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The map label for this gene is kinE [H]

Identifier: 85858685

GI number: 85858685

Start: 861038

End: 862996

Strand: Direct

Name: kinE [H]

Synonym: SYN_00882

Alternate gene names: 85858685

Gene position: 861038-862996 (Clockwise)

Preceding gene: 85858684

Following gene: 85858686

Centisome position: 27.08

GC content: 50.08

Gene sequence:

>1959_bases
ATGATGAAGATGAAAATCATGACGAAAGCACAGCGTATGGCCGATCTGGAATATATGAAAAAAAAGGTGCCCGCTCTTGA
GAAAAATCCAGGCGAAGAAGACTTTGCCGGGTCATTTTCAGGCGAAGAGAAATATCGCAGGCTTCTTGAAAACCTGAGTG
ATATGGTTTTCCTTCTGAGGATTCCTGAAGGCGATTATGAATATATCAGCCCTTCCGTTGTGGAAGTCTTTGGTTACAGC
GCGTCGGATTTTTATCGGAACCCGTTTTTCATGAAGGACATTATCCATCCCGATTATTTCGACTACTGCAATGAAAAATG
GGAGGAACTGCTGGAGGGAATCGCGGGGCGCAGCTATGAATATAAAATAATTGATCCCAAAGGGAAAGAACGATGGATTC
ACCAGTCCAATAAAGGCATCTTCGACCCGGAAGACAGGCTGATTGCCCTGGTCGGGCTGTGCCGTGACATCACGGGGTCA
AGACACACGGAAACGACCCTGAAAGAGAGTGAACGCAAGTATCGGCGTGTCTTTGAAGTGACAGGCGACGCCGTTTTTCT
GGTAGAGAAAAAAACGGGCTCCATCCTTGATGCCAATGCCGCGGCAACAGGACTTTATGGATACACTCTGGTGGAAATGA
CGAAACGCCGATTGATCGATCTTTCCACCGAACCGGACAGAACAGTCCTGATGCTGAGGGCGGGGGATACTCAAATCCAG
CAGCAGGACCACAAAAAGAAAGATGGCACGATCTTTCCCGTTGAAGCGAAGGTCAGTTACTTTACGCAGAAACACAGGAA
AATGGCAGTCGTGACTGTCCGCGATATTTCGGAACGCAAACGGTCTGAAAAGGCGATTCGTGAGAGTGAGGCCCGCTATC
GCAGTTTCTTTGAAGAAAATGTTGCCATTCTGCTGATGATCAATCCCGATACCGGCCGGATTGTGGATGCCAATCCGGCG
GCTTGTCATTTCTATGGCTACAGCAGGGAAATCCTTAAATTGAAGATGATCGACGACATCAACATCCTTTCCCGGGAACA
GATCCTGCGTGACATGGAGCGGGCGAGAAACCGGGAATGTCAGCATTTCTATTTCCTGAACCGTCTGGCCAGCGGCGAAG
TCAGAGATGTGGAGGCCTATATCGGTCCTACCGAGATGGCCGGCCAGCACCTTCTCTATTTTCTCGTGCACGATATTACG
GACCGCAAGCGAATGGAAGAAGAACTGGTCAAGGCTCAGAAGCTTGAGTCCGTAGGTATCCTGGCAGGGGGTATTGCTCA
TGACTTCAATAATATCCTTGCTGCGATCCTTGGGAACGTTTCTCTGGCCAAGCTTTATCTCTCTCCTGATGACCCGGCTT
ACGACAAAATGACTCAAGCGGAAAAAAGCTGTCTGCAGGCCAGGGAACTGACCGGCCGGTTGATCACTTTTTCGAAGGGA
GGAGAACCGCTGCGGAAAAAAGTGGCCGTTGCCCCCTTCCTCAAGGATGCCGTGTCTTTTTTCCTGAGTGGTTCAAATGT
GCGCTGCGAATTTGACTTTCCCGATTTCCTGTGGCCTCTGGAAATTGATGTGGGGCAAATGCAGCAGGTGGTGCGTCACC
TGATTGTGAATGCCCAGGAGGCCATGCCGGATGGAGGAACCATCCGGATCAGTGCGGAAAATGTGAGTTTGAAGGCCGAC
GAAGTGCCGTCCCTGAAGGAAGGTCCCTATGTCCGGATATGCATCAAAGACCAGGGAGTGGGCATTCCCCGTGAGTATCA
GACCAGAGTCTTCGATCCTTATTTCACCACAAAGCCTATGGGAGGAACCAAAGGGGCGGGGCTCGGACTGGCGATCTGCT
ATTCCACCATTAAGAAGCATGAAGGTTTTCTGTCCTTAGTATCCCAACCGGGTGTGGAAACAACCATTAACATTTACCTG
CCCGCGTCATCGGATGATATGAAATCCGTCAAACCATAG

Upstream 100 bases:

>100_bases
GGGATGACCACGCAGGAGAGTTTTCCAAATTGAGTCGACGGGGCAAGAGGATCATGGAGATCAGGTTGCGTGACAATGCG
AATTTTCAGGAAGCCCTCGG

Downstream 100 bases:

>100_bases
CCGGAGAGAATTTTGCGGATCCTGATGTTCGGCTGGGAGTTTCCGCCTTATATGAGCGGCGGCCTGGGCACTGCCTGCTC
TGGTTTGACCGGGGCGCTGA

Product: signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MMKMKIMTKAQRMADLEYMKKKVPALEKNPGEEDFAGSFSGEEKYRRLLENLSDMVFLLRIPEGDYEYISPSVVEVFGYS
ASDFYRNPFFMKDIIHPDYFDYCNEKWEELLEGIAGRSYEYKIIDPKGKERWIHQSNKGIFDPEDRLIALVGLCRDITGS
RHTETTLKESERKYRRVFEVTGDAVFLVEKKTGSILDANAAATGLYGYTLVEMTKRRLIDLSTEPDRTVLMLRAGDTQIQ
QQDHKKKDGTIFPVEAKVSYFTQKHRKMAVVTVRDISERKRSEKAIRESEARYRSFFEENVAILLMINPDTGRIVDANPA
ACHFYGYSREILKLKMIDDINILSREQILRDMERARNRECQHFYFLNRLASGEVRDVEAYIGPTEMAGQHLLYFLVHDIT
DRKRMEEELVKAQKLESVGILAGGIAHDFNNILAAILGNVSLAKLYLSPDDPAYDKMTQAEKSCLQARELTGRLITFSKG
GEPLRKKVAVAPFLKDAVSFFLSGSNVRCEFDFPDFLWPLEIDVGQMQQVVRHLIVNAQEAMPDGGTIRISAENVSLKAD
EVPSLKEGPYVRICIKDQGVGIPREYQTRVFDPYFTTKPMGGTKGAGLGLAICYSTIKKHEGFLSLVSQPGVETTINIYL
PASSDDMKSVKP

Sequences:

>Translated_652_residues
MMKMKIMTKAQRMADLEYMKKKVPALEKNPGEEDFAGSFSGEEKYRRLLENLSDMVFLLRIPEGDYEYISPSVVEVFGYS
ASDFYRNPFFMKDIIHPDYFDYCNEKWEELLEGIAGRSYEYKIIDPKGKERWIHQSNKGIFDPEDRLIALVGLCRDITGS
RHTETTLKESERKYRRVFEVTGDAVFLVEKKTGSILDANAAATGLYGYTLVEMTKRRLIDLSTEPDRTVLMLRAGDTQIQ
QQDHKKKDGTIFPVEAKVSYFTQKHRKMAVVTVRDISERKRSEKAIRESEARYRSFFEENVAILLMINPDTGRIVDANPA
ACHFYGYSREILKLKMIDDINILSREQILRDMERARNRECQHFYFLNRLASGEVRDVEAYIGPTEMAGQHLLYFLVHDIT
DRKRMEEELVKAQKLESVGILAGGIAHDFNNILAAILGNVSLAKLYLSPDDPAYDKMTQAEKSCLQARELTGRLITFSKG
GEPLRKKVAVAPFLKDAVSFFLSGSNVRCEFDFPDFLWPLEIDVGQMQQVVRHLIVNAQEAMPDGGTIRISAENVSLKAD
EVPSLKEGPYVRICIKDQGVGIPREYQTRVFDPYFTTKPMGGTKGAGLGLAICYSTIKKHEGFLSLVSQPGVETTINIYL
PASSDDMKSVKP
>Mature_652_residues
MMKMKIMTKAQRMADLEYMKKKVPALEKNPGEEDFAGSFSGEEKYRRLLENLSDMVFLLRIPEGDYEYISPSVVEVFGYS
ASDFYRNPFFMKDIIHPDYFDYCNEKWEELLEGIAGRSYEYKIIDPKGKERWIHQSNKGIFDPEDRLIALVGLCRDITGS
RHTETTLKESERKYRRVFEVTGDAVFLVEKKTGSILDANAAATGLYGYTLVEMTKRRLIDLSTEPDRTVLMLRAGDTQIQ
QQDHKKKDGTIFPVEAKVSYFTQKHRKMAVVTVRDISERKRSEKAIRESEARYRSFFEENVAILLMINPDTGRIVDANPA
ACHFYGYSREILKLKMIDDINILSREQILRDMERARNRECQHFYFLNRLASGEVRDVEAYIGPTEMAGQHLLYFLVHDIT
DRKRMEEELVKAQKLESVGILAGGIAHDFNNILAAILGNVSLAKLYLSPDDPAYDKMTQAEKSCLQARELTGRLITFSKG
GEPLRKKVAVAPFLKDAVSFFLSGSNVRCEFDFPDFLWPLEIDVGQMQQVVRHLIVNAQEAMPDGGTIRISAENVSLKAD
EVPSLKEGPYVRICIKDQGVGIPREYQTRVFDPYFTTKPMGGTKGAGLGLAICYSTIKKHEGFLSLVSQPGVETTINIYL
PASSDDMKSVKP

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=376, Percent_Identity=26.3297872340426, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081881, Length=135, Percent_Identity=40, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790436, Length=252, Percent_Identity=26.5873015873016, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI145693157, Length=338, Percent_Identity=26.0355029585799, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790300, Length=246, Percent_Identity=30.0813008130081, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 74208; Mature: 74208

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKMKIMTKAQRMADLEYMKKKVPALEKNPGEEDFAGSFSGEEKYRRLLENLSDMVFLLR
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEE
IPEGDYEYISPSVVEVFGYSASDFYRNPFFMKDIIHPDYFDYCNEKWEELLEGIAGRSYE
CCCCCCCCCCCHHHHHHCCCHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEE
YKIIDPKGKERWIHQSNKGIFDPEDRLIALVGLCRDITGSRHTETTLKESERKYRRVFEV
EEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
TGDAVFLVEKKTGSILDANAAATGLYGYTLVEMTKRRLIDLSTEPDRTVLMLRAGDTQIQ
CCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCHHH
QQDHKKKDGTIFPVEAKVSYFTQKHRKMAVVTVRDISERKRSEKAIRESEARYRSFFEEN
HHHCCCCCCCEEEEHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
VAILLMINPDTGRIVDANPAACHFYGYSREILKLKMIDDINILSREQILRDMERARNREC
CEEEEEECCCCCCEEECCCCEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
QHFYFLNRLASGEVRDVEAYIGPTEMAGQHLLYFLVHDITDRKRMEEELVKAQKLESVGI
HHHHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGGIAHDFNNILAAILGNVSLAKLYLSPDDPAYDKMTQAEKSCLQARELTGRLITFSKG
EECHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
GEPLRKKVAVAPFLKDAVSFFLSGSNVRCEFDFPDFLWPLEIDVGQMQQVVRHLIVNAQE
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHH
AMPDGGTIRISAENVSLKADEVPSLKEGPYVRICIKDQGVGIPREYQTRVFDPYFTTKPM
HCCCCCEEEEEECCEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHEEECCCCCCCCC
GGTKGAGLGLAICYSTIKKHEGFLSLVSQPGVETTINIYLPASSDDMKSVKP
CCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MMKMKIMTKAQRMADLEYMKKKVPALEKNPGEEDFAGSFSGEEKYRRLLENLSDMVFLLR
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEE
IPEGDYEYISPSVVEVFGYSASDFYRNPFFMKDIIHPDYFDYCNEKWEELLEGIAGRSYE
CCCCCCCCCCCHHHHHHCCCHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEE
YKIIDPKGKERWIHQSNKGIFDPEDRLIALVGLCRDITGSRHTETTLKESERKYRRVFEV
EEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
TGDAVFLVEKKTGSILDANAAATGLYGYTLVEMTKRRLIDLSTEPDRTVLMLRAGDTQIQ
CCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCHHH
QQDHKKKDGTIFPVEAKVSYFTQKHRKMAVVTVRDISERKRSEKAIRESEARYRSFFEEN
HHHCCCCCCCEEEEHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
VAILLMINPDTGRIVDANPAACHFYGYSREILKLKMIDDINILSREQILRDMERARNREC
CEEEEEECCCCCCEEECCCCEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
QHFYFLNRLASGEVRDVEAYIGPTEMAGQHLLYFLVHDITDRKRMEEELVKAQKLESVGI
HHHHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGGIAHDFNNILAAILGNVSLAKLYLSPDDPAYDKMTQAEKSCLQARELTGRLITFSKG
EECHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
GEPLRKKVAVAPFLKDAVSFFLSGSNVRCEFDFPDFLWPLEIDVGQMQQVVRHLIVNAQE
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHH
AMPDGGTIRISAENVSLKADEVPSLKEGPYVRICIKDQGVGIPREYQTRVFDPYFTTKPM
HCCCCCEEEEEECCEEEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHEEECCCCCCCCC
GGTKGAGLGLAICYSTIKKHEGFLSLVSQPGVETTINIYLPASSDDMKSVKP
CCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]