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The map label for this gene is yfkN [H]

Identifier: 85858427

GI number: 85858427

Start: 577845

End: 579506

Strand: Direct

Name: yfkN [H]

Synonym: SYN_02264

Alternate gene names: 85858427

Gene position: 577845-579506 (Clockwise)

Preceding gene: 85858426

Following gene: 85858428

Centisome position: 18.18

GC content: 57.28

Gene sequence:

>1662_bases
ATGATCGGTTGGAGGGTGCGTTTCGAGATGGAATCGGAGAGAGAAAAGGGAGTTGATTCCAGGCGTTTAAAAGAGGGGGG
GAGTTTTTGTAGAACCTTGAAAAAACTTCGTTTGACAGCCGTCCTGGTTTTTGCGCTGCTGAGTTTTTCCTTTACGGGTT
TCGTTTCTCAGGCCAATGCGGGGGAAAAGCGAATCCGGATTCTGTATGTCAATGATTTCCATGGCTTTTCACAACCTTAC
CGCCCCTATGGCATGGATGACCAGTGGGGTGGTGCGGCCTTTCTCGATACGAAGATCCGGGCGCTGCGTGCCGATCCTTC
CATTCCGACTCTGCTGCTGGCCGCCGGAGACATGATTCAGGGAAACAACTGGGCCAATCTTTTTCAGGGAAGGTCGGTCA
TCGAACTGATGAATCTCATGGCCTTCGATGCCATGGTGATGGGCAATCACGAGTTGGATTTCGGCCAGGACGTTCTGAAA
CAGCGGATCAGAGAAGCGGCATTTCCTGTTCTGGCAGCCAATGTTCTGGGGCTCCCGGAACTGAAGCCTTACATGATCAA
GGAACTCGACGGGATTAAGGTAGCAGTGATCGGCCTGGTGACGGAGCATACACCCACGTGCACCCACCCGGCGAATGCCG
TGGGACTGACCTTTCTTTCCCCGATAAAAGTTCTGAAGACTTATCTTCCGGAGCTGCGGCGTACCGCTGACGTGGTGATC
GTTCTGAGCCATCTCGGCCATGACGTGGATCGGTTTCTTGCGGGACAGGTGTCCGGAGTGGATGCCATAATCGGTGGACA
TTCCCACACACGGGTGGCAAAGCCTCCCTGCATCGGCAATACGCTGGTCCTCCAGGCCTGGGAGCACGGCAGGGCGCTGG
GCGTTCTGGATTTAACCGTGGAAGAGGGGCGGGTCACGGATTCCAGTTCCCGCCTGATAGATGTGAAGCCGGAGGATGGA
GCTGCCGGTGAGTCTGTGGCTGAGCTTGTCCAACGGTATCAGCAGCGGATGGATGCGGTGTTGGATTCGGTTGTCGGCCG
GACCCGGGTCAGTCTCAACGGGGCTGAAGTGCGGTTTCGGGAAACCAATTTTGGCAATCTTGTGGCCGACGTTGTCCGGC
GGACTGCGGGGGCGGAGGTTGCTCTGATCAATGGGGGGACGATTCGCGCGAGCATTCGGAAAGGGCCCGTCCGGCTGAAG
GAACTCTATTCCGCCCTGCCGTTCAACAATTACGTAGTGGCGTTCCGGCTGCGTGGGGACCAGCTTCGAGCCGCACTGGA
GCATGGCCTTGCCCGGGTTGAATTTCGCGATGGCGCCTTTCCGCAGATATCGGGAATGACCCTGACCTATGACCCGTCAG
CACCGGCAGGGCGGCGGATAAAGACGCTTTTCGTGGGCGGGCAGGCGCTGGAGGCGGGAAGAGAGTATACCGTGGCGACG
AATGATTTTCTGGCGGCGGGTGGCGATGGATATAAGGCTTTTGCGGAAGTCATTCATGCTGAAGACCTCGTTGAACCGTC
TGCTGCAGGCCGGCCGGGAGCGAAGCTCGTATTCAATGATGCCGGTCGCTGGCTGCGGGATCTTGTGGCCGACACGCTCC
GGACGCAGGGTGAAATCCACCCGGTTCGCGAAAACCGCATCGTGGAGATTTTTCATCCTTGA

Upstream 100 bases:

>100_bases
AAGACACGGGAATCGGCCCTGTTCAGATGTTCCGGGCGGTTCGGACATTCAAGATCGACGCCCGGTTTCTCCTTGATGAA
AACCTGCCTGAAGATCGGAA

Downstream 100 bases:

>100_bases
AGATTTATGTCTGCAATCAACCCGTGTTTGTCGCCGCGGGCATGACCGTCCGTCACGCCCTGCTGGCGGCGGGATGCCGG
GAAAAGCCGGAGGAACTTCT

Product: UDP-sugar diphosphatase / 5'-nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MIGWRVRFEMESEREKGVDSRRLKEGGSFCRTLKKLRLTAVLVFALLSFSFTGFVSQANAGEKRIRILYVNDFHGFSQPY
RPYGMDDQWGGAAFLDTKIRALRADPSIPTLLLAAGDMIQGNNWANLFQGRSVIELMNLMAFDAMVMGNHELDFGQDVLK
QRIREAAFPVLAANVLGLPELKPYMIKELDGIKVAVIGLVTEHTPTCTHPANAVGLTFLSPIKVLKTYLPELRRTADVVI
VLSHLGHDVDRFLAGQVSGVDAIIGGHSHTRVAKPPCIGNTLVLQAWEHGRALGVLDLTVEEGRVTDSSSRLIDVKPEDG
AAGESVAELVQRYQQRMDAVLDSVVGRTRVSLNGAEVRFRETNFGNLVADVVRRTAGAEVALINGGTIRASIRKGPVRLK
ELYSALPFNNYVVAFRLRGDQLRAALEHGLARVEFRDGAFPQISGMTLTYDPSAPAGRRIKTLFVGGQALEAGREYTVAT
NDFLAAGGDGYKAFAEVIHAEDLVEPSAAGRPGAKLVFNDAGRWLRDLVADTLRTQGEIHPVRENRIVEIFHP

Sequences:

>Translated_553_residues
MIGWRVRFEMESEREKGVDSRRLKEGGSFCRTLKKLRLTAVLVFALLSFSFTGFVSQANAGEKRIRILYVNDFHGFSQPY
RPYGMDDQWGGAAFLDTKIRALRADPSIPTLLLAAGDMIQGNNWANLFQGRSVIELMNLMAFDAMVMGNHELDFGQDVLK
QRIREAAFPVLAANVLGLPELKPYMIKELDGIKVAVIGLVTEHTPTCTHPANAVGLTFLSPIKVLKTYLPELRRTADVVI
VLSHLGHDVDRFLAGQVSGVDAIIGGHSHTRVAKPPCIGNTLVLQAWEHGRALGVLDLTVEEGRVTDSSSRLIDVKPEDG
AAGESVAELVQRYQQRMDAVLDSVVGRTRVSLNGAEVRFRETNFGNLVADVVRRTAGAEVALINGGTIRASIRKGPVRLK
ELYSALPFNNYVVAFRLRGDQLRAALEHGLARVEFRDGAFPQISGMTLTYDPSAPAGRRIKTLFVGGQALEAGREYTVAT
NDFLAAGGDGYKAFAEVIHAEDLVEPSAAGRPGAKLVFNDAGRWLRDLVADTLRTQGEIHPVRENRIVEIFHP
>Mature_553_residues
MIGWRVRFEMESEREKGVDSRRLKEGGSFCRTLKKLRLTAVLVFALLSFSFTGFVSQANAGEKRIRILYVNDFHGFSQPY
RPYGMDDQWGGAAFLDTKIRALRADPSIPTLLLAAGDMIQGNNWANLFQGRSVIELMNLMAFDAMVMGNHELDFGQDVLK
QRIREAAFPVLAANVLGLPELKPYMIKELDGIKVAVIGLVTEHTPTCTHPANAVGLTFLSPIKVLKTYLPELRRTADVVI
VLSHLGHDVDRFLAGQVSGVDAIIGGHSHTRVAKPPCIGNTLVLQAWEHGRALGVLDLTVEEGRVTDSSSRLIDVKPEDG
AAGESVAELVQRYQQRMDAVLDSVVGRTRVSLNGAEVRFRETNFGNLVADVVRRTAGAEVALINGGTIRASIRKGPVRLK
ELYSALPFNNYVVAFRLRGDQLRAALEHGLARVEFRDGAFPQISGMTLTYDPSAPAGRRIKTLFVGGQALEAGREYTVAT
NDFLAAGGDGYKAFAEVIHAEDLVEPSAAGRPGAKLVFNDAGRWLRDLVADTLRTQGEIHPVRENRIVEIFHP

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=572, Percent_Identity=28.4965034965035, Blast_Score=173, Evalue=4e-43,
Organism=Escherichia coli, GI1786687, Length=515, Percent_Identity=28.7378640776699, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1790658, Length=550, Percent_Identity=22.7272727272727, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921980, Length=456, Percent_Identity=28.0701754385965, Blast_Score=169, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24652512, Length=456, Percent_Identity=28.0701754385965, Blast_Score=169, Evalue=6e-42,
Organism=Drosophila melanogaster, GI161076508, Length=456, Percent_Identity=28.0701754385965, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI28573524, Length=490, Percent_Identity=27.7551020408163, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI19922444, Length=506, Percent_Identity=28.8537549407115, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19922446, Length=529, Percent_Identity=27.7882797731569, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24654424, Length=529, Percent_Identity=27.7882797731569, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24641187, Length=506, Percent_Identity=26.6798418972332, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI221329836, Length=511, Percent_Identity=26.8101761252446, Blast_Score=138, Evalue=1e-32,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 60675; Mature: 60675

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGWRVRFEMESEREKGVDSRRLKEGGSFCRTLKKLRLTAVLVFALLSFSFTGFVSQANA
CCCEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GEKRIRILYVNDFHGFSQPYRPYGMDDQWGGAAFLDTKIRALRADPSIPTLLLAAGDMIQ
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHEECCCCCCEEEEECCCEEC
GNNWANLFQGRSVIELMNLMAFDAMVMGNHELDFGQDVLKQRIREAAFPVLAANVLGLPE
CCCHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
LKPYMIKELDGIKVAVIGLVTEHTPTCTHPANAVGLTFLSPIKVLKTYLPELRRTADVVI
CCHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
VLSHLGHDVDRFLAGQVSGVDAIIGGHSHTRVAKPPCIGNTLVLQAWEHGRALGVLDLTV
EEHHHCCCHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEE
EEGRVTDSSSRLIDVKPEDGAAGESVAELVQRYQQRMDAVLDSVVGRTRVSLNGAEVRFR
ECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEE
ETNFGNLVADVVRRTAGAEVALINGGTIRASIRKGPVRLKELYSALPFNNYVVAFRLRGD
ECCCHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCH
QLRAALEHGLARVEFRDGAFPQISGMTLTYDPSAPAGRRIKTLFVGGQALEAGREYTVAT
HHHHHHHCCCEEEEECCCCCCEECCEEEEECCCCCCCCEEEEEEECCHHHHCCCCEEEEE
NDFLAAGGDGYKAFAEVIHAEDLVEPSAAGRPGAKLVFNDAGRWLRDLVADTLRTQGEIH
CCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEE
PVRENRIVEIFHP
ECCCCCEEEEECC
>Mature Secondary Structure
MIGWRVRFEMESEREKGVDSRRLKEGGSFCRTLKKLRLTAVLVFALLSFSFTGFVSQANA
CCCEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GEKRIRILYVNDFHGFSQPYRPYGMDDQWGGAAFLDTKIRALRADPSIPTLLLAAGDMIQ
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHEECCCCCCEEEEECCCEEC
GNNWANLFQGRSVIELMNLMAFDAMVMGNHELDFGQDVLKQRIREAAFPVLAANVLGLPE
CCCHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
LKPYMIKELDGIKVAVIGLVTEHTPTCTHPANAVGLTFLSPIKVLKTYLPELRRTADVVI
CCHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
VLSHLGHDVDRFLAGQVSGVDAIIGGHSHTRVAKPPCIGNTLVLQAWEHGRALGVLDLTV
EEHHHCCCHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEEE
EEGRVTDSSSRLIDVKPEDGAAGESVAELVQRYQQRMDAVLDSVVGRTRVSLNGAEVRFR
ECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEE
ETNFGNLVADVVRRTAGAEVALINGGTIRASIRKGPVRLKELYSALPFNNYVVAFRLRGD
ECCCHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCH
QLRAALEHGLARVEFRDGAFPQISGMTLTYDPSAPAGRRIKTLFVGGQALEAGREYTVAT
HHHHHHHCCCEEEEECCCCCCEECCEEEEECCCCCCCCEEEEEEECCHHHHCCCCEEEEE
NDFLAAGGDGYKAFAEVIHAEDLVEPSAAGRPGAKLVFNDAGRWLRDLVADTLRTQGEIH
CCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEE
PVRENRIVEIFHP
ECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]