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The map label for this gene is thiC

Identifier: 86604744

GI number: 86604744

Start: 11810

End: 13177

Strand: Reverse

Name: thiC

Synonym: CYA_0011

Alternate gene names: 86604744

Gene position: 13177-11810 (Counterclockwise)

Preceding gene: 86604745

Following gene: 86604743

Centisome position: 0.45

GC content: 56.65

Gene sequence:

>1368_bases
ATGCGCAGCGAATGGGTTGCCCGCCGTCGTGGGCAAGCAAATGTTACGCAAATGCACTTTGCCCGGCAGGGCGTGATTAC
CGAAGAGATGGACTACGTGGCTCGGCGAGAGAACCTACCGCCGGAGCTGATCCGCTCAGAAGTGGCGCGGGGCCGCATGA
TCATCCCTGCCAATATCAATCACACCAACCTAGAGCCCATGTGCATCGGGATCGCCTCCCGCTGCAAAGTCAATGCTAAT
ATTGGCGCCTCCCCCAGCTCCTCTGGATTAGCCGAAGAGCTGGAAAAGCTGAAATTGGCCATTAAATACGGCGCCGATAC
CGTTATGGATCTGTCCACCGGCGGCGGCGACTTGGACGAAATTCGCACGGCTATTATCCAAGCTTCTCCGGTGCCCATCG
GCACGGTGCCCATCTACCAAGCTCTAGAAAGTGTCCATGGCAATGTCGAAAAACTCTCGGCAGAAGATATCTTGCACATC
ATCGAAAAGCAGGCCCAGCAAGGGGTGGACTACATGACCATCCATGCCGGGATCCTCATCGAATATCTGCCATTGGTGCG
CAACCGTCTCACAGGAATTGTCTCTCGCGGAGGTGGGATCCTGGCCCGTTGGATGTTGGCCCACCACAAGCAAAACCCGC
TCTACACCCACTTCCGCGACATCATCGAGATCTTCAAGAAATACGATGTTTCTTTTAGCCTGGGCGATGCTTTGCGGCCC
GGATGTCTGCACGATGCTTCTGACGAGGCCCAGATGGCGGAGTTGAAAACCCTGGGGCAGTTGACCCGCATGGCCTGGGA
GCACGATGTGCAGGTGATGGTGGAAGGGCCAGGGCACGTGCCGATGGATCAAATTGAGTTTAACGTGCGCAAGCAAATGG
AGGAATGCGACGAGGCTCCTTTCTACGTTTTGGGGCCGCTGGTAACCGATATTGCCGCTGGCTACGACCACATCAGCTCT
GCCATTGGGGCGGCCTTGGCCGGCTGGTACGGGGCGGCGATGCTCTGCTACGTAACTCCCAAAGAACACCTGGGCTTGCC
CAATGCCGAGGATGTGCGCAACGGCCTCATTGCCTACAAAATTGCCGCCCACGCTGCCGATATTGCCCGCCACCGTCCTG
GGGCCAGGGATCGAGATGACGAGATGTCCAGAGCCCGCTACAACTTTGATTGGAACCGACAATTTGAGTTGTCTTTAGAC
CCAGAGCGGGCGCGAGAATATCACGACGAGACCTTGCCGGCAGATATCTACAAAACGGCGGAGTTTTGCTCGATGTGTGG
ACCGAAGTTCTGCCCCATGCAGACCAAAATGGACGCAGAAGCGTTGAGCGAGCTGGAGCGATTCTTGGCCAAGCAGCCAA
CAGCTTAG

Upstream 100 bases:

>100_bases
CGCTAGGAGTGTCTGGGGGCTGCCCCCAGGCTGAGATTACATCCTCAGAACCTGATCTGGGTCATACCAGCGTAGGAAAG
CGAGTACCGGAGTTGACACC

Downstream 100 bases:

>100_bases
GCATTAGCTTGCCCTGGGCCTCGAACGAAGTTGGAATCGACCTTGGTTCCATCCCTGCTCTCGGGGGAGTGGGGAGGGGC
TCTCTCTGCAAGCTATGCTG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MRSEWVARRRGQANVTQMHFARQGVITEEMDYVARRENLPPELIRSEVARGRMIIPANINHTNLEPMCIGIASRCKVNAN
IGASPSSSGLAEELEKLKLAIKYGADTVMDLSTGGGDLDEIRTAIIQASPVPIGTVPIYQALESVHGNVEKLSAEDILHI
IEKQAQQGVDYMTIHAGILIEYLPLVRNRLTGIVSRGGGILARWMLAHHKQNPLYTHFRDIIEIFKKYDVSFSLGDALRP
GCLHDASDEAQMAELKTLGQLTRMAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECDEAPFYVLGPLVTDIAAGYDHISS
AIGAALAGWYGAAMLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIARHRPGARDRDDEMSRARYNFDWNRQFELSLD
PERAREYHDETLPADIYKTAEFCSMCGPKFCPMQTKMDAEALSELERFLAKQPTA

Sequences:

>Translated_455_residues
MRSEWVARRRGQANVTQMHFARQGVITEEMDYVARRENLPPELIRSEVARGRMIIPANINHTNLEPMCIGIASRCKVNAN
IGASPSSSGLAEELEKLKLAIKYGADTVMDLSTGGGDLDEIRTAIIQASPVPIGTVPIYQALESVHGNVEKLSAEDILHI
IEKQAQQGVDYMTIHAGILIEYLPLVRNRLTGIVSRGGGILARWMLAHHKQNPLYTHFRDIIEIFKKYDVSFSLGDALRP
GCLHDASDEAQMAELKTLGQLTRMAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECDEAPFYVLGPLVTDIAAGYDHISS
AIGAALAGWYGAAMLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIARHRPGARDRDDEMSRARYNFDWNRQFELSLD
PERAREYHDETLPADIYKTAEFCSMCGPKFCPMQTKMDAEALSELERFLAKQPTA
>Mature_455_residues
MRSEWVARRRGQANVTQMHFARQGVITEEMDYVARRENLPPELIRSEVARGRMIIPANINHTNLEPMCIGIASRCKVNAN
IGASPSSSGLAEELEKLKLAIKYGADTVMDLSTGGGDLDEIRTAIIQASPVPIGTVPIYQALESVHGNVEKLSAEDILHI
IEKQAQQGVDYMTIHAGILIEYLPLVRNRLTGIVSRGGGILARWMLAHHKQNPLYTHFRDIIEIFKKYDVSFSLGDALRP
GCLHDASDEAQMAELKTLGQLTRMAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECDEAPFYVLGPLVTDIAAGYDHISS
AIGAALAGWYGAAMLCYVTPKEHLGLPNAEDVRNGLIAYKIAAHAADIARHRPGARDRDDEMSRARYNFDWNRQFELSLD
PERAREYHDETLPADIYKTAEFCSMCGPKFCPMQTKMDAEALSELERFLAKQPTA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=458, Percent_Identity=58.7336244541485, Blast_Score=549, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_SYNJA (Q2JY68)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_473507.1
- STRING:   Q2JY68
- GeneID:   3897097
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_0011
- TIGR:   CYA_0011
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   EPMAIGI
- ProtClustDB:   PRK13352
- BioCyc:   SSP321327:CYA_0011-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 50739; Mature: 50739

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: BINDING 80-80 BINDING 109-109 BINDING 139-139 BINDING 175-175 BINDING 275-275 BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSEWVARRRGQANVTQMHFARQGVITEEMDYVARRENLPPELIRSEVARGRMIIPANIN
CCCHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HTNLEPMCIGIASRCKVNANIGASPSSSGLAEELEKLKLAIKYGADTVMDLSTGGGDLDE
CCCCCHHEEEHHHHCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
IRTAIIQASPVPIGTVPIYQALESVHGNVEKLSAEDILHIIEKQAQQGVDYMTIHAGILI
HHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
EYLPLVRNRLTGIVSRGGGILARWMLAHHKQNPLYTHFRDIIEIFKKYDVSFSLGDALRP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCC
GCLHDASDEAQMAELKTLGQLTRMAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECDEAP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDIAAGYDHISSAIGAALAGWYGAAMLCYVTPKEHLGLPNAEDVRNGLIAYK
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCHHHHH
IAAHAADIARHRPGARDRDDEMSRARYNFDWNRQFELSLDPERAREYHDETLPADIYKTA
HHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCHHHHHHH
EFCSMCGPKFCPMQTKMDAEALSELERFLAKQPTA
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRSEWVARRRGQANVTQMHFARQGVITEEMDYVARRENLPPELIRSEVARGRMIIPANIN
CCCHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HTNLEPMCIGIASRCKVNANIGASPSSSGLAEELEKLKLAIKYGADTVMDLSTGGGDLDE
CCCCCHHEEEHHHHCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHH
IRTAIIQASPVPIGTVPIYQALESVHGNVEKLSAEDILHIIEKQAQQGVDYMTIHAGILI
HHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH
EYLPLVRNRLTGIVSRGGGILARWMLAHHKQNPLYTHFRDIIEIFKKYDVSFSLGDALRP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCC
GCLHDASDEAQMAELKTLGQLTRMAWEHDVQVMVEGPGHVPMDQIEFNVRKQMEECDEAP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDIAAGYDHISSAIGAALAGWYGAAMLCYVTPKEHLGLPNAEDVRNGLIAYK
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHCCHHHHH
IAAHAADIARHRPGARDRDDEMSRARYNFDWNRQFELSLDPERAREYHDETLPADIYKTA
HHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCHHHHHHH
EFCSMCGPKFCPMQTKMDAEALSELERFLAKQPTA
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA