Click here to switch to the map view.

The map label for this gene is glcF [H]

Identifier: 86604862

GI number: 86604862

Start: 126728

End: 128053

Strand: Reverse

Name: glcF [H]

Synonym: CYA_0131

Alternate gene names: 86604862

Gene position: 128053-126728 (Counterclockwise)

Preceding gene: 86604864

Following gene: 86604858

Centisome position: 4.37

GC content: 64.33

Gene sequence:

>1326_bases
ATGAGCTCATCCAAAAGGCCGAGTTTTGACAGCCTGGATCCGCCGGATCCAAAGCTAATCGATGCCTGTGTGCACTGTGG
GTTTTGTCTGACCACCTGCCCCAGCTACCGCGTCCTCGGCAAGGAGACAGACTCGCCGCGGGGGCGCATCTACCAGATGG
ACGCCCTCAATCAAGGCCAAATCTCTCTCTCGGCAGCAGTGGTTTCCCATTTCGATACCTGCCTGGGCTGTCTGGCCTGC
GTTACCGCCTGTCCTTCGGGGGTGCGCTACGACCGGCTGATCGCCGCCACCCGCCCGCAGATCGAGCGCAACCACCGCCG
CAACCTTTGGGATTGGCTGTACCGCCAGTTCATCTTCTGGCTCTTCCCCTACCCGGCGCGGCTGCGGCCTCTGCTCTGGT
CCCTTTGGCTCTACCAAAAAATGGGGGTCGGCGCCTGGATTCGCCAAGGGCTGGCGAAGGTCTCCCCGCGCCTGGCGGCC
ATGGAATCCCTCTTGCCCCCTCTTGAGGTGGGGCAACTGCTGCCCAAGCGCTACCCGGAGCGGATCCCGGCGGTGGGCGA
ACGCCGCTTTCGCGTGGGGCTGCTCCTCGGCTGCGTGCAGCGCCTGTTTGCCCCGCAGGTGAATGAGGCCAGCATTCGCG
TCTTGACCCACAACGGCTGTGAGGTGGTGATCCCGCCCGCCCAGGGCTGTTGTGCGGCCTTGCCCCACCACCAAGGGCAA
CTGCAGCAGGCAGAAGCCCTGGCCCGCCAGATGGTGGATGCTTTTGCCGGCTACGAGTTGGATGCCATTTTGGTGACTGC
CTCCGGCTGCGGCCACACCCTCAAGGAGTACGGCCACCTTTTGGCCAGGGATCCCGACTACCAAGAGAAAGGGGCTCAGT
TTGCGGCCAAAGTCAAGGATATCCAGGAGTTTCTGGCGCAGGTAGGGCTGACCGCTCCCCTGTACCCCCTGCAGCCGGAT
CCCTTGGTGCTGGCCTATCAGGATGCCTGCCACATGCTGCACGGGCAGCGCCTCAGCCAGGAGCCGCGACAACTGCTGCG
GCAAATTCCCGGGCTGGAGCTGCGGGATCCTGTCGATGCCGCCCTCTGCTGCGGCAGCTCCGGCGTCTACAACCTGCTGC
AGCCCGAGGTGGCCGACGAGCTGGGATCCCAGAAGGTGGCCAACCTGCTCAACACCGGCGCCCAGGGGATCGTCTCCGCC
AATATCGGCTGCCGCCTGCAGCTCCAGAAGCACCTGCGTCTGCAAGGCAAGGCCGGGATCCCCATTTATCACCCGATGGA
ACTCCTGGATCGCGCTCTCCGACCGCCCACTCCTCCTTCTAGCTAG

Upstream 100 bases:

>100_bases
AAAAAACCCCGGAAGCAAAAGGAGGGGACAGGATCCCCGAACGGCCAATTCTCTCTTTACGGGGGTGATGAAGTGTTGAA
AGCGCGATAGGTCGAGGCCG

Downstream 100 bases:

>100_bases
AGCTTGAGGGCGGCACGGGGATCATAGGACTGAACCCGCTGCAGCTTTTCGTAGAGCTCCCGCTCCTCCTCCGAAACACG
GGTGGGCAAATAGATCTGAA

Product: glycolate oxidase, iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSSSKRPSFDSLDPPDPKLIDACVHCGFCLTTCPSYRVLGKETDSPRGRIYQMDALNQGQISLSAAVVSHFDTCLGCLAC
VTACPSGVRYDRLIAATRPQIERNHRRNLWDWLYRQFIFWLFPYPARLRPLLWSLWLYQKMGVGAWIRQGLAKVSPRLAA
MESLLPPLEVGQLLPKRYPERIPAVGERRFRVGLLLGCVQRLFAPQVNEASIRVLTHNGCEVVIPPAQGCCAALPHHQGQ
LQQAEALARQMVDAFAGYELDAILVTASGCGHTLKEYGHLLARDPDYQEKGAQFAAKVKDIQEFLAQVGLTAPLYPLQPD
PLVLAYQDACHMLHGQRLSQEPRQLLRQIPGLELRDPVDAALCCGSSGVYNLLQPEVADELGSQKVANLLNTGAQGIVSA
NIGCRLQLQKHLRLQGKAGIPIYHPMELLDRALRPPTPPSS

Sequences:

>Translated_441_residues
MSSSKRPSFDSLDPPDPKLIDACVHCGFCLTTCPSYRVLGKETDSPRGRIYQMDALNQGQISLSAAVVSHFDTCLGCLAC
VTACPSGVRYDRLIAATRPQIERNHRRNLWDWLYRQFIFWLFPYPARLRPLLWSLWLYQKMGVGAWIRQGLAKVSPRLAA
MESLLPPLEVGQLLPKRYPERIPAVGERRFRVGLLLGCVQRLFAPQVNEASIRVLTHNGCEVVIPPAQGCCAALPHHQGQ
LQQAEALARQMVDAFAGYELDAILVTASGCGHTLKEYGHLLARDPDYQEKGAQFAAKVKDIQEFLAQVGLTAPLYPLQPD
PLVLAYQDACHMLHGQRLSQEPRQLLRQIPGLELRDPVDAALCCGSSGVYNLLQPEVADELGSQKVANLLNTGAQGIVSA
NIGCRLQLQKHLRLQGKAGIPIYHPMELLDRALRPPTPPSS
>Mature_440_residues
SSSKRPSFDSLDPPDPKLIDACVHCGFCLTTCPSYRVLGKETDSPRGRIYQMDALNQGQISLSAAVVSHFDTCLGCLACV
TACPSGVRYDRLIAATRPQIERNHRRNLWDWLYRQFIFWLFPYPARLRPLLWSLWLYQKMGVGAWIRQGLAKVSPRLAAM
ESLLPPLEVGQLLPKRYPERIPAVGERRFRVGLLLGCVQRLFAPQVNEASIRVLTHNGCEVVIPPAQGCCAALPHHQGQL
QQAEALARQMVDAFAGYELDAILVTASGCGHTLKEYGHLLARDPDYQEKGAQFAAKVKDIQEFLAQVGLTAPLYPLQPDP
LVLAYQDACHMLHGQRLSQEPRQLLRQIPGLELRDPVDAALCCGSSGVYNLLQPEVADELGSQKVANLLNTGAQGIVSAN
IGCRLQLQKHLRLQGKAGIPIYHPMELLDRALRPPTPPSS

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=421, Percent_Identity=35.3919239904988, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1786497, Length=241, Percent_Identity=24.896265560166, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 48814; Mature: 48683

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSKRPSFDSLDPPDPKLIDACVHCGFCLTTCPSYRVLGKETDSPRGRIYQMDALNQGQ
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEEECCCCCCC
ISLSAAVVSHFDTCLGCLACVTACPSGVRYDRLIAATRPQIERNHRRNLWDWLYRQFIFW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHH
LFPYPARLRPLLWSLWLYQKMGVGAWIRQGLAKVSPRLAAMESLLPPLEVGQLLPKRYPE
HCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCHH
RIPAVGERRFRVGLLLGCVQRLFAPQVNEASIRVLTHNGCEVVIPPAQGCCAALPHHQGQ
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEECCCCCHHHHCCCCCCH
LQQAEALARQMVDAFAGYELDAILVTASGCGHTLKEYGHLLARDPDYQEKGAQFAAKVKD
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IQEFLAQVGLTAPLYPLQPDPLVLAYQDACHMLHGQRLSQEPRQLLRQIPGLELRDPVDA
HHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHH
ALCCGSSGVYNLLQPEVADELGSQKVANLLNTGAQGIVSANIGCRLQLQKHLRLQGKAGI
HHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHCCCCCCC
PIYHPMELLDRALRPPTPPSS
CCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSSKRPSFDSLDPPDPKLIDACVHCGFCLTTCPSYRVLGKETDSPRGRIYQMDALNQGQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEEEECCCCCCC
ISLSAAVVSHFDTCLGCLACVTACPSGVRYDRLIAATRPQIERNHRRNLWDWLYRQFIFW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHH
LFPYPARLRPLLWSLWLYQKMGVGAWIRQGLAKVSPRLAAMESLLPPLEVGQLLPKRYPE
HCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCHH
RIPAVGERRFRVGLLLGCVQRLFAPQVNEASIRVLTHNGCEVVIPPAQGCCAALPHHQGQ
HCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEECCCCCHHHHCCCCCCH
LQQAEALARQMVDAFAGYELDAILVTASGCGHTLKEYGHLLARDPDYQEKGAQFAAKVKD
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IQEFLAQVGLTAPLYPLQPDPLVLAYQDACHMLHGQRLSQEPRQLLRQIPGLELRDPVDA
HHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHH
ALCCGSSGVYNLLQPEVADELGSQKVANLLNTGAQGIVSANIGCRLQLQKHLRLQGKAGI
HHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHCCCCCCC
PIYHPMELLDRALRPPTPPSS
CCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]