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The map label for this gene is hemNf

Identifier: 86361248

GI number: 86361248

Start: 584941

End: 586293

Strand: Reverse

Name: hemNf

Synonym: RHE_PF00519

Alternate gene names: 86361248

Gene position: 586293-584941 (Counterclockwise)

Preceding gene: 86361253

Following gene: 86361242

Centisome position: 91.25

GC content: 62.45

Gene sequence:

>1353_bases
ATGTCCAACGATCTGATCGCCAAATATGGCGATGCGCGGCTTCCGCGTTATACGAGTTACCCGACGGCGCCGGCTTTTTC
CGCTGCCGTGGGGCCGAATGAATATGCCAGCGCCCTCGCCGATATCGCCAAAGCCGGCCCGGTTTCGGTTTACCTCCACG
TCCCGTTCTGCCGCTCGATGTGCTGGTACTGCGGCTGCCACACGACCATCACCCGGCAAGATGCCCCGGTCGCCGACTAT
CTTGACGTGATGAGCCAAGAGATCGAGCTCGTCTCCTTTGCGGCTGGAAACGACGTGCCCGTCAAGAACGTGCACTTCGG
CGGCGGCACGCCGACGATCATGAGACCGCAGGAATTTTCCGCTCTGATGGCAAAGCTCAGGAGCGCCTTCGCTTTCGAGG
CGGACGCCGGCGTCGCCGTCGAGATCGATCCGCGCACGTTGGCTGCCCCCATGATCGAGGCGCTCGCCGAAAACGGCGTC
GACCGGGCAAGCCTCGGCGTCCAGAGTTTCGATCCGGTCGTGCAGGCAGCCATCAATCGGCTGCAGTCCTTCGAGCAGAC
GGAAACGGCGGTCGTCGGCCTGCGCTCTTCAGGCGTGAGCAGCATCAATTTCGACCTGATTTATGGCCTTCCGAAACAGA
CCGTCCAATCCTGCATCGAAACGGTCCGGCTGGCTGCGGAGCTGCGTCCCGAACGTTTCGCCGTTTTCGGTTATGCCCAC
ATACCCGCTTTCAAGAAGCATCAGCGCCTGATCGACGAAAGCACGTTGCCGGACGCAAAACAGCGCAACGAGCAGGCGGA
AGCGATCGCCGAGGAGCTGCAGAAGGTCGGATATCAGCGCATCGGGCTCGATCATTTTGCACTGCCGGACGATCAGCTGG
CGATCGCCGCGCGCAAGGGCGCGATGAGAAGGAACTTCCAGGGCTATACCACGGATGATTGCGACAACCTGATCGGCCTC
GGCGCATCTGCGATCGGGCGGCTGCCGACCGGTTATATGCAGAACCACGTGCCGCTCGGCCTCTATGCCGAGCGGATTGC
CTCCGGCGTGCTGCCGACCGCCAAGGGATATCTTCTCACAGAAGAGGACAAACTTCGGGCGAAGATCATCGAGAGGCTGA
TGTGCGATTTCGAAGCCGATCTCGGCAAGCTGAGCAGCGGGTCGGGTTTCGACAGCGGGTTCCTTGTCGAGCGCAACGAG
CGTCTCGGCGAACTCGTGGCGGACGGCGTCGTGACGATCAGCGGCGAGCGGATCGCGGTGTTCCAGGAGGCGCGCTTCAT
GGTCCGTGCCGTCGCGGCGGCGTTCGACGCCTATTTCGGCTCGCACGGGCGCACGCACAGCAAGGCAGCCTGA

Upstream 100 bases:

>100_bases
CCGGATGATTCTCGGCGAAGACCTCATCATTGATTTGGGACAAGGAAGACCGCGCGGCCGGACGTCATAAAGCATCCTGC
TTTTGAAGAGGGATGTGACA

Downstream 100 bases:

>100_bases
TGCATATCGCCGGAATGTGTGCAGCGTTCCGGGATAACCACACGTATAAGAACAAAGCTAAAACGCGTTGTGAAAACAAA
GAACATCAAGCCAGGTGGGA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSNDLIAKYGDARLPRYTSYPTAPAFSAAVGPNEYASALADIAKAGPVSVYLHVPFCRSMCWYCGCHTTITRQDAPVADY
LDVMSQEIELVSFAAGNDVPVKNVHFGGGTPTIMRPQEFSALMAKLRSAFAFEADAGVAVEIDPRTLAAPMIEALAENGV
DRASLGVQSFDPVVQAAINRLQSFEQTETAVVGLRSSGVSSINFDLIYGLPKQTVQSCIETVRLAAELRPERFAVFGYAH
IPAFKKHQRLIDESTLPDAKQRNEQAEAIAEELQKVGYQRIGLDHFALPDDQLAIAARKGAMRRNFQGYTTDDCDNLIGL
GASAIGRLPTGYMQNHVPLGLYAERIASGVLPTAKGYLLTEEDKLRAKIIERLMCDFEADLGKLSSGSGFDSGFLVERNE
RLGELVADGVVTISGERIAVFQEARFMVRAVAAAFDAYFGSHGRTHSKAA

Sequences:

