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The map label for this gene is rnhA

Identifier: 85860563

GI number: 85860563

Start: 2740502

End: 2741023

Strand: Direct

Name: rnhA

Synonym: SYN_00291

Alternate gene names: 85860563

Gene position: 2740502-2741023 (Clockwise)

Preceding gene: 85860561

Following gene: 85860566

Centisome position: 86.2

GC content: 58.81

Gene sequence:

>522_bases
ATGAAAGCAACCAGCAAAGCGAAAACACATCCCCCCGGGGCAACCGCTGCGAAGGATCCTCAAAAACAGGTCATCATATA
CACCGACGGGGCCTGCCTGGGAAATCCAGGACCGGGAGGGTACGGGGTTGTTCTTCTTTATGGGGAGCACCGGAAAGAAC
TCTCCGGCGGCTATCGGCTCACTACGAACAACCGGATGGAGATCCTCGCGGCCATCAAGGGGCTGGAGGCCCTGAAGAGC
GCCTGCTCGGTCACCCTTTATTCGGATTCACAATATCTGGTCAATGCCATCAACAAGGGATGGGCGCAACGCTGGAAGGC
GAACGGCTGGAAACGGAACGCCCGGGAAAAGGCGCTCAATCCCGATCTCTGGGAACGGCTGCTGGAACTCTGCTCCCGCC
ATGATATCACTTTTGTCTGGGTGCGTGGGCACGCGAACAACAAGGAAAACGAACGCTGCGATGTCCTGTCCAAGGAGGCC
GCGGGACGGGCCGACCTGAAAGCCGATCCGGGCTACCCCTGA

Upstream 100 bases:

>100_bases
GAACATCCCGAGACGGAATAATTTATACGGAATAATTTATTGGGAAGGGGGCCCTGCTCATGATATATGCCCTTTTCAAA
AGAAAGGCGTCCCGAAATAC

Downstream 100 bases:

>100_bases
GTTTGCCTGAAACCGGAATGACTATACAACCGTTTATTCCTTTTCCCCTTCGGCCTTCCCGTTTTTTTCCAGCTTTTTTG
CCGCCTTCTTGTCCTGTTTC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MKATSKAKTHPPGATAAKDPQKQVIIYTDGACLGNPGPGGYGVVLLYGEHRKELSGGYRLTTNNRMEILAAIKGLEALKS
ACSVTLYSDSQYLVNAINKGWAQRWKANGWKRNAREKALNPDLWERLLELCSRHDITFVWVRGHANNKENERCDVLSKEA
AGRADLKADPGYP

Sequences:

>Translated_173_residues
MKATSKAKTHPPGATAAKDPQKQVIIYTDGACLGNPGPGGYGVVLLYGEHRKELSGGYRLTTNNRMEILAAIKGLEALKS
ACSVTLYSDSQYLVNAINKGWAQRWKANGWKRNAREKALNPDLWERLLELCSRHDITFVWVRGHANNKENERCDVLSKEA
AGRADLKADPGYP
>Mature_173_residues
MKATSKAKTHPPGATAAKDPQKQVIIYTDGACLGNPGPGGYGVVLLYGEHRKELSGGYRLTTNNRMEILAAIKGLEALKS
ACSVTLYSDSQYLVNAINKGWAQRWKANGWKRNAREKALNPDLWERLLELCSRHDITFVWVRGHANNKENERCDVLSKEA
AGRADLKADPGYP

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=147, Percent_Identity=34.6938775510204, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1786408, Length=151, Percent_Identity=52.317880794702, Blast_Score=155, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI115532542, Length=142, Percent_Identity=33.8028169014084, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137476, Length=153, Percent_Identity=32.0261437908497, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI45552509, Length=153, Percent_Identity=32.0261437908497, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_SYNAS (Q2LWY9)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_462765.1
- ProteinModelPortal:   Q2LWY9
- STRING:   Q2LWY9
- GeneID:   3882710
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_00291
- NMPDR:   fig|56780.10.peg.2707
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q2LWY9
- BioCyc:   SACI56780:SYN_00291-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 19095; Mature: 19095

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATSKAKTHPPGATAAKDPQKQVIIYTDGACLGNPGPGGYGVVLLYGEHRKELSGGYRL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCHHHCCCCEEE
TTNNRMEILAAIKGLEALKSACSVTLYSDSQYLVNAINKGWAQRWKANGWKRNAREKALN
ECCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
PDLWERLLELCSRHDITFVWVRGHANNKENERCDVLSKEAAGRADLKADPGYP
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKATSKAKTHPPGATAAKDPQKQVIIYTDGACLGNPGPGGYGVVLLYGEHRKELSGGYRL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCHHHCCCCEEE
TTNNRMEILAAIKGLEALKSACSVTLYSDSQYLVNAINKGWAQRWKANGWKRNAREKALN
ECCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
PDLWERLLELCSRHDITFVWVRGHANNKENERCDVLSKEAAGRADLKADPGYP
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA