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The map label for this gene is carA

Identifier: 86604920

GI number: 86604920

Start: 195697

End: 196884

Strand: Reverse

Name: carA

Synonym: CYA_0195

Alternate gene names: 86604920

Gene position: 196884-195697 (Counterclockwise)

Preceding gene: 86604921

Following gene: 86604915

Centisome position: 6.71

GC content: 66.08

Gene sequence:

>1188_bases
ATGGTCTCCTCAACTCTTGAAGCTCCGGAAAAAGTCTTCCCCTGGCACGCTCGGCAGCCGGCTCTGTTGGTGCTGGCCGA
TGGCACCGCCTTCCCCGGCTGGTCGTTTGGGGCGCCAGGCACGGCGGTGGGGGAGGTGGTGTTCAACACGGGCATGACCG
GCTACCAGGAGGTGATTACCGATCCAAGCTATCGGGGGCAGTTGATCACGTTCACCTGCCCGGAGTTGGGGAACACCGGG
ATCAACGAGCTGGATCAGGAGTCGGCCCGGCCCCAGGCCGCCGGGATCATCGCCCGCAACGTGTCGCGCCTGGCCAGCTC
CTGGCGGGCCACAGGAACCCTGCCTGAATATCTAAAAGGGCACGGGATCCCGGGCATTGCTGGGGTGGACACGCGGGCCC
TGACGCGGCGGCTGCGCTCCCAGGGGGTGATGAATGGGGCCATCTCGACGGAGATCCTGGATCCGCAGGCGTTGCTGGAG
CGGGTGCGCCAGGCCCCTTCCATGCAGGGGCTGAGCCTGGTGGCAGAGGTAACAACCCCTAAGCCCTACGAGTGGCTGGA
GCCCACCCCCGCCGACTGGGACTATGGCCGCAGCCAAGGGATCCCGATTCCGGATCCCCCCTTGCGGGTGGTGGCGCTGG
ATTTTGGCATCAAGCGCAACATCTTGCGCCGCCTGGCCCGCTACGGCTGCCGGGTGATGGTGTTGCCGGCCCACGCCAGC
CCCGAGGAGATTTTGAGTTACAACCCCGACGGGATCCTCCTCTCCAACGGGCCGGGGGATCCGGCGGCAGAGACGACGGC
CATCCGCACCACCCAGGCGCTGCTGCAGAGCGGGAAGCCGATGTTCGGCATCTGCCTAGGCCACCAGATCCTCAGCCTGG
CCCTGGGCGGATCCACCTACAAGCTCAAGTTCGGCCACCGCGGCCTCAACCACCCCTGTGGGCTGGAGAAGGAAGTGGAG
ATCACCAGCCAAAACCACGGCTTTGCGGTGGAGGCCGCTTCGCTCCCTGGGGATGGGGTGGCGATCAGCTACCTCAACCT
CAATGACCGCACCGTGGCCGGGATCCGCCACCGCCAGTTGCCCCTCTTTTCGGTGCAGTACCACCCGGAAGCCAGCCCCG
GCCCCCACGATGCCGACCACCTCTTTCGGGAGTTTGTCGAGCTGATGCTGCAAAACCGCTCGCGTTAG

Upstream 100 bases:

>100_bases
CTAGTTGGGGTAGGATAGGCAGGCTGCCGATCCCGGTCCATTGCCCTCACCCCCAGCCCCTCTCCCTTTGGGAGAGGGGG
GTTGTCTAGGTTCACAGGTT

Downstream 100 bases:

>100_bases
CCGCCCCTCACCCCCAGCCCCTCTCCCAAAGGGAGAGGGGAGCAGAGGCGTAGGGCGAGGGCGGCACGGGCTTCTTCTCG
GTCGTCGAAGTGGATTTTCT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MVSSTLEAPEKVFPWHARQPALLVLADGTAFPGWSFGAPGTAVGEVVFNTGMTGYQEVITDPSYRGQLITFTCPELGNTG
INELDQESARPQAAGIIARNVSRLASSWRATGTLPEYLKGHGIPGIAGVDTRALTRRLRSQGVMNGAISTEILDPQALLE
RVRQAPSMQGLSLVAEVTTPKPYEWLEPTPADWDYGRSQGIPIPDPPLRVVALDFGIKRNILRRLARYGCRVMVLPAHAS
PEEILSYNPDGILLSNGPGDPAAETTAIRTTQALLQSGKPMFGICLGHQILSLALGGSTYKLKFGHRGLNHPCGLEKEVE
ITSQNHGFAVEAASLPGDGVAISYLNLNDRTVAGIRHRQLPLFSVQYHPEASPGPHDADHLFREFVELMLQNRSR

Sequences:

>Translated_395_residues
MVSSTLEAPEKVFPWHARQPALLVLADGTAFPGWSFGAPGTAVGEVVFNTGMTGYQEVITDPSYRGQLITFTCPELGNTG
INELDQESARPQAAGIIARNVSRLASSWRATGTLPEYLKGHGIPGIAGVDTRALTRRLRSQGVMNGAISTEILDPQALLE
RVRQAPSMQGLSLVAEVTTPKPYEWLEPTPADWDYGRSQGIPIPDPPLRVVALDFGIKRNILRRLARYGCRVMVLPAHAS
PEEILSYNPDGILLSNGPGDPAAETTAIRTTQALLQSGKPMFGICLGHQILSLALGGSTYKLKFGHRGLNHPCGLEKEVE
ITSQNHGFAVEAASLPGDGVAISYLNLNDRTVAGIRHRQLPLFSVQYHPEASPGPHDADHLFREFVELMLQNRSR
>Mature_395_residues
MVSSTLEAPEKVFPWHARQPALLVLADGTAFPGWSFGAPGTAVGEVVFNTGMTGYQEVITDPSYRGQLITFTCPELGNTG
INELDQESARPQAAGIIARNVSRLASSWRATGTLPEYLKGHGIPGIAGVDTRALTRRLRSQGVMNGAISTEILDPQALLE
RVRQAPSMQGLSLVAEVTTPKPYEWLEPTPADWDYGRSQGIPIPDPPLRVVALDFGIKRNILRRLARYGCRVMVLPAHAS
PEEILSYNPDGILLSNGPGDPAAETTAIRTTQALLQSGKPMFGICLGHQILSLALGGSTYKLKFGHRGLNHPCGLEKEVE
ITSQNHGFAVEAASLPGDGVAISYLNLNDRTVAGIRHRQLPLFSVQYHPEASPGPHDADHLFREFVELMLQNRSR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=42.2976501305483, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI21361331, Length=390, Percent_Identity=37.1794871794872, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI169790915, Length=390, Percent_Identity=37.1794871794872, Blast_Score=238, Evalue=5e-63,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=50.3937007874016, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI193204318, Length=389, Percent_Identity=38.8174807197943, Blast_Score=243, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322331, Length=391, Percent_Identity=38.8746803069054, Blast_Score=249, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6324878, Length=387, Percent_Identity=38.2428940568475, Blast_Score=239, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6322638, Length=166, Percent_Identity=30.7228915662651, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45555749, Length=398, Percent_Identity=39.6984924623116, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24642586, Length=398, Percent_Identity=39.6984924623116, Blast_Score=244, Evalue=6e-65,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42804; Mature: 42804

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSTLEAPEKVFPWHARQPALLVLADGTAFPGWSFGAPGTAVGEVVFNTGMTGYQEVIT
CCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHC
DPSYRGQLITFTCPELGNTGINELDQESARPQAAGIIARNVSRLASSWRATGTLPEYLKG
CCCCCCEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
HGIPGIAGVDTRALTRRLRSQGVMNGAISTEILDPQALLERVRQAPSMQGLSLVAEVTTP
CCCCCCCCCCHHHHHHHHHHCCCCCCCCHHEECCHHHHHHHHHHCCCCCCCEEEEEECCC
KPYEWLEPTPADWDYGRSQGIPIPDPPLRVVALDFGIKRNILRRLARYGCRVMVLPAHAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCC
PEEILSYNPDGILLSNGPGDPAAETTAIRTTQALLQSGKPMFGICLGHQILSLALGGSTY
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEE
KLKFGHRGLNHPCGLEKEVEITSQNHGFAVEAASLPGDGVAISYLNLNDRTVAGIRHRQL
EEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCEEECCHHCCC
PLFSVQYHPEASPGPHDADHLFREFVELMLQNRSR
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVSSTLEAPEKVFPWHARQPALLVLADGTAFPGWSFGAPGTAVGEVVFNTGMTGYQEVIT
CCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHC
DPSYRGQLITFTCPELGNTGINELDQESARPQAAGIIARNVSRLASSWRATGTLPEYLKG
CCCCCCEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
HGIPGIAGVDTRALTRRLRSQGVMNGAISTEILDPQALLERVRQAPSMQGLSLVAEVTTP
CCCCCCCCCCHHHHHHHHHHCCCCCCCCHHEECCHHHHHHHHHHCCCCCCCEEEEEECCC
KPYEWLEPTPADWDYGRSQGIPIPDPPLRVVALDFGIKRNILRRLARYGCRVMVLPAHAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCC
PEEILSYNPDGILLSNGPGDPAAETTAIRTTQALLQSGKPMFGICLGHQILSLALGGSTY
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEE
KLKFGHRGLNHPCGLEKEVEITSQNHGFAVEAASLPGDGVAISYLNLNDRTVAGIRHRQL
EEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEEEECCCCEEECCHHCCC
PLFSVQYHPEASPGPHDADHLFREFVELMLQNRSR
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]