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The map label for this gene is opuAC [H]

Identifier: 86361125

GI number: 86361125

Start: 447496

End: 448347

Strand: Reverse

Name: opuAC [H]

Synonym: RHE_PF00395

Alternate gene names: 86361125

Gene position: 448347-447496 (Counterclockwise)

Preceding gene: 86361126

Following gene: 86361124

Centisome position: 69.78

GC content: 58.33

Gene sequence:

>852_bases
ATGAAGACTTTGTGGAAGGCTCTCTGCGCCGCTGCGGTGATCGGGATGAGCATCCTGCCTGCCCGTGCGGAGGAAAAGAC
GATCACTCTCGGCACGATGTCGTGGGAAGACCTGACGCCGATCACCGGCATCACCAAGAAGGTTCTGGAAGACGCCGGCT
ATACCGTGAAGGTCACCGAATTTTCCGAATGGGGCATTGCCTATGCCGCTCTCGCGAAGGGCGATATCCAGGCTCTGACC
TCGCAGACCGATTACGTCGCTCAGGACTATTGGGACAAGAACAAGAACCGCCTCGAGAAGATTTCTCCGGTTTCGCATGG
TCTTTATCAGGGCGTGGCCGTTCCGAAATATGTCCCGATCGACTCGCTCGAACAGCTCAATGAAAACGCCGACAAGTTCG
GCGGCAAGATCATCGGCATCGAACCGGGCGCCGGCCTGATGCGCGACACGTCGAATGCAGTCAAGGAATATGGCCTCAAG
CTCCAGCTCGTCGAAGGCAGCACGGCGGCGATGACGGCGGCGCTGAAGTCTGCTTACGACCGCCAGGAATGGATTGCGGT
CACGATCTGGGAGCCGTCGTGGATGGTCCAGAAGTACGAGGTCAAGTATCTCAAGGATCCGAAGGGCGTCTTCCCGCCGC
CGCAGAGCTACTACTGGATCGGCCATAAGGGCTTCTCCGAAGAGTACCCGCATGCGCGCGAAGTCATGGCTAGCGTCTAC
GTGCCGATCGCCGACATCACCACCATCAACGGTGAAGTCAAGGACGGCAAGACGATGGACCAGGCCGTGCAGGACTGGAT
CGGCGCCCATGCCGACCTGATCAAGCGCTGGGAAAACATCAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
GCGTGCGGGCCGCGAGGGATGAACCCTCGGCTCACGCAATAGGAAGACTGCCGCCCGCAGGCTTCAATGAGAATGGGAAC
GAAAACCAGGAGTAACAACA

Downstream 100 bases:

>100_bases
ATGTCGCGTGGCCGTCCGAACTCTCGGGCGGCCACCCTAAAATCTAAGAAAGAAGCCAGCGCCACTGGCTCAAAGGGGAT
CGCTATGAAAACCGCCAATA

Product: glycine betaine/L-proline ABC transporter, substrate-binding protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKTLWKALCAAAVIGMSILPARAEEKTITLGTMSWEDLTPITGITKKVLEDAGYTVKVTEFSEWGIAYAALAKGDIQALT
SQTDYVAQDYWDKNKNRLEKISPVSHGLYQGVAVPKYVPIDSLEQLNENADKFGGKIIGIEPGAGLMRDTSNAVKEYGLK
LQLVEGSTAAMTAALKSAYDRQEWIAVTIWEPSWMVQKYEVKYLKDPKGVFPPPQSYYWIGHKGFSEEYPHAREVMASVY
VPIADITTINGEVKDGKTMDQAVQDWIGAHADLIKRWENIKKK

Sequences:

>Translated_283_residues
MKTLWKALCAAAVIGMSILPARAEEKTITLGTMSWEDLTPITGITKKVLEDAGYTVKVTEFSEWGIAYAALAKGDIQALT
SQTDYVAQDYWDKNKNRLEKISPVSHGLYQGVAVPKYVPIDSLEQLNENADKFGGKIIGIEPGAGLMRDTSNAVKEYGLK
LQLVEGSTAAMTAALKSAYDRQEWIAVTIWEPSWMVQKYEVKYLKDPKGVFPPPQSYYWIGHKGFSEEYPHAREVMASVY
VPIADITTINGEVKDGKTMDQAVQDWIGAHADLIKRWENIKKK
>Mature_283_residues
MKTLWKALCAAAVIGMSILPARAEEKTITLGTMSWEDLTPITGITKKVLEDAGYTVKVTEFSEWGIAYAALAKGDIQALT
SQTDYVAQDYWDKNKNRLEKISPVSHGLYQGVAVPKYVPIDSLEQLNENADKFGGKIIGIEPGAGLMRDTSNAVKEYGLK
LQLVEGSTAAMTAALKSAYDRQEWIAVTIWEPSWMVQKYEVKYLKDPKGVFPPPQSYYWIGHKGFSEEYPHAREVMASVY
VPIADITTINGEVKDGKTMDQAVQDWIGAHADLIKRWENIKKK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine [H]

COG id: COG2113

COG function: function code E; ABC-type proline/glycine betaine transport systems, periplasmic components

Gene ontology:

Cell location: Cell membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007210 [H]

Pfam domain/function: PF04069 OpuAC [H]

EC number: NA

Molecular weight: Translated: 31554; Mature: 31554

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLWKALCAAAVIGMSILPARAEEKTITLGTMSWEDLTPITGITKKVLEDAGYTVKVTE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEE
FSEWGIAYAALAKGDIQALTSQTDYVAQDYWDKNKNRLEKISPVSHGLYQGVAVPKYVPI
CCCCCHHHHHHHCCHHHHHHCCHHHHHHHHHCCCHHHHHHHCHHHHHHHHCCCCCCCCCH
DSLEQLNENADKFGGKIIGIEPGAGLMRDTSNAVKEYGLKLQLVEGSTAAMTAALKSAYD
HHHHHHHCCHHHHCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCC
RQEWIAVTIWEPSWMVQKYEVKYLKDPKGVFPPPQSYYWIGHKGFSEEYPHAREVMASVY
CCCEEEEEEECCCHHHHHEEHEEECCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHH
VPIADITTINGEVKDGKTMDQAVQDWIGAHADLIKRWENIKKK
CCHHHEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTLWKALCAAAVIGMSILPARAEEKTITLGTMSWEDLTPITGITKKVLEDAGYTVKVTE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEE
FSEWGIAYAALAKGDIQALTSQTDYVAQDYWDKNKNRLEKISPVSHGLYQGVAVPKYVPI
CCCCCHHHHHHHCCHHHHHHCCHHHHHHHHHCCCHHHHHHHCHHHHHHHHCCCCCCCCCH
DSLEQLNENADKFGGKIIGIEPGAGLMRDTSNAVKEYGLKLQLVEGSTAAMTAALKSAYD
HHHHHHHCCHHHHCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCC
RQEWIAVTIWEPSWMVQKYEVKYLKDPKGVFPPPQSYYWIGHKGFSEEYPHAREVMASVY
CCCEEEEEEECCCHHHHHEEHEEECCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHH
VPIADITTINGEVKDGKTMDQAVQDWIGAHADLIKRWENIKKK
CCHHHEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]