGCAGGGATCCAGGGGATCCCCATCCCCTGGCAGCAGGACGGGGAT
CGCTTCCAGGCTTGGTGCCGCGGCGAGACCGGCTACCCCCTGGTGGACGCCAACATGCGGGAGTTGGCCGCCACCGGCTT
CATGTCCAACCGCGGGCGGCAGAATGTGGCCAGCTTCCTCACCAAAAACCTGGGGATCGACTGGCGTCTGGGGGCGGAAT
GGTTTGAATCGCTGCTGATTGACTACGACGTGGCCAGCAACTACGGCAACTGGAACTACACCGCCGGCGTGGGCAACGAT
GCCCGCGGCTTTCGCTACTTCCACATTCCCAAGCAATCCCGCGACTACGATCCCCAGGGCCACTACCTGCGCCACTGGCT
GCCGGAGCTGGCCGGGATCCCGGGGGACAAGATCCACGAGCCCTACCGCCTCAGCGCCGCTGAGCAAAAGCAGTTTGGCG
TGCGGCTGGGGGTAGACTATCCCCGCCCCGTGGTGGATTTGGGAAAGTCCCTGCAGGCCAGTCGGCGGGCTTACGAAGCG
GCCCTGGCCCGCTACCGCCGCGGCTAG

Upstream 100 bases:

>100_bases
ATCCCGGCTGCCCAAGTGCCCTATTTTGTCCCCAGCCCGGAGCTGGGACACCCTAGGAGAGAACGCCGAGGGGATCTGGA
AGCGCCGCCATCCGTCTAGA

Downstream 100 bases:

>100_bases
GGGGATCCCCTGGAAACGGGAGTAGCGGGCGGGGGGAGGAGGCGGCTAAGACGGCGCAGGCCAGGCAAACCTGCATCCAC
CACCACCACGCTGCCATCTG

Product: deoxyribodipyrimidine photolyase

Products: 2 pyrimidine residues (in DNA) [C]

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MAVLLWFRTDLRLLDHQPLTRACQQGSFLIPLYCLDPRHFGRTAFGFPKTGPFRAQFLLESLADLRQQLRARGSDLVIRQ
GQPEQVIPALAREWGVKAVYAHEEVGTEEEAVARALQQALQPLGIPLHLEWGHTLYHPADLPFALADLPEVFTRFRQQVE
AKAAIRAPLPIPSLPPLPPGLDPGPLPTLAELGLSLPPPDPRARFVYAGGSTAAQARLQTYIWDLDRLRVYKETRNGMLD
PNDSSRLSAWLALGCLSPRTVYAEVKRYEEERVRNESTYWLVFELLWRDYFRFILAKHGAKLFCPAGIQGIPIPWQQDGD
RFQAWCRGETGYPLVDANMRELAATGFMSNRGRQNVASFLTKNLGIDWRLGAEWFESLLIDYDVASNYGNWNYTAGVGND
ARGFRYFHIPKQSRDYDPQGHYLRHWLPELAGIPGDKIHEPYRLSAAEQKQFGVRLGVDYPRPVVDLGKSLQASRRAYEA
ALARYRRG

Sequences:

>Translated_488_residues
MAVLLWFRTDLRLLDHQPLTRACQQGSFLIPLYCLDPRHFGRTAFGFPKTGPFRAQFLLESLADLRQQLRARGSDLVIRQ
GQPEQVIPALAREWGVKAVYAHEEVGTEEEAVARALQQALQPLGIPLHLEWGHTLYHPADLPFALADLPEVFTRFRQQVE
AKAAIRAPLPIPSLPPLPPGLDPGPLPTLAELGLSLPPPDPRARFVYAGGSTAAQARLQTYIWDLDRLRVYKETRNGMLD
PNDSSRLSAWLALGCLSPRTVYAEVKRYEEERVRNESTYWLVFELLWRDYFRFILAKHGAKLFCPAGIQGIPIPWQQDGD
RFQAWCRGETGYPLVDANMRELAATGFMSNRGRQNVASFLTKNLGIDWRLGAEWFESLLIDYDVASNYGNWNYTAGVGND
ARGFRYFHIPKQSRDYDPQGHYLRHWLPELAGIPGDKIHEPYRLSAAEQKQFGVRLGVDYPRPVVDLGKSLQASRRAYEA
ALARYRRG
>Mature_487_residues
AVLLWFRTDLRLLDHQPLTRACQQGSFLIPLYCLDPRHFGRTAFGFPKTGPFRAQFLLESLADLRQQLRARGSDLVIRQG
QPEQVIPALAREWGVKAVYAHEEVGTEEEAVARALQQALQPLGIPLHLEWGHTLYHPADLPFALADLPEVFTRFRQQVEA
KAAIRAPLPIPSLPPLPPGLDPGPLPTLAELGLSLPPPDPRARFVYAGGSTAAQARLQTYIWDLDRLRVYKETRNGMLDP
NDSSRLSAWLALGCLSPRTVYAEVKRYEEERVRNESTYWLVFELLWRDYFRFILAKHGAKLFCPAGIQGIPIPWQQDGDR
FQAWCRGETGYPLVDANMRELAATGFMSNRGRQNVASFLTKNLGIDWRLGAEWFESLLIDYDVASNYGNWNYTAGVGNDA
RGFRYFHIPKQSRDYDPQGHYLRHWLPELAGIPGDKIHEPYRLSAAEQKQFGVRLGVDYPRPVVDLGKSLQASRRAYEAA
LARYRRG

Specific function: May have a photoreceptor function. Binds DNA; probably functions as a transcriptional repressor [H]

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=503, Percent_Identity=29.6222664015905, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI4758072, Length=508, Percent_Identity=28.9370078740157, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI188536103, Length=455, Percent_Identity=29.8901098901099, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1786926, Length=491, Percent_Identity=30.3462321792261, Blast_Score=182, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324962, Length=253, Percent_Identity=33.596837944664, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17137248, Length=491, Percent_Identity=29.3279022403259, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24585455, Length=491, Percent_Identity=29.3279022403259, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24648152, Length=529, Percent_Identity=27.0321361058601, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR014133
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: 4.1.99.3 [C]

Molecular weight: Translated: 55430; Mature: 55299

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLLWFRTDLRLLDHQPLTRACQQGSFLIPLYCLDPRHFGRTAFGFPKTGPFRAQFLLE
CEEEEEEECCHHHHCCCHHHHHHHCCCEEEEEEEECCHHCCCCCCCCCCCCCHHHHHHHH
SLADLRQQLRARGSDLVIRQGQPEQVIPALAREWGVKAVYAHEEVGTEEEAVARALQQAL
HHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCEEEEECHHCCCHHHHHHHHHHHHH
QPLGIPLHLEWGHTLYHPADLPFALADLPEVFTRFRQQVEAKAAIRAPLPIPSLPPLPPG
HHCCCEEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
LDPGPLPTLAELGLSLPPPDPRARFVYAGGSTAAQARLQTYIWDLDRLRVYKETRNGMLD
CCCCCCCHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PNDSSRLSAWLALGCLSPRTVYAEVKRYEEERVRNESTYWLVFELLWRDYFRFILAKHGA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
KLFCPAGIQGIPIPWQQDGDRFQAWCRGETGYPLVDANMRELAATGFMSNRGRQNVASFL
CEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCHHHHHHHH
TKNLGIDWRLGAEWFESLLIDYDVASNYGNWNYTAGVGNDARGFRYFHIPKQSRDYDPQG
HHHCCCCEEECHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCC
HYLRHWLPELAGIPGDKIHEPYRLSAAEQKQFGVRLGVDYPRPVVDLGKSLQASRRAYEA
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHH
ALARYRRG
HHHHHCCC
>Mature Secondary Structure 
AVLLWFRTDLRLLDHQPLTRACQQGSFLIPLYCLDPRHFGRTAFGFPKTGPFRAQFLLE
EEEEEEECCHHHHCCCHHHHHHHCCCEEEEEEEECCHHCCCCCCCCCCCCCHHHHHHHH
SLADLRQQLRARGSDLVIRQGQPEQVIPALAREWGVKAVYAHEEVGTEEEAVARALQQAL
HHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCEEEEECHHCCCHHHHHHHHHHHHH
QPLGIPLHLEWGHTLYHPADLPFALADLPEVFTRFRQQVEAKAAIRAPLPIPSLPPLPPG
HHCCCEEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
LDPGPLPTLAELGLSLPPPDPRARFVYAGGSTAAQARLQTYIWDLDRLRVYKETRNGMLD
CCCCCCCHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PNDSSRLSAWLALGCLSPRTVYAEVKRYEEERVRNESTYWLVFELLWRDYFRFILAKHGA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
KLFCPAGIQGIPIPWQQDGDRFQAWCRGETGYPLVDANMRELAATGFMSNRGRQNVASFL
CEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCHHHHHHHH
TKNLGIDWRLGAEWFESLLIDYDVASNYGNWNYTAGVGNDARGFRYFHIPKQSRDYDPQG
HHHCCCCEEECHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCC
HYLRHWLPELAGIPGDKIHEPYRLSAAEQKQFGVRLGVDYPRPVVDLGKSLQASRRAYEA
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHH
ALARYRRG
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; FADH2; Flavin; pterin [C]

Metal ions: NA

Kcat value (1/min): 2.4 [C]

Specific activity: NA

Km value (mM): NA

Substrates: cyclobutadipyrimidine (in DNA) [C]

Specific reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) [C]

General reaction: C-C-bond cleavage [C]

Inhibitor: yeast DNA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]