Click here to switch to the map view.

The map label for this gene is ilvI [H]

Identifier: 85860818

GI number: 85860818

Start: 2982933

End: 2984639

Strand: Direct

Name: ilvI [H]

Synonym: SYN_01711

Alternate gene names: 85860818

Gene position: 2982933-2984639 (Clockwise)

Preceding gene: 85860817

Following gene: 85860819

Centisome position: 93.82

GC content: 56.18

Gene sequence:

>1707_bases
GTGAAACTCACCGGATCACAAGTCCTTTTTAAAGCCTTGCGGGAAGAAAAAGTAGATACTATTTTCGGTTATCCCGGGGG
CTCCGTCATCGATCTTTATGATGAATTGTTCCATCAGGAAGGAATCAAACACTATCTGGTGCGGCATGAACAGGGGGCCG
TCCACGCCGCGGACGGTTATGCGCGCGCTACCGGCAAAGTCGGGGTCTGCCTGGTGACTTCCGGTCCCGGCGCCACCAAC
ACCGTAACCGGCATTGCCAATGCCTATGCGGATTCCATCCCGATCGTCATCCTGACCGGGCAGGTTCCCACCCATATGAT
CGGCAAGGATGCCTTTCAGGAAGTGGACATTACAGGCATTACCCGTCCCTGCAGTAAGCATAACTTTCTGGTCAGAAAAA
TCGATGACCTGGGCCGTACTATAAGAGAAGCCTTCTACATCGCGCACAGCGGGCGTCCCGGTCCCGTTCTGGTCGATATC
CCTAAAGATATTCTACAGGGAACGCTGGAAGAAACAGAGCTTTTCATGGAAATTCATCTGCCTCCGGAGCCGCCCAAAGA
GCCGATCGACGAGGCCTGCGCCGCAGCCCTGACCATGATCCGGACCGCACGCAAACCGGTTATTTTCGCCGGCGGCGGGG
TTCTGCGGGGAAAAGCCGTTTCCGAGCTGATGACCTTTGCCAATTGCATCCATGCTCCGGTCACGGCATCGCTCATGGGA
CTGGGAGCTTTTCCCGGAACTCACCCGCGCTGGCTGGGCATGCTGGGCATGCACGGAACTTATTACGCCAACATGGCCGT
CAGTCACTGTGACCTGTTGATTGCCGTGGGTGTGCGCTTTGACGACCGTGTCACCTCCAAGGTGGCGGAATTCGCGCCTC
ACGCCAAAATTATTCATATCGACATCGATCCCGTTTCAATCAACAAGATCGTCAAGTCTCATGTTTCTATCATCAGCGAC
TGCCGGGAAGCTCTGCAGCGTCTTCTTTCCATGGCCGGTGGCGAAGTTCTGCTTTCCGCGGATCAAACCCGGGAATGGCT
GGAGCAGATTGAAAAATGGAAGAAGGCGGTTCCTCTGAGCTATTGCCGCAGCGCGAAGATCAAGCCTCAGTATGTCATCG
AAAAGCTCTACGAACTGACGCGGGGGGAGGCCATCATTACCACTGAAGTCGGTCAGAATCAGATGTGGGCAGCCCAGTTC
TACCGGTTCGATCACCCCGATACCTTCATCTCCTCGGGCGGACTGGGAACGATGGGATTCGGGCTTCCGGCGGCGATTGG
CGCCCAGGTAGCCTTTCCGGACCGGTTGGTCGTCGACATTGCCGGAGACGGCAGCATTCAGATGAACATCCAGGAACTGG
CTACCGCCGTGCATTACAATCTGCCGGTCAAGGTCGTCATCCTGAACAACGGCTTCCTGGGAATGGTCCGGCAGTGGCAG
GAAATGTTTTACGGCAGACGCTATTCCCAGACCGAGATGAAACAGGCGCTGGATTTCGTCAAACTGGCCGAGGCATTCGG
TGCCGTCGGGTTAAGGGCCACAAAACCGGAAGAAGTGGAAGAGGTTCTGCGCAAGGGGCTGGAAACTCCGAGGGCCGTCA
TGATGGATTTCCACGTGGAACCGGAAGAGCAGGTATACCCCATGGTCAGCCCGGGTGCGGGACTGACGGACATGAAGCTG
GGAAACCTGCGTCGGGATGCCGATTAA

Upstream 100 bases:

>100_bases
CGGCACCCGCAGAAGGATTCCGCATGAGGACGAAGGACTCTGCAGATTCCTGGAAAGAAGAATTTTTATTTCATAAATTA
ACAGTCAGGGGGTATTTCTC

Downstream 100 bases:

>100_bases
AGGAACTTCAACTCCGCCCATTCATTTTACTGACAGAAAGGTTTGCTGAAAAATGGAAATCAAGAAGAGTGTCATCTCAA
TTCTGGCCAGCAACAAGCCT

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKLTGSQVLFKALREEKVDTIFGYPGGSVIDLYDELFHQEGIKHYLVRHEQGAVHAADGYARATGKVGVCLVTSGPGATN
TVTGIANAYADSIPIVILTGQVPTHMIGKDAFQEVDITGITRPCSKHNFLVRKIDDLGRTIREAFYIAHSGRPGPVLVDI
PKDILQGTLEETELFMEIHLPPEPPKEPIDEACAAALTMIRTARKPVIFAGGGVLRGKAVSELMTFANCIHAPVTASLMG
LGAFPGTHPRWLGMLGMHGTYYANMAVSHCDLLIAVGVRFDDRVTSKVAEFAPHAKIIHIDIDPVSINKIVKSHVSIISD
CREALQRLLSMAGGEVLLSADQTREWLEQIEKWKKAVPLSYCRSAKIKPQYVIEKLYELTRGEAIITTEVGQNQMWAAQF
YRFDHPDTFISSGGLGTMGFGLPAAIGAQVAFPDRLVVDIAGDGSIQMNIQELATAVHYNLPVKVVILNNGFLGMVRQWQ
EMFYGRRYSQTEMKQALDFVKLAEAFGAVGLRATKPEEVEEVLRKGLETPRAVMMDFHVEPEEQVYPMVSPGAGLTDMKL
GNLRRDAD

Sequences:

>Translated_568_residues
MKLTGSQVLFKALREEKVDTIFGYPGGSVIDLYDELFHQEGIKHYLVRHEQGAVHAADGYARATGKVGVCLVTSGPGATN
TVTGIANAYADSIPIVILTGQVPTHMIGKDAFQEVDITGITRPCSKHNFLVRKIDDLGRTIREAFYIAHSGRPGPVLVDI
PKDILQGTLEETELFMEIHLPPEPPKEPIDEACAAALTMIRTARKPVIFAGGGVLRGKAVSELMTFANCIHAPVTASLMG
LGAFPGTHPRWLGMLGMHGTYYANMAVSHCDLLIAVGVRFDDRVTSKVAEFAPHAKIIHIDIDPVSINKIVKSHVSIISD
CREALQRLLSMAGGEVLLSADQTREWLEQIEKWKKAVPLSYCRSAKIKPQYVIEKLYELTRGEAIITTEVGQNQMWAAQF
YRFDHPDTFISSGGLGTMGFGLPAAIGAQVAFPDRLVVDIAGDGSIQMNIQELATAVHYNLPVKVVILNNGFLGMVRQWQ
EMFYGRRYSQTEMKQALDFVKLAEAFGAVGLRATKPEEVEEVLRKGLETPRAVMMDFHVEPEEQVYPMVSPGAGLTDMKL
GNLRRDAD
>Mature_568_residues
MKLTGSQVLFKALREEKVDTIFGYPGGSVIDLYDELFHQEGIKHYLVRHEQGAVHAADGYARATGKVGVCLVTSGPGATN
TVTGIANAYADSIPIVILTGQVPTHMIGKDAFQEVDITGITRPCSKHNFLVRKIDDLGRTIREAFYIAHSGRPGPVLVDI
PKDILQGTLEETELFMEIHLPPEPPKEPIDEACAAALTMIRTARKPVIFAGGGVLRGKAVSELMTFANCIHAPVTASLMG
LGAFPGTHPRWLGMLGMHGTYYANMAVSHCDLLIAVGVRFDDRVTSKVAEFAPHAKIIHIDIDPVSINKIVKSHVSIISD
CREALQRLLSMAGGEVLLSADQTREWLEQIEKWKKAVPLSYCRSAKIKPQYVIEKLYELTRGEAIITTEVGQNQMWAAQF
YRFDHPDTFISSGGLGTMGFGLPAAIGAQVAFPDRLVVDIAGDGSIQMNIQELATAVHYNLPVKVVILNNGFLGMVRQWQ
EMFYGRRYSQTEMKQALDFVKLAEAFGAVGLRATKPEEVEEVLRKGLETPRAVMMDFHVEPEEQVYPMVSPGAGLTDMKL
GNLRRDAD

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=25.5357142857143, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI21361361, Length=566, Percent_Identity=25.4416961130742, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=50.5263157894737, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=42.5531914893617, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786717, Length=558, Percent_Identity=34.0501792114695, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=28.6231884057971, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788716, Length=547, Percent_Identity=26.8738574040219, Blast_Score=182, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17531301, Length=558, Percent_Identity=27.0609318996416, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=558, Percent_Identity=27.0609318996416, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=531, Percent_Identity=25.8003766478343, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=45.9412780656304, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6321524, Length=535, Percent_Identity=24.8598130841122, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323073, Length=532, Percent_Identity=24.0601503759398, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=533, Percent_Identity=23.827392120075, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320123, Length=559, Percent_Identity=22.5402504472272, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=23.1083844580777, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922626, Length=561, Percent_Identity=24.2424242424242, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62352; Mature: 62352

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTGSQVLFKALREEKVDTIFGYPGGSVIDLYDELFHQEGIKHYLVRHEQGAVHAADGY
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCC
ARATGKVGVCLVTSGPGATNTVTGIANAYADSIPIVILTGQVPTHMIGKDAFQEVDITGI
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHCCCCCC
TRPCSKHNFLVRKIDDLGRTIREAFYIAHSGRPGPVLVDIPKDILQGTLEETELFMEIHL
CCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCHHHHHCHHHHCEEEEEEEC
PPEPPKEPIDEACAAALTMIRTARKPVIFAGGGVLRGKAVSELMTFANCIHAPVTASLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHHHHHHHHHHCCCHHHHHHH
LGAFPGTHPRWLGMLGMHGTYYANMAVSHCDLLIAVGVRFDDRVTSKVAEFAPHAKIIHI
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCEEEEE
DIDPVSINKIVKSHVSIISDCREALQRLLSMAGGEVLLSADQTREWLEQIEKWKKAVPLS
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCHH
YCRSAKIKPQYVIEKLYELTRGEAIITTEVGQNQMWAAQFYRFDHPDTFISSGGLGTMGF
HHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCCHHHCCCCCCCCCC
GLPAAIGAQVAFPDRLVVDIAGDGSIQMNIQELATAVHYNLPVKVVILNNGFLGMVRQWQ
CCHHHHCCCEECCCEEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
EMFYGRRYSQTEMKQALDFVKLAEAFGAVGLRATKPEEVEEVLRKGLETPRAVMMDFHVE
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCC
PEEQVYPMVSPGAGLTDMKLGNLRRDAD
CHHHCCCEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLTGSQVLFKALREEKVDTIFGYPGGSVIDLYDELFHQEGIKHYLVRHEQGAVHAADGY
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCC
ARATGKVGVCLVTSGPGATNTVTGIANAYADSIPIVILTGQVPTHMIGKDAFQEVDITGI
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHCCCCCC
TRPCSKHNFLVRKIDDLGRTIREAFYIAHSGRPGPVLVDIPKDILQGTLEETELFMEIHL
CCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCHHHHHCHHHHCEEEEEEEC
PPEPPKEPIDEACAAALTMIRTARKPVIFAGGGVLRGKAVSELMTFANCIHAPVTASLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEECHHHHHHHHHHHHHHCCCHHHHHHH
LGAFPGTHPRWLGMLGMHGTYYANMAVSHCDLLIAVGVRFDDRVTSKVAEFAPHAKIIHI
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCEEEEE
DIDPVSINKIVKSHVSIISDCREALQRLLSMAGGEVLLSADQTREWLEQIEKWKKAVPLS
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCHH
YCRSAKIKPQYVIEKLYELTRGEAIITTEVGQNQMWAAQFYRFDHPDTFISSGGLGTMGF
HHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCCHHHCCCCCCCCCC
GLPAAIGAQVAFPDRLVVDIAGDGSIQMNIQELATAVHYNLPVKVVILNNGFLGMVRQWQ
CCHHHHCCCEECCCEEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
EMFYGRRYSQTEMKQALDFVKLAEAFGAVGLRATKPEEVEEVLRKGLETPRAVMMDFHVE
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCC
PEEQVYPMVSPGAGLTDMKLGNLRRDAD
CHHHCCCEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]