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The map label for this gene is cheB2

Identifier: 85859039

GI number: 85859039

Start: 1214354

End: 1215385

Strand: Direct

Name: cheB2

Synonym: SYN_00964

Alternate gene names: 85859039

Gene position: 1214354-1215385 (Clockwise)

Preceding gene: 85859038

Following gene: 85859040

Centisome position: 38.2

GC content: 48.64

Gene sequence:

>1032_bases
ATGAGCAAGATAAAGGTCTTGGTTGTTGATGATTCGGCGATTGTCAGAAAAATTTTTACCGAAGAGCTCTCGAAATATCC
AGATATAGAAGTTGTCGGGTCGGCCCCGGATCCTTTTGTGGCCCGCGACAAAATTGTCCATCTGAAACCGGATGTGATTA
CGCTGGACATAGAAATGCCAAGGATGGATGGTTTGACCTTCCTGAAAAAGCTGATGAGACATTACCCTCTTCCCGCAATT
ATCGTCAGTTCCCTGACCCCCAAAGGGGGGAAACTGACCCTTGAGGCTATGGATATAGGAGCGGTCGATGCTATCGCTAA
GCCAGGAAGTTCATACTCTGTAGGTGATATGAGCGGACAGTTGGTTGAAAAGATCCGAGCGGCATCAGGGGCAAGAGTTG
TGAGGAAGAGCACACAGGAAATGTTTCAGCCCGGCTCACGTCTTTCCATAAGATCTCTTGCTGAAACTTCGAATAAAGTT
ATTGCCATTGGCGCATCGACAGGCGGGACGGAAGCTCTGAAAAACGTGCTTTCCAGGATGCCTCCCGATTCACCTGGAAT
TCTTGTAGTGCAGCATATGCCGGCGAACTTCACCACGGCCTTCGCGGAACGCTTGAACAGTCTTTGTCAAATTGGCGTTA
AAGAAGCCGCGGATAACGATTCGGTAACTCCCGGAACAGCGCTTATCGCACCGGGAAACTATCACATGATTCTACGGCGG
AGTGGCGCACGATATTATGTTCAAATAAAAATGGGTCCCATGGTGCATCATCAAAGGCCGGCTGTGGACGTTCTTTTTAA
ATCAACGGCTAAATATGCAGGGGCTAATGCCATCGGTGTCATTTTGACCGGGATGGGCGCCGATGGTGCTGCGGGACTTC
TGGAAATGAAGCAGATGGGAGCAGGCACGATCGCGCAGAATGAAAAATCCTGTATTGTTTATGGAATGCCCAAGGAAGCC
GTGAAAATAGGGGCGGTTGATAAAATCGTTCATCTTGATTCGATTGCTGATGAAATTGTGAGGATGGTGTAG

Upstream 100 bases:

>100_bases
AGATTGTCTACAATACCTTTCAGAATGAAGCCCTGATCTACAAGGTCAATACGCTTCGAAACAGTGACTGGTATCCATAT
GATGGGCAAGGCAGACAGGC

Downstream 100 bases:

>100_bases
CGATGAATTATGAAACCCTGTTGAAGTCAATTGATTGCATGGCGTCAGATTTTCTCTTTCTTGACAGCGGAGATATGGAT
GTGCCGACGACAGGAAAATT

Product: protein-glutamate methylesterase CheB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSKIKVLVVDDSAIVRKIFTEELSKYPDIEVVGSAPDPFVARDKIVHLKPDVITLDIEMPRMDGLTFLKKLMRHYPLPAI
IVSSLTPKGGKLTLEAMDIGAVDAIAKPGSSYSVGDMSGQLVEKIRAASGARVVRKSTQEMFQPGSRLSIRSLAETSNKV
IAIGASTGGTEALKNVLSRMPPDSPGILVVQHMPANFTTAFAERLNSLCQIGVKEAADNDSVTPGTALIAPGNYHMILRR
SGARYYVQIKMGPMVHHQRPAVDVLFKSTAKYAGANAIGVILTGMGADGAAGLLEMKQMGAGTIAQNEKSCIVYGMPKEA
VKIGAVDKIVHLDSIADEIVRMV

Sequences:

>Translated_343_residues
MSKIKVLVVDDSAIVRKIFTEELSKYPDIEVVGSAPDPFVARDKIVHLKPDVITLDIEMPRMDGLTFLKKLMRHYPLPAI
IVSSLTPKGGKLTLEAMDIGAVDAIAKPGSSYSVGDMSGQLVEKIRAASGARVVRKSTQEMFQPGSRLSIRSLAETSNKV
IAIGASTGGTEALKNVLSRMPPDSPGILVVQHMPANFTTAFAERLNSLCQIGVKEAADNDSVTPGTALIAPGNYHMILRR
SGARYYVQIKMGPMVHHQRPAVDVLFKSTAKYAGANAIGVILTGMGADGAAGLLEMKQMGAGTIAQNEKSCIVYGMPKEA
VKIGAVDKIVHLDSIADEIVRMV
>Mature_342_residues
SKIKVLVVDDSAIVRKIFTEELSKYPDIEVVGSAPDPFVARDKIVHLKPDVITLDIEMPRMDGLTFLKKLMRHYPLPAII
VSSLTPKGGKLTLEAMDIGAVDAIAKPGSSYSVGDMSGQLVEKIRAASGARVVRKSTQEMFQPGSRLSIRSLAETSNKVI
AIGASTGGTEALKNVLSRMPPDSPGILVVQHMPANFTTAFAERLNSLCQIGVKEAADNDSVTPGTALIAPGNYHMILRRS
GARYYVQIKMGPMVHHQRPAVDVLFKSTAKYAGANAIGVILTGMGADGAAGLLEMKQMGAGTIAQNEKSCIVYGMPKEAV
KIGAVDKIVHLDSIADEIVRMV

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=345, Percent_Identity=53.3333333333333, Blast_Score=364, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB2_SYNAS (Q2LSL3)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461241.1
- ProteinModelPortal:   Q2LSL3
- SMR:   Q2LSL3
- STRING:   Q2LSL3
- GeneID:   3882494
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_00964
- NMPDR:   fig|56780.10.peg.1175
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   DIPARMM
- PhylomeDB:   Q2LSL3
- ProtClustDB:   CLSK2397537
- BioCyc:   SACI56780:SYN_00964-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 36651; Mature: 36520

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 166-166 ACT_SITE 192-192 ACT_SITE 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIKVLVVDDSAIVRKIFTEELSKYPDIEVVGSAPDPFVARDKIVHLKPDVITLDIEMP
CCCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEECCCEEEEEEECC
RMDGLTFLKKLMRHYPLPAIIVSSLTPKGGKLTLEAMDIGAVDAIAKPGSSYSVGDMSGQ
CCCCHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCCCCCHH
LVEKIRAASGARVVRKSTQEMFQPGSRLSIRSLAETSNKVIAIGASTGGTEALKNVLSRM
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHC
PPDSPGILVVQHMPANFTTAFAERLNSLCQIGVKEAADNDSVTPGTALIAPGNYHMILRR
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEEECCCEEEEEEE
SGARYYVQIKMGPMVHHQRPAVDVLFKSTAKYAGANAIGVILTGMGADGAAGLLEMKQMG
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCC
AGTIAQNEKSCIVYGMPKEAVKIGAVDKIVHLDSIADEIVRMV
CCCCCCCCCCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SKIKVLVVDDSAIVRKIFTEELSKYPDIEVVGSAPDPFVARDKIVHLKPDVITLDIEMP
CCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEECCCEEEEEEECC
RMDGLTFLKKLMRHYPLPAIIVSSLTPKGGKLTLEAMDIGAVDAIAKPGSSYSVGDMSGQ
CCCCHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEECCCCHHHHCCCCCCCCCCCCCHH
LVEKIRAASGARVVRKSTQEMFQPGSRLSIRSLAETSNKVIAIGASTGGTEALKNVLSRM
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHC
PPDSPGILVVQHMPANFTTAFAERLNSLCQIGVKEAADNDSVTPGTALIAPGNYHMILRR
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEEECCCEEEEEEE
SGARYYVQIKMGPMVHHQRPAVDVLFKSTAKYAGANAIGVILTGMGADGAAGLLEMKQMG
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCC
AGTIAQNEKSCIVYGMPKEAVKIGAVDKIVHLDSIADEIVRMV
CCCCCCCCCCEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA