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The map label for this gene is thcR [H]

Identifier: 86361144

GI number: 86361144

Start: 466997

End: 467995

Strand: Direct

Name: thcR [H]

Synonym: RHE_PF00414

Alternate gene names: 86361144

Gene position: 466997-467995 (Clockwise)

Preceding gene: 86361142

Following gene: 86361148

Centisome position: 72.68

GC content: 61.46

Gene sequence:

>999_bases
ATGACGGAAAACAGCGCCCAGCCATTCTCCTTCCGATTCCACGGTTCGTCTTTCGACAATATGGTGGAAACCCTCGGCGG
CGCTTTCGGCGCGTTTGATGCCGAGCCTGTCGGCCGCGCCAAGGACTTCCACTGGGGATTGGATTTTTCGGTGGCCGAGA
CCGCCGTTCTGCTGACGGGCTACCACGAGGCGGAGTTCCAATTCAACGTCGAGCCGACCGTCAACACGGCAGAGTATCTG
TCGCTCGTGGTGCCGCGCCGCGGCGGCATGGGAGTTACGTATGGGGCGCGCAAGGCCGAGGCCGGGCAAGGCAAACTGCT
TCTCTACAATAATTTCGAACCCGACAGCGTACTCATGCACGGGCAAGGCAATGTCATCGACGAGTTGCTGATCAACTGGC
CCGTCATCCTCCAGACGATCGGCCAGACGTTCGAAATGCCGTTCAGCGGCTCGCTCGACCTTCTGCCCGAACTGGATCTG
TCGACGCCGGCCGGCCGGACGATCGGCAACCTCACCGAAACGATCATATCAGGGATGCGCGACAACGGCCCGCTGCTGCA
GTCGCCGATCGCCATGGCGCACATGACGCAGGCGCTCGCCGATCTCGTCGTGCGCATGGTGCCGCACCGGCTGTCGCATT
TCCTCGAAAAGGGAACCGCTCTGATTGCTCCGAAGCATGTGCGCAGGGCGATCGAGTTCATGCAGGCCAATATCGATCAG
CCGATCACCATGCCGAAGGTGGCGGAAGCGGCCGGCGTCTCCAGCAGAGCGCTCGAAACCGGTTTCCGCGCATTCAAGGG
AACGTCCCCCGCCGCTTACCTGCTGACGCTTCGTCTGCGCGCAGCGCGCCGGGATCTGCTCGATCCCGAGTGCAGGGAGA
CGATGAAGGCCATCTGTCTCAAATGGGGATTCTTTCATTTCGGCCGCTTTTCCGCGGTCTACAAGGCGACATATGGCGAA
TACCCCTCCGATACGAGAAAGCGCATGGGCCGCCGGTAA

Upstream 100 bases:

>100_bases
AAAGGCGCGAGGAGAAAGATGAGCTCGGCGACGCCCACGCCGACGATGCGGCCGAGGCATCCCGCCGTGTCCGGCGGCAG
ATGAAATAAAGGGGGAAAGT

Downstream 100 bases:

>100_bases
TTTGCATCTGAAGCGCACCGCGATCTTTCAGCTTCGCTCTTTGCGCCCTCTGTCTTTTAAAGCACCGCCAACCTGTAGTG
CACTCGCATCGTCCTCGCAT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTENSAQPFSFRFHGSSFDNMVETLGGAFGAFDAEPVGRAKDFHWGLDFSVAETAVLLTGYHEAEFQFNVEPTVNTAEYL
SLVVPRRGGMGVTYGARKAEAGQGKLLLYNNFEPDSVLMHGQGNVIDELLINWPVILQTIGQTFEMPFSGSLDLLPELDL
STPAGRTIGNLTETIISGMRDNGPLLQSPIAMAHMTQALADLVVRMVPHRLSHFLEKGTALIAPKHVRRAIEFMQANIDQ
PITMPKVAEAAGVSSRALETGFRAFKGTSPAAYLLTLRLRAARRDLLDPECRETMKAICLKWGFFHFGRFSAVYKATYGE
YPSDTRKRMGRR

Sequences:

>Translated_332_residues
MTENSAQPFSFRFHGSSFDNMVETLGGAFGAFDAEPVGRAKDFHWGLDFSVAETAVLLTGYHEAEFQFNVEPTVNTAEYL
SLVVPRRGGMGVTYGARKAEAGQGKLLLYNNFEPDSVLMHGQGNVIDELLINWPVILQTIGQTFEMPFSGSLDLLPELDL
STPAGRTIGNLTETIISGMRDNGPLLQSPIAMAHMTQALADLVVRMVPHRLSHFLEKGTALIAPKHVRRAIEFMQANIDQ
PITMPKVAEAAGVSSRALETGFRAFKGTSPAAYLLTLRLRAARRDLLDPECRETMKAICLKWGFFHFGRFSAVYKATYGE
YPSDTRKRMGRR
>Mature_331_residues
TENSAQPFSFRFHGSSFDNMVETLGGAFGAFDAEPVGRAKDFHWGLDFSVAETAVLLTGYHEAEFQFNVEPTVNTAEYLS
LVVPRRGGMGVTYGARKAEAGQGKLLLYNNFEPDSVLMHGQGNVIDELLINWPVILQTIGQTFEMPFSGSLDLLPELDLS
TPAGRTIGNLTETIISGMRDNGPLLQSPIAMAHMTQALADLVVRMVPHRLSHFLEKGTALIAPKHVRRAIEFMQANIDQP
ITMPKVAEAAGVSSRALETGFRAFKGTSPAAYLLTLRLRAARRDLLDPECRETMKAICLKWGFFHFGRFSAVYKATYGEY
PSDTRKRMGRR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=103, Percent_Identity=36.8932038834951, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 36723; Mature: 36591

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENSAQPFSFRFHGSSFDNMVETLGGAFGAFDAEPVGRAKDFHWGLDFSVAETAVLLTG
CCCCCCCCEEEEECCCCHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEC
YHEAEFQFNVEPTVNTAEYLSLVVPRRGGMGVTYGARKAEAGQGKLLLYNNFEPDSVLMH
CCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEC
GQGNVIDELLINWPVILQTIGQTFEMPFSGSLDLLPELDLSTPAGRTIGNLTETIISGMR
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
DNGPLLQSPIAMAHMTQALADLVVRMVPHRLSHFLEKGTALIAPKHVRRAIEFMQANIDQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHCCCC
PITMPKVAEAAGVSSRALETGFRAFKGTSPAAYLLTLRLRAARRDLLDPECRETMKAICL
CCCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KWGFFHFGRFSAVYKATYGEYPSDTRKRMGRR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TENSAQPFSFRFHGSSFDNMVETLGGAFGAFDAEPVGRAKDFHWGLDFSVAETAVLLTG
CCCCCCCEEEEECCCCHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCHHHHHEEEEEC
YHEAEFQFNVEPTVNTAEYLSLVVPRRGGMGVTYGARKAEAGQGKLLLYNNFEPDSVLMH
CCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEC
GQGNVIDELLINWPVILQTIGQTFEMPFSGSLDLLPELDLSTPAGRTIGNLTETIISGMR
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
DNGPLLQSPIAMAHMTQALADLVVRMVPHRLSHFLEKGTALIAPKHVRRAIEFMQANIDQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHCCCC
PITMPKVAEAAGVSSRALETGFRAFKGTSPAAYLLTLRLRAARRDLLDPECRETMKAICL
CCCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KWGFFHFGRFSAVYKATYGEYPSDTRKRMGRR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]