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The map label for this gene is zraS [H]

Identifier: 85860539

GI number: 85860539

Start: 2718597

End: 2720108

Strand: Direct

Name: zraS [H]

Synonym: SYN_01808

Alternate gene names: 85860539

Gene position: 2718597-2720108 (Clockwise)

Preceding gene: 85860538

Following gene: 85860543

Centisome position: 85.51

GC content: 54.17

Gene sequence:

>1512_bases
ATGACACTGAAAACACGACTGATGATCATCACTCTGTTCGGCCTGGCCATCACCATGGCGCTGTGGGGCTGGATTCAGCT
CAGTGTTCTGGACAGAATTCTGCGGGAACAGCAATATAAAAGACTCGATAACATCGCGGAGACCTTGAGCGCCTTCTATC
AGCGTTTCCCCTCCGCCCGCGGCCTGTCCGCGCTGGACACCGCGCTGAAAGATCATATCCAGACGGATGTCCGCCTGGCC
CGCATCGATATCATTTCCCTGTCCGGAGACGACGTTGATTATATTGCCGGAGCCGGACGCGTACCCTACGAATGGCCGGA
GAATCTGATTATCCGGACAGACGGAAAAATGGAGAAGCACCATTATGCCCTGCAGACGGAAGCCGGTTCGGCGATTGGTC
TTCTTTATCCTGTTCATCCCGAAAAAGGCTCATCGGCAAAAACCATGATCGGCGTCATTCTTTTTTCCCAATCCGATATG
GAGATTTTGAGCCGGGCCCGGTGGATGCTTTTCTTCAGCACGCTGGGGCTTCTCGTCATTATCCTGCTCGTGATGGCCGT
CGGCTACGATTTTCTCATCGGCAGGCCTCTCCGACGGATTATCGAAACGATCGATCACTTTCGTACGGGAAAGCAGGCCA
GCCGTATTCCCATTGACCGTTCCGATGAATGGGGACAGCTGGCTGATCATTTCAACGTAATGGCCGGAGAGATAGAAAAT
TTCATAGCCCAGAACCAGGAATTGAACAGGGGATTGGAACTCCGGGTCAAGGAAGCCACTCTCAAGGTGGTTCAGCTGCA
GAGACAGGTCAATCAACTTCAGCAGTTGAAAGCCCTGGGATATCTGACGGCCACACTGGCCCATGATTTAGGCACGCCTT
TGCATTCCATCGCCGGCATGACCCAACTCCTCCTGGAACGGGACGAATGGCCGCCTGATACCGCCCGCAAACTGGAATTG
ATCCTGCAGCAAACTCAAAGGCTCAACACGGTGATTCAGAATGTCCGCCGCGCAACCCGTCTGCCGGAGCCTCATTTCGA
ATCGATCCCGGTACAGAGACTGCTCGACGATACCCTGCCGCTTGTTGAACCGGTCATTCAGAAATCGAAAGTGGAACTCC
TTGTCCAGATCGCCCCGGACACGCCCCCGTTATATGTAGATAACTACCGTGTTCAGACAGCCCTGTTCAATCTCATCCAG
AATGCCCTGGAAGCCATGCCGCATGGGGGGAAAATTTTTGTTTCCGCCCGGGTTTCCAATGACCGCCAGGCGATCGCCGT
AACCGTTCAGGACACCGGTCCAGGCATCCCTCCATCCATGATGGGAAAGATATGTGAACCCTTCTTCAGCACCCATCCGG
GCGAAGGCATCCGCGGCCTGGGACTGGCTATCGTCCAGGACATTGTCAAGGCCCATGCCGGTTCAATTGATATTCAAAGT
CAGCCTCACAAGGGAACATGCATTACGCTCTTTTTCCCGATCGCTTGCAGGCTGCAGGAAATCCCCGGGTAA

Upstream 100 bases:

>100_bases
TCTTTTTTTTCATGAAAATCATTTTTGTTTCCTGTAGAGAAAGGATCGTCGGGCAGAGAAAAAAGATTTCTCCGCCAATA
ATTCGGCTGAGGACAAAACC

Downstream 100 bases:

>100_bases
ATAAGGAAGGGATCAACATGAAGGGATAAAAAGACTGCAGGAAGGAGACGCACGGAAGGCTCCCGGCTGTTTTTCAGGAA
CAGGATCGTGATGGCGGGAA

Product: signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTLKTRLMIITLFGLAITMALWGWIQLSVLDRILREQQYKRLDNIAETLSAFYQRFPSARGLSALDTALKDHIQTDVRLA
RIDIISLSGDDVDYIAGAGRVPYEWPENLIIRTDGKMEKHHYALQTEAGSAIGLLYPVHPEKGSSAKTMIGVILFSQSDM
EILSRARWMLFFSTLGLLVIILLVMAVGYDFLIGRPLRRIIETIDHFRTGKQASRIPIDRSDEWGQLADHFNVMAGEIEN
FIAQNQELNRGLELRVKEATLKVVQLQRQVNQLQQLKALGYLTATLAHDLGTPLHSIAGMTQLLLERDEWPPDTARKLEL
ILQQTQRLNTVIQNVRRATRLPEPHFESIPVQRLLDDTLPLVEPVIQKSKVELLVQIAPDTPPLYVDNYRVQTALFNLIQ
NALEAMPHGGKIFVSARVSNDRQAIAVTVQDTGPGIPPSMMGKICEPFFSTHPGEGIRGLGLAIVQDIVKAHAGSIDIQS
QPHKGTCITLFFPIACRLQEIPG

Sequences:

>Translated_503_residues
MTLKTRLMIITLFGLAITMALWGWIQLSVLDRILREQQYKRLDNIAETLSAFYQRFPSARGLSALDTALKDHIQTDVRLA
RIDIISLSGDDVDYIAGAGRVPYEWPENLIIRTDGKMEKHHYALQTEAGSAIGLLYPVHPEKGSSAKTMIGVILFSQSDM
EILSRARWMLFFSTLGLLVIILLVMAVGYDFLIGRPLRRIIETIDHFRTGKQASRIPIDRSDEWGQLADHFNVMAGEIEN
FIAQNQELNRGLELRVKEATLKVVQLQRQVNQLQQLKALGYLTATLAHDLGTPLHSIAGMTQLLLERDEWPPDTARKLEL
ILQQTQRLNTVIQNVRRATRLPEPHFESIPVQRLLDDTLPLVEPVIQKSKVELLVQIAPDTPPLYVDNYRVQTALFNLIQ
NALEAMPHGGKIFVSARVSNDRQAIAVTVQDTGPGIPPSMMGKICEPFFSTHPGEGIRGLGLAIVQDIVKAHAGSIDIQS
QPHKGTCITLFFPIACRLQEIPG
>Mature_502_residues
TLKTRLMIITLFGLAITMALWGWIQLSVLDRILREQQYKRLDNIAETLSAFYQRFPSARGLSALDTALKDHIQTDVRLAR
IDIISLSGDDVDYIAGAGRVPYEWPENLIIRTDGKMEKHHYALQTEAGSAIGLLYPVHPEKGSSAKTMIGVILFSQSDME
ILSRARWMLFFSTLGLLVIILLVMAVGYDFLIGRPLRRIIETIDHFRTGKQASRIPIDRSDEWGQLADHFNVMAGEIENF
IAQNQELNRGLELRVKEATLKVVQLQRQVNQLQQLKALGYLTATLAHDLGTPLHSIAGMTQLLLERDEWPPDTARKLELI
LQQTQRLNTVIQNVRRATRLPEPHFESIPVQRLLDDTLPLVEPVIQKSKVELLVQIAPDTPPLYVDNYRVQTALFNLIQN
ALEAMPHGGKIFVSARVSNDRQAIAVTVQDTGPGIPPSMMGKICEPFFSTHPGEGIRGLGLAIVQDIVKAHAGSIDIQSQ
PHKGTCITLFFPIACRLQEIPG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=230, Percent_Identity=31.304347826087, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=30.1310043668122, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789149, Length=375, Percent_Identity=25.8666666666667, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081816, Length=358, Percent_Identity=26.8156424581006, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790346, Length=280, Percent_Identity=27.8571428571429, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790300, Length=243, Percent_Identity=29.2181069958848, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI145693157, Length=252, Percent_Identity=25.3968253968254, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786600, Length=259, Percent_Identity=27.4131274131274, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788393, Length=316, Percent_Identity=24.3670886075949, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=212, Percent_Identity=27.8301886792453, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786912, Length=234, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994928, Length=223, Percent_Identity=26.0089686098655, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56356; Mature: 56225

