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The map label for this gene is ipdC [H]

Identifier: 86361179

GI number: 86361179

Start: 502380

End: 504035

Strand: Direct

Name: ipdC [H]

Synonym: RHE_PF00449

Alternate gene names: 86361179

Gene position: 502380-504035 (Clockwise)

Preceding gene: 86361178

Following gene: 86361182

Centisome position: 78.19

GC content: 54.17

Gene sequence:

>1656_bases
ATGGGCGATACATACACGGTTGGACAATATCTGGTCGACAGACTTCGCGAACTGGGTCTGGGACATTTGTTTTCGGTGGC
GGGCGATTATTCGATCGAATGGGTAAACAGCTATGTCGAGAAAAGCGATATTCAGGTCATCGAAGAGGTGAATGAACTGA
ATGCCGGTTATGCGGCCGACGGCTATGCGAGACTGAAAGGAATTGGCGCGCTGTGCGTCACCTATTCCGCAGGCTCGCTT
TGTGCGACAAATGCGATCGCCGGATGCTACGTCGAGAAAGTGCCGGTCGTCCTGATCAACGGTGCGCCAAGTGTCCAGAA
AACGCTGAGATTCGAACAAACCGGCTATAGTTCGCACCACTTCATCAGCGGGCGCGAAACGGATCGGCAGGTGTTCGAAT
ACATAACCGCCGCTACCGTTCGCATAGACAGCCCTCATCTTGCGCCAATGCTGATAGATTATGCGCTGACACAGTGCATC
ACGGAAAGACGTCCGGTCTATGTCGAGTTGCTGGAGGATATGGTCGACCTGGAATGCACGCGCCCGTCGAATGCATTGAA
AGCGGCGCCTGACATATCCGACGAGGACAGTCTCAATCAGTCGATCGCGCAAATCAGCGAAAGATTGCAGAACGCGACCA
AACCTCTGATCTGGATCGGTGTCGAGATCGACCGGTTCGGTCTTCACGACCAGGCGGAGCGGCTTATCCAGGACCTCAAA
ATTCCCTACGTGACCGAGCTTTTGAGCAAGGCCATCCTGTCGGAAGACGATGTCCAGTTTGCCGGGGTGTTCGATGGCCA
ATCGTCGTCATCCTATGTCCAGTCTTTGGTCAAAGACTCCGACTTCGTATTGGCGCTGGGCGTCTGGTTGACTGATATCA
ACGATTTGGGCTGGCCTATCGATCTCGACAAAACCGCATTCGCCTCATGGGATACAGTGAAATATGGCACGATCTTCAAC
GCACAGGTTTCGTTGGCGGATCTCGTCAACGGCTTGATCGACAAAAGGCTGACATGCAAACCCCAAAGCCTACCGGCGAA
AACGGCCCGACAGGCGCCGATCGTGAATCCGACAGGCGAAATCACCTATCAGGGCTTCTACGATTTCATTCAGCAGCAGA
TCGACGGAAACACCATCGTTGGTGCCGACGCCAGTTTGAATTATTTCGGGAGCCTGCTTCTTGAAGTGGGGGCTCGCCGC
GGCTTCATCGTCCAATCATCCTATTCGGCGATCGGTTATATTGGCCCGGCCGCGACTGGAGTTTCGCTGGCGAAGCAAGC
TAACCAGAGGCTGCTGGTCTTTGCCGGGGATGGCGGGTTTCAGATGACCGCTCAATGCCTCTCGACACAAACCCGTTTCA
ATCTCAACCCGATCATCTTCGTGATGGACAACGGCATCTATGGCGTCGAGCAATGGCTTGCCGATGCATCGGTTTTCCAT
GGTAACAGGCCGTTCTACACCTCATGCATCCTGCACCGATGGAATTACAGCAAGTTGGCAGAGGTCTTTGGCTGCCGGGG
CTGGAAAGTGGACACCTATGGCGAACTGGAGGATGCCATAAGCGGCGCCAAAGAAAACCTGAACAGTCCGTCCATCATCC
AGGTCGTGGTGCCTCAGCGGTCAATCCCCAATAATGCGAACTGGAAAGCAAGCTAG

Upstream 100 bases:

>100_bases
AATGCCCCGGAATATTCGACATATACATACCGCGCTTATTCGATAACGCGAGCGAGTTCTAATATTCGTCAATTCTCGTC
AACAGGGGAGCCGCTGGAAC

Downstream 100 bases:

>100_bases
AGCGGTTCTGGCGACGCGCACCAGGGCAGGCGATGAACTGCCGTCGCTCGATCGCTTCGACGTTATCGACCGGCCCCAAC
AATTTCTGCCCGCTCGCAAT

Product: putative pyruvate decarboxylase protein

Products: NA

Alternate protein names: Indolepyruvate decarboxylase [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MGDTYTVGQYLVDRLRELGLGHLFSVAGDYSIEWVNSYVEKSDIQVIEEVNELNAGYAADGYARLKGIGALCVTYSAGSL
CATNAIAGCYVEKVPVVLINGAPSVQKTLRFEQTGYSSHHFISGRETDRQVFEYITAATVRIDSPHLAPMLIDYALTQCI
TERRPVYVELLEDMVDLECTRPSNALKAAPDISDEDSLNQSIAQISERLQNATKPLIWIGVEIDRFGLHDQAERLIQDLK
IPYVTELLSKAILSEDDVQFAGVFDGQSSSSYVQSLVKDSDFVLALGVWLTDINDLGWPIDLDKTAFASWDTVKYGTIFN
AQVSLADLVNGLIDKRLTCKPQSLPAKTARQAPIVNPTGEITYQGFYDFIQQQIDGNTIVGADASLNYFGSLLLEVGARR
GFIVQSSYSAIGYIGPAATGVSLAKQANQRLLVFAGDGGFQMTAQCLSTQTRFNLNPIIFVMDNGIYGVEQWLADASVFH
GNRPFYTSCILHRWNYSKLAEVFGCRGWKVDTYGELEDAISGAKENLNSPSIIQVVVPQRSIPNNANWKAS

Sequences:

>Translated_551_residues
MGDTYTVGQYLVDRLRELGLGHLFSVAGDYSIEWVNSYVEKSDIQVIEEVNELNAGYAADGYARLKGIGALCVTYSAGSL
CATNAIAGCYVEKVPVVLINGAPSVQKTLRFEQTGYSSHHFISGRETDRQVFEYITAATVRIDSPHLAPMLIDYALTQCI
TERRPVYVELLEDMVDLECTRPSNALKAAPDISDEDSLNQSIAQISERLQNATKPLIWIGVEIDRFGLHDQAERLIQDLK
IPYVTELLSKAILSEDDVQFAGVFDGQSSSSYVQSLVKDSDFVLALGVWLTDINDLGWPIDLDKTAFASWDTVKYGTIFN
AQVSLADLVNGLIDKRLTCKPQSLPAKTARQAPIVNPTGEITYQGFYDFIQQQIDGNTIVGADASLNYFGSLLLEVGARR
GFIVQSSYSAIGYIGPAATGVSLAKQANQRLLVFAGDGGFQMTAQCLSTQTRFNLNPIIFVMDNGIYGVEQWLADASVFH
GNRPFYTSCILHRWNYSKLAEVFGCRGWKVDTYGELEDAISGAKENLNSPSIIQVVVPQRSIPNNANWKAS
>Mature_550_residues
GDTYTVGQYLVDRLRELGLGHLFSVAGDYSIEWVNSYVEKSDIQVIEEVNELNAGYAADGYARLKGIGALCVTYSAGSLC
ATNAIAGCYVEKVPVVLINGAPSVQKTLRFEQTGYSSHHFISGRETDRQVFEYITAATVRIDSPHLAPMLIDYALTQCIT
ERRPVYVELLEDMVDLECTRPSNALKAAPDISDEDSLNQSIAQISERLQNATKPLIWIGVEIDRFGLHDQAERLIQDLKI
PYVTELLSKAILSEDDVQFAGVFDGQSSSSYVQSLVKDSDFVLALGVWLTDINDLGWPIDLDKTAFASWDTVKYGTIFNA
QVSLADLVNGLIDKRLTCKPQSLPAKTARQAPIVNPTGEITYQGFYDFIQQQIDGNTIVGADASLNYFGSLLLEVGARRG
FIVQSSYSAIGYIGPAATGVSLAKQANQRLLVFAGDGGFQMTAQCLSTQTRFNLNPIIFVMDNGIYGVEQWLADASVFHG
NRPFYTSCILHRWNYSKLAEVFGCRGWKVDTYGELEDAISGAKENLNSPSIIQVVVPQRSIPNNANWKAS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG3961

COG function: function code GHR; Pyruvate decarboxylase and related thiamine pyrophosphate-requiring enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=558, Percent_Identity=23.1182795698925, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320123, Length=568, Percent_Identity=27.6408450704225, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323073, Length=564, Percent_Identity=26.7730496453901, Blast_Score=186, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6323163, Length=564, Percent_Identity=26.7730496453901, Blast_Score=183, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6321524, Length=555, Percent_Identity=27.3873873873874, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6320588, Length=561, Percent_Identity=23.5294117647059, Blast_Score=123, Evalue=6e-29,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017764
- InterPro:   IPR012110
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.74 [H]

Molecular weight: Translated: 60612; Mature: 60481

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDTYTVGQYLVDRLRELGLGHLFSVAGDYSIEWVNSYVEKSDIQVIEEVNELNAGYAAD
CCCCEEHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GYARLKGIGALCVTYSAGSLCATNAIAGCYVEKVPVVLINGAPSVQKTLRFEQTGYSSHH
CHHHHHCCCEEEEEECCCCHHHHHHHHHHEEECCCEEEECCCCHHHHHHHHHHCCCCCCC
FISGRETDRQVFEYITAATVRIDSPHLAPMLIDYALTQCITERRPVYVELLEDMVDLECT
EECCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
RPSNALKAAPDISDEDSLNQSIAQISERLQNATKPLIWIGVEIDRFGLHDQAERLIQDLK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCC
IPYVTELLSKAILSEDDVQFAGVFDGQSSSSYVQSLVKDSDFVLALGVWLTDINDLGWPI
CHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCC
DLDKTAFASWDTVKYGTIFNAQVSLADLVNGLIDKRLTCKPQSLPAKTARQAPIVNPTGE
CCCCHHCCCCCCEEECEEEECEEEHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCC
ITYQGFYDFIQQQIDGNTIVGADASLNYFGSLLLEVGARRGFIVQSSYSAIGYIGPAATG
EEEHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCEEECCCHHHH
VSLAKQANQRLLVFAGDGGFQMTAQCLSTQTRFNLNPIIFVMDNGIYGVEQWLADASVFH
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHC
GNRPFYTSCILHRWNYSKLAEVFGCRGWKVDTYGELEDAISGAKENLNSPSIIQVVVPQR
CCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCC
SIPNNANWKAS
CCCCCCCCCCC
>Mature Secondary Structure 
GDTYTVGQYLVDRLRELGLGHLFSVAGDYSIEWVNSYVEKSDIQVIEEVNELNAGYAAD
CCCEEHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GYARLKGIGALCVTYSAGSLCATNAIAGCYVEKVPVVLINGAPSVQKTLRFEQTGYSSHH
CHHHHHCCCEEEEEECCCCHHHHHHHHHHEEECCCEEEECCCCHHHHHHHHHHCCCCCCC
FISGRETDRQVFEYITAATVRIDSPHLAPMLIDYALTQCITERRPVYVELLEDMVDLECT
EECCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
RPSNALKAAPDISDEDSLNQSIAQISERLQNATKPLIWIGVEIDRFGLHDQAERLIQDLK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHCC
IPYVTELLSKAILSEDDVQFAGVFDGQSSSSYVQSLVKDSDFVLALGVWLTDINDLGWPI
CHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCC
DLDKTAFASWDTVKYGTIFNAQVSLADLVNGLIDKRLTCKPQSLPAKTARQAPIVNPTGE
CCCCHHCCCCCCEEECEEEECEEEHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCC
ITYQGFYDFIQQQIDGNTIVGADASLNYFGSLLLEVGARRGFIVQSSYSAIGYIGPAATG
EEEHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCEEECCCHHHH
VSLAKQANQRLLVFAGDGGFQMTAQCLSTQTRFNLNPIIFVMDNGIYGVEQWLADASVFH
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHC
GNRPFYTSCILHRWNYSKLAEVFGCRGWKVDTYGELEDAISGAKENLNSPSIIQVVVPQR
CCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCC
SIPNNANWKAS
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034209 [H]