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The map label for this gene is ntrX [H]

Identifier: 85858790

GI number: 85858790

Start: 981743

End: 983116

Strand: Direct

Name: ntrX [H]

Synonym: SYN_02713

Alternate gene names: 85858790

Gene position: 981743-983116 (Clockwise)

Preceding gene: 85858789

Following gene: 85858802

Centisome position: 30.88

GC content: 48.76

Gene sequence:

>1374_bases
ATGCAAAAATCAATACTGATTGTCGATGATGAAGAGAGTATCTGCAAGTCCCTGGGAGATATTCTTGTTGATGAAGGGTA
TGAGGTCCAGACTGTGCAGAGCGGCGAAGAGGCCATAAGACTCCTTGACGAGGATCTTCCCGGCATTGTGCTTCTGGATA
TTTGGCTGCCCGGGATAGACGGGATGGAAGTCCTGAAAACGATTAAAAGCCTGCATCCCAAACTGCCGGTGGTCATGATG
TCCGGCCACGGAACGATAGAAACCGCTGTGAAGGCGACTAAACTGGGCGCCTTCGACTTCATTGAGAAGCCTCTTTCTCT
GGAGAAGATCATCCTGACGCTGAAACATGCTCTGGATTTGTCCCGCCTTGAAGAAGAAAATGTTTTACTGAAACAGAAAA
TTAACCGGGATTATGAGCTGACCGGTAAAAGTCCCCCGATTATCGAGCTGATGGAAATGATCAGCATTGTGGCTCCCACT
AATGCCTGGATTCTCATCATGGGGGAAAATGGAACGGGTAAGGAACTGGTAGCCCGTGCGATTCACCGTCAGAGCAAGCG
GGCGGACAAGCCAATGGTTGAAGTGAATTGCGCGGCCATTCCCGAAGAACTTATCGAGAGCGAACTTTTTGGTCATGAGA
AGGGCGCTTTTACCGGGGCCACGGAGAAGAAAAGGGGAAAATTCGATATCGCCCATGAAGGAACCATATTCCTTGACGAA
GTGGCGGACATGAGTTTGAAGGCCCAAGCCAAGATTCTGAGAATCCTGCAGGAAAAAAAGTTTGAACGGGTTGGAGGGAA
CAAGGTGATCCAGCTCGACGTACGCGTCCTTGCCGCTACGAACAAAGATCTTGAAAAAGAAATGGAAGAAGGCCGATTCA
GGCAGGACCTGTTTTATCGGCTTAATGTCATTCCGCTGAAAGTTCCTCCCCTGAGAAGCAGAAAAGAAGATATCCCTCTG
CTGGCGGAACGGTTTCTGAGCGATTTTGCCCTGAAAGAGGGAGAGCCCGGAAAAACAATTCAGGATGATGCCTTGGCTCT
TTTAATGGAGCATTCATGGCCGGGTAATGTGCGGGAACTCAAGAATTTCATTGAACGACTGGCGATCATGACGACCTCGA
ATGTGATCACCGTTCATGACATTCCACCTCTTCCAAGGGATGGCCATAAAGTCGAGCCATTCAGAGAACCCGGATTTGAG
CTCTTGGCCGGCTCGTTCCGGGAGGCCAAGATGGATTTTGAAAAACAGTATATTTCCAGAAAACTCCGTGAATTTGACGG
AAACATCTCAAAAACAGCCGAGTCCATTGGCCTGGAGCGAAGCAATCTGCACAAGAAAATCAAGGCCTATGGTCTGGAGA
GCAAAAACGATTAA

Upstream 100 bases:

>100_bases
GAAATATCATTTTGGCTGGTGTTAATGACACCCATAATCTGAGAGAATGTGTTAGAATATAAAAAATGTTCTACTCATCA
AATATTTAAAAAGTTGGTTT

Downstream 100 bases:

>100_bases
GAGTGCCGGCTTGGATTTAAAGGAATTTCAGAAAGCATGAAAGGAAAATCCACGGAGGGAGAGGAAAATAGTTCAGGAAC
GACGGCAATTCAACCTGCAG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MQKSILIVDDEESICKSLGDILVDEGYEVQTVQSGEEAIRLLDEDLPGIVLLDIWLPGIDGMEVLKTIKSLHPKLPVVMM
SGHGTIETAVKATKLGAFDFIEKPLSLEKIILTLKHALDLSRLEEENVLLKQKINRDYELTGKSPPIIELMEMISIVAPT
NAWILIMGENGTGKELVARAIHRQSKRADKPMVEVNCAAIPEELIESELFGHEKGAFTGATEKKRGKFDIAHEGTIFLDE
VADMSLKAQAKILRILQEKKFERVGGNKVIQLDVRVLAATNKDLEKEMEEGRFRQDLFYRLNVIPLKVPPLRSRKEDIPL
LAERFLSDFALKEGEPGKTIQDDALALLMEHSWPGNVRELKNFIERLAIMTTSNVITVHDIPPLPRDGHKVEPFREPGFE
LLAGSFREAKMDFEKQYISRKLREFDGNISKTAESIGLERSNLHKKIKAYGLESKND

Sequences:

>Translated_457_residues
MQKSILIVDDEESICKSLGDILVDEGYEVQTVQSGEEAIRLLDEDLPGIVLLDIWLPGIDGMEVLKTIKSLHPKLPVVMM
SGHGTIETAVKATKLGAFDFIEKPLSLEKIILTLKHALDLSRLEEENVLLKQKINRDYELTGKSPPIIELMEMISIVAPT
NAWILIMGENGTGKELVARAIHRQSKRADKPMVEVNCAAIPEELIESELFGHEKGAFTGATEKKRGKFDIAHEGTIFLDE
VADMSLKAQAKILRILQEKKFERVGGNKVIQLDVRVLAATNKDLEKEMEEGRFRQDLFYRLNVIPLKVPPLRSRKEDIPL
LAERFLSDFALKEGEPGKTIQDDALALLMEHSWPGNVRELKNFIERLAIMTTSNVITVHDIPPLPRDGHKVEPFREPGFE
LLAGSFREAKMDFEKQYISRKLREFDGNISKTAESIGLERSNLHKKIKAYGLESKND
>Mature_457_residues
MQKSILIVDDEESICKSLGDILVDEGYEVQTVQSGEEAIRLLDEDLPGIVLLDIWLPGIDGMEVLKTIKSLHPKLPVVMM
SGHGTIETAVKATKLGAFDFIEKPLSLEKIILTLKHALDLSRLEEENVLLKQKINRDYELTGKSPPIIELMEMISIVAPT
NAWILIMGENGTGKELVARAIHRQSKRADKPMVEVNCAAIPEELIESELFGHEKGAFTGATEKKRGKFDIAHEGTIFLDE
VADMSLKAQAKILRILQEKKFERVGGNKVIQLDVRVLAATNKDLEKEMEEGRFRQDLFYRLNVIPLKVPPLRSRKEDIPL
LAERFLSDFALKEGEPGKTIQDDALALLMEHSWPGNVRELKNFIERLAIMTTSNVITVHDIPPLPRDGHKVEPFREPGFE
LLAGSFREAKMDFEKQYISRKLREFDGNISKTAESIGLERSNLHKKIKAYGLESKND

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=456, Percent_Identity=42.5438596491228, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1790437, Length=454, Percent_Identity=41.6299559471366, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI1790299, Length=473, Percent_Identity=38.0549682875264, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1788905, Length=452, Percent_Identity=38.716814159292, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI87082117, Length=346, Percent_Identity=43.3526011560694, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1789087, Length=339, Percent_Identity=43.952802359882, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI87082152, Length=329, Percent_Identity=42.5531914893617, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789233, Length=323, Percent_Identity=42.1052631578947, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786524, Length=358, Percent_Identity=37.7094972067039, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1787583, Length=351, Percent_Identity=35.8974358974359, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI87081872, Length=327, Percent_Identity=38.2262996941896, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI87081858, Length=299, Percent_Identity=30.7692307692308, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789828, Length=248, Percent_Identity=33.8709677419355, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786599, Length=140, Percent_Identity=30.7142857142857, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789809, Length=114, Percent_Identity=28.0701754385965, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790860, Length=119, Percent_Identity=27.7310924369748, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87082012, Length=114, Percent_Identity=26.3157894736842, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786784, Length=114, Percent_Identity=28.0701754385965, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51502; Mature: 51502

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSILIVDDEESICKSLGDILVDEGYEVQTVQSGEEAIRLLDEDLPGIVLLDIWLPGID
CCCCEEEEECHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCCC
GMEVLKTIKSLHPKLPVVMMSGHGTIETAVKATKLGAFDFIEKPLSLEKIILTLKHALDL
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
SRLEEENVLLKQKINRDYELTGKSPPIIELMEMISIVAPTNAWILIMGENGTGKELVARA
HHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
IHRQSKRADKPMVEVNCAAIPEELIESELFGHEKGAFTGATEKKRGKFDIAHEGTIFLDE
HHHHHHHCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCEEEHH
VADMSLKAQAKILRILQEKKFERVGGNKVIQLDVRVLAATNKDLEKEMEEGRFRQDLFYR
HHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHHHHCHHHHHHHHE
LNVIPLKVPPLRSRKEDIPLLAERFLSDFALKEGEPGKTIQDDALALLMEHSWPGNVREL
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
KNFIERLAIMTTSNVITVHDIPPLPRDGHKVEPFREPGFELLAGSFREAKMDFEKQYISR
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHH
KLREFDGNISKTAESIGLERSNLHKKIKAYGLESKND
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQKSILIVDDEESICKSLGDILVDEGYEVQTVQSGEEAIRLLDEDLPGIVLLDIWLPGID
CCCCEEEEECHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCCC
GMEVLKTIKSLHPKLPVVMMSGHGTIETAVKATKLGAFDFIEKPLSLEKIILTLKHALDL
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
SRLEEENVLLKQKINRDYELTGKSPPIIELMEMISIVAPTNAWILIMGENGTGKELVARA
HHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
IHRQSKRADKPMVEVNCAAIPEELIESELFGHEKGAFTGATEKKRGKFDIAHEGTIFLDE
HHHHHHHCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCEEEHH
VADMSLKAQAKILRILQEKKFERVGGNKVIQLDVRVLAATNKDLEKEMEEGRFRQDLFYR
HHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHHHHCHHHHHHHHE
LNVIPLKVPPLRSRKEDIPLLAERFLSDFALKEGEPGKTIQDDALALLMEHSWPGNVREL
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
KNFIERLAIMTTSNVITVHDIPPLPRDGHKVEPFREPGFELLAGSFREAKMDFEKQYISR
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHH
KLREFDGNISKTAESIGLERSNLHKKIKAYGLESKND
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]