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The map label for this gene is ycsA [H]

Identifier: 86361069

GI number: 86361069

Start: 380126

End: 381169

Strand: Direct

Name: ycsA [H]

Synonym: RHE_PF00339

Alternate gene names: 86361069

Gene position: 380126-381169 (Clockwise)

Preceding gene: 86361068

Following gene: 86361070

Centisome position: 59.16

GC content: 62.36

Gene sequence:

>1044_bases
ATGAAGACCTACAGGATTGCCCTTCTGCCAGGAGACGGCATCGGCCGCGACGTGACCGACGCTGCCTGGGCCGTGCTCGA
AAAAACAGCTGGATCGGGCGGATTTTCCCTTGAGGCAACCCGCTACCCCTGGTCCTGCGACTACTATCTCGAAAACGGCA
GCATGATGCCCGCCGATGGGATCGAGACGCTGAAGTCTTTCGACGCCATCCTGCTCGGCGCCGTCGGATGGCCCCGAAAA
GTGCCGGATTCCGTATCGCTGCACGGCCTCCTGCTGCCGATCCGCAAGGCCTTCGTGCAATATGCCAATATCCGCCCGCA
CCGTTTGCTCCCAGGCGTGCAGGGACCGCTGCGGTCTGACGGTTTCGACATTCTCTGCATTCGTGAAAACACCGAGGGCG
AATATTCCGGCGCCGGCGGCCGTATCCATCAGGGCGCGGACAACGAAGTGGCGATCGAAACCGCGATCTTCACCCGCAAG
GGGGTCGAACGCATCCTGCGTTTCGGCTTCGAGCAGGCACGTGCGCGACGCGGCAAGCTTGCCTCGGTGACGAAGTCGAA
CGCGCAGAAATATTCGATGGTCTTCTGGGACGAGATCACCCACGGGCTCTCTGCGGAATATCCCGATGTCGAAGTGACCA
GCTATCATATAGACGCCATGGCCGCCCGCATGGTGATGGCGCCCGAAAGCCTCGATGTCGTGGCCGCCTCCAACCTGTTC
GGCGACATCCTGACCGACCTTGGCGCCGCGATCCAGGGCGGGCTCGGCTTTGCCGCATCCGCCAACATCAATCCCGATCG
ATCGGCGCCCTCCATGTTCGAACCAGTCCACGGGTCAGCGCCCGATATCGCTCATCTCGGGATCGCCAATCCGATCGCCG
CCATCTGGTCGGGTGCAATGATGCTGGAACATCTCGGAGAAACGGCCGCCGCCGAAAAGGTGATGGACGCAATCGAAACG
ACCACTGCGCGCGGCGTCGGCGCAATTCCCGGCAAGGACAGGACCGACGCGATCACGGCATCGGTGCTTTCGGCGCTCGG
CTAG

Upstream 100 bases:

>100_bases
CGGCTACAAGCAATCCGGAATCGGCAAGGATCTCGGCCGCGAAGCCTATCATGCCAACCGCAAGAGCAAGAGCGTGCTCA
TCAGCCTTTAAGGAGCTCAG

Downstream 100 bases:

>100_bases
CGCGTCGCGCAAACTGGAAGTGATTTGGAAAAGTTTTGACGAAAGGGGAGAAAATGAACGGATTGCGAGACAGTAGCCTG
CTTCGCCAGCAAGGTCTCAT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: TDH; D-malate dehydrogenase [decarboxylating] [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKTYRIALLPGDGIGRDVTDAAWAVLEKTAGSGGFSLEATRYPWSCDYYLENGSMMPADGIETLKSFDAILLGAVGWPRK
VPDSVSLHGLLLPIRKAFVQYANIRPHRLLPGVQGPLRSDGFDILCIRENTEGEYSGAGGRIHQGADNEVAIETAIFTRK
GVERILRFGFEQARARRGKLASVTKSNAQKYSMVFWDEITHGLSAEYPDVEVTSYHIDAMAARMVMAPESLDVVAASNLF
GDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIAHLGIANPIAAIWSGAMMLEHLGETAAAEKVMDAIET
TTARGVGAIPGKDRTDAITASVLSALG

Sequences:

>Translated_347_residues
MKTYRIALLPGDGIGRDVTDAAWAVLEKTAGSGGFSLEATRYPWSCDYYLENGSMMPADGIETLKSFDAILLGAVGWPRK
VPDSVSLHGLLLPIRKAFVQYANIRPHRLLPGVQGPLRSDGFDILCIRENTEGEYSGAGGRIHQGADNEVAIETAIFTRK
GVERILRFGFEQARARRGKLASVTKSNAQKYSMVFWDEITHGLSAEYPDVEVTSYHIDAMAARMVMAPESLDVVAASNLF
GDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIAHLGIANPIAAIWSGAMMLEHLGETAAAEKVMDAIET
TTARGVGAIPGKDRTDAITASVLSALG
>Mature_347_residues
MKTYRIALLPGDGIGRDVTDAAWAVLEKTAGSGGFSLEATRYPWSCDYYLENGSMMPADGIETLKSFDAILLGAVGWPRK
VPDSVSLHGLLLPIRKAFVQYANIRPHRLLPGVQGPLRSDGFDILCIRENTEGEYSGAGGRIHQGADNEVAIETAIFTRK
GVERILRFGFEQARARRGKLASVTKSNAQKYSMVFWDEITHGLSAEYPDVEVTSYHIDAMAARMVMAPESLDVVAASNLF
GDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIAHLGIANPIAAIWSGAMMLEHLGETAAAEKVMDAIET
TTARGVGAIPGKDRTDAITASVLSALG

Specific function: Unknown

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=327, Percent_Identity=33.0275229357798, Blast_Score=147, Evalue=9e-36,
Organism=Homo sapiens, GI4758582, Length=317, Percent_Identity=27.1293375394322, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI28178816, Length=323, Percent_Identity=26.3157894736842, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI28178838, Length=317, Percent_Identity=27.1293375394322, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI28178821, Length=322, Percent_Identity=26.0869565217391, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI28178819, Length=165, Percent_Identity=29.0909090909091, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788101, Length=335, Percent_Identity=52.2388059701493, Blast_Score=340, Evalue=5e-95,
Organism=Escherichia coli, GI87081683, Length=335, Percent_Identity=38.8059701492537, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1787381, Length=366, Percent_Identity=27.3224043715847, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=345, Percent_Identity=32.1739130434783, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25144293, Length=346, Percent_Identity=28.9017341040462, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=308, Percent_Identity=27.9220779220779, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17505779, Length=317, Percent_Identity=28.391167192429, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324709, Length=355, Percent_Identity=32.6760563380282, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322097, Length=362, Percent_Identity=33.4254143646409, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=31.728045325779, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319830, Length=347, Percent_Identity=32.5648414985591, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24643268, Length=354, Percent_Identity=31.0734463276836, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24643270, Length=354, Percent_Identity=31.0734463276836, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24661184, Length=325, Percent_Identity=32.6153846153846, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI161078633, Length=258, Percent_Identity=29.0697674418605, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24650122, Length=258, Percent_Identity=29.0697674418605, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI161078639, Length=258, Percent_Identity=28.2945736434109, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078637, Length=258, Percent_Identity=29.0697674418605, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078635, Length=258, Percent_Identity=29.0697674418605, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281362242, Length=319, Percent_Identity=26.9592476489028, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648872, Length=319, Percent_Identity=26.9592476489028, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI20130355, Length=326, Percent_Identity=24.5398773006135, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.93; =4.1.1.73; =1.1.1.83 [H]

Molecular weight: Translated: 36960; Mature: 36960

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYRIALLPGDGIGRDVTDAAWAVLEKTAGSGGFSLEATRYPWSCDYYLENGSMMPADG
CCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCHH
IETLKSFDAILLGAVGWPRKVPDSVSLHGLLLPIRKAFVQYANIRPHRLLPGVQGPLRSD
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC
GFDILCIRENTEGEYSGAGGRIHQGADNEVAIETAIFTRKGVERILRFGFEQARARRGKL
CEEEEEEEECCCCCCCCCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHCCCH
ASVTKSNAQKYSMVFWDEITHGLSAEYPDVEVTSYHIDAMAARMVMAPESLDVVAASNLF
HHHHHCCCHHEEEEEHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHH
GDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIAHLGIANPIAAIWSGAM
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHCCCCCCCCCHHHCCCCHHHHHHHHHHH
MLEHLGETAAAEKVMDAIETTTARGVGAIPGKDRTDAITASVLSALG
HHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTYRIALLPGDGIGRDVTDAAWAVLEKTAGSGGFSLEATRYPWSCDYYLENGSMMPADG
CCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCHH
IETLKSFDAILLGAVGWPRKVPDSVSLHGLLLPIRKAFVQYANIRPHRLLPGVQGPLRSD
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC
GFDILCIRENTEGEYSGAGGRIHQGADNEVAIETAIFTRKGVERILRFGFEQARARRGKL
CEEEEEEEECCCCCCCCCCCCEECCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHCCCH
ASVTKSNAQKYSMVFWDEITHGLSAEYPDVEVTSYHIDAMAARMVMAPESLDVVAASNLF
HHHHHCCCHHEEEEEHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHH
GDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIAHLGIANPIAAIWSGAM
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHCCCCCCCCCHHHCCCCHHHHHHHHHHH
MLEHLGETAAAEKVMDAIETTTARGVGAIPGKDRTDAITASVLSALG
HHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]