>Translated_450_residues
MSNDLIAKYGDARLPRYTSYPTAPAFSAAVGPNEYASALADIAKAGPVSVYLHVPFCRSMCWYCGCHTTITRQDAPVADY
LDVMSQEIELVSFAAGNDVPVKNVHFGGGTPTIMRPQEFSALMAKLRSAFAFEADAGVAVEIDPRTLAAPMIEALAENGV
DRASLGVQSFDPVVQAAINRLQSFEQTETAVVGLRSSGVSSINFDLIYGLPKQTVQSCIETVRLAAELRPERFAVFGYAH
IPAFKKHQRLIDESTLPDAKQRNEQAEAIAEELQKVGYQRIGLDHFALPDDQLAIAARKGAMRRNFQGYTTDDCDNLIGL
GASAIGRLPTGYMQNHVPLGLYAERIASGVLPTAKGYLLTEEDKLRAKIIERLMCDFEADLGKLSSGSGFDSGFLVERNE
RLGELVADGVVTISGERIAVFQEARFMVRAVAAAFDAYFGSHGRTHSKAA
>Mature_449_residues
SNDLIAKYGDARLPRYTSYPTAPAFSAAVGPNEYASALADIAKAGPVSVYLHVPFCRSMCWYCGCHTTITRQDAPVADYL
DVMSQEIELVSFAAGNDVPVKNVHFGGGTPTIMRPQEFSALMAKLRSAFAFEADAGVAVEIDPRTLAAPMIEALAENGVD
RASLGVQSFDPVVQAAINRLQSFEQTETAVVGLRSSGVSSINFDLIYGLPKQTVQSCIETVRLAAELRPERFAVFGYAHI
PAFKKHQRLIDESTLPDAKQRNEQAEAIAEELQKVGYQRIGLDHFALPDDQLAIAARKGAMRRNFQGYTTDDCDNLIGLG
ASAIGRLPTGYMQNHVPLGLYAERIASGVLPTAKGYLLTEEDKLRAKIIERLMCDFEADLGKLSSGSGFDSGFLVERNER
LGELVADGVVTISGERIAVFQEARFMVRAVAAAFDAYFGSHGRTHSKAA

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=298, Percent_Identity=23.8255033557047, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI87082341, Length=445, Percent_Identity=38.2022471910112, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1789325, Length=317, Percent_Identity=26.1829652996845, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 48801; Mature: 48670

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDLIAKYGDARLPRYTSYPTAPAFSAAVGPNEYASALADIAKAGPVSVYLHVPFCRSM
CCCCCEECCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCEEEEEECHHHHHH
CWYCGCHTTITRQDAPVADYLDVMSQEIELVSFAAGNDVPVKNVHFGGGTPTIMRPQEFS
HHHHCCCCEECCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCCCCCCCHHHH
ALMAKLRSAFAFEADAGVAVEIDPRTLAAPMIEALAENGVDRASLGVQSFDPVVQAAINR
HHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHH
LQSFEQTETAVVGLRSSGVSSINFDLIYGLPKQTVQSCIETVRLAAELRPERFAVFGYAH
HHHHHHHHHHHEEECCCCCCEECEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHH
IPAFKKHQRLIDESTLPDAKQRNEQAEAIAEELQKVGYQRIGLDHFALPDDQLAIAARKG
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHH
AMRRNFQGYTTDDCDNLIGLGASAIGRLPTGYMQNHVPLGLYAERIASGVLPTAKGYLLT
HHHHCCCCCCCCCHHHHHHCCHHHHHCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCEEC
EEDKLRAKIIERLMCDFEADLGKLSSGSGFDSGFLVERNERLGELVADGVVTISGERIAV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHCCEEEECCCEEEH
FQEARFMVRAVAAAFDAYFGSHGRTHSKAA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SNDLIAKYGDARLPRYTSYPTAPAFSAAVGPNEYASALADIAKAGPVSVYLHVPFCRSM
CCCCEECCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCEEEEEECHHHHHH
CWYCGCHTTITRQDAPVADYLDVMSQEIELVSFAAGNDVPVKNVHFGGGTPTIMRPQEFS
HHHHCCCCEECCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCCCCCCCHHHH
ALMAKLRSAFAFEADAGVAVEIDPRTLAAPMIEALAENGVDRASLGVQSFDPVVQAAINR
HHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHH
LQSFEQTETAVVGLRSSGVSSINFDLIYGLPKQTVQSCIETVRLAAELRPERFAVFGYAH
HHHHHHHHHHHEEECCCCCCEECEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHH
IPAFKKHQRLIDESTLPDAKQRNEQAEAIAEELQKVGYQRIGLDHFALPDDQLAIAARKG
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHH
AMRRNFQGYTTDDCDNLIGLGASAIGRLPTGYMQNHVPLGLYAERIASGVLPTAKGYLLT
HHHHCCCCCCCCCHHHHHHCCHHHHHCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCCEEC
EEDKLRAKIIERLMCDFEADLGKLSSGSGFDSGFLVERNERLGELVADGVVTISGERIAV
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHCCEEEECCCEEEH
FQEARFMVRAVAAAFDAYFGSHGRTHSKAA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157943; 12597275 [H]