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The map label for this gene is siaP [H]

Identifier: 86360801

GI number: 86360801

Start: 67868

End: 68842

Strand: Direct

Name: siaP [H]

Synonym: RHE_PF00068

Alternate gene names: 86360801

Gene position: 67868-68842 (Clockwise)

Preceding gene: 86360800

Following gene: 86360802

Centisome position: 10.56

GC content: 58.97

Gene sequence:

>975_bases
ATGAAGAGCAGACTGGCAATGATACTGTGCACCGCAGCCGCGATCATGGCGACGACGGCGGTCGCCCATGCCCAGACCGT
CCTGAAATGGGCGCATGTCTACGAAACCTCGGAGCCGTTCCACACGGATTCCGTCTGGGCTGCCGAAGAGATCGCCAAGC
GCACCGACGGGCGCTACAAGATCGAGGTCTATCCGGCCTCGCAGCTCGGCAAGGAAGCCGACATCAACCAGGGCCTCAAG
CTCGGCACGGTCGACATCATCATCTCCGGGTCAAGCTTTGCCGCACGCGACTACAAGCCGATCGGCGTCACCTATTTTCC
CTATATCTTCCGTAACCCGGACCACCTGATCGCCTACACCAAGAGCGACGTCTTCAAGCGGCTTGCCAAGGGTTATGAGG
ACAAGATCGGCAACCACATCGCCGCCGTCAGCTATTACGGCACGCGCCACACCACCTCGAACAAGCCGATCGCCAAATGC
GCCGATATGCAGGGCCTGAAGATCCGCGTGCCTGATGTTCCCGCCTATCTCGCCATGCCGCGCGCCTGCGGCGCCAACAC
GACGCCGATCGCCTTTGCTGAAGTCTATCTCGCCTTGCAGAATGGCACGGTCGAGGCGCAGGAAAATCCGCTGACGACGA
TCGAGGCGAAGAAGTTCTATGAGGTCCAGAAGAACATCATCCTCACCGGCCACATCGTCGATCACCTGAACACCGTGATC
TCGAAGACGCGGTGGGCGAGCCTTTCCGACGAAGACAAGAAGATCTTCGGCGAAGTGATGCAGCAGGCCGCCGAGCGCAC
CACCAAGACGATCGCCGGCAAGGAAAACAGCCTCGTCGCCACCTTTAAGGAAAAGGGCCTCAACGTTACCGAGGTCGACA
AGAGCGATTTCGAGAAGAACGTGATGGAAAAGGTGAAGTTCGAGGACTTCGGCTATGAGAAGGCCGATTGGGAAGCGATC
CGGGCGATCCAGTAA

Upstream 100 bases:

>100_bases
CTTCGGGGAGGAGTTTTCGGGAGAGGAGGAGCGCGGCCGCGACAAGGCGGCTGCGAAAGGAGCCACCAAGACTTAAACCC
GAGCTCGGGAGGAGAGAGCA

Downstream 100 bases:

>100_bases
GCGCCTTGGGCGCGACGTCCGCCAACTCTTCCTTCGTCATGCTCGGGCTTGTCCCGAGCATCTGCCTCAGATCGATGGGG
CAGCAGATCCTCGGCACAAG

Product: C4-dicarboxylate ABC transporter, substrate-binding protein

Products: NA

Alternate protein names: Extracytoplasmic solute receptor protein siaP; N-acetylneuraminic-binding protein; Neu5Ac-binding protein [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKSRLAMILCTAAAIMATTAVAHAQTVLKWAHVYETSEPFHTDSVWAAEEIAKRTDGRYKIEVYPASQLGKEADINQGLK
LGTVDIIISGSSFAARDYKPIGVTYFPYIFRNPDHLIAYTKSDVFKRLAKGYEDKIGNHIAAVSYYGTRHTTSNKPIAKC
ADMQGLKIRVPDVPAYLAMPRACGANTTPIAFAEVYLALQNGTVEAQENPLTTIEAKKFYEVQKNIILTGHIVDHLNTVI
SKTRWASLSDEDKKIFGEVMQQAAERTTKTIAGKENSLVATFKEKGLNVTEVDKSDFEKNVMEKVKFEDFGYEKADWEAI
RAIQ

Sequences:

>Translated_324_residues
MKSRLAMILCTAAAIMATTAVAHAQTVLKWAHVYETSEPFHTDSVWAAEEIAKRTDGRYKIEVYPASQLGKEADINQGLK
LGTVDIIISGSSFAARDYKPIGVTYFPYIFRNPDHLIAYTKSDVFKRLAKGYEDKIGNHIAAVSYYGTRHTTSNKPIAKC
ADMQGLKIRVPDVPAYLAMPRACGANTTPIAFAEVYLALQNGTVEAQENPLTTIEAKKFYEVQKNIILTGHIVDHLNTVI
SKTRWASLSDEDKKIFGEVMQQAAERTTKTIAGKENSLVATFKEKGLNVTEVDKSDFEKNVMEKVKFEDFGYEKADWEAI
RAIQ
>Mature_324_residues
MKSRLAMILCTAAAIMATTAVAHAQTVLKWAHVYETSEPFHTDSVWAAEEIAKRTDGRYKIEVYPASQLGKEADINQGLK
LGTVDIIISGSSFAARDYKPIGVTYFPYIFRNPDHLIAYTKSDVFKRLAKGYEDKIGNHIAAVSYYGTRHTTSNKPIAKC
ADMQGLKIRVPDVPAYLAMPRACGANTTPIAFAEVYLALQNGTVEAQENPLTTIEAKKFYEVQKNIILTGHIVDHLNTVI
SKTRWASLSDEDKKIFGEVMQQAAERTTKTIAGKENSLVATFKEKGLNVTEVDKSDFEKNVMEKVKFEDFGYEKADWEAI
RAIQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system siaPT involved in the uptake of sialic acid. This protein specifically binds sialic acid [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=315, Percent_Identity=30.4761904761905, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36099; Mature: 36099

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRLAMILCTAAAIMATTAVAHAQTVLKWAHVYETSEPFHTDSVWAAEEIAKRTDGRYK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEE
IEVYPASQLGKEADINQGLKLGTVDIIISGSSFAARDYKPIGVTYFPYIFRNPDHLIAYT
EEEEEHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEECHHEECCCCCEEEEC
KSDVFKRLAKGYEDKIGNHIAAVSYYGTRHTTSNKPIAKCADMQGLKIRVPDVPAYLAMP
HHHHHHHHHCCHHHHHCCEEEEEEEECCCCCCCCCCHHHHCCCCCCEEECCCCCHHHHCC
RACGANTTPIAFAEVYLALQNGTVEAQENPLTTIEAKKFYEVQKNIILTGHIVDHLNTVI
CCCCCCCCCHHHEEEEEEEECCEEECCCCCCEEHHHHHHHHHHHCEEEEHHHHHHHHHHH
SKTRWASLSDEDKKIFGEVMQQAAERTTKTIAGKENSLVATFKEKGLNVTEVDKSDFEKN
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCEEECCHHHHHHH
VMEKVKFEDFGYEKADWEAIRAIQ
HHHHHHHHHCCCCCCCHHHHHCCC
>Mature Secondary Structure
MKSRLAMILCTAAAIMATTAVAHAQTVLKWAHVYETSEPFHTDSVWAAEEIAKRTDGRYK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEE
IEVYPASQLGKEADINQGLKLGTVDIIISGSSFAARDYKPIGVTYFPYIFRNPDHLIAYT
EEEEEHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEECHHEECCCCCEEEEC
KSDVFKRLAKGYEDKIGNHIAAVSYYGTRHTTSNKPIAKCADMQGLKIRVPDVPAYLAMP
HHHHHHHHHCCHHHHHCCEEEEEEEECCCCCCCCCCHHHHCCCCCCEEECCCCCHHHHCC
RACGANTTPIAFAEVYLALQNGTVEAQENPLTTIEAKKFYEVQKNIILTGHIVDHLNTVI
CCCCCCCCCHHHEEEEEEEECCEEECCCCCCEEHHHHHHHHHHHCEEEEHHHHHHHHHHH
SKTRWASLSDEDKKIFGEVMQQAAERTTKTIAGKENSLVATFKEKGLNVTEVDKSDFEKN
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCEEECCHHHHHHH
VMEKVKFEDFGYEKADWEAIRAIQ
HHHHHHHHHCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA