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The map label for this gene is purC [H]

Identifier: 85858997

GI number: 85858997

Start: 1176009

End: 1176725

Strand: Direct

Name: purC [H]

Synonym: SYN_00107

Alternate gene names: 85858997

Gene position: 1176009-1176725 (Clockwise)

Preceding gene: 85858994

Following gene: 85858998

Centisome position: 36.99

GC content: 42.26

Gene sequence:

>717_bases
ATGAAAAACAAAAATACCCGACCAGCGCGGACTGGAAAAGCAGTTTTGCTGTATGAAGGAAAAACAAAAAATGTCTATAT
TCTTGAAAACGGCAATTATTTGCTGAAGTTCAAGGATGACGTTACAGGGCAAAACGGAGTTTTCGATCCGGGCGCGAATG
TTGTAGGGCTGAGCATCGAGGGGATCGGTAATCTGAATCTCAGGATGTCGATTTTTTTCTTTGAAAAGCTGAGGGTAGCA
GGTATTCAAACTCATTTTGTGAGTGCAAATCTTGAAAATTCAACAATGGAAGTGCTTCCCGCAAAGGTTTTCGGGAAAGG
TATCGAAGTCATCTGCCGCCGAAGGGCCGTTGGCAGTTTCTTACGCCGTTACGGGGACTTCATCGAAGAGGGAGCTCCCC
TTCCTTCTTACGTCGAGATGACCTTAAAGGACGACGATAGAAGCGATCCGCTTGTTACTAGGGATGGACTTATAGATCTT
GGTATTATGAGCGGTAAGCAGTATGATGATATCAAGAAAAAGACACAGGAAATTGCAAAAATTGTTGCCGACTGCCTTCT
TGAAAAGGGCATGGAGCTTTACGATATAAAATTGGAATTCGGTTGCAACGGGGATGAGATTATTCTCATTGATGAAATTT
CTTCAGGAAATATGCGAGTTTACAGGGATGGAAAATACATAGAGCCTATATCACTATCGGAACTGTTTCTTAAATAA

Upstream 100 bases:

>100_bases
TTTCGAATTTTGTGATGACGGCTATGACAGTGAAGATCATACCAGAGTGATGCGCTTGTAAGAAGAGTAAAATCTCGTAT
AGAAAACTCACATAAACGAC

Downstream 100 bases:

>100_bases
AATCGTTGCCGTGCTTTTCAGCGAACCGTTGCCGTTTTTCCCCTCCGTGTTAAAAAAGCTGCCGTTTTTTCACCTATATA
TCCGCAGGGAAGATATAGCA

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKNKNTRPARTGKAVLLYEGKTKNVYILENGNYLLKFKDDVTGQNGVFDPGANVVGLSIEGIGNLNLRMSIFFFEKLRVA
GIQTHFVSANLENSTMEVLPAKVFGKGIEVICRRRAVGSFLRRYGDFIEEGAPLPSYVEMTLKDDDRSDPLVTRDGLIDL
GIMSGKQYDDIKKKTQEIAKIVADCLLEKGMELYDIKLEFGCNGDEIILIDEISSGNMRVYRDGKYIEPISLSELFLK

Sequences:

>Translated_238_residues
MKNKNTRPARTGKAVLLYEGKTKNVYILENGNYLLKFKDDVTGQNGVFDPGANVVGLSIEGIGNLNLRMSIFFFEKLRVA
GIQTHFVSANLENSTMEVLPAKVFGKGIEVICRRRAVGSFLRRYGDFIEEGAPLPSYVEMTLKDDDRSDPLVTRDGLIDL
GIMSGKQYDDIKKKTQEIAKIVADCLLEKGMELYDIKLEFGCNGDEIILIDEISSGNMRVYRDGKYIEPISLSELFLK
>Mature_238_residues
MKNKNTRPARTGKAVLLYEGKTKNVYILENGNYLLKFKDDVTGQNGVFDPGANVVGLSIEGIGNLNLRMSIFFFEKLRVA
GIQTHFVSANLENSTMEVLPAKVFGKGIEVICRRRAVGSFLRRYGDFIEEGAPLPSYVEMTLKDDDRSDPLVTRDGLIDL
GIMSGKQYDDIKKKTQEIAKIVADCLLEKGMELYDIKLEFGCNGDEIILIDEISSGNMRVYRDGKYIEPISLSELFLK

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=222, Percent_Identity=32.4324324324324, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI5453539, Length=222, Percent_Identity=32.4324324324324, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI119220559, Length=229, Percent_Identity=31.0043668122271, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788820, Length=211, Percent_Identity=31.7535545023697, Blast_Score=81, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17531275, Length=221, Percent_Identity=31.6742081447964, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI18860083, Length=224, Percent_Identity=29.4642857142857, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 26677; Mature: 26677

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKNTRPARTGKAVLLYEGKTKNVYILENGNYLLKFKDDVTGQNGVFDPGANVVGLSIE
CCCCCCCCCCCCCEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEEE
GIGNLNLRMSIFFFEKLRVAGIQTHFVSANLENSTMEVLPAKVFGKGIEVICRRRAVGSF
CCCCCEEEEEEEEEEHHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
LRRYGDFIEEGAPLPSYVEMTLKDDDRSDPLVTRDGLIDLGIMSGKQYDDIKKKTQEIAK
HHHHHHHHHCCCCCCHHEEEEECCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHH
IVADCLLEKGMELYDIKLEFGCNGDEIILIDEISSGNMRVYRDGKYIEPISLSELFLK
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEEECCCCCEEEEECCCEECCCCHHHHHCC
>Mature Secondary Structure
MKNKNTRPARTGKAVLLYEGKTKNVYILENGNYLLKFKDDVTGQNGVFDPGANVVGLSIE
CCCCCCCCCCCCCEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEEE
GIGNLNLRMSIFFFEKLRVAGIQTHFVSANLENSTMEVLPAKVFGKGIEVICRRRAVGSF
CCCCCEEEEEEEEEEHHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
LRRYGDFIEEGAPLPSYVEMTLKDDDRSDPLVTRDGLIDLGIMSGKQYDDIKKKTQEIAK
HHHHHHHHHCCCCCCHHEEEEECCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHH
IVADCLLEKGMELYDIKLEFGCNGDEIILIDEISSGNMRVYRDGKYIEPISLSELFLK
HHHHHHHHCCCEEEEEEEEECCCCCEEEEEEECCCCCEEEEECCCEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]