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The map label for this gene is kinE [H]

Identifier: 85860889

GI number: 85860889

Start: 3055427

End: 3057034

Strand: Direct

Name: kinE [H]

Synonym: SYN_01636

Alternate gene names: 85860889

Gene position: 3055427-3057034 (Clockwise)

Preceding gene: 85860880

Following gene: 85860890

Centisome position: 96.1

GC content: 43.03

Gene sequence:

>1608_bases
TTGGAAATCTTCGAACAATTTCCTCTTGGGGTGTATGTCCATTTTTTTCAACATAAAGTACGCATAAACCGGATGAACCA
TAAAATGGAACTAAAAGATATCTTGCTGGCACATCGTAAAGAGATCAACGTAAAATGGAGTATTGTAGTCGATAAACACT
ACTGTGAGCAGGGGTATAGACAGGAACCGTTCGAAGATACGTTAGCTGTTAATCTGGAAGCTGTAGATGCAAACTATGCC
GTACTCGCAAACAGGGATTTCGCACCGTTAGATGATTTTATCGAGAAGATTGCCAGAAAGCGGTTTGCCGCCGGATTCGA
CCTTTCAGACGTTCAAATGGCCTTTGAATATTACCGGACAATTACCATCCCCATCATTACGAAAGAATTGCCGCAGAACA
AAATGACCCCAGCTCTGGAAAGGCTTAATAATTGTCTCTCCTATACGATCATCAAGTTCAGTGATTATTTTCAGAACCTC
CATGAAAAGGCACTCAAGGAGCATGCTGAACACCTCGAACTGGAGGTCATGAAGCGGACGAAAGAATTAGCGGAGTTGGA
GGCAAAATACCGGGTCCTCGTAGAGGAGATCAATGACGGCTATTTTGTAGAAAAGAAGGGGAGAATCATCTTTGCGAACC
ATGCTTTTTGCGATATGCACGGATATACCCCGACGGAGGTCATCAATCAGCCATACATCAATTTTGTGGCGTCTGAATAT
GTTTCCAAGGTAACAAGTTATTTCGAGAAGGACTTAAACAGGGAAAGCGAATCAGAACAATATGCCTATAAAAGACTCCA
TAAGGATGGCAGTTTTTTCCCAACTGAAAACAAGGTAAAGCGCATCTGGTATAAGGGAGACTATGCATGGGCAGGTATCT
GCCGGGATATCACGGAAAGAGTGAAAATGGCGGAACGCATCCGTGAATCGGAAAAACTCGCACACATTGGTCAGCTTACC
ACGTCTCTCGCCCATGAAATTAGGAATCCCCTCTCATCCATCAAGATGAGCATTCGAATGCTCCTGAAAAGCATGGACTT
TGATGGAGATAACAAAAAGACGCTGGAAATAGCAGACCGGGAGATTTCGCATTTAGAGAGAATCCTTACAGAAATGCTTG
ATTTTGCCAGGCCTGTCAAACTCTGTTTTGAACTTCTCTCAATCAATCAACTTATTTACTCATGCCTGGAAATTCTGAAA
GTCACGATCCGTGAAAAAGAGATCACCGTGATAGAGAAACTTTCTAAAAGAATGTCCCCATCGATGATGGATGGGAATAA
GATTGAACATGCCATTATCAACATCCTTCTCAATGCAATTGAAGTGCTGCCTACGGGAGGAATCATATCGATCGAAACAC
ATCATCGGAAATTGGATCACAAGCCCTTTATATCCGTCGAAATCTCAGACAACGGTCCGGGTGTGACGTCGGAAGATCTG
CCTTACATTTTCAACCCCTTCTTCAGTAAAAAAAAGAAAGGCACAGGTCTTGGCCTTTCAAATGTGAAAAAAGTCGTGGA
TGCCCATGGAGGGAAGATTGCCGCTTCGGCTACCTCAAAAGGTTTTTCCATTTCTTTTACGATCCCTGTAAGGAAGGATC
ATGTGTAA

Upstream 100 bases:

>100_bases
CGTGATACTTCTTCCTTCGTATCGCGTCCTAACATAATCGTAGCCGACGAGGCTGAATCCGTGGTAAAGAAAACTCGTGG
ACATGCTTCCCCTCCGTTAA

Downstream 100 bases:

>100_bases
GAAGAAAATTTTAATTATAGATGATGATGCCTCTCAACTGGAACTGCTCAATATATTTTTCAGTGGAAGAGGTTATGCCG
TTCATTGCTCCGCCACCGCG

Product: signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MEIFEQFPLGVYVHFFQHKVRINRMNHKMELKDILLAHRKEINVKWSIVVDKHYCEQGYRQEPFEDTLAVNLEAVDANYA
VLANRDFAPLDDFIEKIARKRFAAGFDLSDVQMAFEYYRTITIPIITKELPQNKMTPALERLNNCLSYTIIKFSDYFQNL
HEKALKEHAEHLELEVMKRTKELAELEAKYRVLVEEINDGYFVEKKGRIIFANHAFCDMHGYTPTEVINQPYINFVASEY
VSKVTSYFEKDLNRESESEQYAYKRLHKDGSFFPTENKVKRIWYKGDYAWAGICRDITERVKMAERIRESEKLAHIGQLT
TSLAHEIRNPLSSIKMSIRMLLKSMDFDGDNKKTLEIADREISHLERILTEMLDFARPVKLCFELLSINQLIYSCLEILK
VTIREKEITVIEKLSKRMSPSMMDGNKIEHAIINILLNAIEVLPTGGIISIETHHRKLDHKPFISVEISDNGPGVTSEDL
PYIFNPFFSKKKKGTGLGLSNVKKVVDAHGGKIAASATSKGFSISFTIPVRKDHV

Sequences:

>Translated_535_residues
MEIFEQFPLGVYVHFFQHKVRINRMNHKMELKDILLAHRKEINVKWSIVVDKHYCEQGYRQEPFEDTLAVNLEAVDANYA
VLANRDFAPLDDFIEKIARKRFAAGFDLSDVQMAFEYYRTITIPIITKELPQNKMTPALERLNNCLSYTIIKFSDYFQNL
HEKALKEHAEHLELEVMKRTKELAELEAKYRVLVEEINDGYFVEKKGRIIFANHAFCDMHGYTPTEVINQPYINFVASEY
VSKVTSYFEKDLNRESESEQYAYKRLHKDGSFFPTENKVKRIWYKGDYAWAGICRDITERVKMAERIRESEKLAHIGQLT
TSLAHEIRNPLSSIKMSIRMLLKSMDFDGDNKKTLEIADREISHLERILTEMLDFARPVKLCFELLSINQLIYSCLEILK
VTIREKEITVIEKLSKRMSPSMMDGNKIEHAIINILLNAIEVLPTGGIISIETHHRKLDHKPFISVEISDNGPGVTSEDL
PYIFNPFFSKKKKGTGLGLSNVKKVVDAHGGKIAASATSKGFSISFTIPVRKDHV
>Mature_535_residues
MEIFEQFPLGVYVHFFQHKVRINRMNHKMELKDILLAHRKEINVKWSIVVDKHYCEQGYRQEPFEDTLAVNLEAVDANYA
VLANRDFAPLDDFIEKIARKRFAAGFDLSDVQMAFEYYRTITIPIITKELPQNKMTPALERLNNCLSYTIIKFSDYFQNL
HEKALKEHAEHLELEVMKRTKELAELEAKYRVLVEEINDGYFVEKKGRIIFANHAFCDMHGYTPTEVINQPYINFVASEY
VSKVTSYFEKDLNRESESEQYAYKRLHKDGSFFPTENKVKRIWYKGDYAWAGICRDITERVKMAERIRESEKLAHIGQLT
TSLAHEIRNPLSSIKMSIRMLLKSMDFDGDNKKTLEIADREISHLERILTEMLDFARPVKLCFELLSINQLIYSCLEILK
VTIREKEITVIEKLSKRMSPSMMDGNKIEHAIINILLNAIEVLPTGGIISIETHHRKLDHKPFISVEISDNGPGVTSEDL
PYIFNPFFSKKKKGTGLGLSNVKKVVDAHGGKIAASATSKGFSISFTIPVRKDHV

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=26.890756302521, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1790436, Length=255, Percent_Identity=29.0196078431373, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1790300, Length=249, Percent_Identity=28.5140562248996, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87081816, Length=221, Percent_Identity=27.6018099547511, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI145693157, Length=264, Percent_Identity=25, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786600, Length=377, Percent_Identity=22.2811671087533, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790346, Length=247, Percent_Identity=24.6963562753036, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=245, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789808, Length=219, Percent_Identity=24.2009132420091, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786783, Length=241, Percent_Identity=24.4813278008299, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61898; Mature: 61898

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIFEQFPLGVYVHFFQHKVRINRMNHKMELKDILLAHRKEINVKWSIVVDKHYCEQGYR
CCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEEHHHHHCCCC
QEPFEDTLAVNLEAVDANYAVLANRDFAPLDDFIEKIARKRFAAGFDLSDVQMAFEYYRT
CCCCCCHHEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ITIPIITKELPQNKMTPALERLNNCLSYTIIKFSDYFQNLHEKALKEHAEHLELEVMKRT
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELAELEAKYRVLVEEINDGYFVEKKGRIIFANHAFCDMHGYTPTEVINQPYINFVASEY
HHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCEEECCCCCHHHHHCCHHHHHHHHHH
VSKVTSYFEKDLNRESESEQYAYKRLHKDGSFFPTENKVKRIWYKGDYAWAGICRDITER
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
VKMAERIRESEKLAHIGQLTTSLAHEIRNPLSSIKMSIRMLLKSMDFDGDNKKTLEIADR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
EISHLERILTEMLDFARPVKLCFELLSINQLIYSCLEILKVTIREKEITVIEKLSKRMSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SMMDGNKIEHAIINILLNAIEVLPTGGIISIETHHRKLDHKPFISVEISDNGPGVTSEDL
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCEEEEEECCCCCCCCCCCC
PYIFNPFFSKKKKGTGLGLSNVKKVVDAHGGKIAASATSKGFSISFTIPVRKDHV
CHHHCHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MEIFEQFPLGVYVHFFQHKVRINRMNHKMELKDILLAHRKEINVKWSIVVDKHYCEQGYR
CCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEEHHHHHCCCC
QEPFEDTLAVNLEAVDANYAVLANRDFAPLDDFIEKIARKRFAAGFDLSDVQMAFEYYRT
CCCCCCHHEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ITIPIITKELPQNKMTPALERLNNCLSYTIIKFSDYFQNLHEKALKEHAEHLELEVMKRT
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELAELEAKYRVLVEEINDGYFVEKKGRIIFANHAFCDMHGYTPTEVINQPYINFVASEY
HHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCEEECCCCCHHHHHCCHHHHHHHHHH
VSKVTSYFEKDLNRESESEQYAYKRLHKDGSFFPTENKVKRIWYKGDYAWAGICRDITER
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
VKMAERIRESEKLAHIGQLTTSLAHEIRNPLSSIKMSIRMLLKSMDFDGDNKKTLEIADR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
EISHLERILTEMLDFARPVKLCFELLSINQLIYSCLEILKVTIREKEITVIEKLSKRMSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SMMDGNKIEHAIINILLNAIEVLPTGGIISIETHHRKLDHKPFISVEISDNGPGVTSEDL
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCEEEEEECCCCCCCCCCCC
PYIFNPFFSKKKKGTGLGLSNVKKVVDAHGGKIAASATSKGFSISFTIPVRKDHV
CHHHCHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]