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The map label for this gene is hipO [H]

Identifier: 85860408

GI number: 85860408

Start: 2576176

End: 2577501

Strand: Direct

Name: hipO [H]

Synonym: SYN_00083

Alternate gene names: 85860408

Gene position: 2576176-2577501 (Clockwise)

Preceding gene: 85860407

Following gene: 85860409

Centisome position: 81.03

GC content: 61.69

Gene sequence:

>1326_bases
TTGGCGCAATGCGCCAACTGGCATGGATCTGAAGCTAAGATCACGGAAAGGAGCCTCATGTCCCCCGGCAAACTCACAAA
GCCCGAATCTCATGACCCGTTGAAAACGCTTTCCGCGATTCAGGAAAAGGACTATCCTTATCTTGAAAGTCTCTATCAGG
ATCTCCACCGCCATCCGGAACTTTCCGGAAGGGAAGAGAAAACGTCCAGGCGGATGACCGTGGAGATGGAGCAGGCAGGT
TTCCGGGCTACGCCGGGCATCGGCGGTAACGGGGTGGTGGGGATGCTGGAAAACGGTCCCGGTCCGGTCGTCATGGTCCG
GGCGGACATGGACGCCCTGCCGATTGAGGAGAAAACCGGTCTCCCTTACGCCAGCCGGGTCAGGACCCTGATGGAATCAG
GGCAGGAGGTCGGCGTGATGCATGCCTGCGGCCACGATGTCCACATGACCGTCCTGATTGGGGCGGCACGGCTGCTGGCG
CGATTCCGCAATTGCTGGCAGGGAACCCTGCTTCTGGTCGCTCAGCCTGCCGAGGAAAACGCCGCCGGCGCGGCGGCCAT
GATCGCCGACGGCCTTTTCATCCGCTTTCCCCGTCCCGATTTCGCTCTTGGCCTCCATGTTCTCCCCGGGCCGGCAGGCG
CCGTTCTTTATCACGAAGGTTTCTGGTTCGCCGGCTCGACAACTCTCGAGCTGACCGTGTACGGGAAAGGAGGACATGCC
GCCCGGCCTCATGAGACGCGGGACCCCGTCGTTCTGGCTGCCCAGATGATCCTCGCGTTTCAGACGGTGGTCAGCCGGGA
AACCGATCCGATGGAACCGGCGGTGCTCACCGTATCCTCTTTTCATGCGGGAAACCGGGACAACGTCATTCCGGAAACGG
CCACTCTCCAGATCAGCCTCCGCGCGTTCAGCCGCGAACAGCATGAGCGGATGATTTCCGCCCTGAAACGGATCGCCGAG
GGCATCGCCAGGACGGGCGGCATCCCCGAGGATCAGCTTCCCCGACTGTCAGTCAGATCATCCACGCCGGTCCTCTACAA
CGATCCTCGGCTCACCCGCAGACTCGTCCGCCGGTTCCGCCGGACTTTCGGCCGTTCCCGGGTCGCTGAAGTGCCGGCAA
TGACGGCCTCGGAGGATTTCTCCTGTTTCGGGCAGGCGGGAATTCCTCTCTGCTATTTCGGACTGGGAATGGCGGATCCC
CAGGGAGAAAGCCCTCCCCTTCACAGTCCTTACATGGCTCCTCTCCCGGAACCGACGATTTCGACCGGAGTTGCCGCCAT
GACCGCAGCCGTTCTGGAACTGCTCCCGGCTCTTGATCAAAGCTGA

Upstream 100 bases:

>100_bases
GGAAGCTGGCTGGGCGCCATCCTGGCGAATCATCTTTCCGGTCCGCATTTGAAACTGGCCTTTGGAATTTTTGTAGCGGG
TCTCGGCATCTACCTGATCA

Downstream 100 bases:

>100_bases
TGCTCAGGCGGCAAGGCAGAGGCAGAGAAGGTTCAATTTTTAAGGTTCAAGATTCTATGTTCTACGTTTCAGGTCAGGAT
TTCCAGGCGAAAAACTTAGA

Product: metal-dependent amidase/aminoacylase/carboxypeptidase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAQCANWHGSEAKITERSLMSPGKLTKPESHDPLKTLSAIQEKDYPYLESLYQDLHRHPELSGREEKTSRRMTVEMEQAG
FRATPGIGGNGVVGMLENGPGPVVMVRADMDALPIEEKTGLPYASRVRTLMESGQEVGVMHACGHDVHMTVLIGAARLLA
RFRNCWQGTLLLVAQPAEENAAGAAAMIADGLFIRFPRPDFALGLHVLPGPAGAVLYHEGFWFAGSTTLELTVYGKGGHA
ARPHETRDPVVLAAQMILAFQTVVSRETDPMEPAVLTVSSFHAGNRDNVIPETATLQISLRAFSREQHERMISALKRIAE
GIARTGGIPEDQLPRLSVRSSTPVLYNDPRLTRRLVRRFRRTFGRSRVAEVPAMTASEDFSCFGQAGIPLCYFGLGMADP
QGESPPLHSPYMAPLPEPTISTGVAAMTAAVLELLPALDQS

Sequences:

>Translated_441_residues
MAQCANWHGSEAKITERSLMSPGKLTKPESHDPLKTLSAIQEKDYPYLESLYQDLHRHPELSGREEKTSRRMTVEMEQAG
FRATPGIGGNGVVGMLENGPGPVVMVRADMDALPIEEKTGLPYASRVRTLMESGQEVGVMHACGHDVHMTVLIGAARLLA
RFRNCWQGTLLLVAQPAEENAAGAAAMIADGLFIRFPRPDFALGLHVLPGPAGAVLYHEGFWFAGSTTLELTVYGKGGHA
ARPHETRDPVVLAAQMILAFQTVVSRETDPMEPAVLTVSSFHAGNRDNVIPETATLQISLRAFSREQHERMISALKRIAE
GIARTGGIPEDQLPRLSVRSSTPVLYNDPRLTRRLVRRFRRTFGRSRVAEVPAMTASEDFSCFGQAGIPLCYFGLGMADP
QGESPPLHSPYMAPLPEPTISTGVAAMTAAVLELLPALDQS
>Mature_440_residues
AQCANWHGSEAKITERSLMSPGKLTKPESHDPLKTLSAIQEKDYPYLESLYQDLHRHPELSGREEKTSRRMTVEMEQAGF
RATPGIGGNGVVGMLENGPGPVVMVRADMDALPIEEKTGLPYASRVRTLMESGQEVGVMHACGHDVHMTVLIGAARLLAR
FRNCWQGTLLLVAQPAEENAAGAAAMIADGLFIRFPRPDFALGLHVLPGPAGAVLYHEGFWFAGSTTLELTVYGKGGHAA
RPHETRDPVVLAAQMILAFQTVVSRETDPMEPAVLTVSSFHAGNRDNVIPETATLQISLRAFSREQHERMISALKRIAEG
IARTGGIPEDQLPRLSVRSSTPVLYNDPRLTRRLVRRFRRTFGRSRVAEVPAMTASEDFSCFGQAGIPLCYFGLGMADPQ
GESPPLHSPYMAPLPEPTISTGVAAMTAAVLELLPALDQS

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=247, Percent_Identity=29.9595141700405, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 47950; Mature: 47818

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQCANWHGSEAKITERSLMSPGKLTKPESHDPLKTLSAIQEKDYPYLESLYQDLHRHPE
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCC
LSGREEKTSRRMTVEMEQAGFRATPGIGGNGVVGMLENGPGPVVMVRADMDALPIEEKTG
CCCCHHHHCCCEEEEHHHCCCEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCC
LPYASRVRTLMESGQEVGVMHACGHDVHMTVLIGAARLLARFRNCWQGTLLLVAQPAEEN
CCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
AAGAAAMIADGLFIRFPRPDFALGLHVLPGPAGAVLYHEGFWFAGSTTLELTVYGKGGHA
CCCHHHHHHCCEEEEECCCCCEEEEEECCCCCCEEEEECCEEEECCEEEEEEEEECCCCC
ARPHETRDPVVLAAQMILAFQTVVSRETDPMEPAVLTVSSFHAGNRDNVIPETATLQISL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEH
RAFSREQHERMISALKRIAEGIARTGGIPEDQLPRLSVRSSTPVLYNDPRLTRRLVRRFR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEECCHHHHHHHHHHHH
RTFGRSRVAEVPAMTASEDFSCFGQAGIPLCYFGLGMADPQGESPPLHSPYMAPLPEPTI
HHHCCCHHHHCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
STGVAAMTAAVLELLPALDQS
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AQCANWHGSEAKITERSLMSPGKLTKPESHDPLKTLSAIQEKDYPYLESLYQDLHRHPE
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCC
LSGREEKTSRRMTVEMEQAGFRATPGIGGNGVVGMLENGPGPVVMVRADMDALPIEEKTG
CCCCHHHHCCCEEEEHHHCCCEECCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCC
LPYASRVRTLMESGQEVGVMHACGHDVHMTVLIGAARLLARFRNCWQGTLLLVAQPAEEN
CCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
AAGAAAMIADGLFIRFPRPDFALGLHVLPGPAGAVLYHEGFWFAGSTTLELTVYGKGGHA
CCCHHHHHHCCEEEEECCCCCEEEEEECCCCCCEEEEECCEEEECCEEEEEEEEECCCCC
ARPHETRDPVVLAAQMILAFQTVVSRETDPMEPAVLTVSSFHAGNRDNVIPETATLQISL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEH
RAFSREQHERMISALKRIAEGIARTGGIPEDQLPRLSVRSSTPVLYNDPRLTRRLVRRFR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEECCHHHHHHHHHHHH
RTFGRSRVAEVPAMTASEDFSCFGQAGIPLCYFGLGMADPQGESPPLHSPYMAPLPEPTI
HHHCCCHHHHCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
STGVAAMTAAVLELLPALDQS
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]