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The map label for this gene is 86361172

Identifier: 86361172

GI number: 86361172

Start: 494177

End: 495604

Strand: Direct

Name: 86361172

Synonym: RHE_PF00442

Alternate gene names: NA

Gene position: 494177-495604 (Clockwise)

Preceding gene: 86361171

Following gene: 86361173

Centisome position: 76.91

GC content: 60.29

Gene sequence:

>1428_bases
ATGACAGTTGCGCTTTGGAGTCAGTCAATGACGGGTGCGAACCCGGCCCATATCGGCGCCAATGCGCCTACAGTCAAACC
GAGGCCTGCCTTCGCGCAGACGGATCTGGTCGCATTGTCGCGCTATTTTTCTCTTCTGATGATGCGCAACATCACCAGCG
ATGGCTATGTCATCGAGGATCCCACATCGCCCGGCGTCTTTTCGGCCCCGGGCTGCGTCATCGCCGCACCCTCCTATCCA
GCGAACACGCCGGGTGTCGACCAGGACTATGTTTTCAACTGGGTCCGCGACGGGGCCATTACGGCCATCGAGATCGCGCT
TGCCGGCTTGTTACCCATTCCGGGCGGAGTTTTGCCGAGCCTGGTCGACTACGTGAACTTCGCCGCGCTCTGCCAGGCGA
ATGCGAAGAATTCCGACACCGTCACACTTGGCCACGCCTGCTTCACCATCACCGGCGAGGTTCGTCCGTGGTCGGAGCAG
AATGACGGGCCGGCCATTCAGTCGATTGCGATCCTCACCCTGTTCGATCAGCTGGATGGCGCCACGCAGACGATCGCCAA
ACAACTGGTCCAGGCCAACCTCGCTTATCTTCTCGAAGTTTACCAGAACAAGACCACAAATCTCTGGGAGGAATATGAGG
GCTATTCGTTTTTCGCAAGAGCCGTACAGCTCCGCTTTTTCCGGGAAATTTCCACAAACACGATCGGCATTGCGGTGCCT
GCCGGGGTGGCCGGCGCCATCTCCTGGCTGGAAAGCCAGCTGGCAACCCACTGGAACGGGCAGCTCTATGTGAGCATTCT
GGATGCCCTGGAGCAAGCCGGTTACGACGCGAACATCGATATCGTCTCTTCGGTCTGCTATGGCGGGATCGAGCCAACCG
ATACCAAGCTTCTGGCAACGGCGGCCATCTTGCGGCGCCAGTGGGCCGATCCTTCGTCTTCGAACTATTACCCGGTCAAT
GGCGCCGACGCGGCCAAAGGGCTCGGACCACTCTTCGGGCGCTATCCAGGCGACCATTACGATGGCGATGTGGCGGCTCC
GGTTGCCGGCGGGCATCCCTGGGCTCTGTGCACCGCCAATTTCGCCGAGTTTCAATATCGGCTTGCCAATGCCATCGATG
CCAGCGGCGCCATCCCCCTCGATCAGTTCTCCGAACCCTTCTTCGCGGAAATGGGACTTGGCGCATCGAGCAGCGCCGCC
GACGCGTCGGCTGCTTTGCGGGCTTCGTCTGACGCCATGCTGCGCGCCATCGTCTACCACAGCGATTACTATGAACTGAG
CGAACAGTTCGATGGAACCGTTGGCTACGAGAAAAGCGTCCGGAATCTGACCTGGAGCTATGCCTCCTTCCTCTCGGCAG
TCAGAGCCCGCGCCGCCGCCCCGGCCACCAAGTCCAAGCCCCGAAACTCTCGCGGCCCGAAATCATGA

Upstream 100 bases:

>100_bases
CACTCTCGACACCCATTCGGCCGCAATATTTCGCTCATCCGGTCCGGAATATTTTGTCGATCTGGTCCCGGCAAGTCTCA
ACCGATCTTGGAGTCTGTCA

Downstream 100 bases:

>100_bases
CGATTGAAGGCCGTAGGGCCTGATCTCGATTGAGTCCAGAACGCATGATCCGCAAAGCCCTGATCGGCTTTCCGGGATTA
ATGGAGGACCAAAGTGCTTT

Product: putative glucoamylase protein

Products: D-glucuronate; alcohol

Alternate protein names: Glucoamylase Protein; Glucan 1 4-Alpha-Glucosidase

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTVALWSQSMTGANPAHIGANAPTVKPRPAFAQTDLVALSRYFSLLMMRNITSDGYVIEDPTSPGVFSAPGCVIAAPSYP
ANTPGVDQDYVFNWVRDGAITAIEIALAGLLPIPGGVLPSLVDYVNFAALCQANAKNSDTVTLGHACFTITGEVRPWSEQ
NDGPAIQSIAILTLFDQLDGATQTIAKQLVQANLAYLLEVYQNKTTNLWEEYEGYSFFARAVQLRFFREISTNTIGIAVP
AGVAGAISWLESQLATHWNGQLYVSILDALEQAGYDANIDIVSSVCYGGIEPTDTKLLATAAILRRQWADPSSSNYYPVN
GADAAKGLGPLFGRYPGDHYDGDVAAPVAGGHPWALCTANFAEFQYRLANAIDASGAIPLDQFSEPFFAEMGLGASSSAA
DASAALRASSDAMLRAIVYHSDYYELSEQFDGTVGYEKSVRNLTWSYASFLSAVRARAAAPATKSKPRNSRGPKS

