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The map label for this gene is yjeA [H]

Identifier: 85858716

GI number: 85858716

Start: 895474

End: 896472

Strand: Direct

Name: yjeA [H]

Synonym: SYN_00184

Alternate gene names: 85858716

Gene position: 895474-896472 (Clockwise)

Preceding gene: 85858715

Following gene: 85858717

Centisome position: 28.17

GC content: 56.86

Gene sequence:

>999_bases
ATGGCTGACGCTTTCTTCTGCAAACCCTCCCTGACGGATCTATCCCCAAAAAAACTCACCGGGGAAGATTCCCGCTATGA
TGAAGGCTGGAGGCTGGCCGGAAAACGGGAGACCCTGAGGCTCCGGGCGGAAATTCTTCACAGCATACGCCGGTTTTTCC
GGGAACGGGACTATCTGGAGGTGGATACGCCCATTCTGATACCAGCCCCGGCTCCGGAATCGCACATCGATGCCGTCGCC
TGTGGCTTCCAGTACCTGCAGACTTCGCCGGAACTCTGCATGAAAAGGCTGCTTGCCGCCGGATATCCGAGACTTTTTCA
GATCTGCAGCTGTTTCCGCGAGGCGGAACGAGGGACACGCCATCTCCCTGAATTTACTCTCCTTGAATGGTATGCTCAAG
GAAAGGATTACATGTTTCTCATGGAGGAGTGCGAAGCCCTGCTGCGCTTTCTTGCCGTTACTCTGTACGGGAAAGAGGAG
ATCACCTTCCAGGGGAGCCGCATCGACCTGTCAGCTCCGTGGGAGCGGCTGTCCGTGAACGAAGCCTTTCTGCGTTATGG
AGGGATATCGGCCCGGCAGACCCTGGCGGAGGACATTTTTGACGAAGTTCTCGTCTCCCGGATTGAATCCAGACTGGGGA
TAGAACGACCGACCTTTCTGTACGATTATCCTGTTGAACTGGCCGCCCTGGCACGGGCGAAACCGAATGATCCCTCTCTG
GCCGAGCGGTTTGAGCTTTATGTTGCCGGGATCGAACTGGCCAATGCCTTTTCCGAACTGATCGATCCCGTTGAACAGAG
CCGCCGCTTCGAAGAAGCACAGCAGGAACGCCGGGCTCTTGGCAAAAGCCCCTATCCCTGGCCGGAGCGTTTTCTGCGCG
CACTATCCGCAATGCCCCCTTCGACGGGCATTGCCCTGGGGATCGATCGGCTGGTGATGCTGATGACGGATCGTGCGTTC
ATCGATGATGTGGTGGCCTTTCCTCCGGAACGATTATGA

Upstream 100 bases:

>100_bases
TCAGATCATGGTGCCTCCCTTTATCGAAGAGGGGGAGAAAATCCGCGTGGATACCCGGACAGGCGAATATCTGACCCGGG
TCAAGGGCTGATAACCGGAC

Downstream 100 bases:

>100_bases
AATCGGGGCAGGATAAAGAGTCGGTAAGGATGCCGCTGGAAAATGGCATATTGACAAGGCCTCCTTTTTTCATTAGACAA
TAGCCTCCATGCCAATATTT

Product: truncated lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MADAFFCKPSLTDLSPKKLTGEDSRYDEGWRLAGKRETLRLRAEILHSIRRFFRERDYLEVDTPILIPAPAPESHIDAVA
CGFQYLQTSPELCMKRLLAAGYPRLFQICSCFREAERGTRHLPEFTLLEWYAQGKDYMFLMEECEALLRFLAVTLYGKEE
ITFQGSRIDLSAPWERLSVNEAFLRYGGISARQTLAEDIFDEVLVSRIESRLGIERPTFLYDYPVELAALARAKPNDPSL
AERFELYVAGIELANAFSELIDPVEQSRRFEEAQQERRALGKSPYPWPERFLRALSAMPPSTGIALGIDRLVMLMTDRAF
IDDVVAFPPERL

Sequences:

>Translated_332_residues
MADAFFCKPSLTDLSPKKLTGEDSRYDEGWRLAGKRETLRLRAEILHSIRRFFRERDYLEVDTPILIPAPAPESHIDAVA
CGFQYLQTSPELCMKRLLAAGYPRLFQICSCFREAERGTRHLPEFTLLEWYAQGKDYMFLMEECEALLRFLAVTLYGKEE
ITFQGSRIDLSAPWERLSVNEAFLRYGGISARQTLAEDIFDEVLVSRIESRLGIERPTFLYDYPVELAALARAKPNDPSL
AERFELYVAGIELANAFSELIDPVEQSRRFEEAQQERRALGKSPYPWPERFLRALSAMPPSTGIALGIDRLVMLMTDRAF
IDDVVAFPPERL
>Mature_331_residues
ADAFFCKPSLTDLSPKKLTGEDSRYDEGWRLAGKRETLRLRAEILHSIRRFFRERDYLEVDTPILIPAPAPESHIDAVAC
GFQYLQTSPELCMKRLLAAGYPRLFQICSCFREAERGTRHLPEFTLLEWYAQGKDYMFLMEECEALLRFLAVTLYGKEEI
TFQGSRIDLSAPWERLSVNEAFLRYGGISARQTLAEDIFDEVLVSRIESRLGIERPTFLYDYPVELAALARAKPNDPSLA
ERFELYVAGIELANAFSELIDPVEQSRRFEEAQQERRALGKSPYPWPERFLRALSAMPPSTGIALGIDRLVMLMTDRAFI
DDVVAFPPERL

Specific function: Unknown

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=336, Percent_Identity=29.4642857142857, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI194272210, Length=336, Percent_Identity=29.4642857142857, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI1789256, Length=323, Percent_Identity=34.6749226006192, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790571, Length=329, Percent_Identity=34.0425531914894, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082379, Length=320, Percent_Identity=36.875, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71994340, Length=337, Percent_Identity=28.1899109792285, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17535927, Length=337, Percent_Identity=28.1899109792285, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17535925, Length=337, Percent_Identity=28.1899109792285, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320242, Length=327, Percent_Identity=29.0519877675841, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324256, Length=361, Percent_Identity=27.1468144044321, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24640851, Length=336, Percent_Identity=27.6785714285714, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24640849, Length=336, Percent_Identity=27.6785714285714, Blast_Score=127, Evalue=8e-30,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 38108; Mature: 37977

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAFFCKPSLTDLSPKKLTGEDSRYDEGWRLAGKRETLRLRAEILHSIRRFFRERDYLE
CCCCEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
VDTPILIPAPAPESHIDAVACGFQYLQTSPELCMKRLLAAGYPRLFQICSCFREAERGTR
ECCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HLPEFTLLEWYAQGKDYMFLMEECEALLRFLAVTLYGKEEITFQGSRIDLSAPWERLSVN
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCHHHHHHH
EAFLRYGGISARQTLAEDIFDEVLVSRIESRLGIERPTFLYDYPVELAALARAKPNDPSL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCCCHH
AERFELYVAGIELANAFSELIDPVEQSRRFEEAQQERRALGKSPYPWPERFLRALSAMPP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
STGIALGIDRLVMLMTDRAFIDDVVAFPPERL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADAFFCKPSLTDLSPKKLTGEDSRYDEGWRLAGKRETLRLRAEILHSIRRFFRERDYLE
CCCEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
VDTPILIPAPAPESHIDAVACGFQYLQTSPELCMKRLLAAGYPRLFQICSCFREAERGTR
ECCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HLPEFTLLEWYAQGKDYMFLMEECEALLRFLAVTLYGKEEITFQGSRIDLSAPWERLSVN
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCHHHHHHH
EAFLRYGGISARQTLAEDIFDEVLVSRIESRLGIERPTFLYDYPVELAALARAKPNDPSL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCCCHH
AERFELYVAGIELANAFSELIDPVEQSRRFEEAQQERRALGKSPYPWPERFLRALSAMPP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
STGIALGIDRLVMLMTDRAFIDDVVAFPPERL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA