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The map label for this gene is mexA [H]

Identifier: 85860990

GI number: 85860990

Start: 3148364

End: 3149695

Strand: Direct

Name: mexA [H]

Synonym: SYN_02075

Alternate gene names: 85860990

Gene position: 3148364-3149695 (Clockwise)

Preceding gene: 85860988

Following gene: 85860991

Centisome position: 99.03

GC content: 58.26

Gene sequence:

>1332_bases
ATGGTTATTCAGGATCACAAGGCGTTTTTTTCTTCCGACATTGCGGATGAACAGCACAAAAATCTCCTGAACCGCACAGC
TGCGGGGCAGGCAATTTCATTCAGGTCGCAGAGGTGGCGTATGCAGATTCACAACGGCATGAGGTTCATGACTATTCTGG
CGGTCCTTGCCGGATTTCTTCTAACGGCAGGCTGCGGACAGAAAGCAGACAACGGCAAAGCAGGTCCGGGCGGACCTCCC
GAAGTCGCCGTAGTGACGGTACAGCCGGAGCGGGTCGTCATCACGACCGCGCTGTCGGGTCGAACCTCACCCTATCTCAT
CGCCGAGGTACGGCCTCAGGTCAGCGGCATTCTTCAGAAGCGATTTTTCAAGGAAGGCGCGGATGTCAAGGCCGGAGACG
TACTCTATCAGATCGATCCGGCCACCTATTCTGCAGCTTACGCCAGCGCCAGAGCGCATCTTGCACGAGCGGAAGCGAAT
GTGACGTCCATCCGCTACAGGGCTGACCGTTACGGGAAACTGGTCGCCATCAAGGCGGTCAGCCAGCAGGATTACGACGA
TGCCGCCGCCGCTCTCAAACAGGCCGAGGCGGAAATCCAGGCAGGCAAAGCGGCGGTGGAGGCCGCCCGGATCAATCTGG
CCTATACCCGTATATCCGCGCCCATTTCCGGGCGCATCGGCAGATCCCTGGTGACCGTAGGGGCGCTGGTGACTGCGGGG
CAGGGAATCGCACTGACCACGATCCAGCAGATCGACCCCATCTACGTCGACGTCACCCAGTCGAGCGCCAGCCTGCTGCG
TCTGCAGAAGAGCATGGCCAGCGGCGCGCTCAAAAAAGACGGATCTTCTTCAGCAAAAGTCAAGCTCCTTCTGGAAGACG
GAACCCCCTACCCCCTGGAAGGAACCATGCAATTCCGTGATATTACGGTAGACCCCACAAGCGGATCCTTTATCCTGCGC
ATTGTTTTTCCCAATCCGCAGGAAATTCTGCTGCCGGGTATGTATGTCCGGGCCATAATCGAGGAAGGCGTCAACGAACA
GGCGCTCCTGGTTCCCCAGCAGGGTGTCAGCCGCGACCCGAAGGGGAATCCTCTGGCTTTGATCGTAGATGCGGAAGGGA
AGGTTCAGCAGCGGATGTTGACCTGCGATCGGACTATCGGAGACAAATGGCTTGTTTCTTCAGGTCTTGCAGCTGGAGAT
CGGGTGATCGTCGAGGGAATGCAAAAGGTTAAACCGGGGGCCCCCGCAAAGGCTGTTCCTTTTGATGCCGCCAACATGCA
GAAGAGTGCTGCCCCCGGAAGCAGTGCCTCACCGGCTGCCGGAACGAACTGA

Upstream 100 bases:

>100_bases
GGGTTTTGTCGTTCTGGACGAGACCTTGATCACACAAACCGTCGAAGCCTGCTGGGACGGCATCAGAAGATGACAGGAAA
AACAGGGCATTAAATAATGA

Downstream 100 bases:

>100_bases
CGGAGGGCGTGATGTTATCAAGATTCTTCCTGGATCGGCCTGTTTTCGCCTGGGTCATTGCCATTATCATGATGGCGGCC
GGCGCGCTGGCGATCTACAA

Product: acriflavin resistance periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MVIQDHKAFFSSDIADEQHKNLLNRTAAGQAISFRSQRWRMQIHNGMRFMTILAVLAGFLLTAGCGQKADNGKAGPGGPP
EVAVVTVQPERVVITTALSGRTSPYLIAEVRPQVSGILQKRFFKEGADVKAGDVLYQIDPATYSAAYASARAHLARAEAN
VTSIRYRADRYGKLVAIKAVSQQDYDDAAAALKQAEAEIQAGKAAVEAARINLAYTRISAPISGRIGRSLVTVGALVTAG
QGIALTTIQQIDPIYVDVTQSSASLLRLQKSMASGALKKDGSSSAKVKLLLEDGTPYPLEGTMQFRDITVDPTSGSFILR
IVFPNPQEILLPGMYVRAIIEEGVNEQALLVPQQGVSRDPKGNPLALIVDAEGKVQQRMLTCDRTIGDKWLVSSGLAAGD
RVIVEGMQKVKPGAPAKAVPFDAANMQKSAAPGSSASPAAGTN

Sequences:

>Translated_443_residues
MVIQDHKAFFSSDIADEQHKNLLNRTAAGQAISFRSQRWRMQIHNGMRFMTILAVLAGFLLTAGCGQKADNGKAGPGGPP
EVAVVTVQPERVVITTALSGRTSPYLIAEVRPQVSGILQKRFFKEGADVKAGDVLYQIDPATYSAAYASARAHLARAEAN
VTSIRYRADRYGKLVAIKAVSQQDYDDAAAALKQAEAEIQAGKAAVEAARINLAYTRISAPISGRIGRSLVTVGALVTAG
QGIALTTIQQIDPIYVDVTQSSASLLRLQKSMASGALKKDGSSSAKVKLLLEDGTPYPLEGTMQFRDITVDPTSGSFILR
IVFPNPQEILLPGMYVRAIIEEGVNEQALLVPQQGVSRDPKGNPLALIVDAEGKVQQRMLTCDRTIGDKWLVSSGLAAGD
RVIVEGMQKVKPGAPAKAVPFDAANMQKSAAPGSSASPAAGTN
>Mature_443_residues
MVIQDHKAFFSSDIADEQHKNLLNRTAAGQAISFRSQRWRMQIHNGMRFMTILAVLAGFLLTAGCGQKADNGKAGPGGPP
EVAVVTVQPERVVITTALSGRTSPYLIAEVRPQVSGILQKRFFKEGADVKAGDVLYQIDPATYSAAYASARAHLARAEAN
VTSIRYRADRYGKLVAIKAVSQQDYDDAAAALKQAEAEIQAGKAAVEAARINLAYTRISAPISGRIGRSLVTVGALVTAG
QGIALTTIQQIDPIYVDVTQSSASLLRLQKSMASGALKKDGSSSAKVKLLLEDGTPYPLEGTMQFRDITVDPTSGSFILR
IVFPNPQEILLPGMYVRAIIEEGVNEQALLVPQQGVSRDPKGNPLALIVDAEGKVQQRMLTCDRTIGDKWLVSSGLAAGD
RVIVEGMQKVKPGAPAKAVPFDAANMQKSAAPGSSASPAAGTN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=393, Percent_Identity=52.9262086513995, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1789665, Length=380, Percent_Identity=52.3684210526316, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1789929, Length=381, Percent_Identity=47.244094488189, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI87082036, Length=319, Percent_Identity=28.5266457680251, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87081796, Length=354, Percent_Identity=28.8135593220339, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081951, Length=132, Percent_Identity=30.3030303030303, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 47023; Mature: 47023

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIQDHKAFFSSDIADEQHKNLLNRTAAGQAISFRSQRWRMQIHNGMRFMTILAVLAGFL
CEECCCHHHHHHCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LTAGCGQKADNGKAGPGGPPEVAVVTVQPERVVITTALSGRTSPYLIAEVRPQVSGILQK
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCEEEEECCHHHHHHHHH
RFFKEGADVKAGDVLYQIDPATYSAAYASARAHLARAEANVTSIRYRADRYGKLVAIKAV
HHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEC
SQQDYDDAAAALKQAEAEIQAGKAAVEAARINLAYTRISAPISGRIGRSLVTVGALVTAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHCC
QGIALTTIQQIDPIYVDVTQSSASLLRLQKSMASGALKKDGSSSAKVKLLLEDGTPYPLE
CCEEEEEHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCC
GTMQFRDITVDPTSGSFILRIVFPNPQEILLPGMYVRAIIEEGVNEQALLVPQQGVSRDP
CCEEEEEEEECCCCCCEEEEEECCCCHHEECCHHHHHHHHHHCCCCCEEEECCCCCCCCC
KGNPLALIVDAEGKVQQRMLTCDRTIGDKWLVSSGLAAGDRVIVEGMQKVKPGAPAKAVP
CCCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCC
FDAANMQKSAAPGSSASPAAGTN
CCHHCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVIQDHKAFFSSDIADEQHKNLLNRTAAGQAISFRSQRWRMQIHNGMRFMTILAVLAGFL
CEECCCHHHHHHCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LTAGCGQKADNGKAGPGGPPEVAVVTVQPERVVITTALSGRTSPYLIAEVRPQVSGILQK
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCEEEEECCHHHHHHHHH
RFFKEGADVKAGDVLYQIDPATYSAAYASARAHLARAEANVTSIRYRADRYGKLVAIKAV
HHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEC
SQQDYDDAAAALKQAEAEIQAGKAAVEAARINLAYTRISAPISGRIGRSLVTVGALVTAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHCC
QGIALTTIQQIDPIYVDVTQSSASLLRLQKSMASGALKKDGSSSAKVKLLLEDGTPYPLE
CCEEEEEHHHCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCC
GTMQFRDITVDPTSGSFILRIVFPNPQEILLPGMYVRAIIEEGVNEQALLVPQQGVSRDP
CCEEEEEEEECCCCCCEEEEEECCCCHHEECCHHHHHHHHHHCCCCCEEEECCCCCCCCC
KGNPLALIVDAEGKVQQRMLTCDRTIGDKWLVSSGLAAGDRVIVEGMQKVKPGAPAKAVP
CCCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCC
FDAANMQKSAAPGSSASPAAGTN
CCHHCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]