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKTRLMIITLFGLAITMALWGWIQLSVLDRILREQQYKRLDNIAETLSAFYQRFPSAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GLSALDTALKDHIQTDVRLARIDIISLSGDDVDYIAGAGRVPYEWPENLIIRTDGKMEKH
CHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHCCCCCCCCCCCCCEEEECCCCCHHH
HYALQTEAGSAIGLLYPVHPEKGSSAKTMIGVILFSQSDMEILSRARWMLFFSTLGLLVI
HEEEEECCCCEEEEEEECCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
ILLVMAVGYDFLIGRPLRRIIETIDHFRTGKQASRIPIDRSDEWGQLADHFNVMAGEIEN
HHHHHHHCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FIAQNQELNRGLELRVKEATLKVVQLQRQVNQLQQLKALGYLTATLAHDLGTPLHSIAGM
HHHCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TQLLLERDEWPPDTARKLELILQQTQRLNTVIQNVRRATRLPEPHFESIPVQRLLDDTLP
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LVEPVIQKSKVELLVQIAPDTPPLYVDNYRVQTALFNLIQNALEAMPHGGKIFVSARVSN
HHHHHHHHHHHEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECC
DRQAIAVTVQDTGPGIPPSMMGKICEPFFSTHPGEGIRGLGLAIVQDIVKAHAGSIDIQS
CCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
QPHKGTCITLFFPIACRLQEIPG
CCCCCCEEEEEEHHHHHHHCCCC
>Mature Secondary Structure 
TLKTRLMIITLFGLAITMALWGWIQLSVLDRILREQQYKRLDNIAETLSAFYQRFPSAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GLSALDTALKDHIQTDVRLARIDIISLSGDDVDYIAGAGRVPYEWPENLIIRTDGKMEKH
CHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHCCCCCCCCCCCCCEEEECCCCCHHH
HYALQTEAGSAIGLLYPVHPEKGSSAKTMIGVILFSQSDMEILSRARWMLFFSTLGLLVI
HEEEEECCCCEEEEEEECCCCCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
ILLVMAVGYDFLIGRPLRRIIETIDHFRTGKQASRIPIDRSDEWGQLADHFNVMAGEIEN
HHHHHHHCHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FIAQNQELNRGLELRVKEATLKVVQLQRQVNQLQQLKALGYLTATLAHDLGTPLHSIAGM
HHHCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TQLLLERDEWPPDTARKLELILQQTQRLNTVIQNVRRATRLPEPHFESIPVQRLLDDTLP
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
LVEPVIQKSKVELLVQIAPDTPPLYVDNYRVQTALFNLIQNALEAMPHGGKIFVSARVSN
HHHHHHHHHHHEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECC
DRQAIAVTVQDTGPGIPPSMMGKICEPFFSTHPGEGIRGLGLAIVQDIVKAHAGSIDIQS
CCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
QPHKGTCITLFFPIACRLQEIPG
CCCCCCEEEEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]