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The map label for this gene is birA [H]

Identifier: 85860938

GI number: 85860938

Start: 3106369

End: 3107370

Strand: Direct

Name: birA [H]

Synonym: SYN_00211

Alternate gene names: 85860938

Gene position: 3106369-3107370 (Clockwise)

Preceding gene: 85860935

Following gene: 85860939

Centisome position: 97.71

GC content: 57.49

Gene sequence:

>1002_bases
GTGGTATTCAAGGCCGCGATGAACACAAAGGAACAGTTGCTGCGATACCTCAAGAAAGAACAGGGACGGTTTGTATCCGG
AGAAAAGGTCAGTTCCAGGCTGGCAATTTCCCGCTCCGCGATATGGAAACAGATCAGCAGGCTGAAGGAAGAGGGGTACG
ACATCGAATCTTCCCCCCGAAGGGGGTATGCCCTGCGGAGCATCCCTGATTGTCTCCTGGCCGGCGAGATCCGGGAAGGC
CTGAACACCCGCCTGATCGGACGGGGTCCGATCTTTCATTTCCAGCAAACGGACAACACCAACAATCAGGGCAAGGCGCT
GGCTTATGAAGGCGCTCCGGAGGGCGCCCTGGTCATAGCCGAAGAGCAGTCCCAGGGACGGGGCCGTCGGGGAAGGACCT
GGTTTTCCCCTCACAGTCAGGGGATTTACGCAACCGTGATCCTGCGCCCTCTCATCCCCCTCAGCGAAACCCCGAAGCTC
ACCCTCCTTGCTGCCGTGGCGGCAACCGAGGCCCTGGAGACGCAAACCGGTCTGCCCATCCGGATCAAGTGGCCCAACGA
TATCCTGGCAAATGGTCGCAAACTGGCCGGAATCCTTACTGAAATCGGAACGGAAATGGATGCCGTGGATTTTGCGGTCA
TCGGAATGGGTCTGAACGTAAATGTCCCCCAGGCATCCTTCCCCTCCGATCTTCGATACCCGGCAACTTCCATACTGATC
GAAACAGGGCGGCCTTTTCCGCGAATCCGCCTTCTCCAGAGCTGGCTGGAGGCTCTGGAAAAATATTACAGTCTTTTGCT
CCAGGGCGATTTTGATAACATCCTTTCGCGCTGGAAGTTCCTGACGGATATTGTGGGAAGCCGGATCGCCGTCGAGATGC
CGGGACGCCGGTATATCGGCGAAGTGCAGGACGTGGATGGAAACGGGGTTCTGATCCTGAAGGAATCCGACGGGGCCCTT
CAGCGGATTTTTTCCGGAGATGTGACCCTCCTCACGGTCTGA

Upstream 100 bases:

>100_bases
CAAAATTTTCTCCTGTCGTGTAATGGAAGACATCGCCTATCAGAGGACAGGCTCCTTTGTCAACCGGCAAATGATAACGG
GTTGACAAAAAGAAACCTCG

Downstream 100 bases:

>100_bases
CGGCGGGATGTAAGAGGAACAAAATAATGAATAAATCAGGACGCACCGCATCCCCTCTGCTCGGGATGATCTATGCCTCT
CTTTTCGGAGCCATGACGGC

Product: biotin-(acetyl-CoA carboxylase) ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MVFKAAMNTKEQLLRYLKKEQGRFVSGEKVSSRLAISRSAIWKQISRLKEEGYDIESSPRRGYALRSIPDCLLAGEIREG
LNTRLIGRGPIFHFQQTDNTNNQGKALAYEGAPEGALVIAEEQSQGRGRRGRTWFSPHSQGIYATVILRPLIPLSETPKL
TLLAAVAATEALETQTGLPIRIKWPNDILANGRKLAGILTEIGTEMDAVDFAVIGMGLNVNVPQASFPSDLRYPATSILI
ETGRPFPRIRLLQSWLEALEKYYSLLLQGDFDNILSRWKFLTDIVGSRIAVEMPGRRYIGEVQDVDGNGVLILKESDGAL
QRIFSGDVTLLTV

