Click here to switch to the map view.

The map label for this gene is mscL

Identifier: 85859712

GI number: 85859712

Start: 1944561

End: 1945019

Strand: Direct

Name: mscL

Synonym: SYN_01974

Alternate gene names: 85859712

Gene position: 1944561-1945019 (Clockwise)

Preceding gene: 85859711

Following gene: 85859714

Centisome position: 61.16

GC content: 49.89

Gene sequence:

>459_bases
ATGTTCAAGGAATTCAAGGAGTTTGCATTAAAAGGAAACGTTGTGGATATGGCCGTGGGTATCATTCTGGGTGTTGCCTT
CGGCGCCATCGTGAAATCACTTGTCGATGATCTTCTCATGCCCGGCATCGGTATACTCCTGGGCTCTGCCGATTTTTCCA
ATCTGTTTCTCGTCATAAAAGAAGGCGCAACCCCCGGACCTTTCACCACCCTGGCTGACGCGCAGAAAGCCGGCGCAGTC
ACGATTAATTATGGACTTTTTATCAATACCATCGTTAATTTTATTATTGTAGCTTTCGCCTTGTTTCTGGTTATCCGCAA
CATCAACCAACTCCGGAGAATGACGGAAAAGCCCCCTGTCGAAGAGGCGCCCACAACCAAAGACTGTCCCTACTGCCTCT
CCGCCATCCCCCTCAAAGCCACGCGCTGCCCGAACTGCACATCTGAACTGAAGGGTTAA

Upstream 100 bases:

>100_bases
TGTACCTTTAAGCACAGCGGCCTTTTTAGACAGCAGATCTTTATAATGCAGATGATGCTGAATTTTTTTAAAAATTTATA
TGAATAAGGAGGAATGAACG

Downstream 100 bases:

>100_bases
CTCCACACGTTGTGGTGCGTATGTCCGTCCACCAAAGGCGGCATACGCATCACCGAGAAAAACATTCAGAACACGCAACG
TTAATAGTTGCTGTTATCCA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MFKEFKEFALKGNVVDMAVGIILGVAFGAIVKSLVDDLLMPGIGILLGSADFSNLFLVIKEGATPGPFTTLADAQKAGAV
TINYGLFINTIVNFIIVAFALFLVIRNINQLRRMTEKPPVEEAPTTKDCPYCLSAIPLKATRCPNCTSELKG

Sequences:

>Translated_152_residues
MFKEFKEFALKGNVVDMAVGIILGVAFGAIVKSLVDDLLMPGIGILLGSADFSNLFLVIKEGATPGPFTTLADAQKAGAV
TINYGLFINTIVNFIIVAFALFLVIRNINQLRRMTEKPPVEEAPTTKDCPYCLSAIPLKATRCPNCTSELKG
>Mature_152_residues
MFKEFKEFALKGNVVDMAVGIILGVAFGAIVKSLVDDLLMPGIGILLGSADFSNLFLVIKEGATPGPFTTLADAQKAGAV
TINYGLFINTIVNFIIVAFALFLVIRNINQLRRMTEKPPVEEAPTTKDCPYCLSAIPLKATRCPNCTSELKG

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=130, Percent_Identity=46.1538461538462, Blast_Score=114, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_SYNAS (Q2LUI9)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461914.1
- ProteinModelPortal:   Q2LUI9
- SMR:   Q2LUI9
- STRING:   Q2LUI9
- GeneID:   3883915
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_01974
- NMPDR:   fig|56780.10.peg.1856
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- PhylomeDB:   Q2LUI9
- ProtClustDB:   PRK00567
- BioCyc:   SACI56780:SYN_01974-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 16340; Mature: 16340

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15dc72b0)-; HASH(0x16a5a7dc)-; HASH(0x16467508)-;

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEFKEFALKGNVVDMAVGIILGVAFGAIVKSLVDDLLMPGIGILLGSADFSNLFLVIK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEE
EGATPGPFTTLADAQKAGAVTINYGLFINTIVNFIIVAFALFLVIRNINQLRRMTEKPPV
CCCCCCCCHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EEAPTTKDCPYCLSAIPLKATRCPNCTSELKG
CCCCCCCCCCHHHHHCCCCCCCCCCCHHHCCH
>Mature Secondary Structure
MFKEFKEFALKGNVVDMAVGIILGVAFGAIVKSLVDDLLMPGIGILLGSADFSNLFLVIK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEE
EGATPGPFTTLADAQKAGAVTINYGLFINTIVNFIIVAFALFLVIRNINQLRRMTEKPPV
CCCCCCCCHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EEAPTTKDCPYCLSAIPLKATRCPNCTSELKG
CCCCCCCCCCHHHHHCCCCCCCCCCCHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA