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The map label for this gene is fliM [H]

Identifier: 85859177

GI number: 85859177

Start: 1355478

End: 1356479

Strand: Direct

Name: fliM [H]

Synonym: SYN_02837

Alternate gene names: 85859177

Gene position: 1355478-1356479 (Clockwise)

Preceding gene: 85859176

Following gene: 85859178

Centisome position: 42.63

GC content: 49.1

Gene sequence:

>1002_bases
ATGTCGCAGATTTTAACTCAGGAAGAAGTCGACGCTCTGCTGCGCGGCATTACAGGCGGTCAGATCGATACCGAAATTGA
TGAGGAAGAGGAGCCCTCCGATATCGTCATCTACGATCTGGCCAGTCAGGACCGGATCATCCGGGGGCGGATGCCCACCC
TGGAAATGACGAATGAGAAATTCGCACGCATGTTCCGGACGACCTTGTCTTCCATGCTGCGCAAGGTAGTGGCGGTCAAT
TCCATGTCGGTCGACATGATTAAATTCGGGGAATTTCTGAAAACCCTTCCCGTGCCGACGAGCCTGCATATTTTCCGGAT
GGAGCCGCTTCGAGGAAACGCGATCTTTGTGATGGAGTCCAAGGTCATTTTTACCCTTGTGGATGTACTTTTCGGTGGAG
CGGGGAAGGAACTGTTCAAGGTTGAAGGCAGAGAGTTTACAGCCATCGAGACGAATCTGATCAAAAAGGTGATTCTCAGT
GCCCTTTCCGACCTGGAAAAAGCCTGGAAGACCATGCATGACATTCGCGTGATTTATCAACGGTCTGAAATCAACCCCCA
ATTCGCGCAGATTGTTCCTCCCACGGATCTGGTTTTTGTCGTCGATTTTGAAATTGAGGTTGAATACATCACCGGAATTC
TCAAACTCTGCATTCCTTATTCAACGGTGGAGCCCATCCGGGAAAAATTACAGGCCGGATTCCAAAGTGAGCAGCTGGAA
GTGGACAAGGTATGGATGAAGCGGTTTCGCGATAATCTCATGCTGGCTCATCTCGATATCCTGATCGAGTTGGGCAGCAC
GCTGATATCCGCCAAAGATGTGGTCAATCTCAAGCCCGGTGACGTCATTACCCTGGATCAGTACTGCACCGATCCGGTGA
ATATCTTTGTCGAGGGCATGATGAAATTTAAGGGATATCCGGGAACATATAAAGGAAATCAAGCCGTAAGGATTTCGGAG
ATTGTTCAGGACAAAGAGGTTATGGATTATGGAACAGAATGA

Upstream 100 bases:

>100_bases
GCTTCGTGAGGATATTGCATTCAAGCTGAACAGCTTTTTATCAAAAGGCAGAATCATGCGCGTTTATTTTACAGAATTTG
TCATTCAGTAAGAGTTCTTC

Downstream 100 bases:

>100_bases
GATCGATGAACTTCTTAATGAAATCGATATGGATGAAAACTCGAATTCGAGTATGGGTTTGAGCGATGACGACAATTTCG
GTTGGGAAGATGTCAAGGAA

Product: flagellar motor switch protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSQILTQEEVDALLRGITGGQIDTEIDEEEEPSDIVIYDLASQDRIIRGRMPTLEMTNEKFARMFRTTLSSMLRKVVAVN
SMSVDMIKFGEFLKTLPVPTSLHIFRMEPLRGNAIFVMESKVIFTLVDVLFGGAGKELFKVEGREFTAIETNLIKKVILS
ALSDLEKAWKTMHDIRVIYQRSEINPQFAQIVPPTDLVFVVDFEIEVEYITGILKLCIPYSTVEPIREKLQAGFQSEQLE
VDKVWMKRFRDNLMLAHLDILIELGSTLISAKDVVNLKPGDVITLDQYCTDPVNIFVEGMMKFKGYPGTYKGNQAVRISE
IVQDKEVMDYGTE

Sequences:

>Translated_333_residues
MSQILTQEEVDALLRGITGGQIDTEIDEEEEPSDIVIYDLASQDRIIRGRMPTLEMTNEKFARMFRTTLSSMLRKVVAVN
SMSVDMIKFGEFLKTLPVPTSLHIFRMEPLRGNAIFVMESKVIFTLVDVLFGGAGKELFKVEGREFTAIETNLIKKVILS
ALSDLEKAWKTMHDIRVIYQRSEINPQFAQIVPPTDLVFVVDFEIEVEYITGILKLCIPYSTVEPIREKLQAGFQSEQLE
VDKVWMKRFRDNLMLAHLDILIELGSTLISAKDVVNLKPGDVITLDQYCTDPVNIFVEGMMKFKGYPGTYKGNQAVRISE
IVQDKEVMDYGTE
>Mature_332_residues
SQILTQEEVDALLRGITGGQIDTEIDEEEEPSDIVIYDLASQDRIIRGRMPTLEMTNEKFARMFRTTLSSMLRKVVAVNS
MSVDMIKFGEFLKTLPVPTSLHIFRMEPLRGNAIFVMESKVIFTLVDVLFGGAGKELFKVEGREFTAIETNLIKKVILSA
LSDLEKAWKTMHDIRVIYQRSEINPQFAQIVPPTDLVFVVDFEIEVEYITGILKLCIPYSTVEPIREKLQAGFQSEQLEV
DKVWMKRFRDNLMLAHLDILIELGSTLISAKDVVNLKPGDVITLDQYCTDPVNIFVEGMMKFKGYPGTYKGNQAVRISEI
VQDKEVMDYGTE

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=326, Percent_Identity=33.1288343558282, Blast_Score=191, Evalue=5e-50,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37893; Mature: 37761

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQILTQEEVDALLRGITGGQIDTEIDEEEEPSDIVIYDLASQDRIIRGRMPTLEMTNEK
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCHHHHHHH
FARMFRTTLSSMLRKVVAVNSMSVDMIKFGEFLKTLPVPTSLHIFRMEPLRGNAIFVMES
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEC
KVIFTLVDVLFGGAGKELFKVEGREFTAIETNLIKKVILSALSDLEKAWKTMHDIRVIYQ
HHHHHHHHHHHCCCCCHHEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSEINPQFAQIVPPTDLVFVVDFEIEVEYITGILKLCIPYSTVEPIREKLQAGFQSEQLE
HCCCCCHHHEECCCCCEEEEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCH
VDKVWMKRFRDNLMLAHLDILIELGSTLISAKDVVNLKPGDVITLDQYCTDPVNIFVEGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEHHHCCCHHHHHHHHH
MKFKGYPGTYKGNQAVRISEIVQDKEVMDYGTE
HHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQILTQEEVDALLRGITGGQIDTEIDEEEEPSDIVIYDLASQDRIIRGRMPTLEMTNEK
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCHHHHHHH
FARMFRTTLSSMLRKVVAVNSMSVDMIKFGEFLKTLPVPTSLHIFRMEPLRGNAIFVMES
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEC
KVIFTLVDVLFGGAGKELFKVEGREFTAIETNLIKKVILSALSDLEKAWKTMHDIRVIYQ
HHHHHHHHHHHCCCCCHHEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSEINPQFAQIVPPTDLVFVVDFEIEVEYITGILKLCIPYSTVEPIREKLQAGFQSEQLE
HCCCCCHHHEECCCCCEEEEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCH
VDKVWMKRFRDNLMLAHLDILIELGSTLISAKDVVNLKPGDVITLDQYCTDPVNIFVEGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEHHHCCCHHHHHHHHH
MKFKGYPGTYKGNQAVRISEIVQDKEVMDYGTE
HHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043; 7622217 [H]