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The map label for this gene is nrtB

Identifier: 86361183

GI number: 86361183

Start: 510032

End: 510925

Strand: Direct

Name: nrtB

Synonym: RHE_PF00453

Alternate gene names: 86361183

Gene position: 510032-510925 (Clockwise)

Preceding gene: 86361182

Following gene: 86361184

Centisome position: 79.38

GC content: 63.2

Gene sequence:

>894_bases
ATGTCCGCCCTGGCAAATAAAGAAACTCCCTCGATCGTCGCCTCCGCCAAGCCGGCGGCGAAGGTTCTGTCATTCTCCGG
CAAGCAAGGATCGCGGATCGATTTTCGCCGTGCGGCGCTGACCGCACTCCAAAATGTCGTTCCGCCGGCCGTCGTGCTGG
CGCTCGTCCTCCTCCTCTGGCAGGCGCTCTGTTCATCGGCGGACGCCTCCCTGCCGTCGCCGCACCAGGTCTGGCAGGAC
AGCTACGATCTCATCGCCTACCCGTTCTTCAACTACGGCTCCCAGGATATCGGGCTCGGCTGGCGCGTGCTGGTTTCGCT
GCAGCGCGTCGCCTATGGCTTCGGTCTTGCCGCCGTCACAGGCGTCATCGTCGGGGCGATCATCGGCCAGTCGGTCTGGG
CGATGCGCGGCCTCGATCCGATCTTCCAGGTGCTGCGCACGGTGCCGCCGCTCGCCTGGCTACCGCTGTCGCTCGCCGCC
TTCCAAGATTCCAACCCTTCGGCGATCTTCGTTATATTCATCACCTCGATCTGGCCTGTCATCATCAATACCGCCGTCGG
CGTCCGCAATATTCCGCAGGATTATCGCAACGTCGCCGAGGTGCTGCGGCTGAACCAGTTCGAATTCTTCTGGAAGATCA
TGCTGCCGTCGGCAGCCCCTTACATCTTCACCGGCCTCAGGATCGGCGTCGGCCTTTCCTGGCTCGCCATCGTCGCGGCT
GAAATGCTGACCGGCGGTGTCGGTATCGGCTTCTTCATCTGGGACGCGTGGAACTCCTCGCGCCTTCCCGACATCATCGT
CGCGCTCGCCTATATCGGCATCGTCGGCTTTGCCCTCGACAAGCTGGTGGCCGCGCTCGGCAAACTCGTCACCCGCGGCG
CCATGGCCAACTAA

Upstream 100 bases:

>100_bases
CCTCCGCCTATCTCGACAGCCTTTCAATCAAGGCCGTCTCCTGATCCCGCCTCCGGCGCGCGCGGCGCGCCGGCCTTCCA
TTCCCGTGAGGAGCGCGCTG

Downstream 100 bases:

>100_bases
GGACCATTCTGATGAACGCCTATCTCAAGCTCGATCATATCGACAAGCATTTCGATCGCGGCGGCGTGCGCGCCGAAGTC
CTGAAGGATATCAACCTGAC

Product: nitrate ABC transporter, permease protein

Products: taurine [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSALANKETPSIVASAKPAAKVLSFSGKQGSRIDFRRAALTALQNVVPPAVVLALVLLLWQALCSSADASLPSPHQVWQD
SYDLIAYPFFNYGSQDIGLGWRVLVSLQRVAYGFGLAAVTGVIVGAIIGQSVWAMRGLDPIFQVLRTVPPLAWLPLSLAA
FQDSNPSAIFVIFITSIWPVIINTAVGVRNIPQDYRNVAEVLRLNQFEFFWKIMLPSAAPYIFTGLRIGVGLSWLAIVAA
EMLTGGVGIGFFIWDAWNSSRLPDIIVALAYIGIVGFALDKLVAALGKLVTRGAMAN

Sequences:

>Translated_297_residues
MSALANKETPSIVASAKPAAKVLSFSGKQGSRIDFRRAALTALQNVVPPAVVLALVLLLWQALCSSADASLPSPHQVWQD
SYDLIAYPFFNYGSQDIGLGWRVLVSLQRVAYGFGLAAVTGVIVGAIIGQSVWAMRGLDPIFQVLRTVPPLAWLPLSLAA
FQDSNPSAIFVIFITSIWPVIINTAVGVRNIPQDYRNVAEVLRLNQFEFFWKIMLPSAAPYIFTGLRIGVGLSWLAIVAA
EMLTGGVGIGFFIWDAWNSSRLPDIIVALAYIGIVGFALDKLVAALGKLVTRGAMAN
>Mature_296_residues
SALANKETPSIVASAKPAAKVLSFSGKQGSRIDFRRAALTALQNVVPPAVVLALVLLLWQALCSSADASLPSPHQVWQDS
YDLIAYPFFNYGSQDIGLGWRVLVSLQRVAYGFGLAAVTGVIVGAIIGQSVWAMRGLDPIFQVLRTVPPLAWLPLSLAAF
QDSNPSAIFVIFITSIWPVIINTAVGVRNIPQDYRNVAEVLRLNQFEFFWKIMLPSAAPYIFTGLRIGVGLSWLAIVAAE
MLTGGVGIGFFIWDAWNSSRLPDIIVALAYIGIVGFALDKLVAALGKLVTRGAMAN

Specific function: Probably part of a high-affinity binding-protein- dependent transport system for nitrate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786564, Length=208, Percent_Identity=35.0961538461538, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87081802, Length=160, Percent_Identity=31.875, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005889 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31987; Mature: 31856

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALANKETPSIVASAKPAAKVLSFSGKQGSRIDFRRAALTALQNVVPPAVVLALVLLLW
CCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QALCSSADASLPSPHQVWQDSYDLIAYPFFNYGSQDIGLGWRVLVSLQRVAYGFGLAAVT
HHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVIVGAIIGQSVWAMRGLDPIFQVLRTVPPLAWLPLSLAAFQDSNPSAIFVIFITSIWPV
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
IINTAVGVRNIPQDYRNVAEVLRLNQFEFFWKIMLPSAAPYIFTGLRIGVGLSWLAIVAA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EMLTGGVGIGFFIWDAWNSSRLPDIIVALAYIGIVGFALDKLVAALGKLVTRGAMAN
HHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SALANKETPSIVASAKPAAKVLSFSGKQGSRIDFRRAALTALQNVVPPAVVLALVLLLW
CCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QALCSSADASLPSPHQVWQDSYDLIAYPFFNYGSQDIGLGWRVLVSLQRVAYGFGLAAVT
HHHHCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVIVGAIIGQSVWAMRGLDPIFQVLRTVPPLAWLPLSLAAFQDSNPSAIFVIFITSIWPV
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
IINTAVGVRNIPQDYRNVAEVLRLNQFEFFWKIMLPSAAPYIFTGLRIGVGLSWLAIVAA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EMLTGGVGIGFFIWDAWNSSRLPDIIVALAYIGIVGFALDKLVAALGKLVTRGAMAN
HHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: taurine [Periplasm]; ATP; H2O [C]

Specific reaction: taurine [Periplasm] + ATP + H2O = taurine [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]