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The map label for this gene is ghrA [H]

Identifier: 86361066

GI number: 86361066

Start: 377157

End: 378080

Strand: Direct

Name: ghrA [H]

Synonym: RHE_PF00336

Alternate gene names: 86361066

Gene position: 377157-378080 (Clockwise)

Preceding gene: 86361065

Following gene: 86361068

Centisome position: 58.7

GC content: 64.61

Gene sequence:

>924_bases
ATGACCTTTCTCTTCAATTCCGATGCGAAGCGCGGCGCTATATTCGCCGAGGCCTTCGCGCGCGAACTGCCCGACATTGC
CTTCGCCATGGATCCGGAATCGGTCGAACCGGAGGCGGTGCGCTACCTAATCACCTGGACTGTGCCGGATGATCTCGCCC
GCTACCGCAATCTCGAAATCTTGTTTTCGATCGGCGCCGGCATCGATCAGTTTCGCATCGACGCCGTGCCGGCTCCGGTC
AAGATAGTGCGCATGGTCGAGGATAGCATCGTCAAAATGATGCAGGAATATGCGACGCTGGCGGTCCTCGCTCTCCATCG
CGATCTGCCGGCCTATCTCGACCAGCAGCGGCGCGGGATCTGGCAACCGATCGCACCCGTTCAGGCCGCAGAACGCCGCG
TCGGCGTGCTCGGGCTCGGAATGCTGGGAAGGGCGGTGCTTGAGAGTTTGCGCCCATTCGGTTTGCCGCTGTCCGGCTGG
AGCCGCGCTCCGCGCGCGATCGACAGCGTTCGATGCCTCAGCGGCGGCGAGGGACTCGAGACCCTGCTTGCCACCACCGA
TATTCTCGTCTGCATGCTGCCGCTGACGGAGGAGACGCGGGGCTTCCTGAATGCCGAGCTCTTCGCAAGGCTTCCCGCTG
GTGCCGCACTCGCTCATGTCGGTCGCGGCGCCCAGCTCGATCATCAGGCGCTCGTCGCTGCGCTTGACGCCGGACATCTC
TCCGGCGCCGTGGTCGATGTTACCGATCCGGAGCCGCTGCCTCCGGGCCATGCCTTCTGGAATCATCCCAAGATTCTGCT
GACGCCGCATATTGCCAGCGTCACTCAAGCCGAGCCCGCGGCGGCGGCGGTGATCGGCAACATCAAACGGCACCGGGCGG
GCCTCGACCCGATCGGGCTGGTCGATCGCGGCCGCGGCTACTGA

Upstream 100 bases:

>100_bases
CGAATTCATCCTCGCGAACGAACTCCTGGCCTGCCAGGCGATGATCGAGGCACTCGCCCGACGTTGCATCGCCTGACCGT
CAGATCAAGGAGCCGATGCA

Downstream 100 bases:

>100_bases
TTTATCTCACTACAAATCTGCACCCTGGAAAGGAAACCCATGTCCCTGCTCAAGACGATCGATACCAACCCGTCCCAAGC
GCCGCGCGAGTCCGGTCCCC

Product: putative D-2-hydroxyacid dehydrogenase protein

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTFLFNSDAKRGAIFAEAFARELPDIAFAMDPESVEPEAVRYLITWTVPDDLARYRNLEILFSIGAGIDQFRIDAVPAPV
KIVRMVEDSIVKMMQEYATLAVLALHRDLPAYLDQQRRGIWQPIAPVQAAERRVGVLGLGMLGRAVLESLRPFGLPLSGW
SRAPRAIDSVRCLSGGEGLETLLATTDILVCMLPLTEETRGFLNAELFARLPAGAALAHVGRGAQLDHQALVAALDAGHL
SGAVVDVTDPEPLPPGHAFWNHPKILLTPHIASVTQAEPAAAAVIGNIKRHRAGLDPIGLVDRGRGY

Sequences:

>Translated_307_residues
MTFLFNSDAKRGAIFAEAFARELPDIAFAMDPESVEPEAVRYLITWTVPDDLARYRNLEILFSIGAGIDQFRIDAVPAPV
KIVRMVEDSIVKMMQEYATLAVLALHRDLPAYLDQQRRGIWQPIAPVQAAERRVGVLGLGMLGRAVLESLRPFGLPLSGW
SRAPRAIDSVRCLSGGEGLETLLATTDILVCMLPLTEETRGFLNAELFARLPAGAALAHVGRGAQLDHQALVAALDAGHL
SGAVVDVTDPEPLPPGHAFWNHPKILLTPHIASVTQAEPAAAAVIGNIKRHRAGLDPIGLVDRGRGY
>Mature_306_residues
TFLFNSDAKRGAIFAEAFARELPDIAFAMDPESVEPEAVRYLITWTVPDDLARYRNLEILFSIGAGIDQFRIDAVPAPVK
IVRMVEDSIVKMMQEYATLAVLALHRDLPAYLDQQRRGIWQPIAPVQAAERRVGVLGLGMLGRAVLESLRPFGLPLSGWS
RAPRAIDSVRCLSGGEGLETLLATTDILVCMLPLTEETRGFLNAELFARLPAGAALAHVGRGAQLDHQALVAALDAGHLS
GAVVDVTDPEPLPPGHAFWNHPKILLTPHIASVTQAEPAAAAVIGNIKRHRAGLDPIGLVDRGRGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=226, Percent_Identity=27.8761061946903, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI23308577, Length=236, Percent_Identity=25.4237288135593, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI61743967, Length=256, Percent_Identity=26.953125, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4557497, Length=256, Percent_Identity=26.953125, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI145580578, Length=256, Percent_Identity=25.390625, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4557499, Length=256, Percent_Identity=25.390625, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=32.9073482428115, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87082289, Length=220, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789279, Length=187, Percent_Identity=26.7379679144385, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=245, Percent_Identity=27.3469387755102, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25147481, Length=278, Percent_Identity=26.2589928057554, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324055, Length=235, Percent_Identity=25.9574468085106, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324964, Length=258, Percent_Identity=26.3565891472868, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320925, Length=283, Percent_Identity=23.321554770318, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322116, Length=198, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28574286, Length=152, Percent_Identity=33.5526315789474, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45552429, Length=165, Percent_Identity=31.5151515151515, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585514, Length=165, Percent_Identity=31.5151515151515, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28574282, Length=165, Percent_Identity=31.5151515151515, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28574284, Length=165, Percent_Identity=31.5151515151515, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45551003, Length=165, Percent_Identity=31.5151515151515, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19921140, Length=229, Percent_Identity=27.0742358078603, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28571528, Length=223, Percent_Identity=26.0089686098655, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24646446, Length=281, Percent_Identity=27.0462633451957, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646448, Length=281, Percent_Identity=27.0462633451957, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646452, Length=281, Percent_Identity=27.0462633451957, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646450, Length=281, Percent_Identity=27.0462633451957, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585516, Length=142, Percent_Identity=29.5774647887324, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI62472511, Length=281, Percent_Identity=27.0462633451957, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33207; Mature: 33076

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLFNSDAKRGAIFAEAFARELPDIAFAMDPESVEPEAVRYLITWTVPDDLARYRNLEI
CEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEECCHHHHHHCCCEE
LFSIGAGIDQFRIDAVPAPVKIVRMVEDSIVKMMQEYATLAVLALHRDLPAYLDQQRRGI
EEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
WQPIAPVQAAERRVGVLGLGMLGRAVLESLRPFGLPLSGWSRAPRAIDSVRCLSGGEGLE
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHH
TLLATTDILVCMLPLTEETRGFLNAELFARLPAGAALAHVGRGAQLDHQALVAALDAGHL
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCC
SGAVVDVTDPEPLPPGHAFWNHPKILLTPHIASVTQAEPAAAAVIGNIKRHRAGLDPIGL
CCEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
VDRGRGY
CCCCCCC
>Mature Secondary Structure 
TFLFNSDAKRGAIFAEAFARELPDIAFAMDPESVEPEAVRYLITWTVPDDLARYRNLEI
EEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEEECCHHHHHHCCCEE
LFSIGAGIDQFRIDAVPAPVKIVRMVEDSIVKMMQEYATLAVLALHRDLPAYLDQQRRGI
EEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
WQPIAPVQAAERRVGVLGLGMLGRAVLESLRPFGLPLSGWSRAPRAIDSVRCLSGGEGLE
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHH
TLLATTDILVCMLPLTEETRGFLNAELFARLPAGAALAHVGRGAQLDHQALVAALDAGHL
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCC
SGAVVDVTDPEPLPPGHAFWNHPKILLTPHIASVTQAEPAAAAVIGNIKRHRAGLDPIGL
CCEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
VDRGRGY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA