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The map label for this gene is tuf

Identifier: 85858144

GI number: 85858144

Start: 312244

End: 313437

Strand: Direct

Name: tuf

Synonym: SYN_00983

Alternate gene names: 85858144

Gene position: 312244-313437 (Clockwise)

Preceding gene: 85858143

Following gene: 85858145

Centisome position: 9.82

GC content: 49.75

Gene sequence:

>1194_bases
ATGGCTAAGAAGAAATTTGAAAGGACAAAGCCGCATTTAAATATTGGCACCATTGGACATGTCGATCATGGCAAGACAAC
TTTGACTGCTGCGATAACAAAGTGGCTTGCAAAGAAAGGCTTAGCCGAATTTCGAGCCTTTGATTCGATTGACAATGCAC
CGGAAGAAAAAGAAAGAGGCGTCACCATCAACATTTCCCATGTGGAATATCAGACGGACAAGCGTCATTATGCCCATGTG
GACTGTCCGGGGCACGCGGACTATATTAAAAATATGATCTCCGGTGCAGCGCACATGGATGGCACGATACTGGTCGTGGC
AGCTTCCGATGGTCCGATGCCGCAGACGCGAGAGCATATCCTCCTTGCACGTCAGGTGCAGGTTCCCTCTATCGTCGTTT
TTCTGAACAAAGTAGACCTTGTCGACGATCCGGAACTTCTTGATCTCGTTGAACTGGAGTTGAGAGAACTGCTGAATGAA
TACGAGTTTCCCGGGGACGATATTCCCATCATCAGAGGCTCCGCTTTAAAGGCCCTTGAAAGCGAAAATCCGGATGATCC
CGATGTAAAACAGATATATGCCCTGATGGATGCCGTGGATGCTTATATCCCTGAGCCTGAGCGTGATCTTGACAAGCCGT
TTCTGATGCCCGTGGGCGACGTTTTTACGATTTCAGGTCGCGGTACAGTTGTGACTGGTCGAATTGACCGGGGAATTATA
AAGACCGGTGATGAAGTCGAAATAGTCGGCGTTCGCCCGACACAAAAGACAGTTTGCACAGGTGTGGAAATGTTTCGCAA
GACCCTGGATGAAGGGCGTGCCGGTGACGATGTCGGCTTGCTGCTTCGGGGAATCAAGAGGGAAGACGTTGAAAGAGGCC
AGGTTGTTGCAAAACCGGGTTCTATCACGCCGCATACCAAATTTATGGCCCAAGTCTACGTTTTGACTAAGGAGGAAGGT
GGAAGGCATACTCCTTTCTTCACCGGCTATCGCCCGCAGTTTTATTTCAGAACAACCGATGTTACCGGAGTTGCCAAGTT
GGCGGAAGGTGTGGAAATGGTTATGCCGGGGGACAATGTAGAAATGGAAGTGACGTTGATCACGCCGATAGCCATGGAAG
AACAGCTGCGGTTCGCTATTCGCGAAGGCGGAAGAACGGTCGGCGCCGGTGTTGTCAGTAAAGTGATTGAATAG

Upstream 100 bases:

>100_bases
ATCATAAAAACGGTAAAATTATTTGATAGTAAAAGATCGTCCCACAAAAGGCTGGTAAATAACTTTTCAAAAGAAAGATA
AAGATTAAGGAGGTCGGAGG

Downstream 100 bases:

>100_bases
ATAGCGCATAAGTGATTGGATCAGACAAGGACATGAAAGATCAAAAAATAAGAATTCGCCTTAAGGCTTACGATTACAAA
TTACTTGATCGCTCGGTGGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKWLAKKGLAEFRAFDSIDNAPEEKERGVTINISHVEYQTDKRHYAHV
DCPGHADYIKNMISGAAHMDGTILVVAASDGPMPQTREHILLARQVQVPSIVVFLNKVDLVDDPELLDLVELELRELLNE
YEFPGDDIPIIRGSALKALESENPDDPDVKQIYALMDAVDAYIPEPERDLDKPFLMPVGDVFTISGRGTVVTGRIDRGII
KTGDEVEIVGVRPTQKTVCTGVEMFRKTLDEGRAGDDVGLLLRGIKREDVERGQVVAKPGSITPHTKFMAQVYVLTKEEG
GRHTPFFTGYRPQFYFRTTDVTGVAKLAEGVEMVMPGDNVEMEVTLITPIAMEEQLRFAIREGGRTVGAGVVSKVIE

Sequences:

>Translated_397_residues
MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKWLAKKGLAEFRAFDSIDNAPEEKERGVTINISHVEYQTDKRHYAHV
DCPGHADYIKNMISGAAHMDGTILVVAASDGPMPQTREHILLARQVQVPSIVVFLNKVDLVDDPELLDLVELELRELLNE
YEFPGDDIPIIRGSALKALESENPDDPDVKQIYALMDAVDAYIPEPERDLDKPFLMPVGDVFTISGRGTVVTGRIDRGII
KTGDEVEIVGVRPTQKTVCTGVEMFRKTLDEGRAGDDVGLLLRGIKREDVERGQVVAKPGSITPHTKFMAQVYVLTKEEG
GRHTPFFTGYRPQFYFRTTDVTGVAKLAEGVEMVMPGDNVEMEVTLITPIAMEEQLRFAIREGGRTVGAGVVSKVIE
>Mature_396_residues
AKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKWLAKKGLAEFRAFDSIDNAPEEKERGVTINISHVEYQTDKRHYAHVD
CPGHADYIKNMISGAAHMDGTILVVAASDGPMPQTREHILLARQVQVPSIVVFLNKVDLVDDPELLDLVELELRELLNEY
EFPGDDIPIIRGSALKALESENPDDPDVKQIYALMDAVDAYIPEPERDLDKPFLMPVGDVFTISGRGTVVTGRIDRGIIK
TGDEVEIVGVRPTQKTVCTGVEMFRKTLDEGRAGDDVGLLLRGIKREDVERGQVVAKPGSITPHTKFMAQVYVLTKEEGG
RHTPFFTGYRPQFYFRTTDVTGVAKLAEGVEMVMPGDNVEMEVTLITPIAMEEQLRFAIREGGRTVGAGVVSKVIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.4556962025316, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=30.5747126436782, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=30.5747126436782, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=29.3064876957494, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI4503475, Length=442, Percent_Identity=29.185520361991, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=30.3468208092486, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI194018522, Length=337, Percent_Identity=26.7062314540059, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI194097354, Length=337, Percent_Identity=26.7062314540059, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI194018520, Length=337, Percent_Identity=26.7062314540059, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI46094014, Length=326, Percent_Identity=26.3803680981595, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI4503507, Length=278, Percent_Identity=27.6978417266187, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157426893, Length=294, Percent_Identity=24.8299319727891, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=74.4949494949495, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=74.4949494949495, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI2367247, Length=384, Percent_Identity=29.6875, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=247, Percent_Identity=34.412955465587, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789108, Length=157, Percent_Identity=31.2101910828025, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788922, Length=142, Percent_Identity=35.2112676056338, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=52.8535980148883, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=44.559585492228, Blast_Score=347, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI17552884, Length=455, Percent_Identity=29.010989010989, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=455, Percent_Identity=29.010989010989, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=422, Percent_Identity=28.6729857819905, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=28.2110091743119, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=28.2110091743119, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=454, Percent_Identity=26.6519823788546, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=28.4671532846715, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=164, Percent_Identity=33.5365853658537, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17509919, Length=293, Percent_Identity=27.3037542662116, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=240, Percent_Identity=25.8333333333333, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=64.735516372796, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=28.8939051918736, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=28.8939051918736, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=454, Percent_Identity=26.6519823788546, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=344, Percent_Identity=27.3255813953488, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=286, Percent_Identity=28.3216783216783, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=282, Percent_Identity=28.0141843971631, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=141, Percent_Identity=32.6241134751773, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=56.5989847715736, Blast_Score=466, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=56.5989847715736, Blast_Score=466, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=49.6124031007752, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=443, Percent_Identity=28.6681715575621, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI45553816, Length=443, Percent_Identity=28.6681715575621, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24651721, Length=443, Percent_Identity=28.6681715575621, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI17864154, Length=443, Percent_Identity=28.6681715575621, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24652838, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17137572, Length=454, Percent_Identity=28.4140969162996, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=30.3921568627451, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=27.1264367816092, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17137380, Length=339, Percent_Identity=28.023598820059, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24647046, Length=288, Percent_Identity=27.7777777777778, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647048, Length=288, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=37.3493975903614, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_SYNAS (Q2LQA3)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_460346.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q2LQA3
- SMR:   Q2LQA3
- STRING:   Q2LQA3
- GeneID:   3884625
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_00983
- NMPDR:   fig|56780.10.peg.298
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- PhylomeDB:   Q2LQA3
- ProtClustDB:   PRK00049
- BioCyc:   SACI56780:SYN_00983-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43913; Mature: 43782

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKWLAKKGLAEFRAFDSIDNAPEEKERG
CCCCCCHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
VTINISHVEYQTDKRHYAHVDCPGHADYIKNMISGAAHMDGTILVVAASDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHH
LLARQVQVPSIVVFLNKVDLVDDPELLDLVELELRELLNEYEFPGDDIPIIRGSALKALE
HEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHH
SENPDDPDVKQIYALMDAVDAYIPEPERDLDKPFLMPVGDVFTISGRGTVVTGRIDRGII
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCCEEEECCCCEEEEECCCCCEE
KTGDEVEIVGVRPTQKTVCTGVEMFRKTLDEGRAGDDVGLLLRGIKREDVERGQVVAKPG
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHCCCEEEECCC
SITPHTKFMAQVYVLTKEEGGRHTPFFTGYRPQFYFRTTDVTGVAKLAEGVEMVMPGDNV
CCCCCHHEEEEEEEEEECCCCCCCCEECCCCCEEEEEECCCHHHHHHHCCCEEEECCCCC
EMEVTLITPIAMEEQLRFAIREGGRTVGAGVVSKVIE
EEEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKWLAKKGLAEFRAFDSIDNAPEEKERG
CCCCCHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
VTINISHVEYQTDKRHYAHVDCPGHADYIKNMISGAAHMDGTILVVAASDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHH
LLARQVQVPSIVVFLNKVDLVDDPELLDLVELELRELLNEYEFPGDDIPIIRGSALKALE
HEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHH
SENPDDPDVKQIYALMDAVDAYIPEPERDLDKPFLMPVGDVFTISGRGTVVTGRIDRGII
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCCEEEECCCCEEEEECCCCCEE
KTGDEVEIVGVRPTQKTVCTGVEMFRKTLDEGRAGDDVGLLLRGIKREDVERGQVVAKPG
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHCCCEEEECCC
SITPHTKFMAQVYVLTKEEGGRHTPFFTGYRPQFYFRTTDVTGVAKLAEGVEMVMPGDNV
CCCCCHHEEEEEEEEEECCCCCCCCEECCCCCEEEEEECCCHHHHHHHCCCEEEECCCCC
EMEVTLITPIAMEEQLRFAIREGGRTVGAGVVSKVIE
EEEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA