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The map label for this gene is rlmI [H]

Identifier: 85858968

GI number: 85858968

Start: 1151315

End: 1152520

Strand: Direct

Name: rlmI [H]

Synonym: SYN_01029

Alternate gene names: 85858968

Gene position: 1151315-1152520 (Clockwise)

Preceding gene: 85858966

Following gene: 85858973

Centisome position: 36.21

GC content: 58.62

Gene sequence:

>1206_bases
GTGCTTTTCATGAAAATGAACTATCCCCGTCTGTATCTCAAGCCCGGCCGGGAAAAGGCTCTGTTGCATGGTCATCCCTG
GCTCTTTTCCGGGGCTGTGGCATCTGTTGAAGGAAAACGGGCTCCGGGAGATATCGTTGCCGTGACGGATGCCGGAGGCC
GGTCCCTGGGGTTGGGTTTCTTCAATTCCCGTTCGGATATCATCTTCCGATTTCTCACCGCCGATGTTGACACACGATGC
GACGCGCATTTCTGGCGGGAGCGGGTCCGGTTCGCCGAAGCATTACGACGGCGGATCCTTCCGCCGGATACGACGGCCTG
CCGGCTGATCAATGCCGAAGGTGATGGCCTGCCCGGCTTGATTGTGGACCGCTATGACGATGTCCTCGTCTGTTCCATCG
CTACGGCGGGCATGGAGAAAAACCGAACGGCTGTTCTGGATGCACTGACGGGTGTCTGTTCCCCCCGGGGAATCTATGAG
CGCAGTGAAGGACGGGCCAGGCGGCTTGAGGGGCTGGAAGAACGCAGCGGCTGGATTTACGGGAGTTGCGAGGAAGGGAA
GCGGATTCCCGTTCGGGAAAATGGTCTTTACTTCGAAGTCGATATTGTCGGGGGGCAGAAGACTGGGTTTTTTCTCGACC
AGCGCTCTAACCGGAAAGGGGTGGCCGGACTCAGCCGGGGGATGACCCTGCTGAACTGTTTCTCCTATAGCGGGGCTTTT
TCGGTTTACGCCGCCCGGGGCGGTGCTGAACGGGTCGTTTCCGTGGACGTGTCGGAAGGGGCGAACGACATGGCGCGGTT
CCATCTGAAAAAGAACGGATTTTCCACTGAACGGCACCCGGTGATCCGCGCCGACGTCTTTTCCTGGCTGCGGGAAACGG
AGGAGGTCTTTGACGGAATTATCCTCGATCCGCCGGCATTTGCCAAATCCCGCAAGGATATCGCCCGTTCCGCCAGAGGA
TACAAGGACATCAATCTCCAGGCTATGAAACATCTTCAAAACGGCGGGCTGCTGTGGACGTTTTCCTGCTCCAGCTTTAT
GGACGAGGCGCTTTTTCAGAAGGTGGTGCTGGGTGCTGCCTGCGACACGGGGAAAAGCGTCCAGCTGCTGAAGATCCTGG
GTCCGGGACCGGACCATCCCACCAGCCTGGCCCACATGGAAGGCCGGTATCTCAAGGGGCTGCTGCTGAGGGTTTCCGAT
CGTTGA

Upstream 100 bases:

>100_bases
TTGCGCCGGGAATAATCCGGCTTGTCTTTTTCCCGGCAAGTCGATATGTTCCGTCGAATTCTGAATGATAATCTGAATGA
TATAAAAAGATAAGGATTCT

Downstream 100 bases:

>100_bases
GGCGGAAGGCAGCCGAATTTCAATGATCGGAATCAAAAAAGAACATAAGAGACTAAAAAAACGATCCGGCAAGCAGTAAA
TTAAGCTCCAGGCTTTGCAG

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MLFMKMNYPRLYLKPGREKALLHGHPWLFSGAVASVEGKRAPGDIVAVTDAGGRSLGLGFFNSRSDIIFRFLTADVDTRC
DAHFWRERVRFAEALRRRILPPDTTACRLINAEGDGLPGLIVDRYDDVLVCSIATAGMEKNRTAVLDALTGVCSPRGIYE
RSEGRARRLEGLEERSGWIYGSCEEGKRIPVRENGLYFEVDIVGGQKTGFFLDQRSNRKGVAGLSRGMTLLNCFSYSGAF
SVYAARGGAERVVSVDVSEGANDMARFHLKKNGFSTERHPVIRADVFSWLRETEEVFDGIILDPPAFAKSRKDIARSARG
YKDINLQAMKHLQNGGLLWTFSCSSFMDEALFQKVVLGAACDTGKSVQLLKILGPGPDHPTSLAHMEGRYLKGLLLRVSD
R

Sequences:

>Translated_401_residues
MLFMKMNYPRLYLKPGREKALLHGHPWLFSGAVASVEGKRAPGDIVAVTDAGGRSLGLGFFNSRSDIIFRFLTADVDTRC
DAHFWRERVRFAEALRRRILPPDTTACRLINAEGDGLPGLIVDRYDDVLVCSIATAGMEKNRTAVLDALTGVCSPRGIYE
RSEGRARRLEGLEERSGWIYGSCEEGKRIPVRENGLYFEVDIVGGQKTGFFLDQRSNRKGVAGLSRGMTLLNCFSYSGAF
SVYAARGGAERVVSVDVSEGANDMARFHLKKNGFSTERHPVIRADVFSWLRETEEVFDGIILDPPAFAKSRKDIARSARG
YKDINLQAMKHLQNGGLLWTFSCSSFMDEALFQKVVLGAACDTGKSVQLLKILGPGPDHPTSLAHMEGRYLKGLLLRVSD
R
>Mature_401_residues
MLFMKMNYPRLYLKPGREKALLHGHPWLFSGAVASVEGKRAPGDIVAVTDAGGRSLGLGFFNSRSDIIFRFLTADVDTRC
DAHFWRERVRFAEALRRRILPPDTTACRLINAEGDGLPGLIVDRYDDVLVCSIATAGMEKNRTAVLDALTGVCSPRGIYE
RSEGRARRLEGLEERSGWIYGSCEEGKRIPVRENGLYFEVDIVGGQKTGFFLDQRSNRKGVAGLSRGMTLLNCFSYSGAF
SVYAARGGAERVVSVDVSEGANDMARFHLKKNGFSTERHPVIRADVFSWLRETEEVFDGIILDPPAFAKSRKDIARSARG
YKDINLQAMKHLQNGGLLWTFSCSSFMDEALFQKVVLGAACDTGKSVQLLKILGPGPDHPTSLAHMEGRYLKGLLLRVSD
R

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=393, Percent_Identity=40.2035623409669, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1787181, Length=220, Percent_Identity=32.2727272727273, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44421; Mature: 44421

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFMKMNYPRLYLKPGREKALLHGHPWLFSGAVASVEGKRAPGDIVAVTDAGGRSLGLGF
CEEEEECCCEEEECCCCCCEEEECCCCEECCCEEECCCCCCCCCEEEEECCCCCEEEEEE
FNSRSDIIFRFLTADVDTRCDAHFWRERVRFAEALRRRILPPDTTACRLINAEGDGLPGL
CCCCHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCE
IVDRYDDVLVCSIATAGMEKNRTAVLDALTGVCSPRGIYERSEGRARRLEGLEERSGWIY
EEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHCCEEE
GSCEEGKRIPVRENGLYFEVDIVGGQKTGFFLDQRSNRKGVAGLSRGMTLLNCFSYSGAF
EECCCCCCCCEECCCEEEEEEEECCCCCCEEEECCCCCCCCHHHHHCHHHHHHHCCCCCE
SVYAARGGAERVVSVDVSEGANDMARFHLKKNGFSTERHPVIRADVFSWLRETEEVFDGI
EEEEECCCCCEEEEEECCCCCCHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCE
ILDPPAFAKSRKDIARSARGYKDINLQAMKHLQNGGLLWTFSCSSFMDEALFQKVVLGAA
EECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHC
CDTGKSVQLLKILGPGPDHPTSLAHMEGRYLKGLLLRVSDR
CCCCCCEEEEEEECCCCCCCCCHHHHCCHHHEEEEEEECCC
>Mature Secondary Structure
MLFMKMNYPRLYLKPGREKALLHGHPWLFSGAVASVEGKRAPGDIVAVTDAGGRSLGLGF
CEEEEECCCEEEECCCCCCEEEECCCCEECCCEEECCCCCCCCCEEEEECCCCCEEEEEE
FNSRSDIIFRFLTADVDTRCDAHFWRERVRFAEALRRRILPPDTTACRLINAEGDGLPGL
CCCCHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCE
IVDRYDDVLVCSIATAGMEKNRTAVLDALTGVCSPRGIYERSEGRARRLEGLEERSGWIY
EEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHCCEEE
GSCEEGKRIPVRENGLYFEVDIVGGQKTGFFLDQRSNRKGVAGLSRGMTLLNCFSYSGAF
EECCCCCCCCEECCCEEEEEEEECCCCCCEEEECCCCCCCCHHHHHCHHHHHHHCCCCCE
SVYAARGGAERVVSVDVSEGANDMARFHLKKNGFSTERHPVIRADVFSWLRETEEVFDGI
EEEEECCCCCEEEEEECCCCCCHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCE
ILDPPAFAKSRKDIARSARGYKDINLQAMKHLQNGGLLWTFSCSSFMDEALFQKVVLGAA
EECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHC
CDTGKSVQLLKILGPGPDHPTSLAHMEGRYLKGLLLRVSDR
CCCCCCEEEEEEECCCCCCCCCHHHHCCHHHEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA