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The map label for this gene is cwlK [H]

Identifier: 85858381

GI number: 85858381

Start: 538375

End: 538773

Strand: Direct

Name: cwlK [H]

Synonym: SYN_02309

Alternate gene names: 85858381

Gene position: 538375-538773 (Clockwise)

Preceding gene: 85858380

Following gene: 85858382

Centisome position: 16.93

GC content: 60.15

Gene sequence:

>399_bases
ATGAGCCGCCTGATTTCCGACTGCCTTCCCGAACTCCAGGAGAAGGCGGAAGCCTTTTCCGTGGCGATGGTCAACGCGGG
GATCCCTTTCATGTACACCTGCACCAGGCGGACACAGGAAGAGCAGGACGCCCTCTATGCCCAGGGGCGGACGAAGCCGG
GAAAGATAGTCACCTGGACGCGCAAGTCCAAGCATATCGACGGCAAGGCCTTCGACATCGCCATCCTCAGGGATTTCAAG
CCCTGCTGGGATGTGAAGGTCGACGTGAACGAAAACGAGATTCCCGATTACGAGGAAGCGGGCCGGATCGGCGAATCCGT
GGGTCTGCGCTGGGGCGGGAGATTCAGGAACAGCAAGGGGAAGCCGCGCCCGGATTATCCCCACTTCGAGATCGCTTAG

Upstream 100 bases:

>100_bases
CCCGGCAGGCAAGAAAGGGGCTGAATTCGGACGACTGCCTGAAGATCAAGCATTATGCGGACATCCTGATCTCATCCCTG
TCGGTGAAGGAGCCCAAGCC

Downstream 100 bases:

>100_bases
GAGGAAACAATGAGCAGATGGTTCTGTGTGAAGGAAAGCGTTGAACCGGCCGTCTCCATGCTGGCCTTTGTGGCCGGCGG
GCTGGTGAAGGCCAACAATC

Product: L-alanyl-D-glutamate peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MSRLISDCLPELQEKAEAFSVAMVNAGIPFMYTCTRRTQEEQDALYAQGRTKPGKIVTWTRKSKHIDGKAFDIAILRDFK
PCWDVKVDVNENEIPDYEEAGRIGESVGLRWGGRFRNSKGKPRPDYPHFEIA

Sequences:

>Translated_132_residues
MSRLISDCLPELQEKAEAFSVAMVNAGIPFMYTCTRRTQEEQDALYAQGRTKPGKIVTWTRKSKHIDGKAFDIAILRDFK
PCWDVKVDVNENEIPDYEEAGRIGESVGLRWGGRFRNSKGKPRPDYPHFEIA
>Mature_131_residues
SRLISDCLPELQEKAEAFSVAMVNAGIPFMYTCTRRTQEEQDALYAQGRTKPGKIVTWTRKSKHIDGKAFDIAILRDFKP
CWDVKVDVNENEIPDYEEAGRIGESVGLRWGGRFRNSKGKPRPDYPHFEIA

Specific function: Cleaves the linkage of the L-alanine-D-glutamic acid of B.subtilis cell wall [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR012340
- InterPro:   IPR003709 [H]

Pfam domain/function: PF02557 VanY [H]

EC number: NA

Molecular weight: Translated: 15093; Mature: 14962

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLISDCLPELQEKAEAFSVAMVNAGIPFMYTCTRRTQEEQDALYAQGRTKPGKIVTWT
CCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCEEEEE
RKSKHIDGKAFDIAILRDFKPCWDVKVDVNENEIPDYEEAGRIGESVGLRWGGRFRNSKG
CCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHCCCHHCCCCCCCCCCCCCC
KPRPDYPHFEIA
CCCCCCCCEECC
>Mature Secondary Structure 
SRLISDCLPELQEKAEAFSVAMVNAGIPFMYTCTRRTQEEQDALYAQGRTKPGKIVTWT
CHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCEEEEE
RKSKHIDGKAFDIAILRDFKPCWDVKVDVNENEIPDYEEAGRIGESVGLRWGGRFRNSKG
CCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHCCCHHCCCCCCCCCCCCCC
KPRPDYPHFEIA
CCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]