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The map label for this gene is pleD [H]

Identifier: 85860641

GI number: 85860641

Start: 2811427

End: 2812923

Strand: Direct

Name: pleD [H]

Synonym: SYN_01079

Alternate gene names: 85860641

Gene position: 2811427-2812923 (Clockwise)

Preceding gene: 85860640

Following gene: 85860644

Centisome position: 88.43

GC content: 56.11

Gene sequence:

>1497_bases
ATGATTAATTTGTTGACCACTCAAAACCTTGTTATTGGAAAATCACCTCAGGCAATCCTGGCGCACATCCGTCACGAGTT
ACGTGGCCCGATCAACGCCATTCTCGGTTACAGCGAGATGTGGCTGAATGATGATTCCCTCCGGGCCATTCCAAATCTGC
ACGCCAATATGGCAAAACTGCATGCAGCCGGACTGCAGTTGCGATCTCTGATCCGCGACATACTGGATCCGGCGAAGCAC
GACGGAAGGAAGGAACAGGACGTTCGGGATCTGGGCGTGATTCTGCGCCACGCGTTCCGCATGCCGGTGTGGGCGATTCT
GGGCTATAACGCCATGTTGACGGAAAACAAAGCTGAGACGGTTGATCGGGCAACGGAATTTCTCGCTGATCTGCACCGTA
TCGAGGATGCAACCCAGCGCCTGTTTACTCAGGCGGATATGATCGCCACGACCGGCTGTCTGCCTGTCGATCAAACCATC
TATAAGCCGTCTAAAGAACTGAGGGTCCAGCTGCAGACCATCGTGGCGAGATTCCGTTCCGCCAGACATCCCCGGTCGCA
ATACGCCGCCGCTGAGAAAGGAAAGATCCTTGTTGTTGATGATGACACAGCCAACTGTGATCTGCTCTCCCGCCAGCTTG
ACCGTCTGGGTTATTCGGTGACTGTGGCACAGAACGGCATCATCGCCCTCGACCTGCTTTCAACGCAAACCTTCGACCTG
GTGCTTCTCGATATCCTCATGCCGGTCGTGAGCGGGATCGAAGTCCTGAAATGCCTGCGCTGCGCCTGGTCCATCGCGGA
ATTGCCGGTTATCATGGTCACGGCCCGGGATCGCAACGACGACATCGTGTCCGCGCTGGATTGCGGAGCAAATGACTATG
TGGCAAAACCCTTCTCACTGCCCGTGGTTCTTGCGCGGGTGCGCACTCAAATGATGCTGAAGCAGACGGTTGCCGACCTG
GAGAATTTAAACCGGAAGCTGGAAAAGCTGTCCCTTCATGACGGATTGACCAATATCCCCAATCGGCGATGCTTTAATGA
AACCTTTGAACGCGAACAGCTCCGGACGAGACGGAACGGGATGCCCCTTTCCCTGATTCTGCTGGATGTCGATTTCTTCA
AACGGTACAACGACACTTATGGACATGAGGCCGGCGATCAGGTTCTCTGCCGGGTGGCGGATCTGCTGGTCTCAGCGACC
GACCGCGGAGGAGACATGGTCTTCCGCTACGGCGGGGAGGAATTCGTGATCCTTTTGCCGGAAACCCCGGCTGCCGGGGC
AAGGTTCGTGGCGGAACGGATTCATTCGGCGATTCAGCAGCTGGCCATTCCTCACCTGACCTCCACCGTCGCCCATCACC
TGACCGCCAGCATGGGTATTGCCACCGCCACGCCTTCAGACAAGGAAAGCTATTACACGCTTTTCGAAAGGGCCGATTCT
CAGCTTTACAAGGTGAAAGCCAGCGGACGCAACAGCCTGCGCCAGTCGCCTGACTGA

Upstream 100 bases:

>100_bases
CTCCGGGCCGGCTGCGATGAATACGACAGCAAGCCCATCGAATTTCCCCGTCTGCTGGAAAAAATTGCCGCCCTGCTTGA
TTCGTCGGGAGGTAAAGGTT

Downstream 100 bases:

>100_bases
CAAACGTTCACAAAGAATCCGGTTCAGCTGAACGACGGCAGCCTTCCCGAAGCTTGCTCCCGCTGGGACCATCAGGCTTC
TCCCTCGTCGTTCGGCATCT

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MINLLTTQNLVIGKSPQAILAHIRHELRGPINAILGYSEMWLNDDSLRAIPNLHANMAKLHAAGLQLRSLIRDILDPAKH
DGRKEQDVRDLGVILRHAFRMPVWAILGYNAMLTENKAETVDRATEFLADLHRIEDATQRLFTQADMIATTGCLPVDQTI
YKPSKELRVQLQTIVARFRSARHPRSQYAAAEKGKILVVDDDTANCDLLSRQLDRLGYSVTVAQNGIIALDLLSTQTFDL
VLLDILMPVVSGIEVLKCLRCAWSIAELPVIMVTARDRNDDIVSALDCGANDYVAKPFSLPVVLARVRTQMMLKQTVADL
ENLNRKLEKLSLHDGLTNIPNRRCFNETFEREQLRTRRNGMPLSLILLDVDFFKRYNDTYGHEAGDQVLCRVADLLVSAT
DRGGDMVFRYGGEEFVILLPETPAAGARFVAERIHSAIQQLAIPHLTSTVAHHLTASMGIATATPSDKESYYTLFERADS
QLYKVKASGRNSLRQSPD

Sequences:

>Translated_498_residues
MINLLTTQNLVIGKSPQAILAHIRHELRGPINAILGYSEMWLNDDSLRAIPNLHANMAKLHAAGLQLRSLIRDILDPAKH
DGRKEQDVRDLGVILRHAFRMPVWAILGYNAMLTENKAETVDRATEFLADLHRIEDATQRLFTQADMIATTGCLPVDQTI
YKPSKELRVQLQTIVARFRSARHPRSQYAAAEKGKILVVDDDTANCDLLSRQLDRLGYSVTVAQNGIIALDLLSTQTFDL
VLLDILMPVVSGIEVLKCLRCAWSIAELPVIMVTARDRNDDIVSALDCGANDYVAKPFSLPVVLARVRTQMMLKQTVADL
ENLNRKLEKLSLHDGLTNIPNRRCFNETFEREQLRTRRNGMPLSLILLDVDFFKRYNDTYGHEAGDQVLCRVADLLVSAT
DRGGDMVFRYGGEEFVILLPETPAAGARFVAERIHSAIQQLAIPHLTSTVAHHLTASMGIATATPSDKESYYTLFERADS
QLYKVKASGRNSLRQSPD
>Mature_498_residues
MINLLTTQNLVIGKSPQAILAHIRHELRGPINAILGYSEMWLNDDSLRAIPNLHANMAKLHAAGLQLRSLIRDILDPAKH
DGRKEQDVRDLGVILRHAFRMPVWAILGYNAMLTENKAETVDRATEFLADLHRIEDATQRLFTQADMIATTGCLPVDQTI
YKPSKELRVQLQTIVARFRSARHPRSQYAAAEKGKILVVDDDTANCDLLSRQLDRLGYSVTVAQNGIIALDLLSTQTFDL
VLLDILMPVVSGIEVLKCLRCAWSIAELPVIMVTARDRNDDIVSALDCGANDYVAKPFSLPVVLARVRTQMMLKQTVADL
ENLNRKLEKLSLHDGLTNIPNRRCFNETFEREQLRTRRNGMPLSLILLDVDFFKRYNDTYGHEAGDQVLCRVADLLVSAT
DRGGDMVFRYGGEEFVILLPETPAAGARFVAERIHSAIQQLAIPHLTSTVAHHLTASMGIATATPSDKESYYTLFERADS
QLYKVKASGRNSLRQSPD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=211, Percent_Identity=36.0189573459716, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI145693134, Length=191, Percent_Identity=36.6492146596859, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=33.8983050847458, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=38.75, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87082007, Length=183, Percent_Identity=32.2404371584699, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082012, Length=162, Percent_Identity=36.4197530864198, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787802, Length=213, Percent_Identity=30.9859154929577, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=31.3609467455621, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=31.4917127071823, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786784, Length=115, Percent_Identity=36.5217391304348, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=32.9268292682927, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786599, Length=135, Percent_Identity=32.5925925925926, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=38.135593220339, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=37.2881355932203, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788191, Length=145, Percent_Identity=34.4827586206897, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788550, Length=148, Percent_Identity=36.4864864864865, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790299, Length=116, Percent_Identity=37.9310344827586, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790437, Length=138, Percent_Identity=36.9565217391304, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787541, Length=189, Percent_Identity=26.4550264550265, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786911, Length=116, Percent_Identity=36.2068965517241, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI2367329, Length=114, Percent_Identity=43.859649122807, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=173, Percent_Identity=31.2138728323699, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=253, Percent_Identity=25.296442687747, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789402, Length=117, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788394, Length=115, Percent_Identity=33.0434782608696, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790552, Length=114, Percent_Identity=35.9649122807018, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787229, Length=113, Percent_Identity=31.858407079646, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=131, Percent_Identity=31.2977099236641, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081974, Length=195, Percent_Identity=27.1794871794872, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI145693157, Length=108, Percent_Identity=37.962962962963, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=104, Percent_Identity=35.5769230769231, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55715; Mature: 55715

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLLTTQNLVIGKSPQAILAHIRHELRGPINAILGYSEMWLNDDSLRAIPNLHANMAKL
CCEEEECCCEEECCCHHHHHHHHHHHHCCHHHHHHCCHHHHCCCCHHHHCCCHHHHHHHH
HAAGLQLRSLIRDILDPAKHDGRKEQDVRDLGVILRHAFRMPVWAILGYNAMLTENKAET
HHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCEECCCHHHH
VDRATEFLADLHRIEDATQRLFTQADMIATTGCLPVDQTIYKPSKELRVQLQTIVARFRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
ARHPRSQYAAAEKGKILVVDDDTANCDLLSRQLDRLGYSVTVAQNGIIALDLLSTQTFDL
CCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCEEEEEECCCCHHHH
VLLDILMPVVSGIEVLKCLRCAWSIAELPVIMVTARDRNDDIVSALDCGANDYVAKPFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCHH
PVVLARVRTQMMLKQTVADLENLNRKLEKLSLHDGLTNIPNRRCFNETFEREQLRTRRNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
MPLSLILLDVDFFKRYNDTYGHEAGDQVLCRVADLLVSATDRGGDMVFRYGGEEFVILLP
CCEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEC
ETPAAGARFVAERIHSAIQQLAIPHLTSTVAHHLTASMGIATATPSDKESYYTLFERADS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCC
QLYKVKASGRNSLRQSPD
CEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MINLLTTQNLVIGKSPQAILAHIRHELRGPINAILGYSEMWLNDDSLRAIPNLHANMAKL
CCEEEECCCEEECCCHHHHHHHHHHHHCCHHHHHHCCHHHHCCCCHHHHCCCHHHHHHHH
HAAGLQLRSLIRDILDPAKHDGRKEQDVRDLGVILRHAFRMPVWAILGYNAMLTENKAET
HHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCEECCCHHHH
VDRATEFLADLHRIEDATQRLFTQADMIATTGCLPVDQTIYKPSKELRVQLQTIVARFRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
ARHPRSQYAAAEKGKILVVDDDTANCDLLSRQLDRLGYSVTVAQNGIIALDLLSTQTFDL
CCCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCEEEEEECCCCHHHH
VLLDILMPVVSGIEVLKCLRCAWSIAELPVIMVTARDRNDDIVSALDCGANDYVAKPFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCHH
PVVLARVRTQMMLKQTVADLENLNRKLEKLSLHDGLTNIPNRRCFNETFEREQLRTRRNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
MPLSLILLDVDFFKRYNDTYGHEAGDQVLCRVADLLVSATDRGGDMVFRYGGEEFVILLP
CCEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEC
ETPAAGARFVAERIHSAIQQLAIPHLTSTVAHHLTASMGIATATPSDKESYYTLFERADS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCC
QLYKVKASGRNSLRQSPD
CEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA