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The map label for this gene is entB [C]

Identifier: 85858643

GI number: 85858643

Start: 812976

End: 813212

Strand: Direct

Name: entB [C]

Synonym: SYN_01752

Alternate gene names: 85858643

Gene position: 812976-813212 (Clockwise)

Preceding gene: 85858641

Following gene: 85858647

Centisome position: 25.57

GC content: 48.52

Gene sequence:

>237_bases
ATGTTAACGTCCTGCGGTTTTGAAGAGATTATCATCACGGGAGTCATGACGAACTGCTGCTGCGAGACGACTGCCCGGGA
TGTCTTCATGCGGGATTATCGGGTCTTTTTTGTTTCGGATGCAACGGCGACGGTAAACGAGGATCTGCATCTTTCGACGT
TGAAGAATCTTGCCTTTGCCTTTGCTCATGTTCTGGATACGGAAAGCCTGTGCCGATGGGTGGATGAAAATTTTTAG

Upstream 100 bases:

>100_bases
GGGGTGCAGGATAATATTTATTATTTGAATTCTTCTGACAACCGAACAGATTATTGATAAAAACCGTTACAGCGCTTTCC
GGGGAACGGTTCTCGATGAA

Downstream 100 bases:

>100_bases
GATGTTTATTTCCTGCGCGGCGGAAGTGATGGCTTCAAATGTGTATCGTTTTCAAGAACCATGTTCAGATGGTCCACAAC
CCGGCGTTCGCAACTCTCAT

Product: isochorismate hydrolase

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: Isochorismatase-Related Protein; Isochorismate Hydrolase

Number of amino acids: Translated: 78; Mature: 78

Protein sequence:

>78_residues
MLTSCGFEEIIITGVMTNCCCETTARDVFMRDYRVFFVSDATATVNEDLHLSTLKNLAFAFAHVLDTESLCRWVDENF

Sequences:

>Translated_78_residues
MLTSCGFEEIIITGVMTNCCCETTARDVFMRDYRVFFVSDATATVNEDLHLSTLKNLAFAFAHVLDTESLCRWVDENF
>Mature_78_residues
MLTSCGFEEIIITGVMTNCCCETTARDVFMRDYRVFFVSDATATVNEDLHLSTLKNLAFAFAHVLDTESLCRWVDENF

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786811, Length=70, Percent_Identity=45.7142857142857, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 8880; Mature: 8880

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
10.3 %Cys+Met (Translated Protein)
6.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
10.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSCGFEEIIITGVMTNCCCETTARDVFMRDYRVFFVSDATATVNEDLHLSTLKNLAFA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHHHH
FAHVLDTESLCRWVDENF
HHHHHCHHHHHHHHCCCC
>Mature Secondary Structure
MLTSCGFEEIIITGVMTNCCCETTARDVFMRDYRVFFVSDATATVNEDLHLSTLKNLAFA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHHHH
FAHVLDTESLCRWVDENF
HHHHHCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA