Click here to switch to the map view.

The map label for this gene is pckA [H]

Identifier: 85860979

GI number: 85860979

Start: 3137004

End: 3138653

Strand: Direct

Name: pckA [H]

Synonym: SYN_02086

Alternate gene names: 85860979

Gene position: 3137004-3138653 (Clockwise)

Preceding gene: 85860978

Following gene: 85860980

Centisome position: 98.67

GC content: 57.03

Gene sequence:

>1650_bases
ATGAATATATTCAAGGCAGCAGCAAAGCAGCTTTATGAAACCGCCCGACAGGAAGGCCGCCTTATCGAAGGCCGCCCGCT
GGAGGAGTTGAAGGAAATCGCCCTGCGGCAGGAGGGGGTGATTCAGACCCATATCGGCTCCGTGGCGGCGGATTCCGAGC
CCATGTCCCGGTCGGCGCCTCACACCCGCAACAGTGTGGATCACCCCTTCGGCGATGAGGAAGCTCAACTGGCCGACCAA
ATGGTGGAGTCGCTGAAAAAAGAGAGGCTGATTTCCCTTGATACCTACATCGGCGATGGTCGGGACGGCGTCTCCGCCCG
ATTTCTTGTCCCGGAACAGTATGCCCAGATCGCCTATGGTCTCAAACTCCTCTTCAATCATCCACCGCTCGTTCTGGACA
ACCCCACCTATACCATCATCTATTACACCGACGAAGCCTTTGAACGCAACCGTGCAAAAAAGCTGGTGGACAAGGACATC
TCCATCCGCCTCATGCTGGGCGACAAGCGTGGAGATCAGGTTAAAATCTGCCGCAATACGATTTATCTCGGAGAAGGGAA
AAAAGGAATTTTCCAGTTCGAGGACTGGAGGATCAAGGCCATCGACAAAACCGGAATTTTTCTCCATGCCGGCGCGCGCC
GAGATTCGCTCTGGGTGTATGATCAGGAAACCGCCCGGATGGAACTGACGGATATCGTGACCGCCGTGGCCGGACTGACC
GCGACCGGAAAGACCACGACTCTCTGCCGCAAATTCACGAAAGTTCCGCTTGAGCGTTCGGAGATGATCGGCGACGACGG
GGGCGCACTGGGACTGGACGCCAGCTACGCGGCCTTCGAGATGGGCGGAGTTTATGTGAAAACGGAAGGACTGGACGCCA
GCCAGCCGGAAATCCTCCGGGCTGCGGAATCCAGGGACGCCTTCCTGGAAAATGTCGCTCTGACCCAGTACCCCTATATG
CCTAATTTCGCGGATATTTCCAAGACGGGAAACGGCAGGGCCGTAGTCTCGCGGGACAACCTGGAAATCGCCTCGAAAAG
CCTGCGGGCGGACCGCATCCACTACATCATCATCCTGACCCGGAATCCCCTGGTCAATGTGCTCAGCCGCCTGACGCCGG
AGCAGGCCACCATGCAGTTCATCTACGGCGAATCCATTGAAAGCAGCGGCGGCAACCCCGAAGAGGCCGGCCGATTCAAA
CGGGAATTTTTCCTCGATCCCTTCATGGCGGGCGACCGCCTGGAACATGCCATGATTTTTTACGATATCGTCAAGCGGAA
CAAAATCCAGTGCTATCTGGCCAATACGGGAACCATCGGTCTGAAGGAGGAAAAGGTCACCCTGCGCCAGTCCCTCGCGG
CCTACAACGATCTGGTCCGCATGCAGCTCCGCTTCAGCCTCGAGGCCGACCACCTGGGCTATCACTATCCCATCAAGTGC
GACCGGGCCAATCTGGACCTCATGACCGCTTACCGGCTTTTTGACGACCCCGTTATCCTGGAAAAACGGCTGCAGGATTT
TTACCGGGGCCGTCGGGAATACCTGGAGGAATTTGAAGGACGATACGGCCGGATCCCCGAACCGATCCGCGAGTCTCTGC
CTTATCACTACAAGGAATTTTCCCCCCGGGAAGCCCTGGACATTGAATAG

Upstream 100 bases:

>100_bases
ATTGAACAGGCAGCAGAATCCGCAGGCCATAAAAATCTGTGGTCATCCTGCCGGATTCTGAAAAGGAAGTCGTGAAACTC
AGCCCAAGGGGAGAAGACAG

Downstream 100 bases:

>100_bases
TCGGAACAAGCCGATCCTGTCATGCCCCGTTATTGCGCATCCGGCCCCGGGCCAGCGAGGCAAAAATTCCCAGAAAACAC
AGGATGGACAGAATCGAAAA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MNIFKAAAKQLYETARQEGRLIEGRPLEELKEIALRQEGVIQTHIGSVAADSEPMSRSAPHTRNSVDHPFGDEEAQLADQ
MVESLKKERLISLDTYIGDGRDGVSARFLVPEQYAQIAYGLKLLFNHPPLVLDNPTYTIIYYTDEAFERNRAKKLVDKDI
SIRLMLGDKRGDQVKICRNTIYLGEGKKGIFQFEDWRIKAIDKTGIFLHAGARRDSLWVYDQETARMELTDIVTAVAGLT
ATGKTTTLCRKFTKVPLERSEMIGDDGGALGLDASYAAFEMGGVYVKTEGLDASQPEILRAAESRDAFLENVALTQYPYM
PNFADISKTGNGRAVVSRDNLEIASKSLRADRIHYIIILTRNPLVNVLSRLTPEQATMQFIYGESIESSGGNPEEAGRFK
REFFLDPFMAGDRLEHAMIFYDIVKRNKIQCYLANTGTIGLKEEKVTLRQSLAAYNDLVRMQLRFSLEADHLGYHYPIKC
DRANLDLMTAYRLFDDPVILEKRLQDFYRGRREYLEEFEGRYGRIPEPIRESLPYHYKEFSPREALDIE

Sequences:

>Translated_549_residues
MNIFKAAAKQLYETARQEGRLIEGRPLEELKEIALRQEGVIQTHIGSVAADSEPMSRSAPHTRNSVDHPFGDEEAQLADQ
MVESLKKERLISLDTYIGDGRDGVSARFLVPEQYAQIAYGLKLLFNHPPLVLDNPTYTIIYYTDEAFERNRAKKLVDKDI
SIRLMLGDKRGDQVKICRNTIYLGEGKKGIFQFEDWRIKAIDKTGIFLHAGARRDSLWVYDQETARMELTDIVTAVAGLT
ATGKTTTLCRKFTKVPLERSEMIGDDGGALGLDASYAAFEMGGVYVKTEGLDASQPEILRAAESRDAFLENVALTQYPYM
PNFADISKTGNGRAVVSRDNLEIASKSLRADRIHYIIILTRNPLVNVLSRLTPEQATMQFIYGESIESSGGNPEEAGRFK
REFFLDPFMAGDRLEHAMIFYDIVKRNKIQCYLANTGTIGLKEEKVTLRQSLAAYNDLVRMQLRFSLEADHLGYHYPIKC
DRANLDLMTAYRLFDDPVILEKRLQDFYRGRREYLEEFEGRYGRIPEPIRESLPYHYKEFSPREALDIE
>Mature_549_residues
MNIFKAAAKQLYETARQEGRLIEGRPLEELKEIALRQEGVIQTHIGSVAADSEPMSRSAPHTRNSVDHPFGDEEAQLADQ
MVESLKKERLISLDTYIGDGRDGVSARFLVPEQYAQIAYGLKLLFNHPPLVLDNPTYTIIYYTDEAFERNRAKKLVDKDI
SIRLMLGDKRGDQVKICRNTIYLGEGKKGIFQFEDWRIKAIDKTGIFLHAGARRDSLWVYDQETARMELTDIVTAVAGLT
ATGKTTTLCRKFTKVPLERSEMIGDDGGALGLDASYAAFEMGGVYVKTEGLDASQPEILRAAESRDAFLENVALTQYPYM
PNFADISKTGNGRAVVSRDNLEIASKSLRADRIHYIIILTRNPLVNVLSRLTPEQATMQFIYGESIESSGGNPEEAGRFK
REFFLDPFMAGDRLEHAMIFYDIVKRNKIQCYLANTGTIGLKEEKVTLRQSLAAYNDLVRMQLRFSLEADHLGYHYPIKC
DRANLDLMTAYRLFDDPVILEKRLQDFYRGRREYLEEFEGRYGRIPEPIRESLPYHYKEFSPREALDIE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322950, Length=272, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 62527; Mature: 62527

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFKAAAKQLYETARQEGRLIEGRPLEELKEIALRQEGVIQTHIGSVAADSEPMSRSAP
CCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCC
HTRNSVDHPFGDEEAQLADQMVESLKKERLISLDTYIGDGRDGVSARFLVPEQYAQIAYG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEHEECCCCCCCEEEEECHHHHHHHHHH
LKLLFNHPPLVLDNPTYTIIYYTDEAFERNRAKKLVDKDISIRLMLGDKRGDQVKICRNT
HHHEECCCCEEECCCCEEEEEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEE
IYLGEGKKGIFQFEDWRIKAIDKTGIFLHAGARRDSLWVYDQETARMELTDIVTAVAGLT
EEECCCCCCCEEECCCEEEEEECCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHCCC
ATGKTTTLCRKFTKVPLERSEMIGDDGGALGLDASYAAFEMGGVYVKTEGLDASQPEILR
CCCCHHHHHHHHHHCCCCHHHHCCCCCCEECCCCCHHEEEECCEEEEECCCCCCCCHHHH
AAESRDAFLENVALTQYPYMPNFADISKTGNGRAVVSRDNLEIASKSLRADRIHYIIILT
HHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEECCCHHHHHHHHCCCCEEEEEEEC
RNPLVNVLSRLTPEQATMQFIYGESIESSGGNPEEAGRFKREFFLDPFMAGDRLEHAMIF
CCHHHHHHHHCCCCHHHEEEHCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHH
YDIVKRNKIQCYLANTGTIGLKEEKVTLRQSLAAYNDLVRMQLRFSLEADHLGYHYPIKC
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEE
DRANLDLMTAYRLFDDPVILEKRLQDFYRGRREYLEEFEGRYGRIPEPIRESLPYHYKEF
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHCC
SPREALDIE
CCCCCCCCC
>Mature Secondary Structure
MNIFKAAAKQLYETARQEGRLIEGRPLEELKEIALRQEGVIQTHIGSVAADSEPMSRSAP
CCHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCC
HTRNSVDHPFGDEEAQLADQMVESLKKERLISLDTYIGDGRDGVSARFLVPEQYAQIAYG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEHEECCCCCCCEEEEECHHHHHHHHHH
LKLLFNHPPLVLDNPTYTIIYYTDEAFERNRAKKLVDKDISIRLMLGDKRGDQVKICRNT
HHHEECCCCEEECCCCEEEEEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEE
IYLGEGKKGIFQFEDWRIKAIDKTGIFLHAGARRDSLWVYDQETARMELTDIVTAVAGLT
EEECCCCCCCEEECCCEEEEEECCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHCCC
ATGKTTTLCRKFTKVPLERSEMIGDDGGALGLDASYAAFEMGGVYVKTEGLDASQPEILR
CCCCHHHHHHHHHHCCCCHHHHCCCCCCEECCCCCHHEEEECCEEEEECCCCCCCCHHHH
AAESRDAFLENVALTQYPYMPNFADISKTGNGRAVVSRDNLEIASKSLRADRIHYIIILT
HHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEECCCHHHHHHHHCCCCEEEEEEEC
RNPLVNVLSRLTPEQATMQFIYGESIESSGGNPEEAGRFKREFFLDPFMAGDRLEHAMIF
CCHHHHHHHHCCCCHHHEEEHCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHHH
YDIVKRNKIQCYLANTGTIGLKEEKVTLRQSLAAYNDLVRMQLRFSLEADHLGYHYPIKC
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEE
DRANLDLMTAYRLFDDPVILEKRLQDFYRGRREYLEEFEGRYGRIPEPIRESLPYHYKEF
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHCC
SPREALDIE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]