Sequences:

>Translated_333_residues
MVFKAAMNTKEQLLRYLKKEQGRFVSGEKVSSRLAISRSAIWKQISRLKEEGYDIESSPRRGYALRSIPDCLLAGEIREG
LNTRLIGRGPIFHFQQTDNTNNQGKALAYEGAPEGALVIAEEQSQGRGRRGRTWFSPHSQGIYATVILRPLIPLSETPKL
TLLAAVAATEALETQTGLPIRIKWPNDILANGRKLAGILTEIGTEMDAVDFAVIGMGLNVNVPQASFPSDLRYPATSILI
ETGRPFPRIRLLQSWLEALEKYYSLLLQGDFDNILSRWKFLTDIVGSRIAVEMPGRRYIGEVQDVDGNGVLILKESDGAL
QRIFSGDVTLLTV
>Mature_333_residues
MVFKAAMNTKEQLLRYLKKEQGRFVSGEKVSSRLAISRSAIWKQISRLKEEGYDIESSPRRGYALRSIPDCLLAGEIREG
LNTRLIGRGPIFHFQQTDNTNNQGKALAYEGAPEGALVIAEEQSQGRGRRGRTWFSPHSQGIYATVILRPLIPLSETPKL
TLLAAVAATEALETQTGLPIRIKWPNDILANGRKLAGILTEIGTEMDAVDFAVIGMGLNVNVPQASFPSDLRYPATSILI
ETGRPFPRIRLLQSWLEALEKYYSLLLQGDFDNILSRWKFLTDIVGSRIAVEMPGRRYIGEVQDVDGNGVLILKESDGAL
QRIFSGDVTLLTV

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=311, Percent_Identity=29.903536977492, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36956; Mature: 36956

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFKAAMNTKEQLLRYLKKEQGRFVSGEKVSSRLAISRSAIWKQISRLKEEGYDIESSPR
CEEHHHCCCHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RGYALRSIPDCLLAGEIREGLNTRLIGRGPIFHFQQTDNTNNQGKALAYEGAPEGALVIA
CCCCHHHCHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCEEEECCCCCCEEEEE
EEQSQGRGRRGRTWFSPHSQGIYATVILRPLIPLSETPKLTLLAAVAATEALETQTGLPI
ECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEE
RIKWPNDILANGRKLAGILTEIGTEMDAVDFAVIGMGLNVNVPQASFPSDLRYPATSILI
EEECCCHHHHCCHHHHHHHHHHCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCEEEEE
ETGRPFPRIRLLQSWLEALEKYYSLLLQGDFDNILSRWKFLTDIVGSRIAVEMPGRRYIG
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHCC
EVQDVDGNGVLILKESDGALQRIFSGDVTLLTV
CEEECCCCCEEEEECCCCHHHHHHCCCEEEEEC
>Mature Secondary Structure
MVFKAAMNTKEQLLRYLKKEQGRFVSGEKVSSRLAISRSAIWKQISRLKEEGYDIESSPR
CEEHHHCCCHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RGYALRSIPDCLLAGEIREGLNTRLIGRGPIFHFQQTDNTNNQGKALAYEGAPEGALVIA
CCCCHHHCHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCCEEEECCCCCCEEEEE
EEQSQGRGRRGRTWFSPHSQGIYATVILRPLIPLSETPKLTLLAAVAATEALETQTGLPI
ECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHCCCCEE
RIKWPNDILANGRKLAGILTEIGTEMDAVDFAVIGMGLNVNVPQASFPSDLRYPATSILI
EEECCCHHHHCCHHHHHHHHHHCCCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCEEEEE
ETGRPFPRIRLLQSWLEALEKYYSLLLQGDFDNILSRWKFLTDIVGSRIAVEMPGRRYIG
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHCC
EVQDVDGNGVLILKESDGALQRIFSGDVTLLTV
CEEECCCCCEEEEECCCCHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]