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The map label for this gene is sppA [H]

Identifier: 85859094

GI number: 85859094

Start: 1272485

End: 1273414

Strand: Direct

Name: sppA [H]

Synonym: SYN_03198

Alternate gene names: 85859094

Gene position: 1272485-1273414 (Clockwise)

Preceding gene: 85859093

Following gene: 85859096

Centisome position: 40.02

GC content: 49.03

Gene sequence:

>930_bases
ATGAAACGACATCCGGTGATCTGGGGGATTTTCCTGCTGCTGATTCTCCTGATTGGGTTTTTTTCCCTGATTTATGGGGT
TACATCCATCACCGGGATGCGGGACGGATTTACTACTGCGGACAAAATCGGCATTGCTCCGATCGACGGTGTCATCAAAG
ATTCCACGGATGTTGTGAAGAATCTGCAGGAATTCGGTAAAGACGATTCCGTGAAGGGTGTTGTTCTGCGCATTAATTCT
CCGGGGGGGGGCGTTGCCCCTTCTCAGGAGATTTACGAAGCAGTCATGGAACTCCGCAAGAAAAAAAGAGTGCTTGTTTC
CATGAGTTCTGTGGCTGCATCCGGCGGATATCTGATTGCCTGTGCCGGAGAAAAGATTATCGCCAATCCCGGCACATTGA
CGGGGAGCGTTTCCGCAATCATGTATTTTACGAATGCGGAAGAATTGATGAAAAAGGTGGGGGTGAAGGCTTCCACGATA
AAAAGTGGAAAATATAAGGATATCGGATCTCCGATCCGGGAAATGACCGGAGAGGAAAAGGCCCTGCTCCAAGGTCTTGT
GGATGATATTTACATCCAGTTCCTCGATGTAGTCGTCAGGAACCGGCGCATTTCCGAGGAGGCATTAAAGCAGATCGCCG
ATGGCAGAATTTTTACAGGGAAACAGGCTCAGCAATTGGGGCTTGTGGACTATCTCGGCGATATGGAATATGCGGTCCGG
ATGCTTGCAAAAATGGTCGGAATTTCGGGCGAGCCGGTCGTTGTTTATCCCCAGAAAAAATATGAATCCATGCTTGATTA
TTTCTTTGAACGGGCGGGTTCCACAATGGCCCATGTGTTTTTCAGGGATCAGACCATTCCTTATGGCATTCATTATCTCG
TTGATTCTTACGGCTTGCGGATGTTGGCGCCTGCGGGCATGGAGCAGTGA

Upstream 100 bases:

>100_bases
CTTAATAATCGTGAAAACGTGGGGTCCAGTCTGGGAAAGGCCCTGGCGGATCTGGACGTAAAACTAACGGATCCGAACAA
CTGATTCTAGGCGCGATTCT

Downstream 100 bases:

>100_bases
CATTGGATTTAGGCCGAAAAACTCACCGGATTTCTAAGCAATACCGCCGTTATAGATTCCTCTTCCATCAGGATTCTTCT
CTCGAATAAAGAATCCAGAG

Product: signal peptide peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKRHPVIWGIFLLLILLIGFFSLIYGVTSITGMRDGFTTADKIGIAPIDGVIKDSTDVVKNLQEFGKDDSVKGVVLRINS
PGGGVAPSQEIYEAVMELRKKKRVLVSMSSVAASGGYLIACAGEKIIANPGTLTGSVSAIMYFTNAEELMKKVGVKASTI
KSGKYKDIGSPIREMTGEEKALLQGLVDDIYIQFLDVVVRNRRISEEALKQIADGRIFTGKQAQQLGLVDYLGDMEYAVR
MLAKMVGISGEPVVVYPQKKYESMLDYFFERAGSTMAHVFFRDQTIPYGIHYLVDSYGLRMLAPAGMEQ

Sequences:

>Translated_309_residues
MKRHPVIWGIFLLLILLIGFFSLIYGVTSITGMRDGFTTADKIGIAPIDGVIKDSTDVVKNLQEFGKDDSVKGVVLRINS
PGGGVAPSQEIYEAVMELRKKKRVLVSMSSVAASGGYLIACAGEKIIANPGTLTGSVSAIMYFTNAEELMKKVGVKASTI
KSGKYKDIGSPIREMTGEEKALLQGLVDDIYIQFLDVVVRNRRISEEALKQIADGRIFTGKQAQQLGLVDYLGDMEYAVR
MLAKMVGISGEPVVVYPQKKYESMLDYFFERAGSTMAHVFFRDQTIPYGIHYLVDSYGLRMLAPAGMEQ
>Mature_309_residues
MKRHPVIWGIFLLLILLIGFFSLIYGVTSITGMRDGFTTADKIGIAPIDGVIKDSTDVVKNLQEFGKDDSVKGVVLRINS
PGGGVAPSQEIYEAVMELRKKKRVLVSMSSVAASGGYLIACAGEKIIANPGTLTGSVSAIMYFTNAEELMKKVGVKASTI
KSGKYKDIGSPIREMTGEEKALLQGLVDDIYIQFLDVVVRNRRISEEALKQIADGRIFTGKQAQQLGLVDYLGDMEYAVR
MLAKMVGISGEPVVVYPQKKYESMLDYFFERAGSTMAHVFFRDQTIPYGIHYLVDSYGLRMLAPAGMEQ

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=220, Percent_Identity=33.6363636363636, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787527, Length=215, Percent_Identity=28.8372093023256, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 33934; Mature: 33934

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRHPVIWGIFLLLILLIGFFSLIYGVTSITGMRDGFTTADKIGIAPIDGVIKDSTDVVK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHCCCHHHHH
NLQEFGKDDSVKGVVLRINSPGGGVAPSQEIYEAVMELRKKKRVLVSMSSVAASGGYLIA
HHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CAGEKIIANPGTLTGSVSAIMYFTNAEELMKKVGVKASTIKSGKYKDIGSPIREMTGEEK
ECCCEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHCCHHH
ALLQGLVDDIYIQFLDVVVRNRRISEEALKQIADGRIFTGKQAQQLGLVDYLGDMEYAVR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEECCCHHHHHHHHHHHCCHHHHHH
MLAKMVGISGEPVVVYPQKKYESMLDYFFERAGSTMAHVFFRDQTIPYGIHYLVDSYGLR
HHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCC
MLAPAGMEQ
EECCCCCCC
>Mature Secondary Structure
MKRHPVIWGIFLLLILLIGFFSLIYGVTSITGMRDGFTTADKIGIAPIDGVIKDSTDVVK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHCCCHHHHH
NLQEFGKDDSVKGVVLRINSPGGGVAPSQEIYEAVMELRKKKRVLVSMSSVAASGGYLIA
HHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CAGEKIIANPGTLTGSVSAIMYFTNAEELMKKVGVKASTIKSGKYKDIGSPIREMTGEEK
ECCCEEECCCCCCCCCCEEEEEECCHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHCCHHH
ALLQGLVDDIYIQFLDVVVRNRRISEEALKQIADGRIFTGKQAQQLGLVDYLGDMEYAVR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEECCCHHHHHHHHHHHCCHHHHHH
MLAKMVGISGEPVVVYPQKKYESMLDYFFERAGSTMAHVFFRDQTIPYGIHYLVDSYGLR
HHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCC
MLAPAGMEQ
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]