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The map label for this gene is oprM [H]

Identifier: 85858611

GI number: 85858611

Start: 780755

End: 782305

Strand: Direct

Name: oprM [H]

Synonym: SYN_02946

Alternate gene names: 85858611

Gene position: 780755-782305 (Clockwise)

Preceding gene: 85858610

Following gene: 85858612

Centisome position: 24.56

GC content: 58.61

Gene sequence:

>1551_bases
TTGCCATCTTCCTCATCCCCGTCACGTTCACCGTTGTGGAAAAGCTGTCGCACCGGGAGGAAAGGGGCGCACAAATCCAG
GCTGAAGAGACTGAAAAGGAGGGGAAGGGCCATGAATAAATCGATCTTCGCCATCCTGATTGTCCTTGTTCTGGCTGGCT
GTGCCGTCGGTCCTGACTACAGGCGTCCGGTGATAGAAAGTCCTCCGGCCTGGCGCATCGATGAAGTTGAGGCACAGGAA
ACAGCCAATATGGCATGGTGGGATCAGCTCAACGATCCGGTCATGAACTCACTGATCGATGAGGCCCTGAAGCAGAACCT
GGATCTGAAGATCGCCACCGCCCGGATCGACGAGTACATCGGCAGGTACTGGATGGGACGCTCCGGCCTGTTCCCGCAAA
TCGGGGTGACCGCTGAAGCAGGGCGTAGCCGTTCCTCCGAGGAGGGAGCGGCCCCGATGTCGCCGCAGGTGGAAAATCCG
GCGGATTTTTACCAGGGCTTCTTCAGCGGCAGTTGGGAGATCGATCTCTGGGGTAAATTGAGAAGGGCCACCGAAGCATC
GCGGGCCGATCTGCTGGCCACGCAAGAGGCACGGCAGGCGGTCATCCTGTCGCTGGTCAGCGCCGTGGCGAACGGTTACA
TCACTTTGCGCGATTTCGATAAACAGAGAGAAATCGCCGTAAATACCGCTAAGACAAGAGAGGAATCCTACAAGCTATTC
AAACAGCGTTTTGAAGGCGGGGTCATTTCGGAACTCGAACTCAACCAGGTCAAGTCCGAGTATGAACAGGCCCTGGCCAC
GATACCGCAGATAGAGAAACAGATTGTCTTTCAGGAAAACGCCCTGAGCCGTTTGCTGGGAAGGAATCCCGGACCCATCG
CCCGGGGAAAGCACATAGACGAACTGGTCCTTCCCGCCGTCCCCATGGGCCTTCCCTCGGATCTGCTGGCTAATCGCCCG
GACATCCGCCAGGCGGAGCAGGTGCTGGTCGCGGCCAATGCCCGGATCGGAGTGGCAAGGGCGCAGTACTTTCCGGCACT
TTCTCTTACCGGCCTGTTCGGCCAGGCGAGCGCGGATCTCTCGAATCTGTTCACCGGATCAGCACGGGTGTGGAGCTGGG
GCGGAAGCGTAGCTGCTCCGATCTTCACGGGAGGAGCCATCAGAGGGCAGGTCAAGGCTGCCGAGGCCGTGCGGCAGCAG
GCCCTGTTCGGCTACCAGAGAGCCATTCAGACGGCCTTCCGGGAGGTCGAGGATGCCCTCGTCGATCAGCGGCGAACCCG
AGAACAGCTTGAGGCTCAGCAGCGGCAGGTCGATTCCCTGCGTGATTACACCCGCTTTGCCCGGCTGCGCTATGAAAACG
GCTACACCAGCTACATAGAGGTCCTCGATGCGGAGAGGAGTCTCTTCTCCGCGGAGCTTACCTACGTCCAGACCCAGGGG
ACCCTCTTTGGAGCGCTTGTAAATCTCTATAAAGCCATGGGCGGAGGATGGGTTGTCGAGGCGGATAAGCTGACGGTATC
TTCATCGAAAAAAGAAGGTTCATCCGGTTAA

Upstream 100 bases:

>100_bases
CTGGGCTGCGTGCCGCTGGCGATGGCAAGCGGCGCCGGAGCACTCTCGCGGCGGGTGATGGGCTGGGCCGTTATCGGCGG
GATGCTGGCCGCAAGTTTCA

Downstream 100 bases:

>100_bases
TGAGTACCTTGGTATTGCATAAAATCACATGGACATGGCAAGAGCCCTGTTGTTTGAGACCAATCGAACAACAAAAGGAG
GCTCCAGACCATGTCCACAT

Product: type I secretion outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MPSSSSPSRSPLWKSCRTGRKGAHKSRLKRLKRRGRAMNKSIFAILIVLVLAGCAVGPDYRRPVIESPPAWRIDEVEAQE
TANMAWWDQLNDPVMNSLIDEALKQNLDLKIATARIDEYIGRYWMGRSGLFPQIGVTAEAGRSRSSEEGAAPMSPQVENP
ADFYQGFFSGSWEIDLWGKLRRATEASRADLLATQEARQAVILSLVSAVANGYITLRDFDKQREIAVNTAKTREESYKLF
KQRFEGGVISELELNQVKSEYEQALATIPQIEKQIVFQENALSRLLGRNPGPIARGKHIDELVLPAVPMGLPSDLLANRP
DIRQAEQVLVAANARIGVARAQYFPALSLTGLFGQASADLSNLFTGSARVWSWGGSVAAPIFTGGAIRGQVKAAEAVRQQ
ALFGYQRAIQTAFREVEDALVDQRRTREQLEAQQRQVDSLRDYTRFARLRYENGYTSYIEVLDAERSLFSAELTYVQTQG
TLFGALVNLYKAMGGGWVVEADKLTVSSSKKEGSSG

Sequences:

>Translated_516_residues
MPSSSSPSRSPLWKSCRTGRKGAHKSRLKRLKRRGRAMNKSIFAILIVLVLAGCAVGPDYRRPVIESPPAWRIDEVEAQE
TANMAWWDQLNDPVMNSLIDEALKQNLDLKIATARIDEYIGRYWMGRSGLFPQIGVTAEAGRSRSSEEGAAPMSPQVENP
ADFYQGFFSGSWEIDLWGKLRRATEASRADLLATQEARQAVILSLVSAVANGYITLRDFDKQREIAVNTAKTREESYKLF
KQRFEGGVISELELNQVKSEYEQALATIPQIEKQIVFQENALSRLLGRNPGPIARGKHIDELVLPAVPMGLPSDLLANRP
DIRQAEQVLVAANARIGVARAQYFPALSLTGLFGQASADLSNLFTGSARVWSWGGSVAAPIFTGGAIRGQVKAAEAVRQQ
ALFGYQRAIQTAFREVEDALVDQRRTREQLEAQQRQVDSLRDYTRFARLRYENGYTSYIEVLDAERSLFSAELTYVQTQG
TLFGALVNLYKAMGGGWVVEADKLTVSSSKKEGSSG
>Mature_515_residues
PSSSSPSRSPLWKSCRTGRKGAHKSRLKRLKRRGRAMNKSIFAILIVLVLAGCAVGPDYRRPVIESPPAWRIDEVEAQET
ANMAWWDQLNDPVMNSLIDEALKQNLDLKIATARIDEYIGRYWMGRSGLFPQIGVTAEAGRSRSSEEGAAPMSPQVENPA
DFYQGFFSGSWEIDLWGKLRRATEASRADLLATQEARQAVILSLVSAVANGYITLRDFDKQREIAVNTAKTREESYKLFK
QRFEGGVISELELNQVKSEYEQALATIPQIEKQIVFQENALSRLLGRNPGPIARGKHIDELVLPAVPMGLPSDLLANRPD
IRQAEQVLVAANARIGVARAQYFPALSLTGLFGQASADLSNLFTGSARVWSWGGSVAAPIFTGGAIRGQVKAAEAVRQQA
LFGYQRAIQTAFREVEDALVDQRRTREQLEAQQRQVDSLRDYTRFARLRYENGYTSYIEVLDAERSLFSAELTYVQTQGT
LFGALVNLYKAMGGGWVVEADKLTVSSSKKEGSSG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=463, Percent_Identity=34.341252699784, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI2367348, Length=424, Percent_Identity=23.8207547169811, Blast_Score=115, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 57187; Mature: 57055

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSSSPSRSPLWKSCRTGRKGAHKSRLKRLKRRGRAMNKSIFAILIVLVLAGCAVGPDY
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RRPVIESPPAWRIDEVEAQETANMAWWDQLNDPVMNSLIDEALKQNLDLKIATARIDEYI
CCCCCCCCCCCCCCCCHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
GRYWMGRSGLFPQIGVTAEAGRSRSSEEGAAPMSPQVENPADFYQGFFSGSWEIDLWGKL
HHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHH
RRATEASRADLLATQEARQAVILSLVSAVANGYITLRDFDKQREIAVNTAKTREESYKLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
KQRFEGGVISELELNQVKSEYEQALATIPQIEKQIVFQENALSRLLGRNPGPIARGKHID
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
ELVLPAVPMGLPSDLLANRPDIRQAEQVLVAANARIGVARAQYFPALSLTGLFGQASADL
HHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHCCHHHHH
SNLFTGSARVWSWGGSVAAPIFTGGAIRGQVKAAEAVRQQALFGYQRAIQTAFREVEDAL
HHHHCCCCEEEECCCCEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDQRRTREQLEAQQRQVDSLRDYTRFARLRYENGYTSYIEVLDAERSLFSAELTYVQTQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECC
TLFGALVNLYKAMGGGWVVEADKLTVSSSKKEGSSG
HHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCC
>Mature Secondary Structure 
PSSSSPSRSPLWKSCRTGRKGAHKSRLKRLKRRGRAMNKSIFAILIVLVLAGCAVGPDY
CCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RRPVIESPPAWRIDEVEAQETANMAWWDQLNDPVMNSLIDEALKQNLDLKIATARIDEYI
CCCCCCCCCCCCCCCCHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHH
GRYWMGRSGLFPQIGVTAEAGRSRSSEEGAAPMSPQVENPADFYQGFFSGSWEIDLWGKL
HHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHH
RRATEASRADLLATQEARQAVILSLVSAVANGYITLRDFDKQREIAVNTAKTREESYKLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
KQRFEGGVISELELNQVKSEYEQALATIPQIEKQIVFQENALSRLLGRNPGPIARGKHID
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
ELVLPAVPMGLPSDLLANRPDIRQAEQVLVAANARIGVARAQYFPALSLTGLFGQASADL
HHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHCCHHHHH
SNLFTGSARVWSWGGSVAAPIFTGGAIRGQVKAAEAVRQQALFGYQRAIQTAFREVEDAL
HHHHCCCCEEEECCCCEECHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDQRRTREQLEAQQRQVDSLRDYTRFARLRYENGYTSYIEVLDAERSLFSAELTYVQTQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECC
TLFGALVNLYKAMGGGWVVEADKLTVSSSKKEGSSG
HHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]