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The map label for this gene is braC [H]

Identifier: 85860149

GI number: 85860149

Start: 2354929

End: 2356131

Strand: Direct

Name: braC [H]

Synonym: SYN_00789

Alternate gene names: 85860149

Gene position: 2354929-2356131 (Clockwise)

Preceding gene: 85860147

Following gene: 85860150

Centisome position: 74.07

GC content: 56.03

Gene sequence:

>1203_bases
ATGTTTCAGGAGGGCAGAATGAGTCGTTTCAGAAAATTTCATTCTTTCAGACTTTTATTTTCAGGATTGCTGATCCTGTT
TTTCATTGTTTCGTTTGCAGGATGCGGTAAAGACCAGGGGAAAGCGCCTGCGGGAGGAGAAAAGAAAGAAACCGCGGCAG
GAGCCATCCGGATCGGCGTCGCCGGCTCTCACAGCGGCGATCTGGCCTCCTACGGGCTGCCCACGGTCAAGGCGGCGGAA
CTGGTGGTTAAGGGCATCAATGCCAAAGGCGGCATTCTGGGCCGAAAGGTTGAACTGCTTGTCGAAGACGATGCCTGCAA
ACCGGAAATGGCGGCCAACATCGCGGCGAAACTGGTCTCCTCGAAGGTCGACGCCGTCATCGGTCACATCTGCAGCGGCG
CGACGAAAGCAGCCATCGGCATATACAAGGATGCCGGGATCATTGCCATATCCCCCTCGGCAACCCACCCGGAGCTGACC
CAGAGCGGGCAGTACCCGAACTTTTTCCGGACCATCGCCCCGGATGACGCCCAGGCGAGGCTGCAGGTGGATTTTGCCAT
CAACAGTCTGAAGGCCCGCAGGATCGCGGTTCTCCATGATAAGGGCGATTACGGCAAGGGCCTGGCCGAATACGCCAGAA
AATTCATCGAGTCATCGAACGCGGCTCAGGTTGTCCTCTTCGAAGGCGTAACCCCGGGGGCGGTGGACTATTCAGCAGTC
GTTCAGAAGATCAAAGGATCTGACGCCGATGCCGTTATTTATGGCGGCTATCACCCGGAGGCGTCATCCATTGTCATGCA
GATGCGCAAGAAAAACATGAAAACCTGGTTCATCTCCGATGACGGGGTTAAGGACATCACATTCATCAAGGTAGCCGGCT
CCTATGCCGAGGGCGTCTATGCCACCGGTCCCAGGGACACATCCTCCAATCCCCTGGCCATTGCCGCCAATGAAGCACAC
AAGAAAGCCTATGGTTCCGATCCCGGAGCCTTCTATCTGAATGCCTATGCGGCGACCCAGGCGCTGCTCAACGCCATCGA
GAAGGCCGGCTCCACGGATTCTGAAAAGATTAAACAGACCCTGCGTTCTCAGGACGTGGATACCCCCCTGGGGAAGATCC
GCTTTGACGAACGTGGGGATGCCCTGGGCGTGGGTTTTGCCATGTATCAAGTAAAAAACGGAGCCTACGTCGAGGTTAAA
TAA

Upstream 100 bases:

>100_bases
GGGTTGCAGCCCGTATTTTTCAGGACGGACTAAGTAGTAAGGATAGATTTCAAAACAGGAATCGACGGACAATCGCCATT
ACACATCAAACTCATAGAAT

Downstream 100 bases:

>100_bases
GTTCAATCAGTCAGGTAAAATGGACTATTTTCTGGAGCTTCTGGTCGGGGGGCTGACCCGCGGCAGTATTTACGCCCTCA
TCGCCCTCGGCTACACGATG

Product: branched-chain amino acid ABC transporter periplasmic protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MFQEGRMSRFRKFHSFRLLFSGLLILFFIVSFAGCGKDQGKAPAGGEKKETAAGAIRIGVAGSHSGDLASYGLPTVKAAE
LVVKGINAKGGILGRKVELLVEDDACKPEMAANIAAKLVSSKVDAVIGHICSGATKAAIGIYKDAGIIAISPSATHPELT
QSGQYPNFFRTIAPDDAQARLQVDFAINSLKARRIAVLHDKGDYGKGLAEYARKFIESSNAAQVVLFEGVTPGAVDYSAV
VQKIKGSDADAVIYGGYHPEASSIVMQMRKKNMKTWFISDDGVKDITFIKVAGSYAEGVYATGPRDTSSNPLAIAANEAH
KKAYGSDPGAFYLNAYAATQALLNAIEKAGSTDSEKIKQTLRSQDVDTPLGKIRFDERGDALGVGFAMYQVKNGAYVEVK

Sequences:

>Translated_400_residues
MFQEGRMSRFRKFHSFRLLFSGLLILFFIVSFAGCGKDQGKAPAGGEKKETAAGAIRIGVAGSHSGDLASYGLPTVKAAE
LVVKGINAKGGILGRKVELLVEDDACKPEMAANIAAKLVSSKVDAVIGHICSGATKAAIGIYKDAGIIAISPSATHPELT
QSGQYPNFFRTIAPDDAQARLQVDFAINSLKARRIAVLHDKGDYGKGLAEYARKFIESSNAAQVVLFEGVTPGAVDYSAV
VQKIKGSDADAVIYGGYHPEASSIVMQMRKKNMKTWFISDDGVKDITFIKVAGSYAEGVYATGPRDTSSNPLAIAANEAH
KKAYGSDPGAFYLNAYAATQALLNAIEKAGSTDSEKIKQTLRSQDVDTPLGKIRFDERGDALGVGFAMYQVKNGAYVEVK
>Mature_400_residues
MFQEGRMSRFRKFHSFRLLFSGLLILFFIVSFAGCGKDQGKAPAGGEKKETAAGAIRIGVAGSHSGDLASYGLPTVKAAE
LVVKGINAKGGILGRKVELLVEDDACKPEMAANIAAKLVSSKVDAVIGHICSGATKAAIGIYKDAGIIAISPSATHPELT
QSGQYPNFFRTIAPDDAQARLQVDFAINSLKARRIAVLHDKGDYGKGLAEYARKFIESSNAAQVVLFEGVTPGAVDYSAV
VQKIKGSDADAVIYGGYHPEASSIVMQMRKKNMKTWFISDDGVKDITFIKVAGSYAEGVYATGPRDTSSNPLAIAANEAH
KKAYGSDPGAFYLNAYAATQALLNAIEKAGSTDSEKIKQTLRSQDVDTPLGKIRFDERGDALGVGFAMYQVKNGAYVEVK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=334, Percent_Identity=38.622754491018, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789867, Length=335, Percent_Identity=37.3134328358209, Blast_Score=232, Evalue=3e-62,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42535; Mature: 42535

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQEGRMSRFRKFHSFRLLFSGLLILFFIVSFAGCGKDQGKAPAGGEKKETAAGAIRIGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCEEEEEE
AGSHSGDLASYGLPTVKAAELVVKGINAKGGILGRKVELLVEDDACKPEMAANIAAKLVS
CCCCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
SKVDAVIGHICSGATKAAIGIYKDAGIIAISPSATHPELTQSGQYPNFFRTIAPDDAQAR
HHHHHHHHHHHCCCHHHEEEEEECCCEEEECCCCCCCHHHCCCCCCCHHEECCCCCCCEE
LQVDFAINSLKARRIAVLHDKGDYGKGLAEYARKFIESSNAAQVVLFEGVTPGAVDYSAV
EEEEEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
VQKIKGSDADAVIYGGYHPEASSIVMQMRKKNMKTWFISDDGVKDITFIKVAGSYAEGVY
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCCCCEE
ATGPRDTSSNPLAIAANEAHKKAYGSDPGAFYLNAYAATQALLNAIEKAGSTDSEKIKQT
ECCCCCCCCCCEEEEECHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHH
LRSQDVDTPLGKIRFDERGDALGVGFAMYQVKNGAYVEVK
HHHCCCCCCHHCEEECCCCCEEEEEEEEEEECCCCEEEEC
>Mature Secondary Structure
MFQEGRMSRFRKFHSFRLLFSGLLILFFIVSFAGCGKDQGKAPAGGEKKETAAGAIRIGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCEEEEEE
AGSHSGDLASYGLPTVKAAELVVKGINAKGGILGRKVELLVEDDACKPEMAANIAAKLVS
CCCCCCCHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
SKVDAVIGHICSGATKAAIGIYKDAGIIAISPSATHPELTQSGQYPNFFRTIAPDDAQAR
HHHHHHHHHHHCCCHHHEEEEEECCCEEEECCCCCCCHHHCCCCCCCHHEECCCCCCCEE
LQVDFAINSLKARRIAVLHDKGDYGKGLAEYARKFIESSNAAQVVLFEGVTPGAVDYSAV
EEEEEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
VQKIKGSDADAVIYGGYHPEASSIVMQMRKKNMKTWFISDDGVKDITFIKVAGSYAEGVY
HHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCCCCEE
ATGPRDTSSNPLAIAANEAHKKAYGSDPGAFYLNAYAATQALLNAIEKAGSTDSEKIKQT
ECCCCCCCCCCEEEEECHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHH
LRSQDVDTPLGKIRFDERGDALGVGFAMYQVKNGAYVEVK
HHHCCCCCCHHCEEECCCCCEEEEEEEEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]