Sequences:

>Translated_475_residues
MTVALWSQSMTGANPAHIGANAPTVKPRPAFAQTDLVALSRYFSLLMMRNITSDGYVIEDPTSPGVFSAPGCVIAAPSYP
ANTPGVDQDYVFNWVRDGAITAIEIALAGLLPIPGGVLPSLVDYVNFAALCQANAKNSDTVTLGHACFTITGEVRPWSEQ
NDGPAIQSIAILTLFDQLDGATQTIAKQLVQANLAYLLEVYQNKTTNLWEEYEGYSFFARAVQLRFFREISTNTIGIAVP
AGVAGAISWLESQLATHWNGQLYVSILDALEQAGYDANIDIVSSVCYGGIEPTDTKLLATAAILRRQWADPSSSNYYPVN
GADAAKGLGPLFGRYPGDHYDGDVAAPVAGGHPWALCTANFAEFQYRLANAIDASGAIPLDQFSEPFFAEMGLGASSSAA
DASAALRASSDAMLRAIVYHSDYYELSEQFDGTVGYEKSVRNLTWSYASFLSAVRARAAAPATKSKPRNSRGPKS
>Mature_474_residues
TVALWSQSMTGANPAHIGANAPTVKPRPAFAQTDLVALSRYFSLLMMRNITSDGYVIEDPTSPGVFSAPGCVIAAPSYPA
NTPGVDQDYVFNWVRDGAITAIEIALAGLLPIPGGVLPSLVDYVNFAALCQANAKNSDTVTLGHACFTITGEVRPWSEQN
DGPAIQSIAILTLFDQLDGATQTIAKQLVQANLAYLLEVYQNKTTNLWEEYEGYSFFARAVQLRFFREISTNTIGIAVPA
GVAGAISWLESQLATHWNGQLYVSILDALEQAGYDANIDIVSSVCYGGIEPTDTKLLATAAILRRQWADPSSSNYYPVNG
ADAAKGLGPLFGRYPGDHYDGDVAAPVAGGHPWALCTANFAEFQYRLANAIDASGAIPLDQFSEPFFAEMGLGASSSAAD
ASAALRASSDAMLRAIVYHSDYYELSEQFDGTVGYEKSVRNLTWSYASFLSAVRARAAAPATKSKPRNSRGPKS

Specific function: Unknown

COG id: COG3387

COG function: function code G; Glucoamylase and related glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Saccharomyces cerevisiae, GI6322092, Length=179, Percent_Identity=29.608938547486, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.1.3

Molecular weight: Translated: 50778; Mature: 50647

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVALWSQSMTGANPAHIGANAPTVKPRPAFAQTDLVALSRYFSLLMMRNITSDGYVIED
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
PTSPGVFSAPGCVIAAPSYPANTPGVDQDYVFNWVRDGAITAIEIALAGLLPIPGGVLPS
CCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
LVDYVNFAALCQANAKNSDTVTLGHACFTITGEVRPWSEQNDGPAIQSIAILTLFDQLDG
HHHHHHHHHHHHCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCC
ATQTIAKQLVQANLAYLLEVYQNKTTNLWEEYEGYSFFARAVQLRFFREISTNTIGIAVP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEC
AGVAGAISWLESQLATHWNGQLYVSILDALEQAGYDANIDIVSSVCYGGIEPTDTKLLAT
CCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHH
AAILRRQWADPSSSNYYPVNGADAAKGLGPLFGRYPGDHYDGDVAAPVAGGHPWALCTAN
HHHHHHHCCCCCCCCEECCCCCHHHHCCCHHHHCCCCCCCCCCEECCCCCCCCEEEEECH
FAEFQYRLANAIDASGAIPLDQFSEPFFAEMGLGASSSAADASAALRASSDAMLRAIVYH
HHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHH
SDYYELSEQFDGTVGYEKSVRNLTWSYASFLSAVRARAAAPATKSKPRNSRGPKS
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVALWSQSMTGANPAHIGANAPTVKPRPAFAQTDLVALSRYFSLLMMRNITSDGYVIED
EEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
PTSPGVFSAPGCVIAAPSYPANTPGVDQDYVFNWVRDGAITAIEIALAGLLPIPGGVLPS
CCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
LVDYVNFAALCQANAKNSDTVTLGHACFTITGEVRPWSEQNDGPAIQSIAILTLFDQLDG
HHHHHHHHHHHHCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCC
ATQTIAKQLVQANLAYLLEVYQNKTTNLWEEYEGYSFFARAVQLRFFREISTNTIGIAVP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEC
AGVAGAISWLESQLATHWNGQLYVSILDALEQAGYDANIDIVSSVCYGGIEPTDTKLLAT
CCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHH
AAILRRQWADPSSSNYYPVNGADAAKGLGPLFGRYPGDHYDGDVAAPVAGGHPWALCTAN
HHHHHHHCCCCCCCCEECCCCCHHHHCCCHHHHCCCCCCCCCCEECCCCCCCCEEEEECH
FAEFQYRLANAIDASGAIPLDQFSEPFFAEMGLGASSSAADASAALRASSDAMLRAIVYH
HHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHH
SDYYELSEQFDGTVGYEKSVRNLTWSYASFLSAVRARAAAPATKSKPRNSRGPKS
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; beta-D-glucuronoside

Specific reaction: H2O + a beta-D-glucuronoside = D-glucuronate + an alcohol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA