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The map label for this gene is buk1 [H]

Identifier: 85859909

GI number: 85859909

Start: 2143385

End: 2144473

Strand: Direct

Name: buk1 [H]

Synonym: SYN_03090

Alternate gene names: 85859909

Gene position: 2143385-2144473 (Clockwise)

Preceding gene: 85859908

Following gene: 85859910

Centisome position: 67.42

GC content: 58.13

Gene sequence:

>1089_bases
ATGAATAAGGCCTGTTCCCTGCTTGTGATCAACGTCGGTTCGACATCGACAAAAGCGGCCTGGTTTCAGGGAAGAGAACC
GGCGGCCCTGGAAACGATCCGTTATCAAAGCGAAGATCTGGCCCCCTATGCCTCTCTGCGCGAGCAGTTGCCTCTTCGGG
AAAAGGGGCTCCTCGATTTTCTGAAAAAGAATTCACTCGATCCGGAAGCGGTCGATCTGTTCATAAGCCGCGGCGGTCTG
GGCAATCCGGCGCCGGCAGGCGTTTACCGGATTGACGAGGTCATGTGCGAGGACCTGCTGGAAGGAAGATACGGGAAACA
TCCTTCGGCTCTTGGGCCGGCGATGGCTCTGAACCTCTCGAAACGATACGGAAAACCGGCCATTGTCGTCGATCCGCCGA
GTACCGATGAATTTCATCCTCTGGCCAGGATTTCGGGCCTTCCGGAAATAGAGCGCAAGAGCGTTTTTCACGCCCTGAAC
CAGAAGGCCGCCGCACGGCGCCTCGCTTCGGACCTCGATAAAAGATATGAAGCGCTGAACCTCATTGTTGCCCACCTGGG
CGGCGGCATCACCATCGGCGCCCATCGCAACGGCAAAGTGATCGATTGCACCCATGGCCTGGGAGAGGGTCCCCTGACGC
CGGAGCGAGCCGGCGCCCTGCCGACGATGGATCTGATCGACCTGGTTTTTTCTGGAAATAAGGACAGGAAAACGATTTCG
GATGCACTTGTGGGAAAAGGGGGGCTCTGCGCCTATCTGGGAACGACCGACGCAACCGAAGTCCTGGGCAAGATCCTGGC
GGGAGATGAAAGGGCGAAGTTGATTTTTGAAGCCATGGCTTATCAGATCGCCAAGGAGATCGGCGCCATGGCCACCGTTC
TGGAAGGGCGAATCGATGGAATCGTCCTGACGGGCGGGCTGGCCAATGTGGAGATGCTGACGGACTGGATCCGGGGGCGG
GTTCAATTCATCGCCCCCGTCTTCGTCTATCCCGGCGAGGACGAAATGGCAGCCCTTGCCGAAGGCGGCCTGAGGGTGCT
GCTGAAGGAAGAAGACGTGAAGAAGTATCAGCGGGAGAGCGGATCGTAA

Upstream 100 bases:

>100_bases
GCGAAAGTGCCGGTCATCCCGGATGTTCCTTTCATTTCCGATGAGGAAAGGGCGGCGGAGCTCGCCCTCGCTGTTCTGAT
GACCCGCAAAGGAGGAAACC

Downstream 100 bases:

>100_bases
GGGTCATAAGGTTTCTGAAGAACAGGCTAAGACGGACGATTGCTGGTTTTTTCCCAAGTCGGGAAGGGTTTTCGATCCGA
CTCGTAGTCCGCAGTCGCCG

Product: butyrate kinase

Products: NA

Alternate protein names: BK 1; Branched-chain carboxylic acid kinase 1 [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MNKACSLLVINVGSTSTKAAWFQGREPAALETIRYQSEDLAPYASLREQLPLREKGLLDFLKKNSLDPEAVDLFISRGGL
GNPAPAGVYRIDEVMCEDLLEGRYGKHPSALGPAMALNLSKRYGKPAIVVDPPSTDEFHPLARISGLPEIERKSVFHALN
QKAAARRLASDLDKRYEALNLIVAHLGGGITIGAHRNGKVIDCTHGLGEGPLTPERAGALPTMDLIDLVFSGNKDRKTIS
DALVGKGGLCAYLGTTDATEVLGKILAGDERAKLIFEAMAYQIAKEIGAMATVLEGRIDGIVLTGGLANVEMLTDWIRGR
VQFIAPVFVYPGEDEMAALAEGGLRVLLKEEDVKKYQRESGS

Sequences:

>Translated_362_residues
MNKACSLLVINVGSTSTKAAWFQGREPAALETIRYQSEDLAPYASLREQLPLREKGLLDFLKKNSLDPEAVDLFISRGGL
GNPAPAGVYRIDEVMCEDLLEGRYGKHPSALGPAMALNLSKRYGKPAIVVDPPSTDEFHPLARISGLPEIERKSVFHALN
QKAAARRLASDLDKRYEALNLIVAHLGGGITIGAHRNGKVIDCTHGLGEGPLTPERAGALPTMDLIDLVFSGNKDRKTIS
DALVGKGGLCAYLGTTDATEVLGKILAGDERAKLIFEAMAYQIAKEIGAMATVLEGRIDGIVLTGGLANVEMLTDWIRGR
VQFIAPVFVYPGEDEMAALAEGGLRVLLKEEDVKKYQRESGS
>Mature_362_residues
MNKACSLLVINVGSTSTKAAWFQGREPAALETIRYQSEDLAPYASLREQLPLREKGLLDFLKKNSLDPEAVDLFISRGGL
GNPAPAGVYRIDEVMCEDLLEGRYGKHPSALGPAMALNLSKRYGKPAIVVDPPSTDEFHPLARISGLPEIERKSVFHALN
QKAAARRLASDLDKRYEALNLIVAHLGGGITIGAHRNGKVIDCTHGLGEGPLTPERAGALPTMDLIDLVFSGNKDRKTIS
DALVGKGGLCAYLGTTDATEVLGKILAGDERAKLIFEAMAYQIAKEIGAMATVLEGRIDGIVLTGGLANVEMLTDWIRGR
VQFIAPVFVYPGEDEMAALAEGGLRVLLKEEDVKKYQRESGS

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=173, Percent_Identity=28.9017341040462, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693184, Length=188, Percent_Identity=29.7872340425532, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 39007; Mature: 39007

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKACSLLVINVGSTSTKAAWFQGREPAALETIRYQSEDLAPYASLREQLPLREKGLLDF
CCCCEEEEEEECCCCCCCHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHH
LKKNSLDPEAVDLFISRGGLGNPAPAGVYRIDEVMCEDLLEGRYGKHPSALGPAMALNLS
HHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEECH
KRYGKPAIVVDPPSTDEFHPLARISGLPEIERKSVFHALNQKAAARRLASDLDKRYEALN
HHCCCCEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIVAHLGGGITIGAHRNGKVIDCTHGLGEGPLTPERAGALPTMDLIDLVFSGNKDRKTIS
HHHHHHCCCEEEECCCCCCEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHH
DALVGKGGLCAYLGTTDATEVLGKILAGDERAKLIFEAMAYQIAKEIGAMATVLEGRIDG
HHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IVLTGGLANVEMLTDWIRGRVQFIAPVFVYPGEDEMAALAEGGLRVLLKEEDVKKYQRES
EEEECCCCHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHCCCEEEEEEHHHHHHHHHHC
GS
CC
>Mature Secondary Structure
MNKACSLLVINVGSTSTKAAWFQGREPAALETIRYQSEDLAPYASLREQLPLREKGLLDF
CCCCEEEEEEECCCCCCCHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHH
LKKNSLDPEAVDLFISRGGLGNPAPAGVYRIDEVMCEDLLEGRYGKHPSALGPAMALNLS
HHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHEECH
KRYGKPAIVVDPPSTDEFHPLARISGLPEIERKSVFHALNQKAAARRLASDLDKRYEALN
HHCCCCEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIVAHLGGGITIGAHRNGKVIDCTHGLGEGPLTPERAGALPTMDLIDLVFSGNKDRKTIS
HHHHHHCCCEEEECCCCCCEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCHHHHH
DALVGKGGLCAYLGTTDATEVLGKILAGDERAKLIFEAMAYQIAKEIGAMATVLEGRIDG
HHHHCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IVLTGGLANVEMLTDWIRGRVQFIAPVFVYPGEDEMAALAEGGLRVLLKEEDVKKYQRES
EEEECCCCHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHCCCEEEEEEHHHHHHHHHHC
GS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]