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The map label for this gene is ald [H]

Identifier: 85859023

GI number: 85859023

Start: 1201197

End: 1202426

Strand: Direct

Name: ald [H]

Synonym: SYN_00979

Alternate gene names: 85859023

Gene position: 1201197-1202426 (Clockwise)

Preceding gene: 85859021

Following gene: 85859026

Centisome position: 37.78

GC content: 54.88

Gene sequence:

>1230_bases
TTGACACGCCTTGAAACCTGTGGCAGTAACATACCCTGCTTGGTTTTCCTCAGGGCAGCGGCTGCCTTTTCACCGTATGA
ACGGAAGGGAAAACGGAATGAACGATTGAAGAGAGGCTCTATGCTGATTGGAGTTCCGAAAGAAATCAAGGATCATGAAT
ACCGCGTTGCCTTGACGCCGGGAAGTGTCGGGACGCTTGTCCGGGCGGGTCACAAAATTCTTGTGCAGTCTGGAGCAGGG
GAAGGCAGTGGATTTCCCGATGATGAGTATCGTTCATCGGGAGCCATTCTTTTTCCGGAAGCCGCCGACATATGGGAAGG
GGCCGGGATGGTCATGAAAGTGAAGGAGCCTCAGCCATCAGAGTATATCTATCTGAGGAATGATCTGATCCTTTTTACTT
ACCTCCATCTGGCCGCTGAACCGGACCTGACCCGTGTGCTTCTGGATTCCGGAACGGCTGCCATCGGTTACGAGACAGTT
CAGTTGGATAATGGAACGCTGCCCCTGCTCATGCCCATGTCGGAAGTTGCCGGCCGGATGTCTGCTCAGATTGTTGCTCA
CTATCTGGAGAAAGAAAACGGGGGGCGTGGAAAGCTTCTCGGTGGGGTGCCCGGCGTTCGTCCTGCGGATGTGGTGATCC
TCGGTGGAGGAATTGTCGGGAGCAACGCGGCCAAAATCGCGTTGGGAATGGGTGCACATGTAACCATCATCGACACCAGT
TCGGACCGCCTCCGGTATCTGGATGAGGTGCTTCACGGCAATCTGACGACTCTCGGTTCCAATGTGCAGAATATCGCTGA
TTCCGTCAATCGCGCCGACGGACTGGTTGGAAGCGTTCTCATCCCAGGGGCTAAGACTCCGAAACTCGTCAGCCGGGAAA
TGGTGGCCGCGATGAAGCCGGGAAGCGTCATTGTGGATGTTGCCATCGATCAGGGAGGCTGCGTGGAAACGGCCAGACCG
ACAAAGCACAGCGATCCCGTTTATATTGTGGATGAGGTGATCCACTATTGTGTGACCAACATGCCCGGCGCCGTCCCGCG
AACATCTACCTATGCACTGAACAATGCGACTCTTCCTTATGCGTTGAAAATTGCAGGATCAGGTCTGATGGATGCTCTTA
AAGCAGATATCTCCCTTGTCCGGGGCGTCAATACGCTGGGAGGCCGTCTGGCCAGCCGTCCTGTCGCAGAGGCTCATGGT
CTGGAGTATATCCCCCTTTTCAGGCGGTGA

Upstream 100 bases:

>100_bases
GTTGAATCTCCCCAAAAAGTTAGCGGGATATTTACCTCCTTATCTATATTTTGGCAAGTGAAAGGGTGCAAAGCAGATCT
CTTTATCCCTCATTATCCCT

Downstream 100 bases:

>100_bases
GCTGAAAAGCCGGGACATTAATCGGAATGACGGGGGGATTTACAAATGCATATTTTAAGTGACGATTAAAGAATTTTTCA
ATTTATTTTCCGTCATGACC

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTRLETCGSNIPCLVFLRAAAAFSPYERKGKRNERLKRGSMLIGVPKEIKDHEYRVALTPGSVGTLVRAGHKILVQSGAG
EGSGFPDDEYRSSGAILFPEAADIWEGAGMVMKVKEPQPSEYIYLRNDLILFTYLHLAAEPDLTRVLLDSGTAAIGYETV
QLDNGTLPLLMPMSEVAGRMSAQIVAHYLEKENGGRGKLLGGVPGVRPADVVILGGGIVGSNAAKIALGMGAHVTIIDTS
SDRLRYLDEVLHGNLTTLGSNVQNIADSVNRADGLVGSVLIPGAKTPKLVSREMVAAMKPGSVIVDVAIDQGGCVETARP
TKHSDPVYIVDEVIHYCVTNMPGAVPRTSTYALNNATLPYALKIAGSGLMDALKADISLVRGVNTLGGRLASRPVAEAHG
LEYIPLFRR

Sequences:

>Translated_409_residues
MTRLETCGSNIPCLVFLRAAAAFSPYERKGKRNERLKRGSMLIGVPKEIKDHEYRVALTPGSVGTLVRAGHKILVQSGAG
EGSGFPDDEYRSSGAILFPEAADIWEGAGMVMKVKEPQPSEYIYLRNDLILFTYLHLAAEPDLTRVLLDSGTAAIGYETV
QLDNGTLPLLMPMSEVAGRMSAQIVAHYLEKENGGRGKLLGGVPGVRPADVVILGGGIVGSNAAKIALGMGAHVTIIDTS
SDRLRYLDEVLHGNLTTLGSNVQNIADSVNRADGLVGSVLIPGAKTPKLVSREMVAAMKPGSVIVDVAIDQGGCVETARP
TKHSDPVYIVDEVIHYCVTNMPGAVPRTSTYALNNATLPYALKIAGSGLMDALKADISLVRGVNTLGGRLASRPVAEAHG
LEYIPLFRR
>Mature_408_residues
TRLETCGSNIPCLVFLRAAAAFSPYERKGKRNERLKRGSMLIGVPKEIKDHEYRVALTPGSVGTLVRAGHKILVQSGAGE
GSGFPDDEYRSSGAILFPEAADIWEGAGMVMKVKEPQPSEYIYLRNDLILFTYLHLAAEPDLTRVLLDSGTAAIGYETVQ
LDNGTLPLLMPMSEVAGRMSAQIVAHYLEKENGGRGKLLGGVPGVRPADVVILGGGIVGSNAAKIALGMGAHVTIIDTSS
DRLRYLDEVLHGNLTTLGSNVQNIADSVNRADGLVGSVLIPGAKTPKLVSREMVAAMKPGSVIVDVAIDQGGCVETARPT
KHSDPVYIVDEVIHYCVTNMPGAVPRTSTYALNNATLPYALKIAGSGLMDALKADISLVRGVNTLGGRLASRPVAEAHGL
EYIPLFRR

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=372, Percent_Identity=30.6451612903226, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI122939153, Length=372, Percent_Identity=30.6451612903226, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1787887, Length=335, Percent_Identity=29.2537313432836, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17550456, Length=359, Percent_Identity=27.2980501392758, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 43493; Mature: 43362

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLETCGSNIPCLVFLRAAAAFSPYERKGKRNERLKRGSMLIGVPKEIKDHEYRVALTP
CCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHHHHHCCEEEECCHHHCCCCEEEEECC
GSVGTLVRAGHKILVQSGAGEGSGFPDDEYRSSGAILFPEAADIWEGAGMVMKVKEPQPS
CCHHHHHHCCCEEEEECCCCCCCCCCCHHHCCCCCEEECCHHHHHCCCCEEEEECCCCCC
EYIYLRNDLILFTYLHLAAEPDLTRVLLDSGTAAIGYETVQLDNGTLPLLMPMSEVAGRM
CEEEEECCEEEEEHHHHHCCCCHHEEEECCCCEEEEEEEEEECCCCEEEEECHHHHHHHH
SAQIVAHYLEKENGGRGKLLGGVPGVRPADVVILGGGIVGSNAAKIALGMGAHVTIIDTS
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEECCCEECCCCEEEEEECCCEEEEEECC
SDRLRYLDEVLHGNLTTLGSNVQNIADSVNRADGLVGSVLIPGAKTPKLVSREMVAAMKP
CHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCC
GSVIVDVAIDQGGCVETARPTKHSDPVYIVDEVIHYCVTNMPGAVPRTSTYALNNATLPY
CCEEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCCE
ALKIAGSGLMDALKADISLVRGVNTLGGRLASRPVAEAHGLEYIPLFRR
EEEECCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHCCCEEECCCCC
>Mature Secondary Structure 
TRLETCGSNIPCLVFLRAAAAFSPYERKGKRNERLKRGSMLIGVPKEIKDHEYRVALTP
CCCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHHHHHCCEEEECCHHHCCCCEEEEECC
GSVGTLVRAGHKILVQSGAGEGSGFPDDEYRSSGAILFPEAADIWEGAGMVMKVKEPQPS
CCHHHHHHCCCEEEEECCCCCCCCCCCHHHCCCCCEEECCHHHHHCCCCEEEEECCCCCC
EYIYLRNDLILFTYLHLAAEPDLTRVLLDSGTAAIGYETVQLDNGTLPLLMPMSEVAGRM
CEEEEECCEEEEEHHHHHCCCCHHEEEECCCCEEEEEEEEEECCCCEEEEECHHHHHHHH
SAQIVAHYLEKENGGRGKLLGGVPGVRPADVVILGGGIVGSNAAKIALGMGAHVTIIDTS
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEECCCEECCCCEEEEEECCCEEEEEECC
SDRLRYLDEVLHGNLTTLGSNVQNIADSVNRADGLVGSVLIPGAKTPKLVSREMVAAMKP
CHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCC
GSVIVDVAIDQGGCVETARPTKHSDPVYIVDEVIHYCVTNMPGAVPRTSTYALNNATLPY
CCEEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCEEEECCCCCCE
ALKIAGSGLMDALKADISLVRGVNTLGGRLASRPVAEAHGLEYIPLFRR
EEEECCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]