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The map label for this gene is hemD [H]

Identifier: 85858419

GI number: 85858419

Start: 569353

End: 570903

Strand: Direct

Name: hemD [H]

Synonym: SYN_02272

Alternate gene names: 85858419

Gene position: 569353-570903 (Clockwise)

Preceding gene: 85858418

Following gene: 85858420

Centisome position: 17.91

GC content: 60.8

Gene sequence:

>1551_bases
ATGGGGCAGGGGACGGATTCGATGAGCAAAAGGGGGAGAGTTTACATTATTGGCGCGGGACCGGGAGATCCGGGACTGAT
GACGGTGAAGGGAACGAAATGCCTTGCGGAAGCGGATGTCGTGATCTACGACCATCTGATCAATCCGGGAATCCTTCATC
ATGCCCGCGAAACCGCTCGTCTGGTGTATGCAGGAAAAGAAGGAGGAAAACATACCCTGTCACAGGAAGAGATCAACGCG
AGGCTGGTGTCGGAAGCTCAGCAGGGGCATGTCGTGGCCCGGCTGAAAGGGGGAGATCCCTTCATCTTCGGGCGTGGAGG
GGAAGAGGCGGAGGTGCTGGCCCGTGAGGGCATCCCCTTTGAAATCGTCCCCGGTGTGACTTCGGCGATCGCCGTCGCTT
CCTATGCGGGCATCCCGCTGACTCATCGCCGCCATACCGCCACCCTGGCCTTTGTCACGGGACACGAAGATCCTTTGAAA
GGACAGAGCGAGATCGACTGGAAGGCCCTGGCCGGAATAGGGACTCTTGTTTTTCTCATGGGGGTCCGCAATCTGTCCCG
CATTGCGGCCGGCCTGATCGCCCAGGGGAAAAAGCCGGAAACTCCCGCCGCGCTGATCCGCTGGGGAACGACGGCGAATC
AGGAAACGCTTGTCGGTACGCTGGAGAATATCGCCGACCTTGCCGAGGCAGCCGGATTTGCGCCGCCGGCCATCCTGGTG
GTCGGCGGGGTCGTGGGACTGCGGAAGGAACTGAACTGGTTTGAGACCAGGCCCCTCTTTGGAAAGGGGATCGTGATTAC
CCGTCCCGAATCCCAGGCGGAATCACTGGCCGCACTTCTTGAGGACGCCGGAGCGCGGGTCATTTCCTTTCCCACCATCC
AGATCGTTGCCCCGGAGAGCTGGGAGGGACTGGATTCGGCAATTCGCCGTCTGGAGGATTACGCCTGGCTGATCTTCACC
AGCGCCAACGGTGTGCGCTTTTTTCTGGAGCGGCTTTACGTCACGGGAAAAGATGTGCGGGATCTCAAGGGAATACGGCT
GTGCACCATCGGGCCCGCCACGGCGGCGGCGCTGGAAAACCGCGGTCTCCGGGTGGATCTGGTTCCGGAAACCTACCTGT
CCGAAGGGGTGGTGGCGGCCTTCGAGCGGGTTGCGATTTCAGGGCAGAGGATACTCCTTCCCCGGGCGGAGAAGGCCCGC
GATGTCATTCCAGAGGGGCTGACCCGTCAGGGAGCGGTCGTGGATGTCGTTTCGGCATACCGCACGGTGAATTCCGGCCG
GGATCGTTCGGAGCTGGTGGAGCTGATTGCCCAGAGGCGGGTCGATGTCCTGACCTTTACGAGCCCATCCACAGTCACCA
ATTTTCTGGAGATCATGGGGCCGGATTTTCTTCCCCTGCCCGCCGGCATCCGGGTGGCCTGCATCGGACCAGTGACTGCG
GCCGCCGCTGAAAAAGCCGGCCTGCCCGTGGATATCCTTCAGGAAATCTACACCATTCCTGGGCTGGTCGAATCGCTCAA
GGTCTTTCTGGCAAAGGATAAGCCGCATTGA

Upstream 100 bases:

>100_bases
CTGGAACACCTGGAGGATGGGCAGCCCTGATCAATCCATTTCAATATAGCAGAATCGCCGCATTCCGTGGTGGGAGCGCA
TTTTCAATAAAGGCCTCAAA

Downstream 100 bases:

>100_bases
CAGCCTCTTTCTTATATGTTTCCCGTAACAATGTACCTGAAATATAGGGGATGGCAATTCCTGTGAGGAGGGCAACTCAT
GATGGAAAATTTTCCAAAAC

Product: uroporphyrin-III C-methyltransferase / uroporphyrinogen-III synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MGQGTDSMSKRGRVYIIGAGPGDPGLMTVKGTKCLAEADVVIYDHLINPGILHHARETARLVYAGKEGGKHTLSQEEINA
RLVSEAQQGHVVARLKGGDPFIFGRGGEEAEVLAREGIPFEIVPGVTSAIAVASYAGIPLTHRRHTATLAFVTGHEDPLK
GQSEIDWKALAGIGTLVFLMGVRNLSRIAAGLIAQGKKPETPAALIRWGTTANQETLVGTLENIADLAEAAGFAPPAILV
VGGVVGLRKELNWFETRPLFGKGIVITRPESQAESLAALLEDAGARVISFPTIQIVAPESWEGLDSAIRRLEDYAWLIFT
SANGVRFFLERLYVTGKDVRDLKGIRLCTIGPATAAALENRGLRVDLVPETYLSEGVVAAFERVAISGQRILLPRAEKAR
DVIPEGLTRQGAVVDVVSAYRTVNSGRDRSELVELIAQRRVDVLTFTSPSTVTNFLEIMGPDFLPLPAGIRVACIGPVTA
AAAEKAGLPVDILQEIYTIPGLVESLKVFLAKDKPH

Sequences:

>Translated_516_residues
MGQGTDSMSKRGRVYIIGAGPGDPGLMTVKGTKCLAEADVVIYDHLINPGILHHARETARLVYAGKEGGKHTLSQEEINA
RLVSEAQQGHVVARLKGGDPFIFGRGGEEAEVLAREGIPFEIVPGVTSAIAVASYAGIPLTHRRHTATLAFVTGHEDPLK
GQSEIDWKALAGIGTLVFLMGVRNLSRIAAGLIAQGKKPETPAALIRWGTTANQETLVGTLENIADLAEAAGFAPPAILV
VGGVVGLRKELNWFETRPLFGKGIVITRPESQAESLAALLEDAGARVISFPTIQIVAPESWEGLDSAIRRLEDYAWLIFT
SANGVRFFLERLYVTGKDVRDLKGIRLCTIGPATAAALENRGLRVDLVPETYLSEGVVAAFERVAISGQRILLPRAEKAR
DVIPEGLTRQGAVVDVVSAYRTVNSGRDRSELVELIAQRRVDVLTFTSPSTVTNFLEIMGPDFLPLPAGIRVACIGPVTA
AAAEKAGLPVDILQEIYTIPGLVESLKVFLAKDKPH
>Mature_515_residues
GQGTDSMSKRGRVYIIGAGPGDPGLMTVKGTKCLAEADVVIYDHLINPGILHHARETARLVYAGKEGGKHTLSQEEINAR
LVSEAQQGHVVARLKGGDPFIFGRGGEEAEVLAREGIPFEIVPGVTSAIAVASYAGIPLTHRRHTATLAFVTGHEDPLKG
QSEIDWKALAGIGTLVFLMGVRNLSRIAAGLIAQGKKPETPAALIRWGTTANQETLVGTLENIADLAEAAGFAPPAILVV
GGVVGLRKELNWFETRPLFGKGIVITRPESQAESLAALLEDAGARVISFPTIQIVAPESWEGLDSAIRRLEDYAWLIFTS
ANGVRFFLERLYVTGKDVRDLKGIRLCTIGPATAAALENRGLRVDLVPETYLSEGVVAAFERVAISGQRILLPRAEKARD
VIPEGLTRQGAVVDVVSAYRTVNSGRDRSELVELIAQRRVDVLTFTSPSTVTNFLEIMGPDFLPLPAGIRVACIGPVTAA
AAEKAGLPVDILQEIYTIPGLVESLKVFLAKDKPH

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=253, Percent_Identity=46.2450592885375, Blast_Score=233, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6322922, Length=251, Percent_Identity=31.0756972111554, Blast_Score=113, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 55211; Mature: 55080

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQGTDSMSKRGRVYIIGAGPGDPGLMTVKGTKCLAEADVVIYDHLINPGILHHARETAR
CCCCCCCHHHCCEEEEEECCCCCCCEEEEECCCCHHHCCEEEEEHHCCCHHHHHHHHHEE
LVYAGKEGGKHTLSQEEINARLVSEAQQGHVVARLKGGDPFIFGRGGEEAEVLAREGIPF
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHCCCCE
EIVPGVTSAIAVASYAGIPLTHRRHTATLAFVTGHEDPLKGQSEIDWKALAGIGTLVFLM
EECCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
GVRNLSRIAAGLIAQGKKPETPAALIRWGTTANQETLVGTLENIADLAEAAGFAPPAILV
HHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
VGGVVGLRKELNWFETRPLFGKGIVITRPESQAESLAALLEDAGARVISFPTIQIVAPES
HHHHHHHHHHCCHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEECCEEEEECCCC
WEGLDSAIRRLEDYAWLIFTSANGVRFFLERLYVTGKDVRDLKGIRLCTIGPATAAALEN
CCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCHHHCCCEEEEEECCHHHHHHHC
RGLRVDLVPETYLSEGVVAAFERVAISGQRILLPRAEKARDVIPEGLTRQGAVVDVVSAY
CCCEEEECCHHHHHCHHHHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCCCHHHHHHHHH
RTVNSGRDRSELVELIAQRRVDVLTFTSPSTVTNFLEIMGPDFLPLPAGIRVACIGPVTA
HHHCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEECCHHH
AAAEKAGLPVDILQEIYTIPGLVESLKVFLAKDKPH
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GQGTDSMSKRGRVYIIGAGPGDPGLMTVKGTKCLAEADVVIYDHLINPGILHHARETAR
CCCCCCHHHCCEEEEEECCCCCCCEEEEECCCCHHHCCEEEEEHHCCCHHHHHHHHHEE
LVYAGKEGGKHTLSQEEINARLVSEAQQGHVVARLKGGDPFIFGRGGEEAEVLAREGIPF
EEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHCCCCE
EIVPGVTSAIAVASYAGIPLTHRRHTATLAFVTGHEDPLKGQSEIDWKALAGIGTLVFLM
EECCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
GVRNLSRIAAGLIAQGKKPETPAALIRWGTTANQETLVGTLENIADLAEAAGFAPPAILV
HHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
VGGVVGLRKELNWFETRPLFGKGIVITRPESQAESLAALLEDAGARVISFPTIQIVAPES
HHHHHHHHHHCCHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEECCEEEEECCCC
WEGLDSAIRRLEDYAWLIFTSANGVRFFLERLYVTGKDVRDLKGIRLCTIGPATAAALEN
CCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCHHHCCCEEEEEECCHHHHHHHC
RGLRVDLVPETYLSEGVVAAFERVAISGQRILLPRAEKARDVIPEGLTRQGAVVDVVSAY
CCCEEEECCHHHHHCHHHHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCCCHHHHHHHHH
RTVNSGRDRSELVELIAQRRVDVLTFTSPSTVTNFLEIMGPDFLPLPAGIRVACIGPVTA
HHHCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEECCHHH
AAAEKAGLPVDILQEIYTIPGLVESLKVFLAKDKPH
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]