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The map label for this gene is lutR [H]

Identifier: 86360965

GI number: 86360965

Start: 250775

End: 251488

Strand: Direct

Name: lutR [H]

Synonym: RHE_PF00235

Alternate gene names: 86360965

Gene position: 250775-251488 (Clockwise)

Preceding gene: 86360958

Following gene: 86360966

Centisome position: 39.03

GC content: 60.78

Gene sequence:

>714_bases
ATGACGTTTGCAGTCGATGCCGTTTCAAACCGGGGCGCAACGCGCTTCAGCGACATCATCTACGAGAAGATCGTGGGGAT
GATCGCGGACGGCAATTTTCCTGTCAACGAGCGGCTGCCGTCGGAGACCAAGCTTGCAGCCGATTTTGGCGCATCGAGAC
CCGTTGTGCGAGAAGCGCTCGAACGACTGAGAGCCGATGGCCTCGTCGTTTCCCGCAAGGGCTCTGGTTCCTATGTCCGG
CAGCGGCCGGATTCGTCGATGCTGAAGATGGTCCCGGTCGGCTCGCTTGCCGATGTCCAGCGGTTCTTCGAATTCCGCGC
CGGTCTCGAGGCCGAAGCTGCGGAGCTTGCCGCGCGAAACTGGCAGCCGGCTGACAAGGAGCGGATCACTGAGGCGCTTC
TTGCGATCGAACAATGTCTGAGGAACGGCGAACTCGGCGCCGACGAAGACCAGGCCCTGCACGACGCGATCGCGATGGCG
ACAGGCAATCAGTTTCACATCACCGTGCGCGAATGGTTCAAGCCGCATTTCGCCATCGGCCATTCCGTAACGCGAAGCCT
GAGCCTGAAACGGACTCCGGAGCAGATCCGCAGCGTTCAGGACGAGCATGCGGTCATCGTCGAGGCGATCTTCGCGCGGC
GGGAAGCCGAAGCGCATGATGCGATGAAGAAACACATACTGAATGCGCGTGCCCGCATGTTTCAGGGGGTTTGA

Upstream 100 bases:

>100_bases
CATCCCGGCGCATGGTCGAGAAGGCATATCCGGCTTCTGCTCGCCGGACGAAGAGCCGACGGGGCGAATTCGCCACTTGG
CGGGGGAGAAAAGAGCGATA

Downstream 100 bases:

>100_bases
GCGACAATGGGAGGAAGGACGATGAGACGGATTGCCATGACGGGTGCCGCCGGACGTGTCGGGACGCTGCTGCGCCCGTT
TCTGCGGCAGCATTTTGAGC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTFAVDAVSNRGATRFSDIIYEKIVGMIADGNFPVNERLPSETKLAADFGASRPVVREALERLRADGLVVSRKGSGSYVR
QRPDSSMLKMVPVGSLADVQRFFEFRAGLEAEAAELAARNWQPADKERITEALLAIEQCLRNGELGADEDQALHDAIAMA
TGNQFHITVREWFKPHFAIGHSVTRSLSLKRTPEQIRSVQDEHAVIVEAIFARREAEAHDAMKKHILNARARMFQGV

Sequences:

>Translated_237_residues
MTFAVDAVSNRGATRFSDIIYEKIVGMIADGNFPVNERLPSETKLAADFGASRPVVREALERLRADGLVVSRKGSGSYVR
QRPDSSMLKMVPVGSLADVQRFFEFRAGLEAEAAELAARNWQPADKERITEALLAIEQCLRNGELGADEDQALHDAIAMA
TGNQFHITVREWFKPHFAIGHSVTRSLSLKRTPEQIRSVQDEHAVIVEAIFARREAEAHDAMKKHILNARARMFQGV
>Mature_236_residues
TFAVDAVSNRGATRFSDIIYEKIVGMIADGNFPVNERLPSETKLAADFGASRPVVREALERLRADGLVVSRKGSGSYVRQ
RPDSSMLKMVPVGSLADVQRFFEFRAGLEAEAAELAARNWQPADKERITEALLAIEQCLRNGELGADEDQALHDAIAMAT
GNQFHITVREWFKPHFAIGHSVTRSLSLKRTPEQIRSVQDEHAVIVEAIFARREAEAHDAMKKHILNARARMFQGV

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=230, Percent_Identity=30.8695652173913, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1790032, Length=222, Percent_Identity=30.6306306306306, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1789353, Length=226, Percent_Identity=30.9734513274336, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790780, Length=234, Percent_Identity=29.4871794871795, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26369; Mature: 26238

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFAVDAVSNRGATRFSDIIYEKIVGMIADGNFPVNERLPSETKLAADFGASRPVVREAL
CCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHH
ERLRADGLVVSRKGSGSYVRQRPDSSMLKMVPVGSLADVQRFFEFRAGLEAEAAELAARN
HHHHHCCEEEEECCCCCHHHCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
WQPADKERITEALLAIEQCLRNGELGADEDQALHDAIAMATGNQFHITVREWFKPHFAIG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHH
HSVTRSLSLKRTPEQIRSVQDEHAVIVEAIFARREAEAHDAMKKHILNARARMFQGV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFAVDAVSNRGATRFSDIIYEKIVGMIADGNFPVNERLPSETKLAADFGASRPVVREAL
CEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHH
ERLRADGLVVSRKGSGSYVRQRPDSSMLKMVPVGSLADVQRFFEFRAGLEAEAAELAARN
HHHHHCCEEEEECCCCCHHHCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
WQPADKERITEALLAIEQCLRNGELGADEDQALHDAIAMATGNQFHITVREWFKPHFAIG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHH
HSVTRSLSLKRTPEQIRSVQDEHAVIVEAIFARREAEAHDAMKKHILNARARMFQGV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]