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The map label for this gene is kamA [H]

Identifier: 85860656

GI number: 85860656

Start: 2827849

End: 2829309

Strand: Direct

Name: kamA [H]

Synonym: SYN_02634

Alternate gene names: 85860656

Gene position: 2827849-2829309 (Clockwise)

Preceding gene: 85860654

Following gene: 85860657

Centisome position: 88.95

GC content: 49.83

Gene sequence:

>1461_bases
TTGGGAGGGAAAACGCCTGCAAATATCGAATATCAAGTTATGCGCGCTTTGCGTTCTGTCGTCCGGACAGTGGACTTTAA
GCAATTCCACAAAATACATGTTCCCCCGCCAACAAAGTCATCGTTCGTAAAGCGCGAACATCCCGAAAAGAAAGGATTCC
GTATGCTGACTCAATCCCAGCACAAAATACTCAATGAAATCGATGGAAGTTCGGTCCGCAACTGGAAGGATTGGAAGTGG
CAGTTGAGACACTGCATAAAAGATCTGGACACGTTCGAGACACTTCTTGACATCCGCTTGCCGGAAACCCTGCGCCGGCA
GTTCAAATTGATCGTCGAAAAATTTCCCATGTCGATAACGCCTTATTATCTCTCCCTGATTGACACGGAAGATCTGGAAA
ATGATCCGGTTTTCAAACAAAGCTTCCCTGCAATCAACGAATTGGATGTTCAGTCAACGGACATGTCGGATCCTCTGCAT
GAGGATCGGGACAGCCCGGTACCGGGTTTGACACACAGGTATCCGGACCGTGTTTTGCTGCTGATCAGTAACACTTGTGC
CATGTATTGCCGTCACTGCACAAGAAAGCGCCGCGTGGGCGATCGGGATACAATTCCCAGCCGTGAGCAGATCATGAAGG
GAATCGAATACATTAGAGACACACCGCAGGTCAGGGATGTCCTTTTAAGCGGTGGTGATCCCTTTCTCCTGTCGACGGAT
TACCTTGACTGGATTCTCATCGAATTGAAGAAGATCGAGCACGTCGAAGTCATAAGGATCGGAACACGTACGCCTGCCGT
ACTTCCCTATAGAATTACCGATGAACTGGTTGAAATGCTGAAAAAACATCATCCTCTCTGGATAAACACCCATTTCAACC
ACCCCAGGGAATTAACGGCCTCTTCAAGAGCCGCCCTCAGAAAACTTGCAGATGCCGGGATACCCCTCGGCAATCAATCG
GTTCTGTTGTCTGGGGTCAATGACTGTCCCCGGATCATGCGTTCCCTGGTCCATAAACTCGTCGCTAATCGTGTCCGGCC
TTACTATCTCTATCAATGCGATCTGTCCGAAGGGCTCAGTCATTTTCGAACTCCCGTAGGAAAGGGAATTGAAATTCTCG
AAAGCCTGATAGGCCACACAAGCGGCTTCTGCGTTCCGACCTATGTGATCGATGCCCCGGCCGGTGGAGGAAAAATTCCG
GTCATGCCGAACTATCTCATCTCCTGGTCCACAAACAAGGTGGTTTTACGGAATTATGAAGGAGTCATAACCACTTACAA
GGAACCCGATTCTTACGAACCCACTTTCTGCGACCGGAACTGCAAGGACTGCAACCTCCAGCTCTCACTCGAAGACGCGG
GTGAATTCCGTGCCGTAGGCATAGAAAAACTGCTGGCCGACTACGACGACACCATTGCCCTGGTTCCCAGGAATAACGAT
CGCCACAGGAGAAGAGGATGA

Upstream 100 bases:

>100_bases
CTTTTTCTGTTTTTTCTTGACATGGCCCTTGAAATCTTTATTTTCCTACAAGAATTATTCTAACATGAAATGTTTCTACT
GAAATGATCCATCATTAAAC

Downstream 100 bases:

>100_bases
ACTCCTTCGTAAAAAATTCTCTGCCGGACCGGATAGAAAAGCTTCCCTGCGGCTCAGTCATCCAGCATGGTCCATACAAT
GAGCGGATATACCTGCTGAA

Product: L-lysine 2,3-aminomutase

Products: NA

Alternate protein names: LAM; KAM [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGGKTPANIEYQVMRALRSVVRTVDFKQFHKIHVPPPTKSSFVKREHPEKKGFRMLTQSQHKILNEIDGSSVRNWKDWKW
QLRHCIKDLDTFETLLDIRLPETLRRQFKLIVEKFPMSITPYYLSLIDTEDLENDPVFKQSFPAINELDVQSTDMSDPLH
EDRDSPVPGLTHRYPDRVLLLISNTCAMYCRHCTRKRRVGDRDTIPSREQIMKGIEYIRDTPQVRDVLLSGGDPFLLSTD
YLDWILIELKKIEHVEVIRIGTRTPAVLPYRITDELVEMLKKHHPLWINTHFNHPRELTASSRAALRKLADAGIPLGNQS
VLLSGVNDCPRIMRSLVHKLVANRVRPYYLYQCDLSEGLSHFRTPVGKGIEILESLIGHTSGFCVPTYVIDAPAGGGKIP
VMPNYLISWSTNKVVLRNYEGVITTYKEPDSYEPTFCDRNCKDCNLQLSLEDAGEFRAVGIEKLLADYDDTIALVPRNND
RHRRRG

Sequences:

>Translated_486_residues
MGGKTPANIEYQVMRALRSVVRTVDFKQFHKIHVPPPTKSSFVKREHPEKKGFRMLTQSQHKILNEIDGSSVRNWKDWKW
QLRHCIKDLDTFETLLDIRLPETLRRQFKLIVEKFPMSITPYYLSLIDTEDLENDPVFKQSFPAINELDVQSTDMSDPLH
EDRDSPVPGLTHRYPDRVLLLISNTCAMYCRHCTRKRRVGDRDTIPSREQIMKGIEYIRDTPQVRDVLLSGGDPFLLSTD
YLDWILIELKKIEHVEVIRIGTRTPAVLPYRITDELVEMLKKHHPLWINTHFNHPRELTASSRAALRKLADAGIPLGNQS
VLLSGVNDCPRIMRSLVHKLVANRVRPYYLYQCDLSEGLSHFRTPVGKGIEILESLIGHTSGFCVPTYVIDAPAGGGKIP
VMPNYLISWSTNKVVLRNYEGVITTYKEPDSYEPTFCDRNCKDCNLQLSLEDAGEFRAVGIEKLLADYDDTIALVPRNND
RHRRRG
>Mature_485_residues
GGKTPANIEYQVMRALRSVVRTVDFKQFHKIHVPPPTKSSFVKREHPEKKGFRMLTQSQHKILNEIDGSSVRNWKDWKWQ
LRHCIKDLDTFETLLDIRLPETLRRQFKLIVEKFPMSITPYYLSLIDTEDLENDPVFKQSFPAINELDVQSTDMSDPLHE
DRDSPVPGLTHRYPDRVLLLISNTCAMYCRHCTRKRRVGDRDTIPSREQIMKGIEYIRDTPQVRDVLLSGGDPFLLSTDY
LDWILIELKKIEHVEVIRIGTRTPAVLPYRITDELVEMLKKHHPLWINTHFNHPRELTASSRAALRKLADAGIPLGNQSV
LLSGVNDCPRIMRSLVHKLVANRVRPYYLYQCDLSEGLSHFRTPVGKGIEILESLIGHTSGFCVPTYVIDAPAGGGKIPV
MPNYLISWSTNKVVLRNYEGVITTYKEPDSYEPTFCDRNCKDCNLQLSLEDAGEFRAVGIEKLLADYDDTIALVPRNNDR
HRRRG

Specific function: Catalyzes the interconversion of L-alpha-lysine and L- beta-lysine [H]

COG id: COG1509

COG function: function code E; Lysine 2,3-aminomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KamA family [H]

Homologues:

Organism=Escherichia coli, GI1790589, Length=333, Percent_Identity=33.6336336336336, Blast_Score=173, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003739
- InterPro:   IPR022459
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =5.4.3.2 [H]

Molecular weight: Translated: 55968; Mature: 55837

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGKTPANIEYQVMRALRSVVRTVDFKQFHKIHVPPPTKSSFVKREHPEKKGFRMLTQSQ
CCCCCCCCCHHHHHHHHHHHHHHHCHHHHCEEECCCCCCHHHHHHCCCHHHHHHHHHHHH
HKILNEIDGSSVRNWKDWKWQLRHCIKDLDTFETLLDIRLPETLRRQFKLIVEKFPMSIT
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
PYYLSLIDTEDLENDPVFKQSFPAINELDVQSTDMSDPLHEDRDSPVPGLTHRYPDRVLL
CHHHEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
LISNTCAMYCRHCTRKRRVGDRDTIPSREQIMKGIEYIRDTPQVRDVLLSGGDPFLLSTD
EEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEHH
YLDWILIELKKIEHVEVIRIGTRTPAVLPYRITDELVEMLKKHHPLWINTHFNHPRELTA
HHHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHCCCEEEECCCCCCHHHHH
SSRAALRKLADAGIPLGNQSVLLSGVNDCPRIMRSLVHKLVANRVRPYYLYQCDLSEGLS
HHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHH
HFRTPVGKGIEILESLIGHTSGFCVPTYVIDAPAGGGKIPVMPNYLISWSTNKVVLRNYE
HHHCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCEEEEECCC
GVITTYKEPDSYEPTFCDRNCKDCNLQLSLEDAGEFRAVGIEKLLADYDDTIALVPRNND
CEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCC
RHRRRG
CCCCCC
>Mature Secondary Structure 
GGKTPANIEYQVMRALRSVVRTVDFKQFHKIHVPPPTKSSFVKREHPEKKGFRMLTQSQ
CCCCCCCCHHHHHHHHHHHHHHHCHHHHCEEECCCCCCHHHHHHCCCHHHHHHHHHHHH
HKILNEIDGSSVRNWKDWKWQLRHCIKDLDTFETLLDIRLPETLRRQFKLIVEKFPMSIT
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
PYYLSLIDTEDLENDPVFKQSFPAINELDVQSTDMSDPLHEDRDSPVPGLTHRYPDRVLL
CHHHEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
LISNTCAMYCRHCTRKRRVGDRDTIPSREQIMKGIEYIRDTPQVRDVLLSGGDPFLLSTD
EEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEHH
YLDWILIELKKIEHVEVIRIGTRTPAVLPYRITDELVEMLKKHHPLWINTHFNHPRELTA
HHHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHCCCEEEECCCCCCHHHHH
SSRAALRKLADAGIPLGNQSVLLSGVNDCPRIMRSLVHKLVANRVRPYYLYQCDLSEGLS
HHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHH
HFRTPVGKGIEILESLIGHTSGFCVPTYVIDAPAGGGKIPVMPNYLISWSTNKVVLRNYE
HHHCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCEEEEECCC
GVITTYKEPDSYEPTFCDRNCKDCNLQLSLEDAGEFRAVGIEKLLADYDDTIALVPRNND
CEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCC
RHRRRG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10629195 [H]